Labilibaculum antarcticum
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4329 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y1CMJ4|A0A1Y1CMJ4_9BACT Uncharacterized protein OS=Labilibaculum antarcticum OX=1717717 GN=ALGA_2938 PE=4 SV=1
MM1 pKa = 7.63 RR2 pKa = 11.84 LNWILISSLALLASTFACSDD22 pKa = 3.9 DD23 pKa = 3.77 DD24 pKa = 4.53 TISDD28 pKa = 4.17 SEE30 pKa = 4.68 NITEE34 pKa = 4.21 NLPDD38 pKa = 3.34 ISGYY42 pKa = 10.0 PIVGTNQSTAFNNTTNISTPSSGDD66 pKa = 3.17 DD67 pKa = 3.97 FYY69 pKa = 11.81 GQNANYY75 pKa = 9.74 PGTTPQYY82 pKa = 10.96 VDD84 pKa = 3.81 NGDD87 pKa = 3.62 GTVTDD92 pKa = 4.2 MVTGLMWQNTLDD104 pKa = 3.93 HH105 pKa = 6.84 NSDD108 pKa = 3.27 GLINYY113 pKa = 8.13 DD114 pKa = 4.58 DD115 pKa = 4.37 KK116 pKa = 11.35 LTYY119 pKa = 10.76 AEE121 pKa = 4.21 ILALASEE128 pKa = 4.85 VATGDD133 pKa = 3.55 YY134 pKa = 10.51 TDD136 pKa = 3.57 WRR138 pKa = 11.84 VPTIKK143 pKa = 10.21 EE144 pKa = 4.07 QYY146 pKa = 9.78 SLMMFSGRR154 pKa = 11.84 DD155 pKa = 3.12 ISGYY159 pKa = 10.41 EE160 pKa = 4.09 GTSTEE165 pKa = 4.07 GLTPFINTDD174 pKa = 3.41 YY175 pKa = 11.35 FGFNYY180 pKa = 10.46 GDD182 pKa = 3.26 TDD184 pKa = 3.37 AGEE187 pKa = 4.13 RR188 pKa = 11.84 LIDD191 pKa = 3.8 VQCASTNVSVGNTEE205 pKa = 3.44 IEE207 pKa = 4.27 MVFGVNFADD216 pKa = 5.03 GRR218 pKa = 11.84 IKK220 pKa = 10.9 GYY222 pKa = 8.32 GTTMMGQAKK231 pKa = 9.38 VFNYY235 pKa = 10.74 LMVRR239 pKa = 11.84 GNTTYY244 pKa = 11.33 GEE246 pKa = 4.4 NNFSDD251 pKa = 3.99 NADD254 pKa = 3.45 GTISDD259 pKa = 4.9 LATDD263 pKa = 4.48 LMWMQDD269 pKa = 3.68 DD270 pKa = 4.4 SDD272 pKa = 5.54 EE273 pKa = 4.44 GMNWQNALAYY283 pKa = 10.19 AEE285 pKa = 4.29 SFEE288 pKa = 4.37 FAGRR292 pKa = 11.84 SDD294 pKa = 3.04 WRR296 pKa = 11.84 LPSAKK301 pKa = 9.33 EE302 pKa = 3.63 LQSIVDD308 pKa = 3.83 YY309 pKa = 11.07 SRR311 pKa = 11.84 SPQSSASAAINPIFNCTQITNEE333 pKa = 4.23 AGEE336 pKa = 4.2 VDD338 pKa = 4.32 YY339 pKa = 10.71 PFYY342 pKa = 10.69 WSGTTHH348 pKa = 6.68 ATTGNDD354 pKa = 3.35 NNGGWGAYY362 pKa = 7.41 VAFGRR367 pKa = 11.84 AMGNEE372 pKa = 4.13 STILPDD378 pKa = 3.95 DD379 pKa = 3.81 QNPPTEE385 pKa = 4.79 GEE387 pKa = 4.16 QGPPPTDD394 pKa = 4.26 NIDD397 pKa = 3.56 SSSDD401 pKa = 3.61 SINWTDD407 pKa = 3.16 VHH409 pKa = 6.46 GAGAQRR415 pKa = 11.84 SDD417 pKa = 4.21 PKK419 pKa = 11.08 SGDD422 pKa = 3.22 PSEE425 pKa = 4.3 YY426 pKa = 10.64 SEE428 pKa = 4.22 GHH430 pKa = 6.1 GPQGDD435 pKa = 3.42 AVRR438 pKa = 11.84 ILNYY442 pKa = 9.57 IRR444 pKa = 11.84 LVRR447 pKa = 11.84 DD448 pKa = 4.02 TKK450 pKa = 11.28
Molecular weight: 48.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.692
IPC2_protein 3.795
IPC_protein 3.834
Toseland 3.605
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.656
Grimsley 3.503
Solomon 3.821
Lehninger 3.783
Nozaki 3.935
DTASelect 4.202
Thurlkill 3.656
EMBOSS 3.77
Sillero 3.948
Patrickios 1.1
IPC_peptide 3.821
IPC2_peptide 3.923
IPC2.peptide.svr19 3.835
Protein with the highest isoelectric point:
>tr|A0A1Y1CGP5|A0A1Y1CGP5_9BACT 50S ribosomal protein L4 OS=Labilibaculum antarcticum OX=1717717 GN=rplD PE=3 SV=1
MM1 pKa = 7.23 ARR3 pKa = 11.84 IVGVDD8 pKa = 3.58 LPQNKK13 pKa = 9.63 RR14 pKa = 11.84 GIISLTYY21 pKa = 8.96 IYY23 pKa = 10.73 GIGRR27 pKa = 11.84 SAARR31 pKa = 11.84 QILDD35 pKa = 3.62 EE36 pKa = 5.04 AEE38 pKa = 4.28 VPHH41 pKa = 7.04 DD42 pKa = 4.19 KK43 pKa = 10.38 QVKK46 pKa = 9.17 DD47 pKa = 3.3 WTDD50 pKa = 3.18 EE51 pKa = 3.69 EE52 pKa = 5.16 SNRR55 pKa = 11.84 IRR57 pKa = 11.84 QAINANLKK65 pKa = 10.27 VEE67 pKa = 4.2 GEE69 pKa = 4.12 LRR71 pKa = 11.84 SLNQLNIKK79 pKa = 10.09 RR80 pKa = 11.84 LMDD83 pKa = 3.77 IGCYY87 pKa = 9.44 RR88 pKa = 11.84 GIRR91 pKa = 11.84 HH92 pKa = 7.01 RR93 pKa = 11.84 IGLPVRR99 pKa = 11.84 GQRR102 pKa = 11.84 TKK104 pKa = 11.08 NNSRR108 pKa = 11.84 TRR110 pKa = 11.84 KK111 pKa = 9.2 GKK113 pKa = 10.5 RR114 pKa = 11.84 KK115 pKa = 7.64 TVANKK120 pKa = 10.07 KK121 pKa = 9.89 KK122 pKa = 9.38 ATKK125 pKa = 10.14
Molecular weight: 14.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.312
IPC2_protein 9.838
IPC_protein 10.701
Toseland 11.067
ProMoST 10.877
Dawson 11.111
Bjellqvist 10.833
Wikipedia 11.345
Rodwell 11.359
Grimsley 11.14
Solomon 11.301
Lehninger 11.257
Nozaki 11.038
DTASelect 10.833
Thurlkill 11.038
EMBOSS 11.477
Sillero 11.052
Patrickios 11.082
IPC_peptide 11.316
IPC2_peptide 9.75
IPC2.peptide.svr19 8.635
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4329
0
4329
1607196
42
4359
371.3
41.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.234 ± 0.04
0.966 ± 0.013
5.691 ± 0.033
6.854 ± 0.038
5.067 ± 0.027
6.59 ± 0.041
1.757 ± 0.017
7.992 ± 0.036
7.706 ± 0.045
9.329 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.412 ± 0.021
5.854 ± 0.035
3.295 ± 0.019
3.321 ± 0.02
3.555 ± 0.022
6.843 ± 0.037
5.173 ± 0.044
6.167 ± 0.031
1.153 ± 0.016
4.04 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here