Sphingobium sp. LB126

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingobium; unclassified Sphingobium

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4890 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2M8QQN3|A0A2M8QQN3_9SPHN Coenzyme A biosynthesis bifunctional protein CoaBC OS=Sphingobium sp. LB126 OX=1983755 GN=coaBC PE=3 SV=1
MM1 pKa = 7.26RR2 pKa = 11.84TTYY5 pKa = 11.1NSATVRR11 pKa = 11.84LYY13 pKa = 10.77HH14 pKa = 7.18LSDD17 pKa = 3.9ADD19 pKa = 3.63GGGAATTLFYY29 pKa = 11.34GPLNEE34 pKa = 4.59ALLLAEE40 pKa = 4.57QQPQDD45 pKa = 3.68MQDD48 pKa = 3.55GLFLATDD55 pKa = 3.84NDD57 pKa = 3.68VVAYY61 pKa = 10.54LDD63 pKa = 4.69LIEE66 pKa = 4.37GG67 pKa = 3.86

Molecular weight: 7.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2M8QTF8|A0A2M8QTF8_9SPHN Nuclease OS=Sphingobium sp. LB126 OX=1983755 GN=CAF53_11715 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84SRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.06SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4890

0

4890

1551164

29

3228

317.2

34.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.935 ± 0.05

0.807 ± 0.011

5.959 ± 0.028

5.377 ± 0.035

3.515 ± 0.021

8.802 ± 0.04

2.127 ± 0.018

5.185 ± 0.021

2.998 ± 0.024

10.022 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.576 ± 0.02

2.565 ± 0.026

5.273 ± 0.025

3.261 ± 0.019

7.468 ± 0.04

5.362 ± 0.028

5.118 ± 0.03

6.969 ± 0.032

1.424 ± 0.014

2.246 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski