Amino acid dipepetide frequency for Sphingobium sp. LB126

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.106AlaAla: 18.106 ± 0.144	1.068AlaCys: 1.068 ± 0.027	7.55AlaAsp: 7.55 ± 0.073	7.24AlaGlu: 7.24 ± 0.075	4.339AlaPhe: 4.339 ± 0.062	10.998AlaGly: 10.998 ± 0.098	2.476AlaHis: 2.476 ± 0.048	6.817AlaIle: 6.817 ± 0.074	3.921AlaLys: 3.921 ± 0.064	13.955AlaLeu: 13.955 ± 0.126	4.034AlaMet: 4.034 ± 0.054	3.075AlaAsn: 3.075 ± 0.046	6.027AlaPro: 6.027 ± 0.062	4.72AlaGln: 4.72 ± 0.056	9.729AlaArg: 9.729 ± 0.092	6.537AlaSer: 6.537 ± 0.082	6.204AlaThr: 6.204 ± 0.074	8.432AlaVal: 8.432 ± 0.085	1.516AlaTrp: 1.516 ± 0.032	2.599AlaTyr: 2.599 ± 0.039	0.009AlaXaa: 0.009 ± 0.003
Cys	1.049CysAla: 1.049 ± 0.032	0.091CysCys: 0.091 ± 0.008	0.513CysAsp: 0.513 ± 0.019	0.393CysGlu: 0.393 ± 0.018	0.286CysPhe: 0.286 ± 0.016	0.874CysGly: 0.874 ± 0.025	0.233CysHis: 0.233 ± 0.017	0.358CysIle: 0.358 ± 0.017	0.164CysLys: 0.164 ± 0.011	0.73CysLeu: 0.73 ± 0.025	0.172CysMet: 0.172 ± 0.011	0.197CysAsn: 0.197 ± 0.011	0.458CysPro: 0.458 ± 0.017	0.217CysGln: 0.217 ± 0.012	0.596CysArg: 0.596 ± 0.02	0.46CysSer: 0.46 ± 0.018	0.397CysThr: 0.397 ± 0.018	0.559CysVal: 0.559 ± 0.02	0.139CysTrp: 0.139 ± 0.009	0.182CysTyr: 0.182 ± 0.011	0.001CysXaa: 0.001 ± 0.001
Asp	7.422AspAla: 7.422 ± 0.081	0.504AspCys: 0.504 ± 0.02	3.235AspAsp: 3.235 ± 0.051	3.304AspGlu: 3.304 ± 0.053	2.157AspPhe: 2.157 ± 0.04	5.566AspGly: 5.566 ± 0.076	1.487AspHis: 1.487 ± 0.032	3.161AspIle: 3.161 ± 0.049	1.605AspLys: 1.605 ± 0.041	5.902AspLeu: 5.902 ± 0.068	1.641AspMet: 1.641 ± 0.028	1.379AspAsn: 1.379 ± 0.033	3.72AspPro: 3.72 ± 0.049	1.927AspGln: 1.927 ± 0.031	5.103AspArg: 5.103 ± 0.066	2.423AspSer: 2.423 ± 0.045	2.363AspThr: 2.363 ± 0.047	3.915AspVal: 3.915 ± 0.051	1.073AspTrp: 1.073 ± 0.026	1.702AspTyr: 1.702 ± 0.04	0.003AspXaa: 0.003 ± 0.002
Glu	7.582GluAla: 7.582 ± 0.091	0.336GluCys: 0.336 ± 0.014	2.748GluAsp: 2.748 ± 0.049	3.124GluGlu: 3.124 ± 0.055	1.435GluPhe: 1.435 ± 0.032	4.616GluGly: 4.616 ± 0.066	1.111GluHis: 1.111 ± 0.031	3.003GluIle: 3.003 ± 0.044	2.074GluLys: 2.074 ± 0.039	4.892GluLeu: 4.892 ± 0.063	1.468GluMet: 1.468 ± 0.031	1.391GluAsn: 1.391 ± 0.028	2.454GluPro: 2.454 ± 0.04	2.104GluGln: 2.104 ± 0.042	4.952GluArg: 4.952 ± 0.06	2.299GluSer: 2.299 ± 0.042	3.064GluThr: 3.064 ± 0.043	3.435GluVal: 3.435 ± 0.052	0.748GluTrp: 0.748 ± 0.021	0.924GluTyr: 0.924 ± 0.025	0.006GluXaa: 0.006 ± 0.002
Phe	4.64PheAla: 4.64 ± 0.055	0.364PheCys: 0.364 ± 0.016	2.587PheAsp: 2.587 ± 0.047	1.927PheGlu: 1.927 ± 0.037	1.256PhePhe: 1.256 ± 0.038	3.52PheGly: 3.52 ± 0.051	0.774PheHis: 0.774 ± 0.025	1.509PheIle: 1.509 ± 0.037	0.841PheLys: 0.841 ± 0.025	3.229PheLeu: 3.229 ± 0.051	0.744PheMet: 0.744 ± 0.023	0.998PheAsn: 0.998 ± 0.028	1.556PhePro: 1.556 ± 0.032	0.958PheGln: 0.958 ± 0.023	2.287PheArg: 2.287 ± 0.043	2.1PheSer: 2.1 ± 0.04	2.02PheThr: 2.02 ± 0.043	2.426PheVal: 2.426 ± 0.04	0.534PheTrp: 0.534 ± 0.02	0.876PheTyr: 0.876 ± 0.025	0.003PheXaa: 0.003 ± 0.001
Gly	9.676GlyAla: 9.676 ± 0.091	0.809GlyCys: 0.809 ± 0.029	4.77GlyAsp: 4.77 ± 0.064	4.795GlyGlu: 4.795 ± 0.059	3.566GlyPhe: 3.566 ± 0.054	7.89GlyGly: 7.89 ± 0.105	1.899GlyHis: 1.899 ± 0.038	4.553GlyIle: 4.553 ± 0.06	3.262GlyLys: 3.262 ± 0.058	8.834GlyLeu: 8.834 ± 0.087	2.418GlyMet: 2.418 ± 0.043	2.34GlyAsn: 2.34 ± 0.055	3.676GlyPro: 3.676 ± 0.06	3.202GlyGln: 3.202 ± 0.043	6.687GlyArg: 6.687 ± 0.062	4.887GlySer: 4.887 ± 0.069	4.683GlyThr: 4.683 ± 0.081	6.114GlyVal: 6.114 ± 0.064	1.605GlyTrp: 1.605 ± 0.036	2.349GlyTyr: 2.349 ± 0.043	0.008GlyXaa: 0.008 ± 0.003
His	2.512HisAla: 2.512 ± 0.05	0.233HisCys: 0.233 ± 0.012	1.298HisAsp: 1.298 ± 0.031	1.021HisGlu: 1.021 ± 0.028	0.924HisPhe: 0.924 ± 0.024	2.132HisGly: 2.132 ± 0.038	0.675HisHis: 0.675 ± 0.025	1.021HisIle: 1.021 ± 0.026	0.467HisLys: 0.467 ± 0.016	2.078HisLeu: 2.078 ± 0.038	0.551HisMet: 0.551 ± 0.019	0.496HisAsn: 0.496 ± 0.018	1.319HisPro: 1.319 ± 0.03	0.617HisGln: 0.617 ± 0.02	1.578HisArg: 1.578 ± 0.036	1.02HisSer: 1.02 ± 0.025	0.638HisThr: 0.638 ± 0.023	1.616HisVal: 1.616 ± 0.037	0.405HisTrp: 0.405 ± 0.016	0.665HisTyr: 0.665 ± 0.021	0.003HisXaa: 0.003 ± 0.002
Ile	7.836IleAla: 7.836 ± 0.08	0.465IleCys: 0.465 ± 0.018	3.965IleAsp: 3.965 ± 0.057	3.479IleGlu: 3.479 ± 0.049	1.686IlePhe: 1.686 ± 0.032	5.083IleGly: 5.083 ± 0.061	0.975IleHis: 0.975 ± 0.024	2.256IleIle: 2.256 ± 0.047	1.193IleLys: 1.193 ± 0.031	4.453IleLeu: 4.453 ± 0.058	1.019IleMet: 1.019 ± 0.025	1.335IleAsn: 1.335 ± 0.029	2.353IlePro: 2.353 ± 0.043	1.238IleGln: 1.238 ± 0.029	3.376IleArg: 3.376 ± 0.051	2.808IleSer: 2.808 ± 0.041	2.515IleThr: 2.515 ± 0.045	4.099IleVal: 4.099 ± 0.055	0.655IleTrp: 0.655 ± 0.022	1.057IleTyr: 1.057 ± 0.026	0.002IleXaa: 0.002 ± 0.001
Lys	4.197LysAla: 4.197 ± 0.066	0.143LysCys: 0.143 ± 0.009	1.623LysAsp: 1.623 ± 0.041	1.318LysGlu: 1.318 ± 0.029	0.788LysPhe: 0.788 ± 0.023	2.73LysGly: 2.73 ± 0.047	0.494LysHis: 0.494 ± 0.018	1.487LysIle: 1.487 ± 0.034	1.044LysLys: 1.044 ± 0.028	3.147LysLeu: 3.147 ± 0.052	0.737LysMet: 0.737 ± 0.024	0.741LysAsn: 0.741 ± 0.023	1.96LysPro: 1.96 ± 0.045	0.906LysGln: 0.906 ± 0.023	2.281LysArg: 2.281 ± 0.034	1.589LysSer: 1.589 ± 0.034	1.641LysThr: 1.641 ± 0.038	2.181LysVal: 2.181 ± 0.039	0.404LysTrp: 0.404 ± 0.015	0.567LysTyr: 0.567 ± 0.022	0.001LysXaa: 0.001 ± 0.001
Leu	13.708LeuAla: 13.708 ± 0.124	0.91LeuCys: 0.91 ± 0.027	6.165LeuAsp: 6.165 ± 0.061	4.852LeuGlu: 4.852 ± 0.054	3.76LeuPhe: 3.76 ± 0.055	8.204LeuGly: 8.204 ± 0.083	1.986LeuHis: 1.986 ± 0.037	5.127LeuIle: 5.127 ± 0.069	3.118LeuLys: 3.118 ± 0.05	9.866LeuLeu: 9.866 ± 0.128	2.329LeuMet: 2.329 ± 0.037	2.521LeuAsn: 2.521 ± 0.046	5.667LeuPro: 5.667 ± 0.069	2.539LeuGln: 2.539 ± 0.045	7.128LeuArg: 7.128 ± 0.084	6.471LeuSer: 6.471 ± 0.063	5.588LeuThr: 5.588 ± 0.069	6.973LeuVal: 6.973 ± 0.077	1.3LeuTrp: 1.3 ± 0.035	2.008LeuTyr: 2.008 ± 0.035	0.004LeuXaa: 0.004 ± 0.002
Met	3.525MetAla: 3.525 ± 0.056	0.148MetCys: 0.148 ± 0.01	1.234MetAsp: 1.234 ± 0.035	1.188MetGlu: 1.188 ± 0.033	0.689MetPhe: 0.689 ± 0.022	2.024MetGly: 2.024 ± 0.037	0.496MetHis: 0.496 ± 0.018	1.432MetIle: 1.432 ± 0.032	0.957MetLys: 0.957 ± 0.025	2.801MetLeu: 2.801 ± 0.044	0.733MetMet: 0.733 ± 0.024	0.725MetAsn: 0.725 ± 0.023	1.563MetPro: 1.563 ± 0.03	0.821MetGln: 0.821 ± 0.023	1.877MetArg: 1.877 ± 0.036	1.5MetSer: 1.5 ± 0.03	1.875MetThr: 1.875 ± 0.037	1.699MetVal: 1.699 ± 0.034	0.228MetTrp: 0.228 ± 0.012	0.242MetTyr: 0.242 ± 0.012	0.005MetXaa: 0.005 ± 0.003
Asn	3.292AsnAla: 3.292 ± 0.059	0.22AsnCys: 0.22 ± 0.012	1.489AsnAsp: 1.489 ± 0.05	1.111AsnGlu: 1.111 ± 0.032	0.92AsnPhe: 0.92 ± 0.025	2.575AsnGly: 2.575 ± 0.052	0.505AsnHis: 0.505 ± 0.017	1.373AsnIle: 1.373 ± 0.033	0.664AsnLys: 0.664 ± 0.019	2.464AsnLeu: 2.464 ± 0.042	0.645AsnMet: 0.645 ± 0.019	0.681AsnAsn: 0.681 ± 0.023	1.693AsnPro: 1.693 ± 0.036	0.785AsnGln: 0.785 ± 0.023	1.926AsnArg: 1.926 ± 0.038	1.298AsnSer: 1.298 ± 0.033	0.98AsnThr: 0.98 ± 0.032	1.864AsnVal: 1.864 ± 0.034	0.444AsnTrp: 0.444 ± 0.017	0.726AsnTyr: 0.726 ± 0.025	0.002AsnXaa: 0.002 ± 0.001
Pro	6.754ProAla: 6.754 ± 0.069	0.338ProCys: 0.338 ± 0.017	3.847ProAsp: 3.847 ± 0.054	3.357ProGlu: 3.357 ± 0.044	1.928ProPhe: 1.928 ± 0.033	4.694ProGly: 4.694 ± 0.063	1.155ProHis: 1.155 ± 0.028	2.472ProIle: 2.472 ± 0.039	1.473ProLys: 1.473 ± 0.031	4.941ProLeu: 4.941 ± 0.057	1.3ProMet: 1.3 ± 0.024	1.289ProAsn: 1.289 ± 0.031	2.769ProPro: 2.769 ± 0.056	1.737ProGln: 1.737 ± 0.036	3.022ProArg: 3.022 ± 0.045	2.868ProSer: 2.868 ± 0.045	2.579ProThr: 2.579 ± 0.046	4.394ProVal: 4.394 ± 0.055	0.699ProTrp: 0.699 ± 0.026	1.111ProTyr: 1.111 ± 0.028	0.007ProXaa: 0.007 ± 0.003
Gln	4.501GlnAla: 4.501 ± 0.066	0.225GlnCys: 0.225 ± 0.013	1.574GlnAsp: 1.574 ± 0.034	1.439GlnGlu: 1.439 ± 0.032	1.07GlnPhe: 1.07 ± 0.026	2.675GlnGly: 2.675 ± 0.044	0.63GlnHis: 0.63 ± 0.023	1.908GlnIle: 1.908 ± 0.036	1.002GlnLys: 1.002 ± 0.028	3.142GlnLeu: 3.142 ± 0.046	0.921GlnMet: 0.921 ± 0.024	0.803GlnAsn: 0.803 ± 0.026	1.96GlnPro: 1.96 ± 0.038	1.285GlnGln: 1.285 ± 0.036	2.613GlnArg: 2.613 ± 0.048	1.829GlnSer: 1.829 ± 0.034	1.64GlnThr: 1.64 ± 0.037	2.245GlnVal: 2.245 ± 0.045	0.48GlnTrp: 0.48 ± 0.017	0.665GlnTyr: 0.665 ± 0.023	0.003GlnXaa: 0.003 ± 0.002
Arg	8.618ArgAla: 8.618 ± 0.085	0.537ArgCys: 0.537 ± 0.019	4.39ArgAsp: 4.39 ± 0.065	4.021ArgGlu: 4.021 ± 0.059	3.034ArgPhe: 3.034 ± 0.041	5.027ArgGly: 5.027 ± 0.063	1.957ArgHis: 1.957 ± 0.041	4.39ArgIle: 4.39 ± 0.057	2.267ArgLys: 2.267 ± 0.044	8.57ArgLeu: 8.57 ± 0.09	1.982ArgMet: 1.982 ± 0.036	1.968ArgAsn: 1.968 ± 0.038	3.879ArgPro: 3.879 ± 0.052	2.791ArgGln: 2.791 ± 0.045	5.992ArgArg: 5.992 ± 0.091	3.869ArgSer: 3.869 ± 0.048	3.65ArgThr: 3.65 ± 0.047	4.523ArgVal: 4.523 ± 0.054	1.251ArgTrp: 1.251 ± 0.031	1.95ArgTyr: 1.95 ± 0.033	0.012ArgXaa: 0.012 ± 0.004
Ser	6.551SerAla: 6.551 ± 0.073	0.457SerCys: 0.457 ± 0.018	3.115SerAsp: 3.115 ± 0.043	2.556SerGlu: 2.556 ± 0.044	2.231SerPhe: 2.231 ± 0.043	5.587SerGly: 5.587 ± 0.068	1.073SerHis: 1.073 ± 0.027	2.844SerIle: 2.844 ± 0.043	1.391SerLys: 1.391 ± 0.031	5.395SerLeu: 5.395 ± 0.064	1.322SerMet: 1.322 ± 0.028	1.351SerAsn: 1.351 ± 0.032	2.822SerPro: 2.822 ± 0.045	1.65SerGln: 1.65 ± 0.033	3.688SerArg: 3.688 ± 0.051	2.934SerSer: 2.934 ± 0.057	2.691SerThr: 2.691 ± 0.045	3.722SerVal: 3.722 ± 0.048	0.808SerTrp: 0.808 ± 0.022	1.429SerTyr: 1.429 ± 0.033	0.003SerXaa: 0.003 ± 0.002
Thr	6.201ThrAla: 6.201 ± 0.08	0.368ThrCys: 0.368 ± 0.015	2.833ThrAsp: 2.833 ± 0.05	2.298ThrGlu: 2.298 ± 0.041	1.676ThrPhe: 1.676 ± 0.037	5.227ThrGly: 5.227 ± 0.068	1.028ThrHis: 1.028 ± 0.028	2.975ThrIle: 2.975 ± 0.047	1.293ThrLys: 1.293 ± 0.032	5.787ThrLeu: 5.787 ± 0.063	1.175ThrMet: 1.175 ± 0.033	1.258ThrAsn: 1.258 ± 0.036	3.324ThrPro: 3.324 ± 0.05	1.564ThrGln: 1.564 ± 0.038	3.305ThrArg: 3.305 ± 0.04	2.574ThrSer: 2.574 ± 0.043	2.512ThrThr: 2.512 ± 0.05	4.083ThrVal: 4.083 ± 0.062	0.56ThrTrp: 0.56 ± 0.019	1.133ThrTyr: 1.133 ± 0.035	0.006ThrXaa: 0.006 ± 0.002
Val	9.118ValAla: 9.118 ± 0.08	0.5ValCys: 0.5 ± 0.02	4.367ValAsp: 4.367 ± 0.061	4.573ValGlu: 4.573 ± 0.059	2.023ValPhe: 2.023 ± 0.037	5.448ValGly: 5.448 ± 0.061	1.405ValHis: 1.405 ± 0.03	3.532ValIle: 3.532 ± 0.047	2.156ValLys: 2.156 ± 0.044	6.155ValLeu: 6.155 ± 0.071	1.697ValMet: 1.697 ± 0.036	1.967ValAsn: 1.967 ± 0.039	3.802ValPro: 3.802 ± 0.053	2.216ValGln: 2.216 ± 0.04	5.204ValArg: 5.204 ± 0.061	3.971ValSer: 3.971 ± 0.049	4.335ValThr: 4.335 ± 0.068	5.067ValVal: 5.067 ± 0.072	0.781ValTrp: 0.781 ± 0.023	1.374ValTyr: 1.374 ± 0.033	0.005ValXaa: 0.005 ± 0.002
Trp	1.387TrpAla: 1.387 ± 0.028	0.112TrpCys: 0.112 ± 0.009	0.708TrpAsp: 0.708 ± 0.021	0.625TrpGlu: 0.625 ± 0.02	0.527TrpPhe: 0.527 ± 0.02	0.963TrpGly: 0.963 ± 0.027	0.363TrpHis: 0.363 ± 0.014	0.739TrpIle: 0.739 ± 0.024	0.501TrpLys: 0.501 ± 0.017	1.743TrpLeu: 1.743 ± 0.037	0.4TrpMet: 0.4 ± 0.016	0.484TrpAsn: 0.484 ± 0.016	0.685TrpPro: 0.685 ± 0.02	0.597TrpGln: 0.597 ± 0.022	1.358TrpArg: 1.358 ± 0.03	0.883TrpSer: 0.883 ± 0.03	0.853TrpThr: 0.853 ± 0.023	0.791TrpVal: 0.791 ± 0.024	0.246TrpTrp: 0.246 ± 0.014	0.277TrpTyr: 0.277 ± 0.012	0.002TrpXaa: 0.002 ± 0.001
Tyr	2.667TyrAla: 2.667 ± 0.04	0.241TyrCys: 0.241 ± 0.014	1.578TyrAsp: 1.578 ± 0.036	1.14TyrGlu: 1.14 ± 0.031	0.848TyrPhe: 0.848 ± 0.023	2.18TyrGly: 2.18 ± 0.044	0.543TyrHis: 0.543 ± 0.02	0.886TyrIle: 0.886 ± 0.023	0.585TyrLys: 0.585 ± 0.02	2.129TyrLeu: 2.129 ± 0.042	0.471TyrMet: 0.471 ± 0.019	0.656TyrAsn: 0.656 ± 0.023	1.044TyrPro: 1.044 ± 0.028	0.733TyrGln: 0.733 ± 0.023	1.967TyrArg: 1.967 ± 0.042	1.298TyrSer: 1.298 ± 0.03	0.953TyrThr: 0.953 ± 0.026	1.554TyrVal: 1.554 ± 0.03	0.364TyrTrp: 0.364 ± 0.018	0.619TyrTyr: 0.619 ± 0.021	0.002TyrXaa: 0.002 ± 0.001
Xaa	0.008XaaAla: 0.008 ± 0.003	0.001XaaCys: 0.001 ± 0.001	0.005XaaAsp: 0.005 ± 0.002	0.004XaaGlu: 0.004 ± 0.002	0.004XaaPhe: 0.004 ± 0.002	0.011XaaGly: 0.011 ± 0.004	0.001XaaHis: 0.001 ± 0.001	0.005XaaIle: 0.005 ± 0.002	0.002XaaLys: 0.002 ± 0.001	0.009XaaLeu: 0.009 ± 0.004	0.004XaaMet: 0.004 ± 0.002	0.001XaaAsn: 0.001 ± 0.001	0.004XaaPro: 0.004 ± 0.002	0.004XaaGln: 0.004 ± 0.002	0.008XaaArg: 0.008 ± 0.003	0.003XaaSer: 0.003 ± 0.001	0.003XaaThr: 0.003 ± 0.002	0.005XaaVal: 0.005 ± 0.002	0.003XaaTrp: 0.003 ± 0.001	0.003XaaTyr: 0.003 ± 0.001	0.006XaaXaa: 0.006 ± 0.002

Statistics based on 4890 proteins (1551164 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---