Salmonella phage S124
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 156 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5HS80|A0A2Z5HS80_9CAUD T5orf172 domain-containing protein OS=Salmonella phage S124 OX=2231351 PE=4 SV=1
MM1 pKa = 7.51 NIVLPKK7 pKa = 10.25 TLDD10 pKa = 2.99 KK11 pKa = 10.78 KK12 pKa = 10.99 QYY14 pKa = 8.27 ITPDD18 pKa = 3.18 DD19 pKa = 3.96 MEE21 pKa = 5.2 CGKK24 pKa = 10.62 VYY26 pKa = 10.73 QFDD29 pKa = 4.02 YY30 pKa = 10.83 MLASPTNTGCIIAVDD45 pKa = 3.67 TRR47 pKa = 11.84 DD48 pKa = 3.58 NDD50 pKa = 3.56 VLTFNDD56 pKa = 3.68 YY57 pKa = 11.38 SDD59 pKa = 3.65 FMEE62 pKa = 4.4 WLDD65 pKa = 4.7 DD66 pKa = 4.49 QSITNNGEE74 pKa = 3.68 LTPYY78 pKa = 10.78 QLTLTIKK85 pKa = 10.58
Molecular weight: 9.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.91
IPC_protein 3.859
Toseland 3.63
ProMoST 4.062
Dawson 3.872
Bjellqvist 4.037
Wikipedia 3.859
Rodwell 3.694
Grimsley 3.554
Solomon 3.859
Lehninger 3.821
Nozaki 3.999
DTASelect 4.279
Thurlkill 3.719
EMBOSS 3.859
Sillero 3.986
Patrickios 1.939
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.887
Protein with the highest isoelectric point:
>tr|A0A2Z5HTE6|A0A2Z5HTE6_9CAUD Uncharacterized protein OS=Salmonella phage S124 OX=2231351 PE=4 SV=1
MM1 pKa = 7.79 AKK3 pKa = 9.77 QKK5 pKa = 10.18 NAKK8 pKa = 7.58 TQAAPAVKK16 pKa = 9.52 TFPQTEE22 pKa = 4.31 ANRR25 pKa = 11.84 KK26 pKa = 8.9 ARR28 pKa = 11.84 LEE30 pKa = 3.84 RR31 pKa = 11.84 HH32 pKa = 5.4 LRR34 pKa = 11.84 KK35 pKa = 9.98 HH36 pKa = 5.57 PTDD39 pKa = 3.56 TQAQAALTAPLPVRR53 pKa = 11.84 HH54 pKa = 6.14 KK55 pKa = 10.48 PKK57 pKa = 10.6 AKK59 pKa = 9.96 NSTRR63 pKa = 11.84 ATAKK67 pKa = 9.91 HH68 pKa = 5.03 VVFVPGEE75 pKa = 4.0 GHH77 pKa = 6.47 KK78 pKa = 10.51 SVPVTLSFNGGAEE91 pKa = 4.01 LFARR95 pKa = 11.84 KK96 pKa = 10.21 DD97 pKa = 3.63 MTPKK101 pKa = 10.29 EE102 pKa = 4.06 YY103 pKa = 10.89 EE104 pKa = 3.81 KK105 pKa = 11.11 VVNQKK110 pKa = 9.68 RR111 pKa = 11.84 KK112 pKa = 9.61 PMADD116 pKa = 2.87 VMRR119 pKa = 11.84 EE120 pKa = 3.73 SRR122 pKa = 11.84 GQFGSVKK129 pKa = 9.73 PNIFGVEE136 pKa = 3.77 YY137 pKa = 10.96 SRR139 pKa = 11.84 DD140 pKa = 3.43 NVRR143 pKa = 11.84 ALCYY147 pKa = 10.52 GIGIKK152 pKa = 9.02 FTGASDD158 pKa = 3.56 RR159 pKa = 11.84 KK160 pKa = 9.12 SAKK163 pKa = 9.32 PARR166 pKa = 11.84 KK167 pKa = 9.36 RR168 pKa = 11.84 KK169 pKa = 9.78 AKK171 pKa = 10.37
Molecular weight: 18.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.264
IPC2_protein 9.853
IPC_protein 10.35
Toseland 11.067
ProMoST 10.687
Dawson 11.111
Bjellqvist 10.76
Wikipedia 11.286
Rodwell 11.55
Grimsley 11.14
Solomon 11.242
Lehninger 11.213
Nozaki 11.038
DTASelect 10.76
Thurlkill 11.038
EMBOSS 11.462
Sillero 11.052
Patrickios 11.272
IPC_peptide 11.257
IPC2_peptide 9.37
IPC2.peptide.svr19 8.678
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
156
0
156
32171
40
1226
206.2
23.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.603 ± 0.379
1.141 ± 0.107
6.282 ± 0.16
7.056 ± 0.222
4.06 ± 0.169
6.54 ± 0.291
2.03 ± 0.146
6.608 ± 0.162
7.112 ± 0.234
8.327 ± 0.21
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.683 ± 0.14
5.235 ± 0.185
3.453 ± 0.126
3.693 ± 0.209
4.715 ± 0.139
6.114 ± 0.17
5.766 ± 0.19
6.338 ± 0.163
1.278 ± 0.095
3.966 ± 0.136
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here