Amino acid dipepetide frequency for Salmonella phage S124

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.523AlaAla: 7.523 ± 1.029
0.591AlaCys: 0.591 ± 0.116
4.414AlaAsp: 4.414 ± 0.438
5.378AlaGlu: 5.378 ± 0.448
2.984AlaPhe: 2.984 ± 0.241
5.191AlaGly: 5.191 ± 0.555
1.337AlaHis: 1.337 ± 0.212
4.787AlaIle: 4.787 ± 0.372
6.403AlaLys: 6.403 ± 0.525
6.217AlaLeu: 6.217 ± 0.532
1.741AlaMet: 1.741 ± 0.265
3.513AlaAsn: 3.513 ± 0.364
2.425AlaPro: 2.425 ± 0.331
3.45AlaGln: 3.45 ± 0.501
3.419AlaArg: 3.419 ± 0.309
4.632AlaSer: 4.632 ± 0.569
3.761AlaThr: 3.761 ± 0.426
4.663AlaVal: 4.663 ± 0.47
0.964AlaTrp: 0.964 ± 0.165
2.642AlaTyr: 2.642 ± 0.313
0.0AlaXaa: 0.0 ± 0.0
Cys
0.715CysAla: 0.715 ± 0.143
0.311CysCys: 0.311 ± 0.11
0.435CysAsp: 0.435 ± 0.132
0.839CysGlu: 0.839 ± 0.16
0.373CysPhe: 0.373 ± 0.11
1.119CysGly: 1.119 ± 0.227
0.373CysHis: 0.373 ± 0.124
0.591CysIle: 0.591 ± 0.13
0.746CysLys: 0.746 ± 0.15
0.87CysLeu: 0.87 ± 0.169
0.187CysMet: 0.187 ± 0.08
0.622CysAsn: 0.622 ± 0.142
0.684CysPro: 0.684 ± 0.171
0.435CysGln: 0.435 ± 0.127
0.435CysArg: 0.435 ± 0.107
0.995CysSer: 0.995 ± 0.173
0.528CysThr: 0.528 ± 0.125
0.653CysVal: 0.653 ± 0.159
0.187CysTrp: 0.187 ± 0.077
0.311CysTyr: 0.311 ± 0.089
0.0CysXaa: 0.0 ± 0.0
Asp
4.321AspAla: 4.321 ± 0.413
0.622AspCys: 0.622 ± 0.135
3.046AspAsp: 3.046 ± 0.386
4.911AspGlu: 4.911 ± 0.425
2.86AspPhe: 2.86 ± 0.291
3.886AspGly: 3.886 ± 0.376
1.399AspHis: 1.399 ± 0.206
4.694AspIle: 4.694 ± 0.427
4.383AspLys: 4.383 ± 0.365
5.44AspLeu: 5.44 ± 0.492
2.176AspMet: 2.176 ± 0.225
3.171AspAsn: 3.171 ± 0.312
2.487AspPro: 2.487 ± 0.248
1.554AspGln: 1.554 ± 0.229
3.015AspArg: 3.015 ± 0.325
3.544AspSer: 3.544 ± 0.393
3.575AspThr: 3.575 ± 0.343
4.103AspVal: 4.103 ± 0.333
0.777AspTrp: 0.777 ± 0.167
2.86AspTyr: 2.86 ± 0.31
0.0AspXaa: 0.0 ± 0.0
Glu
6.186GluAla: 6.186 ± 0.508
0.808GluCys: 0.808 ± 0.195
3.792GluAsp: 3.792 ± 0.407
5.595GluGlu: 5.595 ± 0.526
2.425GluPhe: 2.425 ± 0.274
4.103GluGly: 4.103 ± 0.324
1.554GluHis: 1.554 ± 0.214
5.036GluIle: 5.036 ± 0.424
4.601GluLys: 4.601 ± 0.361
7.212GluLeu: 7.212 ± 0.437
2.331GluMet: 2.331 ± 0.261
2.083GluAsn: 2.083 ± 0.262
1.772GluPro: 1.772 ± 0.246
2.891GluGln: 2.891 ± 0.341
3.388GluArg: 3.388 ± 0.342
3.606GluSer: 3.606 ± 0.315
4.01GluThr: 4.01 ± 0.298
4.694GluVal: 4.694 ± 0.36
1.212GluTrp: 1.212 ± 0.186
3.264GluTyr: 3.264 ± 0.307
0.0GluXaa: 0.0 ± 0.0
Phe
2.456PheAla: 2.456 ± 0.273
0.28PheCys: 0.28 ± 0.111
2.735PheAsp: 2.735 ± 0.31
3.046PheGlu: 3.046 ± 0.317
1.554PhePhe: 1.554 ± 0.213
2.611PheGly: 2.611 ± 0.326
0.964PheHis: 0.964 ± 0.165
2.829PheIle: 2.829 ± 0.291
3.015PheLys: 3.015 ± 0.286
2.984PheLeu: 2.984 ± 0.335
0.87PheMet: 0.87 ± 0.194
2.922PheAsn: 2.922 ± 0.325
1.679PhePro: 1.679 ± 0.256
1.181PheGln: 1.181 ± 0.201
1.834PheArg: 1.834 ± 0.226
2.891PheSer: 2.891 ± 0.267
2.083PheThr: 2.083 ± 0.263
2.58PheVal: 2.58 ± 0.259
0.622PheTrp: 0.622 ± 0.137
1.461PheTyr: 1.461 ± 0.185
0.0PheXaa: 0.0 ± 0.0
Gly
4.445GlyAla: 4.445 ± 0.529
1.119GlyCys: 1.119 ± 0.204
3.948GlyAsp: 3.948 ± 0.382
4.787GlyGlu: 4.787 ± 0.422
3.077GlyPhe: 3.077 ± 0.299
4.041GlyGly: 4.041 ± 0.639
1.368GlyHis: 1.368 ± 0.207
4.756GlyIle: 4.756 ± 0.366
4.911GlyLys: 4.911 ± 0.376
5.005GlyLeu: 5.005 ± 0.347
1.772GlyMet: 1.772 ± 0.289
3.295GlyAsn: 3.295 ± 0.382
0.964GlyPro: 0.964 ± 0.176
2.331GlyGln: 2.331 ± 0.306
2.829GlyArg: 2.829 ± 0.29
4.072GlySer: 4.072 ± 0.395
4.103GlyThr: 4.103 ± 0.459
4.569GlyVal: 4.569 ± 0.451
1.088GlyTrp: 1.088 ± 0.22
2.922GlyTyr: 2.922 ± 0.337
0.0GlyXaa: 0.0 ± 0.0
His
1.368HisAla: 1.368 ± 0.234
0.373HisCys: 0.373 ± 0.117
1.43HisAsp: 1.43 ± 0.258
1.181HisGlu: 1.181 ± 0.194
0.746HisPhe: 0.746 ± 0.169
1.43HisGly: 1.43 ± 0.244
0.715HisHis: 0.715 ± 0.165
1.399HisIle: 1.399 ± 0.22
1.616HisLys: 1.616 ± 0.282
2.083HisLeu: 2.083 ± 0.28
0.435HisMet: 0.435 ± 0.156
0.933HisAsn: 0.933 ± 0.19
0.964HisPro: 0.964 ± 0.165
0.591HisGln: 0.591 ± 0.153
1.274HisArg: 1.274 ± 0.192
1.057HisSer: 1.057 ± 0.175
0.933HisThr: 0.933 ± 0.167
0.87HisVal: 0.87 ± 0.178
0.28HisTrp: 0.28 ± 0.09
0.622HisTyr: 0.622 ± 0.128
0.0HisXaa: 0.0 ± 0.0
Ile
5.129IleAla: 5.129 ± 0.307
0.653IleCys: 0.653 ± 0.168
4.818IleAsp: 4.818 ± 0.406
4.787IleGlu: 4.787 ± 0.369
2.3IlePhe: 2.3 ± 0.263
4.165IleGly: 4.165 ± 0.36
1.337IleHis: 1.337 ± 0.202
3.73IleIle: 3.73 ± 0.346
4.476IleLys: 4.476 ± 0.337
4.942IleLeu: 4.942 ± 0.398
1.772IleMet: 1.772 ± 0.27
4.134IleAsn: 4.134 ± 0.364
2.487IlePro: 2.487 ± 0.296
2.394IleGln: 2.394 ± 0.282
3.015IleArg: 3.015 ± 0.234
4.103IleSer: 4.103 ± 0.334
4.663IleThr: 4.663 ± 0.409
3.855IleVal: 3.855 ± 0.351
0.746IleTrp: 0.746 ± 0.151
2.58IleTyr: 2.58 ± 0.313
0.0IleXaa: 0.0 ± 0.0
Lys
6.466LysAla: 6.466 ± 0.515
0.622LysCys: 0.622 ± 0.173
4.911LysAsp: 4.911 ± 0.376
4.818LysGlu: 4.818 ± 0.377
3.046LysPhe: 3.046 ± 0.303
3.917LysGly: 3.917 ± 0.364
1.243LysHis: 1.243 ± 0.228
4.228LysIle: 4.228 ± 0.355
4.259LysLys: 4.259 ± 0.477
6.963LysLeu: 6.963 ± 0.591
2.362LysMet: 2.362 ± 0.263
3.171LysAsn: 3.171 ± 0.35
2.394LysPro: 2.394 ± 0.328
3.419LysGln: 3.419 ± 0.308
3.326LysArg: 3.326 ± 0.313
3.948LysSer: 3.948 ± 0.452
3.761LysThr: 3.761 ± 0.316
4.259LysVal: 4.259 ± 0.309
0.684LysTrp: 0.684 ± 0.161
3.326LysTyr: 3.326 ± 0.299
0.0LysXaa: 0.0 ± 0.0
Leu
6.808LeuAla: 6.808 ± 0.443
0.839LeuCys: 0.839 ± 0.169
6.155LeuAsp: 6.155 ± 0.417
7.274LeuGlu: 7.274 ± 0.457
2.984LeuPhe: 2.984 ± 0.316
5.626LeuGly: 5.626 ± 0.466
1.927LeuHis: 1.927 ± 0.28
4.756LeuIle: 4.756 ± 0.359
5.347LeuLys: 5.347 ± 0.368
6.186LeuLeu: 6.186 ± 0.431
2.238LeuMet: 2.238 ± 0.299
5.16LeuAsn: 5.16 ± 0.402
3.482LeuPro: 3.482 ± 0.326
3.077LeuGln: 3.077 ± 0.34
3.668LeuArg: 3.668 ± 0.267
4.725LeuSer: 4.725 ± 0.375
4.476LeuThr: 4.476 ± 0.351
5.222LeuVal: 5.222 ± 0.453
0.622LeuTrp: 0.622 ± 0.148
2.673LeuTyr: 2.673 ± 0.31
0.0LeuXaa: 0.0 ± 0.0
Met
1.834MetAla: 1.834 ± 0.231
0.435MetCys: 0.435 ± 0.108
1.43MetAsp: 1.43 ± 0.198
2.145MetGlu: 2.145 ± 0.279
0.964MetPhe: 0.964 ± 0.191
1.679MetGly: 1.679 ± 0.217
0.622MetHis: 0.622 ± 0.144
2.021MetIle: 2.021 ± 0.333
2.238MetLys: 2.238 ± 0.267
2.083MetLeu: 2.083 ± 0.316
0.528MetMet: 0.528 ± 0.128
1.119MetAsn: 1.119 ± 0.19
0.777MetPro: 0.777 ± 0.14
1.181MetGln: 1.181 ± 0.204
1.212MetArg: 1.212 ± 0.159
2.207MetSer: 2.207 ± 0.275
1.958MetThr: 1.958 ± 0.255
1.15MetVal: 1.15 ± 0.183
0.187MetTrp: 0.187 ± 0.066
1.057MetTyr: 1.057 ± 0.176
0.0MetXaa: 0.0 ± 0.0
Asn
3.45AsnAla: 3.45 ± 0.442
0.653AsnCys: 0.653 ± 0.149
2.673AsnAsp: 2.673 ± 0.266
2.767AsnGlu: 2.767 ± 0.276
1.741AsnPhe: 1.741 ± 0.219
4.072AsnGly: 4.072 ± 0.419
0.808AsnHis: 0.808 ± 0.213
3.855AsnIle: 3.855 ± 0.309
4.134AsnLys: 4.134 ± 0.379
4.694AsnLeu: 4.694 ± 0.378
1.212AsnMet: 1.212 ± 0.171
2.735AsnAsn: 2.735 ± 0.306
2.362AsnPro: 2.362 ± 0.262
1.43AsnGln: 1.43 ± 0.197
2.704AsnArg: 2.704 ± 0.276
3.513AsnSer: 3.513 ± 0.37
3.264AsnThr: 3.264 ± 0.394
3.792AsnVal: 3.792 ± 0.326
0.684AsnTrp: 0.684 ± 0.165
1.803AsnTyr: 1.803 ± 0.246
0.0AsnXaa: 0.0 ± 0.0
Pro
2.269ProAla: 2.269 ± 0.295
0.311ProCys: 0.311 ± 0.094
2.642ProAsp: 2.642 ± 0.28
3.015ProGlu: 3.015 ± 0.347
1.461ProPhe: 1.461 ± 0.209
1.71ProGly: 1.71 ± 0.227
0.746ProHis: 0.746 ± 0.155
1.958ProIle: 1.958 ± 0.296
2.487ProLys: 2.487 ± 0.307
2.083ProLeu: 2.083 ± 0.238
0.777ProMet: 0.777 ± 0.154
1.896ProAsn: 1.896 ± 0.286
1.306ProPro: 1.306 ± 0.285
1.181ProGln: 1.181 ± 0.231
1.554ProArg: 1.554 ± 0.217
2.145ProSer: 2.145 ± 0.274
1.803ProThr: 1.803 ± 0.229
2.86ProVal: 2.86 ± 0.283
0.435ProTrp: 0.435 ± 0.109
1.896ProTyr: 1.896 ± 0.231
0.0ProXaa: 0.0 ± 0.0
Gln
2.984GlnAla: 2.984 ± 0.522
0.497GlnCys: 0.497 ± 0.121
2.176GlnAsp: 2.176 ± 0.249
2.86GlnGlu: 2.86 ± 0.367
1.43GlnPhe: 1.43 ± 0.222
1.834GlnGly: 1.834 ± 0.216
0.591GlnHis: 0.591 ± 0.133
2.362GlnIle: 2.362 ± 0.27
2.86GlnLys: 2.86 ± 0.356
3.699GlnLeu: 3.699 ± 0.363
0.964GlnMet: 0.964 ± 0.171
1.679GlnAsn: 1.679 ± 0.243
0.497GlnPro: 0.497 ± 0.121
1.927GlnGln: 1.927 ± 0.336
1.803GlnArg: 1.803 ± 0.233
2.083GlnSer: 2.083 ± 0.247
2.021GlnThr: 2.021 ± 0.282
2.487GlnVal: 2.487 ± 0.278
0.684GlnTrp: 0.684 ± 0.164
1.492GlnTyr: 1.492 ± 0.207
0.0GlnXaa: 0.0 ± 0.0
Arg
3.482ArgAla: 3.482 ± 0.347
0.373ArgCys: 0.373 ± 0.121
3.357ArgAsp: 3.357 ± 0.34
3.295ArgGlu: 3.295 ± 0.245
1.741ArgPhe: 1.741 ± 0.234
3.761ArgGly: 3.761 ± 0.305
0.87ArgHis: 0.87 ± 0.206
3.388ArgIle: 3.388 ± 0.357
3.108ArgLys: 3.108 ± 0.372
3.513ArgLeu: 3.513 ± 0.372
1.679ArgMet: 1.679 ± 0.236
2.58ArgAsn: 2.58 ± 0.266
1.212ArgPro: 1.212 ± 0.201
1.772ArgGln: 1.772 ± 0.198
2.767ArgArg: 2.767 ± 0.353
2.518ArgSer: 2.518 ± 0.269
2.518ArgThr: 2.518 ± 0.216
2.829ArgVal: 2.829 ± 0.253
0.373ArgTrp: 0.373 ± 0.105
2.021ArgTyr: 2.021 ± 0.274
0.0ArgXaa: 0.0 ± 0.0
Ser
4.072SerAla: 4.072 ± 0.529
0.746SerCys: 0.746 ± 0.166
3.046SerAsp: 3.046 ± 0.251
3.544SerGlu: 3.544 ± 0.385
3.108SerPhe: 3.108 ± 0.258
4.974SerGly: 4.974 ± 0.462
0.901SerHis: 0.901 ± 0.147
4.321SerIle: 4.321 ± 0.332
4.196SerLys: 4.196 ± 0.371
5.595SerLeu: 5.595 ± 0.436
1.243SerMet: 1.243 ± 0.206
3.855SerAsn: 3.855 ± 0.331
2.083SerPro: 2.083 ± 0.256
1.772SerGln: 1.772 ± 0.281
2.891SerArg: 2.891 ± 0.282
4.103SerSer: 4.103 ± 0.379
3.575SerThr: 3.575 ± 0.342
3.544SerVal: 3.544 ± 0.366
1.212SerTrp: 1.212 ± 0.172
2.362SerTyr: 2.362 ± 0.222
0.0SerXaa: 0.0 ± 0.0
Thr
4.538ThrAla: 4.538 ± 0.546
0.56ThrCys: 0.56 ± 0.134
3.45ThrAsp: 3.45 ± 0.26
3.388ThrGlu: 3.388 ± 0.3
2.456ThrPhe: 2.456 ± 0.263
5.005ThrGly: 5.005 ± 0.444
1.088ThrHis: 1.088 ± 0.174
4.228ThrIle: 4.228 ± 0.388
3.823ThrLys: 3.823 ± 0.319
4.756ThrLeu: 4.756 ± 0.345
1.274ThrMet: 1.274 ± 0.191
3.202ThrAsn: 3.202 ± 0.328
2.518ThrPro: 2.518 ± 0.259
1.927ThrGln: 1.927 ± 0.235
2.3ThrArg: 2.3 ± 0.25
3.357ThrSer: 3.357 ± 0.331
3.264ThrThr: 3.264 ± 0.366
3.886ThrVal: 3.886 ± 0.397
0.653ThrTrp: 0.653 ± 0.183
1.989ThrTyr: 1.989 ± 0.223
0.0ThrXaa: 0.0 ± 0.0
Val
5.098ValAla: 5.098 ± 0.382
0.715ValCys: 0.715 ± 0.159
4.476ValAsp: 4.476 ± 0.419
3.761ValGlu: 3.761 ± 0.311
2.642ValPhe: 2.642 ± 0.239
3.233ValGly: 3.233 ± 0.341
1.119ValHis: 1.119 ± 0.187
4.103ValIle: 4.103 ± 0.429
4.974ValLys: 4.974 ± 0.35
4.911ValLeu: 4.911 ± 0.315
1.523ValMet: 1.523 ± 0.212
3.171ValAsn: 3.171 ± 0.363
2.518ValPro: 2.518 ± 0.28
2.083ValGln: 2.083 ± 0.322
3.046ValArg: 3.046 ± 0.347
4.259ValSer: 4.259 ± 0.372
3.886ValThr: 3.886 ± 0.346
4.601ValVal: 4.601 ± 0.447
0.715ValTrp: 0.715 ± 0.15
2.549ValTyr: 2.549 ± 0.242
0.0ValXaa: 0.0 ± 0.0
Trp
0.404TrpAla: 0.404 ± 0.115
0.187TrpCys: 0.187 ± 0.084
1.119TrpAsp: 1.119 ± 0.15
1.119TrpGlu: 1.119 ± 0.225
0.591TrpPhe: 0.591 ± 0.134
0.933TrpGly: 0.933 ± 0.222
0.155TrpHis: 0.155 ± 0.072
0.715TrpIle: 0.715 ± 0.158
0.995TrpLys: 0.995 ± 0.152
1.15TrpLeu: 1.15 ± 0.195
0.497TrpMet: 0.497 ± 0.121
0.653TrpAsn: 0.653 ± 0.149
0.466TrpPro: 0.466 ± 0.119
0.497TrpGln: 0.497 ± 0.118
0.746TrpArg: 0.746 ± 0.169
0.684TrpSer: 0.684 ± 0.199
0.653TrpThr: 0.653 ± 0.125
0.684TrpVal: 0.684 ± 0.161
0.155TrpTrp: 0.155 ± 0.066
0.373TrpTyr: 0.373 ± 0.097
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.487TyrAla: 2.487 ± 0.255
0.715TyrCys: 0.715 ± 0.143
2.767TyrAsp: 2.767 ± 0.277
2.052TyrGlu: 2.052 ± 0.297
2.114TyrPhe: 2.114 ± 0.292
2.114TyrGly: 2.114 ± 0.244
1.181TyrHis: 1.181 ± 0.184
2.331TyrIle: 2.331 ± 0.26
2.549TyrLys: 2.549 ± 0.264
2.891TyrLeu: 2.891 ± 0.264
1.212TyrMet: 1.212 ± 0.198
2.456TyrAsn: 2.456 ± 0.235
1.461TyrPro: 1.461 ± 0.209
1.834TyrGln: 1.834 ± 0.243
1.927TyrArg: 1.927 ± 0.258
2.704TyrSer: 2.704 ± 0.226
2.829TyrThr: 2.829 ± 0.283
2.083TyrVal: 2.083 ± 0.271
0.497TyrTrp: 0.497 ± 0.103
1.461TyrTyr: 1.461 ± 0.206
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 156 proteins (32171 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski