Opisthorchis felineus

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Spiralia; Lophotrochozoa; Platyhelminthes; Trematoda; Digenea; Opisthorchiida; Opisthorchiata; Opisthorchiidae; Opisthorchis

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18330 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4S2MAA0|A0A4S2MAA0_OPIFE Glucosamine 6-phosphate N-acetyltransferase OS=Opisthorchis felineus OX=147828 GN=CRM22_001513 PE=3 SV=1
MM1 pKa = 6.95MVGFIMLIATALLSNVLAEE20 pKa = 5.52DD21 pKa = 3.27LMEE24 pKa = 4.83YY25 pKa = 9.85DD26 pKa = 3.76SCMAACEE33 pKa = 4.17EE34 pKa = 4.55KK35 pKa = 10.72FAGDD39 pKa = 3.31TDD41 pKa = 3.57NDD43 pKa = 3.53QFEE46 pKa = 4.59VCKK49 pKa = 10.67DD50 pKa = 3.42SCNEE54 pKa = 3.92DD55 pKa = 3.27QKK57 pKa = 11.45DD58 pKa = 3.43RR59 pKa = 11.84CVAADD64 pKa = 3.64PQAEE68 pKa = 4.35EE69 pKa = 4.31TCLKK73 pKa = 10.75AGEE76 pKa = 4.15QRR78 pKa = 11.84CKK80 pKa = 10.78DD81 pKa = 3.4RR82 pKa = 11.84CEE84 pKa = 4.16EE85 pKa = 4.53DD86 pKa = 4.05SDD88 pKa = 4.39GCPLLCEE95 pKa = 4.33ALFSKK100 pKa = 10.73DD101 pKa = 3.98DD102 pKa = 3.69EE103 pKa = 4.52

Molecular weight: 11.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4S2JUJ9|A0A4S2JUJ9_OPIFE BZIP domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_011255 PE=4 SV=1
MM1 pKa = 7.66GSHH4 pKa = 5.78KK5 pKa = 8.38TLRR8 pKa = 11.84MKK10 pKa = 10.41TKK12 pKa = 10.36LGKK15 pKa = 9.54KK16 pKa = 8.85QKK18 pKa = 9.03QNRR21 pKa = 11.84TLPHH25 pKa = 5.31WTRR28 pKa = 11.84LRR30 pKa = 11.84TGNKK34 pKa = 8.34IRR36 pKa = 11.84YY37 pKa = 5.83NAKK40 pKa = 8.24RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.1WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.89LKK50 pKa = 10.55LL51 pKa = 3.39

Molecular weight: 6.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11402

6928

18330

10710057

36

18669

584.3

65.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.832 ± 0.017

2.115 ± 0.012

5.204 ± 0.013

5.91 ± 0.021

3.725 ± 0.01

5.525 ± 0.018

2.759 ± 0.008

4.608 ± 0.012

4.424 ± 0.017

9.937 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.027 ± 0.006

4.098 ± 0.012

6.025 ± 0.021

4.395 ± 0.011

6.439 ± 0.015

9.416 ± 0.023

6.38 ± 0.012

6.381 ± 0.014

1.142 ± 0.005

2.66 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski