uncultured phage_MedDCM-OCT-S30-C28

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Fussvirus; Fussvirus S30C28

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6S4PDV3|A0A6S4PDV3_9CAUD Uncharacterized protein OS=uncultured phage_MedDCM-OCT-S30-C28 OX=2741076 PE=4 SV=1
MM1 pKa = 7.74SYY3 pKa = 10.43LAQVSYY9 pKa = 10.36TGDD12 pKa = 3.32GSTTQYY18 pKa = 11.16SITFPFLDD26 pKa = 3.73STHH29 pKa = 5.29VKK31 pKa = 10.74AFIGGVATTAFTISSSTLTFTSAPANSAVIRR62 pKa = 11.84IEE64 pKa = 3.86RR65 pKa = 11.84QTPTSSRR72 pKa = 11.84LVDD75 pKa = 4.11FTDD78 pKa = 4.41GSVLTEE84 pKa = 3.91SDD86 pKa = 3.6LDD88 pKa = 3.76RR89 pKa = 11.84SADD92 pKa = 3.47QNFYY96 pKa = 10.3IAQEE100 pKa = 3.83ISDD103 pKa = 4.29ANQSAMTIGTDD114 pKa = 4.3DD115 pKa = 4.6KK116 pKa = 11.5FDD118 pKa = 3.85AQSKK122 pKa = 9.01IIKK125 pKa = 9.87NVANPVDD132 pKa = 3.74NQDD135 pKa = 3.81AVTKK139 pKa = 10.51YY140 pKa = 10.39YY141 pKa = 11.04LEE143 pKa = 4.28NTWLSSANKK152 pKa = 8.63TALTTVNSNITAITNVNSNSTNINAVNSNSTNINLVAGSIGSVNTVATDD201 pKa = 3.31IAKK204 pKa = 10.49VIAVANDD211 pKa = 3.2LAEE214 pKa = 4.27AVSEE218 pKa = 4.53VEE220 pKa = 4.57TVANDD225 pKa = 3.74LNEE228 pKa = 4.33STSEE232 pKa = 3.68IDD234 pKa = 3.35TVANAITNVDD244 pKa = 3.0IVGNAIANVNTLAGVSNLQNLANAHH269 pKa = 5.0SQIVTVGNNIPAVEE283 pKa = 4.14AFGNTYY289 pKa = 9.96KK290 pKa = 10.42ISASAPSSPIEE301 pKa = 3.8GTLWFDD307 pKa = 3.32TTADD311 pKa = 3.03IMKK314 pKa = 9.86VYY316 pKa = 10.5DD317 pKa = 3.6GSSFVNAGSSVNGTSGRR334 pKa = 11.84FKK336 pKa = 11.11YY337 pKa = 10.11IATAGQTTFTGASHH351 pKa = 7.24SDD353 pKa = 3.16TGSNTLTYY361 pKa = 10.48DD362 pKa = 2.98AGFIDD367 pKa = 4.02VFKK370 pKa = 11.19NGVHH374 pKa = 7.05LDD376 pKa = 3.67PSDD379 pKa = 3.64YY380 pKa = 10.74TATDD384 pKa = 3.55GNNLVLDD391 pKa = 4.39VACSLNDD398 pKa = 3.62EE399 pKa = 4.53IYY401 pKa = 10.33ILTFGTFSLASISASAITSNTLGVARR427 pKa = 11.84GGTGKK432 pKa = 8.18TVSDD436 pKa = 4.53LSGQAGKK443 pKa = 10.68VLTVNASQNGYY454 pKa = 9.99DD455 pKa = 3.71LASASSAEE463 pKa = 4.25VYY465 pKa = 10.72GFEE468 pKa = 4.55QYY470 pKa = 11.33YY471 pKa = 10.61NPSTLIKK478 pKa = 9.33TVTVVGGKK486 pKa = 8.88FVIDD490 pKa = 4.03GVSQDD495 pKa = 3.43TLEE498 pKa = 4.46LYY500 pKa = 10.19EE501 pKa = 4.37GNTYY505 pKa = 9.74VFNYY509 pKa = 9.27PSSHH513 pKa = 6.56PFKK516 pKa = 10.86FSTTSDD522 pKa = 3.38GTHH525 pKa = 6.58GGGSEE530 pKa = 4.15YY531 pKa = 8.44TTGVTHH537 pKa = 7.27NSSTQVTIVVASNAPTLYY555 pKa = 10.12YY556 pKa = 10.77YY557 pKa = 10.4CASHH561 pKa = 6.75SAMGGQANTPVPADD575 pKa = 3.18NHH577 pKa = 5.92LRR579 pKa = 11.84IITTNQGADD588 pKa = 3.66NISSSQYY595 pKa = 11.63ANFDD599 pKa = 3.4DD600 pKa = 5.36SMFSASGFSFAITNGEE616 pKa = 4.32LIATII621 pKa = 4.25

Molecular weight: 64.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6S4PDT1|A0A6S4PDT1_9CAUD Uncharacterized protein OS=uncultured phage_MedDCM-OCT-S30-C28 OX=2741076 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.47GAINSLNNRR11 pKa = 11.84GAMVEE16 pKa = 4.3LVDD19 pKa = 4.32TPVLGTGLLRR29 pKa = 11.84GEE31 pKa = 4.55GSSPFRR37 pKa = 11.84PTKK40 pKa = 10.39DD41 pKa = 4.11DD42 pKa = 3.54YY43 pKa = 11.99DD44 pKa = 3.67GVYY47 pKa = 10.32DD48 pKa = 3.95ISYY51 pKa = 10.75SSTIEE56 pKa = 3.9KK57 pKa = 10.54KK58 pKa = 10.65KK59 pKa = 10.87GVVNNKK65 pKa = 9.37KK66 pKa = 10.85GNFGSLRR73 pKa = 11.84KK74 pKa = 9.58NHH76 pKa = 5.88TFHH79 pKa = 7.53LNNTSANNSLSTRR92 pKa = 11.84CFLKK96 pKa = 10.82LISSTPVWWSSRR108 pKa = 11.84RR109 pKa = 11.84FTISTRR115 pKa = 11.84RR116 pKa = 11.84RR117 pKa = 11.84TPVLVSTKK125 pKa = 10.47EE126 pKa = 3.9MFSQCPQKK134 pKa = 10.57LIKK137 pKa = 9.98HH138 pKa = 6.25HH139 pKa = 6.0YY140 pKa = 9.45SYY142 pKa = 11.9NN143 pKa = 3.43

Molecular weight: 16.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

11730

40

1231

230.0

25.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.556 ± 0.384

0.878 ± 0.161

6.01 ± 0.248

6.428 ± 0.495

4.263 ± 0.314

6.044 ± 0.413

1.628 ± 0.237

6.667 ± 0.274

8.986 ± 0.639

7.945 ± 0.363

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.114 ± 0.186

6.394 ± 0.282

3.214 ± 0.258

4.211 ± 0.24

4.177 ± 0.279

7.238 ± 0.536

6.726 ± 0.541

5.968 ± 0.317

0.929 ± 0.143

3.623 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski