Pseudogymnoascus sp. VKM F-4516 (FW-969)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus; unclassified Pseudogymnoascus

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9362 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A094DBN8|A0A094DBN8_9PEZI TBPIP domain-containing protein OS=Pseudogymnoascus sp. VKM F-4516 (FW-969) OX=1420910 GN=V497_02805 PE=3 SV=1
MM1 pKa = 7.54SGYY4 pKa = 11.32GNDD7 pKa = 4.3NNDD10 pKa = 3.09SSNTRR15 pKa = 11.84GGDD18 pKa = 3.31SYY20 pKa = 12.02GSGNTGSGNNDD31 pKa = 3.02SYY33 pKa = 11.79GSSGNTGSDD42 pKa = 3.44SYY44 pKa = 11.59GSSGNTRR51 pKa = 11.84SSGNDD56 pKa = 3.14DD57 pKa = 4.01SYY59 pKa = 11.97GSSNTRR65 pKa = 11.84SSDD68 pKa = 3.38NNDD71 pKa = 3.1SYY73 pKa = 11.93GSSNTRR79 pKa = 11.84SSDD82 pKa = 3.38NNDD85 pKa = 3.1SYY87 pKa = 11.93GSSNTRR93 pKa = 11.84SSDD96 pKa = 3.38NNDD99 pKa = 3.16SYY101 pKa = 11.89GSSGNSRR108 pKa = 11.84SNDD111 pKa = 3.05NDD113 pKa = 3.82SYY115 pKa = 11.64GSSNKK120 pKa = 9.81SSSDD124 pKa = 3.34NNDD127 pKa = 2.94SYY129 pKa = 11.93GSSNTRR135 pKa = 11.84SGDD138 pKa = 3.24NDD140 pKa = 4.0SYY142 pKa = 11.66GSSGNSRR149 pKa = 11.84SDD151 pKa = 3.38NNDD154 pKa = 2.75SYY156 pKa = 11.99GSSNKK161 pKa = 9.78SSSGNNDD168 pKa = 2.79SYY170 pKa = 11.87GSSNNDD176 pKa = 2.76SYY178 pKa = 11.96GSSNTRR184 pKa = 11.84SSGNDD189 pKa = 3.14DD190 pKa = 4.32SYY192 pKa = 12.16GSSNNNNSSDD202 pKa = 3.85SYY204 pKa = 11.03GSSGGKK210 pKa = 7.85TGNSTVDD217 pKa = 3.35KK218 pKa = 10.63LVDD221 pKa = 3.39KK222 pKa = 11.21ASDD225 pKa = 3.61FLGSNNSSSGNNNSNYY241 pKa = 10.73

Molecular weight: 24.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A094E0U6|A0A094E0U6_9PEZI Arf-GAP domain-containing protein (Fragment) OS=Pseudogymnoascus sp. VKM F-4516 (FW-969) OX=1420910 GN=V497_01425 PE=4 SV=1
MM1 pKa = 6.8ITQRR5 pKa = 11.84QATASLCRR13 pKa = 11.84KK14 pKa = 9.33RR15 pKa = 11.84NTVFVTLRR23 pKa = 11.84LRR25 pKa = 11.84VKK27 pKa = 10.4SPIRR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84LGEE37 pKa = 4.03SFGDD41 pKa = 3.74TVGGGGHH48 pKa = 5.88EE49 pKa = 4.13RR50 pKa = 11.84WAGGPGRR57 pKa = 11.84CSQGVLGRR65 pKa = 11.84ARR67 pKa = 11.84RR68 pKa = 11.84FGRR71 pKa = 11.84GRR73 pKa = 11.84WGWGRR78 pKa = 11.84LEE80 pKa = 3.97MGTWEE85 pKa = 4.5SFPGVTPGGFGVGFGLLVRR104 pKa = 11.84FDD106 pKa = 4.55GSFLCLDD113 pKa = 4.32GGRR116 pKa = 11.84EE117 pKa = 4.16GGAGEE122 pKa = 4.38SGNVAGILLVRR133 pKa = 11.84KK134 pKa = 9.68VKK136 pKa = 10.66LGQRR140 pKa = 11.84DD141 pKa = 2.76AWIPHH146 pKa = 5.56TLPVGRR152 pKa = 11.84RR153 pKa = 11.84DD154 pKa = 3.89ADD156 pKa = 3.54EE157 pKa = 5.72LSDD160 pKa = 3.65KK161 pKa = 10.5RR162 pKa = 11.84CPIGGDD168 pKa = 2.99IWCGGINAVPIKK180 pKa = 8.33TAVMRR185 pKa = 11.84APDD188 pKa = 3.67LAYY191 pKa = 10.4VFRR194 pKa = 11.84PAVTSMPVHH203 pKa = 5.17QLKK206 pKa = 10.31PRR208 pKa = 11.84GIAVTPGNFFQALSVDD224 pKa = 3.88MEE226 pKa = 4.36HH227 pKa = 7.22AGNSVYY233 pKa = 10.74DD234 pKa = 4.14LRR236 pKa = 11.84GQPTWTSSVTEE247 pKa = 4.23NVDD250 pKa = 2.87CDD252 pKa = 3.66MPNFSTPRR260 pKa = 11.84TSFNN264 pKa = 3.31

Molecular weight: 28.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9362

0

9362

4879565

8

5496

521.2

57.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.016 ± 0.027

1.171 ± 0.01

5.634 ± 0.015

6.304 ± 0.024

3.608 ± 0.015

7.266 ± 0.026

2.224 ± 0.01

5.0 ± 0.018

5.042 ± 0.022

8.67 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.137 ± 0.01

3.735 ± 0.014

6.01 ± 0.025

3.854 ± 0.019

5.819 ± 0.02

8.141 ± 0.025

6.071 ± 0.018

6.131 ± 0.019

1.406 ± 0.008

2.763 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski