Amino acid dipepetide frequency for Pseudogymnoascus sp. VKM F-4516 (FW-969)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.151AlaAla: 10.151 ± 0.078	1.041AlaCys: 1.041 ± 0.017	4.447AlaAsp: 4.447 ± 0.035	5.5AlaGlu: 5.5 ± 0.053	3.107AlaPhe: 3.107 ± 0.027	6.326AlaGly: 6.326 ± 0.042	1.758AlaHis: 1.758 ± 0.019	4.524AlaIle: 4.524 ± 0.035	4.564AlaLys: 4.564 ± 0.043	7.895AlaLeu: 7.895 ± 0.05	2.004AlaMet: 2.004 ± 0.019	3.136AlaAsn: 3.136 ± 0.03	5.402AlaPro: 5.402 ± 0.06	3.472AlaGln: 3.472 ± 0.035	4.859AlaArg: 4.859 ± 0.04	7.363AlaSer: 7.363 ± 0.044	5.664AlaThr: 5.664 ± 0.04	5.581AlaVal: 5.581 ± 0.038	1.144AlaTrp: 1.144 ± 0.016	2.222AlaTyr: 2.222 ± 0.021	0.0AlaXaa: 0.0 ± 0.0
Cys	0.955CysAla: 0.955 ± 0.016	0.245CysCys: 0.245 ± 0.009	0.639CysAsp: 0.639 ± 0.013	0.587CysGlu: 0.587 ± 0.013	0.493CysPhe: 0.493 ± 0.01	0.968CysGly: 0.968 ± 0.015	0.305CysHis: 0.305 ± 0.008	0.673CysIle: 0.673 ± 0.012	0.504CysLys: 0.504 ± 0.012	1.137CysLeu: 1.137 ± 0.016	0.263CysMet: 0.263 ± 0.007	0.42CysAsn: 0.42 ± 0.01	0.606CysPro: 0.606 ± 0.015	0.399CysGln: 0.399 ± 0.008	0.668CysArg: 0.668 ± 0.012	0.862CysSer: 0.862 ± 0.015	0.696CysThr: 0.696 ± 0.015	0.746CysVal: 0.746 ± 0.012	0.189CysTrp: 0.189 ± 0.006	0.354CysTyr: 0.354 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	4.926AspAla: 4.926 ± 0.036	0.587AspCys: 0.587 ± 0.011	4.229AspAsp: 4.229 ± 0.047	4.524AspGlu: 4.524 ± 0.044	2.199AspPhe: 2.199 ± 0.024	4.223AspGly: 4.223 ± 0.026	1.094AspHis: 1.094 ± 0.014	3.172AspIle: 3.172 ± 0.03	2.459AspLys: 2.459 ± 0.024	4.842AspLeu: 4.842 ± 0.034	1.25AspMet: 1.25 ± 0.019	2.003AspAsn: 2.003 ± 0.024	3.06AspPro: 3.06 ± 0.026	1.702AspGln: 1.702 ± 0.019	2.831AspArg: 2.831 ± 0.027	4.119AspSer: 4.119 ± 0.035	3.009AspThr: 3.009 ± 0.024	3.669AspVal: 3.669 ± 0.03	0.836AspTrp: 0.836 ± 0.014	1.603AspTyr: 1.603 ± 0.019	0.0AspXaa: 0.0 ± 0.0
Glu	5.847GluAla: 5.847 ± 0.052	0.61GluCys: 0.61 ± 0.011	4.148GluAsp: 4.148 ± 0.038	5.853GluGlu: 5.853 ± 0.063	1.934GluPhe: 1.934 ± 0.022	4.296GluGly: 4.296 ± 0.034	1.264GluHis: 1.264 ± 0.015	3.128GluIle: 3.128 ± 0.03	3.841GluLys: 3.841 ± 0.039	5.137GluLeu: 5.137 ± 0.038	1.503GluMet: 1.503 ± 0.018	2.262GluAsn: 2.262 ± 0.023	2.731GluPro: 2.731 ± 0.03	2.317GluGln: 2.317 ± 0.024	3.991GluArg: 3.991 ± 0.039	4.308GluSer: 4.308 ± 0.036	3.44GluThr: 3.44 ± 0.027	3.845GluVal: 3.845 ± 0.033	0.885GluTrp: 0.885 ± 0.014	1.698GluTyr: 1.698 ± 0.018	0.0GluXaa: 0.0 ± 0.0
Phe	3.077PheAla: 3.077 ± 0.027	0.527PheCys: 0.527 ± 0.011	2.205PheAsp: 2.205 ± 0.023	2.145PheGlu: 2.145 ± 0.021	1.516PhePhe: 1.516 ± 0.023	2.926PheGly: 2.926 ± 0.03	0.821PheHis: 0.821 ± 0.012	1.834PheIle: 1.834 ± 0.024	1.583PheLys: 1.583 ± 0.02	3.196PheLeu: 3.196 ± 0.029	0.77PheMet: 0.77 ± 0.014	1.441PheAsn: 1.441 ± 0.018	1.865PhePro: 1.865 ± 0.02	1.335PheGln: 1.335 ± 0.017	1.774PheArg: 1.774 ± 0.022	2.858PheSer: 2.858 ± 0.026	2.145PheThr: 2.145 ± 0.025	2.347PheVal: 2.347 ± 0.025	0.61PheTrp: 0.61 ± 0.012	1.105PheTyr: 1.105 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	6.065GlyAla: 6.065 ± 0.046	0.893GlyCys: 0.893 ± 0.015	3.874GlyAsp: 3.874 ± 0.029	4.158GlyGlu: 4.158 ± 0.033	2.84GlyPhe: 2.84 ± 0.029	6.983GlyGly: 6.983 ± 0.076	1.619GlyHis: 1.619 ± 0.021	3.646GlyIle: 3.646 ± 0.028	3.824GlyLys: 3.824 ± 0.034	6.074GlyLeu: 6.074 ± 0.043	1.692GlyMet: 1.692 ± 0.021	2.736GlyAsn: 2.736 ± 0.029	3.368GlyPro: 3.368 ± 0.032	2.601GlyGln: 2.601 ± 0.03	4.219GlyArg: 4.219 ± 0.04	5.867GlySer: 5.867 ± 0.046	4.133GlyThr: 4.133 ± 0.034	4.702GlyVal: 4.702 ± 0.035	1.202GlyTrp: 1.202 ± 0.018	2.167GlyTyr: 2.167 ± 0.026	0.0GlyXaa: 0.0 ± 0.0
His	1.748HisAla: 1.748 ± 0.021	0.289HisCys: 0.289 ± 0.008	1.202HisAsp: 1.202 ± 0.016	1.221HisGlu: 1.221 ± 0.016	0.867HisPhe: 0.867 ± 0.014	1.657HisGly: 1.657 ± 0.02	0.743HisHis: 0.743 ± 0.015	1.209HisIle: 1.209 ± 0.015	0.918HisLys: 0.918 ± 0.014	2.069HisLeu: 2.069 ± 0.02	0.444HisMet: 0.444 ± 0.011	0.839HisAsn: 0.839 ± 0.012	1.554HisPro: 1.554 ± 0.019	0.892HisGln: 0.892 ± 0.016	1.352HisArg: 1.352 ± 0.018	1.707HisSer: 1.707 ± 0.022	1.244HisThr: 1.244 ± 0.016	1.32HisVal: 1.32 ± 0.016	0.303HisTrp: 0.303 ± 0.009	0.664HisTyr: 0.664 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	4.419IleAla: 4.419 ± 0.039	0.733IleCys: 0.733 ± 0.013	2.919IleAsp: 2.919 ± 0.027	2.964IleGlu: 2.964 ± 0.025	1.995IlePhe: 1.995 ± 0.025	3.315IleGly: 3.315 ± 0.032	1.131IleHis: 1.131 ± 0.018	2.676IleIle: 2.676 ± 0.031	2.357IleLys: 2.357 ± 0.022	4.5IleLeu: 4.5 ± 0.034	1.055IleMet: 1.055 ± 0.015	1.909IleAsn: 1.909 ± 0.023	3.073IlePro: 3.073 ± 0.032	1.871IleGln: 1.871 ± 0.02	2.665IleArg: 2.665 ± 0.025	3.991IleSer: 3.991 ± 0.036	3.064IleThr: 3.064 ± 0.026	3.19IleVal: 3.19 ± 0.028	0.711IleTrp: 0.711 ± 0.014	1.461IleTyr: 1.461 ± 0.019	0.0IleXaa: 0.0 ± 0.0
Lys	4.669LysAla: 4.669 ± 0.044	0.506LysCys: 0.506 ± 0.012	2.859LysAsp: 2.859 ± 0.029	3.684LysGlu: 3.684 ± 0.039	1.543LysPhe: 1.543 ± 0.02	3.336LysGly: 3.336 ± 0.032	1.083LysHis: 1.083 ± 0.015	2.381LysIle: 2.381 ± 0.023	3.523LysLys: 3.523 ± 0.048	4.198LysLeu: 4.198 ± 0.034	1.057LysMet: 1.057 ± 0.015	1.824LysAsn: 1.824 ± 0.023	2.796LysPro: 2.796 ± 0.03	1.777LysGln: 1.777 ± 0.021	3.377LysArg: 3.377 ± 0.036	3.752LysSer: 3.752 ± 0.032	2.894LysThr: 2.894 ± 0.029	3.035LysVal: 3.035 ± 0.03	0.697LysTrp: 0.697 ± 0.013	1.423LysTyr: 1.423 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	7.851LeuAla: 7.851 ± 0.046	1.11LeuCys: 1.11 ± 0.015	4.951LeuAsp: 4.951 ± 0.032	5.389LeuGlu: 5.389 ± 0.039	3.157LeuPhe: 3.157 ± 0.03	5.978LeuGly: 5.978 ± 0.036	2.067LeuHis: 2.067 ± 0.022	3.958LeuIle: 3.958 ± 0.039	4.302LeuLys: 4.302 ± 0.033	8.169LeuLeu: 8.169 ± 0.06	1.696LeuMet: 1.696 ± 0.022	3.087LeuAsn: 3.087 ± 0.026	5.408LeuPro: 5.408 ± 0.036	3.606LeuGln: 3.606 ± 0.03	5.349LeuArg: 5.349 ± 0.039	7.196LeuSer: 7.196 ± 0.042	4.782LeuThr: 4.782 ± 0.03	5.183LeuVal: 5.183 ± 0.037	1.152LeuTrp: 1.152 ± 0.018	2.315LeuTyr: 2.315 ± 0.024	0.0LeuXaa: 0.0 ± 0.0
Met	2.217MetAla: 2.217 ± 0.022	0.217MetCys: 0.217 ± 0.008	1.234MetAsp: 1.234 ± 0.017	1.317MetGlu: 1.317 ± 0.015	0.737MetPhe: 0.737 ± 0.013	1.567MetGly: 1.567 ± 0.025	0.48MetHis: 0.48 ± 0.01	0.961MetIle: 0.961 ± 0.014	1.057MetLys: 1.057 ± 0.014	1.78MetLeu: 1.78 ± 0.02	0.568MetMet: 0.568 ± 0.013	0.78MetAsn: 0.78 ± 0.014	1.246MetPro: 1.246 ± 0.019	0.824MetGln: 0.824 ± 0.014	1.243MetArg: 1.243 ± 0.02	1.825MetSer: 1.825 ± 0.02	1.213MetThr: 1.213 ± 0.014	1.329MetVal: 1.329 ± 0.015	0.254MetTrp: 0.254 ± 0.008	0.516MetTyr: 0.516 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	3.25AsnAla: 3.25 ± 0.03	0.454AsnCys: 0.454 ± 0.011	1.987AsnAsp: 1.987 ± 0.02	2.041AsnGlu: 2.041 ± 0.02	1.402AsnPhe: 1.402 ± 0.017	3.188AsnGly: 3.188 ± 0.032	0.807AsnHis: 0.807 ± 0.014	2.177AsnIle: 2.177 ± 0.022	1.611AsnLys: 1.611 ± 0.02	3.194AsnLeu: 3.194 ± 0.025	0.852AsnMet: 0.852 ± 0.013	1.596AsnAsn: 1.596 ± 0.019	2.414AsnPro: 2.414 ± 0.023	1.302AsnGln: 1.302 ± 0.019	1.902AsnArg: 1.902 ± 0.019	2.943AsnSer: 2.943 ± 0.027	2.276AsnThr: 2.276 ± 0.026	2.287AsnVal: 2.287 ± 0.02	0.547AsnTrp: 0.547 ± 0.011	1.116AsnTyr: 1.116 ± 0.017	0.0AsnXaa: 0.0 ± 0.0
Pro	5.494ProAla: 5.494 ± 0.055	0.484ProCys: 0.484 ± 0.01	2.972ProAsp: 2.972 ± 0.027	3.675ProGlu: 3.675 ± 0.032	1.925ProPhe: 1.925 ± 0.022	3.94ProGly: 3.94 ± 0.039	1.271ProHis: 1.271 ± 0.019	2.613ProIle: 2.613 ± 0.025	2.837ProLys: 2.837 ± 0.032	4.745ProLeu: 4.745 ± 0.035	1.022ProMet: 1.022 ± 0.016	2.233ProAsn: 2.233 ± 0.024	5.176ProPro: 5.176 ± 0.074	2.452ProGln: 2.452 ± 0.036	3.336ProArg: 3.336 ± 0.035	5.843ProSer: 5.843 ± 0.058	4.312ProThr: 4.312 ± 0.042	3.557ProVal: 3.557 ± 0.029	0.702ProTrp: 0.702 ± 0.013	1.506ProTyr: 1.506 ± 0.021	0.0ProXaa: 0.0 ± 0.0
Gln	3.383GlnAla: 3.383 ± 0.031	0.413GlnCys: 0.413 ± 0.01	1.951GlnAsp: 1.951 ± 0.022	2.232GlnGlu: 2.232 ± 0.021	1.256GlnPhe: 1.256 ± 0.016	2.507GlnGly: 2.507 ± 0.025	0.958GlnHis: 0.958 ± 0.017	1.841GlnIle: 1.841 ± 0.018	1.938GlnLys: 1.938 ± 0.023	3.301GlnLeu: 3.301 ± 0.025	0.847GlnMet: 0.847 ± 0.014	1.537GlnAsn: 1.537 ± 0.021	2.429GlnPro: 2.429 ± 0.034	2.514GlnGln: 2.514 ± 0.063	2.476GlnArg: 2.476 ± 0.028	2.961GlnSer: 2.961 ± 0.03	2.244GlnThr: 2.244 ± 0.023	2.026GlnVal: 2.026 ± 0.022	0.546GlnTrp: 0.546 ± 0.011	1.174GlnTyr: 1.174 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	4.766ArgAla: 4.766 ± 0.034	0.66ArgCys: 0.66 ± 0.014	3.246ArgAsp: 3.246 ± 0.03	3.841ArgGlu: 3.841 ± 0.039	1.938ArgPhe: 1.938 ± 0.021	3.902ArgGly: 3.902 ± 0.038	1.429ArgHis: 1.429 ± 0.018	2.781ArgIle: 2.781 ± 0.027	3.368ArgLys: 3.368 ± 0.031	4.99ArgLeu: 4.99 ± 0.035	1.227ArgMet: 1.227 ± 0.016	2.209ArgAsn: 2.209 ± 0.023	3.284ArgPro: 3.284 ± 0.037	2.454ArgGln: 2.454 ± 0.025	4.838ArgArg: 4.838 ± 0.051	4.487ArgSer: 4.487 ± 0.043	3.162ArgThr: 3.162 ± 0.03	3.267ArgVal: 3.267 ± 0.028	0.838ArgTrp: 0.838 ± 0.013	1.509ArgTyr: 1.509 ± 0.017	0.0ArgXaa: 0.0 ± 0.0
Ser	6.847SerAla: 6.847 ± 0.041	0.858SerCys: 0.858 ± 0.016	4.239SerAsp: 4.239 ± 0.038	4.145SerGlu: 4.145 ± 0.032	2.931SerPhe: 2.931 ± 0.026	5.771SerGly: 5.771 ± 0.045	1.847SerHis: 1.847 ± 0.024	4.094SerIle: 4.094 ± 0.028	3.906SerLys: 3.906 ± 0.033	7.009SerLeu: 7.009 ± 0.044	1.636SerMet: 1.636 ± 0.02	3.096SerAsn: 3.096 ± 0.028	5.536SerPro: 5.536 ± 0.065	3.141SerGln: 3.141 ± 0.032	4.681SerArg: 4.681 ± 0.042	8.321SerSer: 8.321 ± 0.078	5.666SerThr: 5.666 ± 0.042	4.508SerVal: 4.508 ± 0.032	1.062SerTrp: 1.062 ± 0.015	2.113SerTyr: 2.113 ± 0.023	0.0SerXaa: 0.0 ± 0.0
Thr	5.496ThrAla: 5.496 ± 0.037	0.716ThrCys: 0.716 ± 0.011	2.915ThrAsp: 2.915 ± 0.029	3.232ThrGlu: 3.232 ± 0.032	2.203ThrPhe: 2.203 ± 0.023	4.327ThrGly: 4.327 ± 0.033	1.269ThrHis: 1.269 ± 0.016	3.199ThrIle: 3.199 ± 0.029	2.785ThrLys: 2.785 ± 0.024	5.217ThrLeu: 5.217 ± 0.032	1.212ThrMet: 1.212 ± 0.013	2.201ThrAsn: 2.201 ± 0.025	4.58ThrPro: 4.58 ± 0.044	2.074ThrGln: 2.074 ± 0.024	3.005ThrArg: 3.005 ± 0.024	5.414ThrSer: 5.414 ± 0.048	4.671ThrThr: 4.671 ± 0.052	3.701ThrVal: 3.701 ± 0.032	0.841ThrTrp: 0.841 ± 0.015	1.653ThrTyr: 1.653 ± 0.02	0.0ThrXaa: 0.0 ± 0.0
Val	5.659ValAla: 5.659 ± 0.039	0.769ValCys: 0.769 ± 0.012	3.78ValAsp: 3.78 ± 0.029	4.111ValGlu: 4.111 ± 0.039	2.344ValPhe: 2.344 ± 0.024	4.32ValGly: 4.32 ± 0.038	1.257ValHis: 1.257 ± 0.018	2.961ValIle: 2.961 ± 0.029	3.079ValLys: 3.079 ± 0.028	5.463ValLeu: 5.463 ± 0.037	1.325ValMet: 1.325 ± 0.018	2.242ValAsn: 2.242 ± 0.022	3.509ValPro: 3.509 ± 0.029	2.217ValGln: 2.217 ± 0.021	3.247ValArg: 3.247 ± 0.027	4.459ValSer: 4.459 ± 0.033	3.517ValThr: 3.517 ± 0.032	4.512ValVal: 4.512 ± 0.042	0.849ValTrp: 0.849 ± 0.013	1.685ValTyr: 1.685 ± 0.02	0.0ValXaa: 0.0 ± 0.0
Trp	1.153TrpAla: 1.153 ± 0.017	0.187TrpCys: 0.187 ± 0.007	0.883TrpAsp: 0.883 ± 0.018	0.856TrpGlu: 0.856 ± 0.013	0.508TrpPhe: 0.508 ± 0.012	0.986TrpGly: 0.986 ± 0.017	0.327TrpHis: 0.327 ± 0.009	0.722TrpIle: 0.722 ± 0.013	0.768TrpLys: 0.768 ± 0.013	1.238TrpLeu: 1.238 ± 0.02	0.349TrpMet: 0.349 ± 0.008	0.599TrpAsn: 0.599 ± 0.012	0.581TrpPro: 0.581 ± 0.013	0.523TrpGln: 0.523 ± 0.009	0.875TrpArg: 0.875 ± 0.016	1.019TrpSer: 1.019 ± 0.017	0.895TrpThr: 0.895 ± 0.014	0.906TrpVal: 0.906 ± 0.013	0.272TrpTrp: 0.272 ± 0.009	0.409TrpTyr: 0.409 ± 0.01	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.188TyrAla: 2.188 ± 0.022	0.409TyrCys: 0.409 ± 0.01	1.66TyrAsp: 1.66 ± 0.022	1.561TyrGlu: 1.561 ± 0.018	1.186TyrPhe: 1.186 ± 0.016	2.148TyrGly: 2.148 ± 0.025	0.712TyrHis: 0.712 ± 0.012	1.446TyrIle: 1.446 ± 0.018	1.193TyrLys: 1.193 ± 0.016	2.55TyrLeu: 2.55 ± 0.025	0.593TyrMet: 0.593 ± 0.011	1.197TyrAsn: 1.197 ± 0.015	1.478TyrPro: 1.478 ± 0.02	1.061TyrGln: 1.061 ± 0.018	1.509TyrArg: 1.509 ± 0.017	2.112TyrSer: 2.112 ± 0.023	1.682TyrThr: 1.682 ± 0.021	1.592TyrVal: 1.592 ± 0.021	0.416TyrTrp: 0.416 ± 0.01	0.941TyrTyr: 0.941 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 9362 proteins (4879565 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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