Corchorus capsularis (Jute)
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 29098 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1R3HFU7|A0A1R3HFU7_COCAP J domain-containing protein OS=Corchorus capsularis OX=210143 GN=CCACVL1_19623 PE=3 SV=1
MM1 pKa = 8.28 DD2 pKa = 6.74 DD3 pKa = 5.04 YY4 pKa = 11.68 LDD6 pKa = 3.43 QCFTSSSWSDD16 pKa = 3.19 MNVKK20 pKa = 9.42 EE21 pKa = 4.04 RR22 pKa = 11.84 SSWVHH27 pKa = 6.07 SEE29 pKa = 4.82 PDD31 pKa = 3.7 DD32 pKa = 4.48 QPDD35 pKa = 3.53 ALLSAGSLGVYY46 pKa = 9.74 NQDD49 pKa = 3.62 DD50 pKa = 4.45 NKK52 pKa = 10.72 NSSPVRR58 pKa = 11.84 MLGSDD63 pKa = 3.38 HH64 pKa = 6.35 GTIGCLPEE72 pKa = 4.42 QDD74 pKa = 4.27 ISPSLAPSAEE84 pKa = 4.27 SGCGVDD90 pKa = 5.15 HH91 pKa = 7.08 SLLPGEE97 pKa = 5.11 GDD99 pKa = 3.76 GQICSGNSS107 pKa = 2.92
Molecular weight: 11.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.714
IPC2_protein 3.834
IPC_protein 3.808
Toseland 3.592
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.986
Wikipedia 3.795
Rodwell 3.643
Grimsley 3.503
Solomon 3.808
Lehninger 3.77
Nozaki 3.948
DTASelect 4.215
Thurlkill 3.668
EMBOSS 3.795
Sillero 3.935
Patrickios 0.896
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.837
Protein with the highest isoelectric point:
>tr|A0A1R3IRN9|A0A1R3IRN9_COCAP Glutamine amidotransferase type 1 OS=Corchorus capsularis OX=210143 GN=CCACVL1_10306 PE=4 SV=1
MM1 pKa = 7.1 RR2 pKa = 11.84 AKK4 pKa = 9.27 WKK6 pKa = 9.53 KK7 pKa = 9.88 KK8 pKa = 8.64 RR9 pKa = 11.84 MRR11 pKa = 11.84 RR12 pKa = 11.84 LKK14 pKa = 10.08 RR15 pKa = 11.84 KK16 pKa = 8.21 RR17 pKa = 11.84 RR18 pKa = 11.84 KK19 pKa = 8.46 MRR21 pKa = 11.84 QRR23 pKa = 11.84 SKK25 pKa = 11.41
Molecular weight: 3.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.169
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.705
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.427
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.118
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
29098
0
29098
10081701
19
5351
346.5
38.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.829 ± 0.015
1.807 ± 0.007
5.357 ± 0.012
6.626 ± 0.017
4.196 ± 0.009
6.448 ± 0.017
2.313 ± 0.006
5.316 ± 0.01
6.362 ± 0.014
9.582 ± 0.018
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.435 ± 0.007
4.56 ± 0.012
4.998 ± 0.014
3.774 ± 0.01
5.272 ± 0.012
8.857 ± 0.019
4.805 ± 0.01
6.42 ± 0.011
1.286 ± 0.006
2.757 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here