Aneurinibacillus migulanus (Bacillus migulanus)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Aneurinibacillus group; Aneurinibacillus

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5649 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M0H6S5|A0A0M0H6S5_ANEMI Uncharacterized protein OS=Aneurinibacillus migulanus OX=47500 GN=AF333_22475 PE=4 SV=1
MM1 pKa = 7.28QSPGFGGVGCLCIRR15 pKa = 11.84DD16 pKa = 4.04GLFCSRR22 pKa = 11.84PVPVFDD28 pKa = 3.13IQGYY32 pKa = 10.0AEE34 pKa = 4.06YY35 pKa = 10.6RR36 pKa = 11.84DD37 pKa = 3.59IASKK41 pKa = 10.66EE42 pKa = 3.88RR43 pKa = 11.84TEE45 pKa = 4.2ADD47 pKa = 3.13LPGEE51 pKa = 4.21VGGWANIASFAMTFDD66 pKa = 3.26EE67 pKa = 4.9FDD69 pKa = 3.55PFEE72 pKa = 4.47EE73 pKa = 4.43CFEE76 pKa = 4.41ASSMTNN82 pKa = 2.89

Molecular weight: 8.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D1UXD1|A0A0D1UXD1_ANEMI Ribonucleoside-diphosphate reductase OS=Aneurinibacillus migulanus OX=47500 GN=AF333_22810 PE=3 SV=1
MM1 pKa = 7.61KK2 pKa = 8.8PTFNPNNRR10 pKa = 11.84KK11 pKa = 9.18RR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.69VHH16 pKa = 5.46GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.19NGRR28 pKa = 11.84KK29 pKa = 8.89VLANRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.69GRR39 pKa = 11.84KK40 pKa = 8.69VLSAA44 pKa = 4.05

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5648

1

5649

1578181

28

4450

279.4

31.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.719 ± 0.039

0.862 ± 0.011

4.754 ± 0.025

7.439 ± 0.041

4.265 ± 0.027

7.044 ± 0.031

2.136 ± 0.017

7.401 ± 0.032

6.215 ± 0.035

9.743 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.933 ± 0.016

3.828 ± 0.022

3.871 ± 0.019

3.877 ± 0.024

4.955 ± 0.03

5.711 ± 0.027

5.433 ± 0.023

7.187 ± 0.027

1.099 ± 0.012

3.526 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski