Yersinia phage fHe-Yen3-01
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L7DQG9|A0A1L7DQG9_9CAUD Uncharacterized protein OS=Yersinia phage fHe-Yen3-01 OX=1932893 GN=fHeYen301_13 PE=4 SV=1
MM1 pKa = 7.79 NIQQIIDD8 pKa = 3.43 VVGAVTAGRR17 pKa = 11.84 SFYY20 pKa = 10.45 IAGGFARR27 pKa = 11.84 DD28 pKa = 3.03 LAYY31 pKa = 10.37 CVKK34 pKa = 10.21 PKK36 pKa = 11.13 DD37 pKa = 3.47 MDD39 pKa = 4.34 LLFTADD45 pKa = 4.18 ARR47 pKa = 11.84 DD48 pKa = 3.63 SNADD52 pKa = 3.39 VFADD56 pKa = 3.32 MCDD59 pKa = 3.56 YY60 pKa = 11.6 SDD62 pKa = 4.51 ALSKK66 pKa = 10.83 LGYY69 pKa = 9.84 KK70 pKa = 9.09 VAIYY74 pKa = 9.74 QAYY77 pKa = 9.31 DD78 pKa = 3.28 QSDD81 pKa = 3.55 KK82 pKa = 11.43 PGDD85 pKa = 3.58 FNDD88 pKa = 4.24 RR89 pKa = 11.84 LFGCMKK95 pKa = 10.48 VEE97 pKa = 4.18 GFDD100 pKa = 3.54 VDD102 pKa = 3.71 VDD104 pKa = 4.34 LLFSKK109 pKa = 10.9 YY110 pKa = 9.89 EE111 pKa = 4.24 SIGEE115 pKa = 4.04 ILEE118 pKa = 4.67 HH119 pKa = 7.0 FDD121 pKa = 3.76 FNINQFYY128 pKa = 10.16 ILPYY132 pKa = 9.99 SKK134 pKa = 10.11 PIYY137 pKa = 10.31 ARR139 pKa = 11.84 GVKK142 pKa = 9.76 PDD144 pKa = 3.71 TLVQLKK150 pKa = 10.27 DD151 pKa = 3.32 VSEE154 pKa = 3.97 QRR156 pKa = 11.84 KK157 pKa = 9.42 EE158 pKa = 3.68 YY159 pKa = 8.01 MTAKK163 pKa = 10.6 FEE165 pKa = 4.31 VLNNQPVPEE174 pKa = 4.83 GCLVRR179 pKa = 11.84 PAHH182 pKa = 5.76 IPEE185 pKa = 4.07 RR186 pKa = 11.84 AEE188 pKa = 4.02 PYY190 pKa = 10.37 RR191 pKa = 11.84 LIITDD196 pKa = 3.63 WNQPYY201 pKa = 9.01 VWCYY205 pKa = 7.17 VTGEE209 pKa = 3.79 QTYY212 pKa = 9.96 EE213 pKa = 3.99 VFGGLTWEE221 pKa = 4.25 EE222 pKa = 3.35 VDD224 pKa = 3.31 IARR227 pKa = 11.84 ACGQLKK233 pKa = 9.64 VHH235 pKa = 6.88 DD236 pKa = 4.75 ARR238 pKa = 11.84 PNINRR243 pKa = 11.84 ALDD246 pKa = 3.67 YY247 pKa = 11.42 DD248 pKa = 3.89 NGEE251 pKa = 3.95 HH252 pKa = 6.95 HH253 pKa = 7.22 LL254 pKa = 4.13
Molecular weight: 29.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.47
IPC2_protein 4.609
IPC_protein 4.571
Toseland 4.393
ProMoST 4.698
Dawson 4.546
Bjellqvist 4.685
Wikipedia 4.444
Rodwell 4.406
Grimsley 4.304
Solomon 4.533
Lehninger 4.495
Nozaki 4.647
DTASelect 4.876
Thurlkill 4.418
EMBOSS 4.457
Sillero 4.698
Patrickios 3.236
IPC_peptide 4.546
IPC2_peptide 4.685
IPC2.peptide.svr19 4.655
Protein with the highest isoelectric point:
>tr|A0A1L7DQF2|A0A1L7DQF2_9CAUD Uncharacterized protein OS=Yersinia phage fHe-Yen3-01 OX=1932893 GN=fHeYen301_15 PE=4 SV=1
MM1 pKa = 7.26 VNLHH5 pKa = 6.3 IIFRR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 LTDD14 pKa = 3.59 FLFNPKK20 pKa = 9.74 RR21 pKa = 11.84 PNVVTFKK28 pKa = 10.85 NVYY31 pKa = 6.84 ITHH34 pKa = 6.18 RR35 pKa = 11.84 TGGLEE40 pKa = 4.04 VKK42 pKa = 10.67 HH43 pKa = 6.62 NDD45 pKa = 2.86 GKK47 pKa = 10.85 VYY49 pKa = 10.02 IYY51 pKa = 10.65 SWHH54 pKa = 5.55 TLNRR58 pKa = 11.84 VRR60 pKa = 11.84 TEE62 pKa = 3.65 HH63 pKa = 6.63 CC64 pKa = 3.81
Molecular weight: 7.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.321
IPC2_protein 9.78
IPC_protein 10.482
Toseland 10.511
ProMoST 10.204
Dawson 10.657
Bjellqvist 10.365
Wikipedia 10.862
Rodwell 10.935
Grimsley 10.716
Solomon 10.73
Lehninger 10.701
Nozaki 10.496
DTASelect 10.35
Thurlkill 10.526
EMBOSS 10.906
Sillero 10.584
Patrickios 10.716
IPC_peptide 10.73
IPC2_peptide 9.341
IPC2.peptide.svr19 8.499
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
56
0
56
13312
42
1259
237.7
26.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.638 ± 0.593
1.157 ± 0.18
6.468 ± 0.181
6.227 ± 0.272
3.538 ± 0.194
7.459 ± 0.282
2.021 ± 0.216
4.995 ± 0.264
6.363 ± 0.256
8.075 ± 0.372
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.102 ± 0.21
4.319 ± 0.217
3.839 ± 0.227
4.132 ± 0.341
5.364 ± 0.215
5.95 ± 0.264
5.566 ± 0.249
6.753 ± 0.349
1.337 ± 0.127
3.696 ± 0.219
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here