Papaya leaf curl virus
Average proteome isoelectric point is 7.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q6KF66|Q6KF66_9GEMI Transcriptional activator protein OS=Papaya leaf curl virus OX=53260 GN=C2 PE=3 SV=1
MM1 pKa = 7.45 PRR3 pKa = 11.84 INSFCVNAKK12 pKa = 10.44 NIFLTYY18 pKa = 8.78 PKK20 pKa = 10.44 CPIPKK25 pKa = 9.32 EE26 pKa = 3.87 QMLEE30 pKa = 3.65 ILQSINCPSDD40 pKa = 3.1 KK41 pKa = 10.94 LFIRR45 pKa = 11.84 VAQEE49 pKa = 3.46 KK50 pKa = 9.49 HH51 pKa = 5.19 QDD53 pKa = 3.38 GSLHH57 pKa = 5.16 IHH59 pKa = 6.91 ALIQFKK65 pKa = 10.72 GKK67 pKa = 10.47 SKK69 pKa = 10.22 FRR71 pKa = 11.84 NPRR74 pKa = 11.84 HH75 pKa = 6.17 FDD77 pKa = 3.03 VTHH80 pKa = 7.16 PNNSTQFHH88 pKa = 7.21 PNFQGAKK95 pKa = 9.08 SSSDD99 pKa = 3.06 VKK101 pKa = 11.23 SYY103 pKa = 10.83 IEE105 pKa = 4.63 KK106 pKa = 10.79 DD107 pKa = 2.7 SDD109 pKa = 4.06 YY110 pKa = 10.96 IDD112 pKa = 3.35 WGTFQIDD119 pKa = 3.03 GRR121 pKa = 11.84 SARR124 pKa = 11.84 GGQQTANDD132 pKa = 3.91 AAAEE136 pKa = 3.92 ALNAGSAEE144 pKa = 3.87 AALAIIRR151 pKa = 11.84 EE152 pKa = 4.23 KK153 pKa = 10.87 LPKK156 pKa = 10.46 DD157 pKa = 4.77 FIFQYY162 pKa = 11.21 HH163 pKa = 5.47 NLKK166 pKa = 10.81 CNLEE170 pKa = 4.44 RR171 pKa = 11.84 IYY173 pKa = 10.07 TPPMEE178 pKa = 4.77 VYY180 pKa = 10.56 VSPFSSSSFDD190 pKa = 3.41 QVPEE194 pKa = 3.98 EE195 pKa = 4.13 LEE197 pKa = 3.58 EE198 pKa = 3.9 WAAEE202 pKa = 4.09 NVVGDD207 pKa = 3.89 AARR210 pKa = 11.84 ANRR213 pKa = 11.84 PISIVIEE220 pKa = 4.23 GDD222 pKa = 3.2 SRR224 pKa = 11.84 TGKK227 pKa = 8.52 TMWARR232 pKa = 11.84 SLGPHH237 pKa = 6.71 NYY239 pKa = 10.18 LCGHH243 pKa = 7.38 LDD245 pKa = 4.18 LSPKK249 pKa = 10.02 VYY251 pKa = 10.89 SNEE254 pKa = 3.02 AWYY257 pKa = 10.82 NVIDD261 pKa = 5.29 DD262 pKa = 4.41 VDD264 pKa = 3.61 PHH266 pKa = 5.85 YY267 pKa = 11.0 LKK269 pKa = 10.7 HH270 pKa = 6.2 FKK272 pKa = 10.7 EE273 pKa = 4.46 FMGAQRR279 pKa = 11.84 DD280 pKa = 3.81 WQSNTKK286 pKa = 9.6 YY287 pKa = 10.57 GKK289 pKa = 9.12 PVQIKK294 pKa = 10.41 GGIPTIFLCNPGPNSSYY311 pKa = 11.34 KK312 pKa = 10.62 EE313 pKa = 3.85 FLDD316 pKa = 3.82 EE317 pKa = 4.66 EE318 pKa = 4.61 KK319 pKa = 11.18 NSALKK324 pKa = 10.53 NWALKK329 pKa = 10.24 NAIFVTLEE337 pKa = 3.93 GPLYY341 pKa = 10.82 SGSNQSAAQASQEE354 pKa = 3.75 GDD356 pKa = 3.29 QTSEE360 pKa = 3.96 SS361 pKa = 3.75
Molecular weight: 40.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.834
IPC2_protein 5.792
IPC_protein 5.855
Toseland 6.262
ProMoST 6.211
Dawson 6.148
Bjellqvist 6.135
Wikipedia 6.16
Rodwell 6.135
Grimsley 6.415
Solomon 6.148
Lehninger 6.135
Nozaki 6.415
DTASelect 6.605
Thurlkill 6.62
EMBOSS 6.59
Sillero 6.517
Patrickios 4.126
IPC_peptide 6.16
IPC2_peptide 6.478
IPC2.peptide.svr19 6.468
Protein with the highest isoelectric point:
>tr|Q6KF69|Q6KF69_9GEMI Protein V2 OS=Papaya leaf curl virus OX=53260 GN=V1 PE=3 SV=1
MM1 pKa = 7.71 SKK3 pKa = 10.35 RR4 pKa = 11.84 PADD7 pKa = 3.87 IIISTPASKK16 pKa = 10.3 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFDD24 pKa = 3.02 SPYY27 pKa = 8.76 VSRR30 pKa = 11.84 VAAPIVRR37 pKa = 11.84 VTKK40 pKa = 10.71 AKK42 pKa = 10.29 AWANRR47 pKa = 11.84 PMNRR51 pKa = 11.84 KK52 pKa = 7.87 PRR54 pKa = 11.84 MYY56 pKa = 10.68 RR57 pKa = 11.84 MYY59 pKa = 10.44 RR60 pKa = 11.84 SPDD63 pKa = 3.21 VPRR66 pKa = 11.84 GCEE69 pKa = 4.34 GPCKK73 pKa = 10.01 VQSFEE78 pKa = 4.1 SRR80 pKa = 11.84 HH81 pKa = 6.13 DD82 pKa = 3.26 IQHH85 pKa = 6.35 IGKK88 pKa = 8.97 VMCVSDD94 pKa = 3.59 VTRR97 pKa = 11.84 GTGLTHH103 pKa = 7.09 RR104 pKa = 11.84 VGKK107 pKa = 9.66 RR108 pKa = 11.84 FCVKK112 pKa = 9.94 SVYY115 pKa = 10.52 VLGKK119 pKa = 9.41 IWMDD123 pKa = 3.35 EE124 pKa = 4.04 NIKK127 pKa = 9.32 TKK129 pKa = 10.6 NHH131 pKa = 5.78 TNSVMFFLVRR141 pKa = 11.84 DD142 pKa = 3.84 RR143 pKa = 11.84 RR144 pKa = 11.84 PVDD147 pKa = 3.13 KK148 pKa = 10.28 PQDD151 pKa = 3.56 FGEE154 pKa = 4.33 VFNMFDD160 pKa = 4.29 NEE162 pKa = 4.11 PSTATVKK169 pKa = 10.38 NVHH172 pKa = 6.5 RR173 pKa = 11.84 DD174 pKa = 3.15 RR175 pKa = 11.84 YY176 pKa = 8.77 QVLRR180 pKa = 11.84 KK181 pKa = 9.41 WYY183 pKa = 8.08 ATVTGGQYY191 pKa = 10.85 ASKK194 pKa = 10.24 EE195 pKa = 3.73 QALVKK200 pKa = 10.65 KK201 pKa = 9.58 FIRR204 pKa = 11.84 VNNYY208 pKa = 7.74 VVYY211 pKa = 9.96 NQQEE215 pKa = 3.8 AGKK218 pKa = 10.08 YY219 pKa = 8.01 EE220 pKa = 4.07 NHH222 pKa = 6.29 SEE224 pKa = 4.11 NALMLYY230 pKa = 7.58 MACTHH235 pKa = 7.06 ASNPVYY241 pKa = 9.86 ATLKK245 pKa = 9.47 IRR247 pKa = 11.84 IYY249 pKa = 10.64 FYY251 pKa = 11.34 DD252 pKa = 3.32 SVTNN256 pKa = 3.98
Molecular weight: 29.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.251
IPC2_protein 9.516
IPC_protein 9.677
Toseland 10.101
ProMoST 9.823
Dawson 10.306
Bjellqvist 10.014
Wikipedia 10.496
Rodwell 10.687
Grimsley 10.394
Solomon 10.335
Lehninger 10.306
Nozaki 10.116
DTASelect 9.999
Thurlkill 10.16
EMBOSS 10.511
Sillero 10.233
Patrickios 10.189
IPC_peptide 10.335
IPC2_peptide 8.843
IPC2.peptide.svr19 8.483
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1120
85
361
186.7
21.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.982 ± 0.879
2.232 ± 0.57
4.643 ± 0.498
4.821 ± 0.613
4.375 ± 0.541
4.554 ± 0.541
3.75 ± 0.592
5.714 ± 1.016
5.625 ± 0.934
6.518 ± 0.821
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.768 ± 0.767
5.625 ± 0.832
6.429 ± 0.875
5.0 ± 0.501
6.696 ± 1.204
8.661 ± 1.731
4.911 ± 0.829
6.875 ± 1.392
1.339 ± 0.236
3.482 ± 0.704
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here