Kutzneria buriramensis
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10965 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3E0G557|A0A3E0G557_9PSEU Uncharacterized protein OS=Kutzneria buriramensis OX=1045776 GN=BCF44_1412 PE=4 SV=1
MM1 pKa = 7.61 AVQLDD6 pKa = 3.99 PQLLEE11 pKa = 4.0 ILRR14 pKa = 11.84 CPSPDD19 pKa = 3.44 HH20 pKa = 6.89 APLRR24 pKa = 11.84 PGTPDD29 pKa = 4.35 DD30 pKa = 5.0 ADD32 pKa = 5.07 ADD34 pKa = 3.85 MLTCTEE40 pKa = 4.47 CGRR43 pKa = 11.84 GYY45 pKa = 9.79 PVQDD49 pKa = 4.97 GIPVLLLDD57 pKa = 4.41 EE58 pKa = 5.27 AVPPPGGLAGG68 pKa = 3.78
Molecular weight: 7.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.859
IPC_protein 3.77
Toseland 3.567
ProMoST 3.973
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.77
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.719
Nozaki 3.923
DTASelect 4.164
Thurlkill 3.656
EMBOSS 3.77
Sillero 3.897
Patrickios 1.914
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.816
Protein with the highest isoelectric point:
>tr|A0A3E0HU98|A0A3E0HU98_9PSEU Uncharacterized protein OS=Kutzneria buriramensis OX=1045776 GN=BCF44_104248 PE=4 SV=1
MM1 pKa = 7.53 SKK3 pKa = 10.53 GKK5 pKa = 8.66 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 AKK17 pKa = 8.7 THH19 pKa = 5.15 GFRR22 pKa = 11.84 LRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AILAARR37 pKa = 11.84 RR38 pKa = 11.84 GKK40 pKa = 10.37 GRR42 pKa = 11.84 ARR44 pKa = 11.84 LSAA47 pKa = 3.91
Molecular weight: 5.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.427
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10965
0
10965
3593623
25
8516
327.7
35.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.216 ± 0.033
0.846 ± 0.008
6.232 ± 0.019
4.999 ± 0.026
2.841 ± 0.013
8.982 ± 0.022
2.296 ± 0.015
3.468 ± 0.013
1.94 ± 0.016
10.466 ± 0.033
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.751 ± 0.008
2.162 ± 0.018
5.821 ± 0.022
3.12 ± 0.017
7.587 ± 0.029
5.276 ± 0.019
6.321 ± 0.026
9.004 ± 0.025
1.607 ± 0.01
2.066 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here