Kutzneria buriramensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Kutzneria

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10965 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A3E0G557|A0A3E0G557_9PSEU Uncharacterized protein OS=Kutzneria buriramensis OX=1045776 GN=BCF44_1412 PE=4 SV=1
MM1 pKa = 7.61AVQLDD6 pKa = 3.99PQLLEE11 pKa = 4.0ILRR14 pKa = 11.84CPSPDD19 pKa = 3.44HH20 pKa = 6.89APLRR24 pKa = 11.84PGTPDD29 pKa = 4.35DD30 pKa = 5.0ADD32 pKa = 5.07ADD34 pKa = 3.85MLTCTEE40 pKa = 4.47CGRR43 pKa = 11.84GYY45 pKa = 9.79PVQDD49 pKa = 4.97GIPVLLLDD57 pKa = 4.41EE58 pKa = 5.27AVPPPGGLAGG68 pKa = 3.78

Molecular weight:
7.09 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A3E0HU98|A0A3E0HU98_9PSEU Uncharacterized protein OS=Kutzneria buriramensis OX=1045776 GN=BCF44_104248 PE=4 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.53GKK5 pKa = 8.66RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84AKK17 pKa = 8.7THH19 pKa = 5.15GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AILAARR37 pKa = 11.84RR38 pKa = 11.84GKK40 pKa = 10.37GRR42 pKa = 11.84ARR44 pKa = 11.84LSAA47 pKa = 3.91

Molecular weight:
5.42 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10965

0

10965

3593623

25

8516

327.7

35.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.216 ± 0.033

0.846 ± 0.008

6.232 ± 0.019

4.999 ± 0.026

2.841 ± 0.013

8.982 ± 0.022

2.296 ± 0.015

3.468 ± 0.013

1.94 ± 0.016

10.466 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.751 ± 0.008

2.162 ± 0.018

5.821 ± 0.022

3.12 ± 0.017

7.587 ± 0.029

5.276 ± 0.019

6.321 ± 0.026

9.004 ± 0.025

1.607 ± 0.01

2.066 ± 0.014

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski