bacterium AMD01

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 7.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 639 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q0AKS7|A0A4Q0AKS7_9BACT Uncharacterized protein (Fragment) OS=bacterium AMD01 OX=2506947 GN=EOT04_00760 PE=4 SV=1
MM1 pKa = 7.21NQLLNDD7 pKa = 3.63NANADD12 pKa = 3.61KK13 pKa = 11.11VYY15 pKa = 10.59DD16 pKa = 3.95CVMVGAGPAALAAAVYY32 pKa = 7.45TARR35 pKa = 11.84EE36 pKa = 4.05NIEE39 pKa = 3.97TVLYY43 pKa = 9.47EE44 pKa = 3.86RR45 pKa = 11.84GVIGGLAAVTDD56 pKa = 4.16KK57 pKa = 11.36VDD59 pKa = 2.97NN60 pKa = 4.17

Molecular weight: 6.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q0AIZ8|A0A4Q0AIZ8_9BACT Hsp20/alpha crystallin family protein OS=bacterium AMD01 OX=2506947 GN=EOT04_02240 PE=3 SV=1
MM1 pKa = 8.03PKK3 pKa = 8.99RR4 pKa = 11.84TYY6 pKa = 9.7QPKK9 pKa = 9.45KK10 pKa = 7.36RR11 pKa = 11.84TRR13 pKa = 11.84AKK15 pKa = 9.29VHH17 pKa = 5.67GFRR20 pKa = 11.84ARR22 pKa = 11.84MASKK26 pKa = 9.21TGQRR30 pKa = 11.84VLKK33 pKa = 10.42SRR35 pKa = 11.84RR36 pKa = 11.84QKK38 pKa = 9.82GRR40 pKa = 11.84KK41 pKa = 8.36VLTVGRR47 pKa = 4.21

Molecular weight: 5.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

639

0

639

184683

38

1213

289.0

31.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.602 ± 0.12

0.593 ± 0.03

5.142 ± 0.07

5.261 ± 0.105

3.495 ± 0.078

7.643 ± 0.094

1.782 ± 0.045

5.481 ± 0.071

5.35 ± 0.085

10.829 ± 0.141

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.019 ± 0.042

3.611 ± 0.07

4.838 ± 0.076

4.259 ± 0.067

6.61 ± 0.091

6.243 ± 0.076

4.862 ± 0.072

7.22 ± 0.09

1.165 ± 0.046

2.994 ± 0.056
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski