Amino acid dipepetide frequency for bacterium AMD01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.621AlaAla: 15.621 ± 0.512
0.569AlaCys: 0.569 ± 0.058
5.453AlaAsp: 5.453 ± 0.167
5.924AlaGlu: 5.924 ± 0.206
3.043AlaPhe: 3.043 ± 0.128
9.173AlaGly: 9.173 ± 0.256
1.662AlaHis: 1.662 ± 0.098
5.518AlaIle: 5.518 ± 0.195
5.967AlaLys: 5.967 ± 0.187
10.678AlaLeu: 10.678 ± 0.247
2.215AlaMet: 2.215 ± 0.11
3.016AlaAsn: 3.016 ± 0.125
3.991AlaPro: 3.991 ± 0.178
3.633AlaGln: 3.633 ± 0.147
6.563AlaArg: 6.563 ± 0.218
5.848AlaSer: 5.848 ± 0.206
4.862AlaThr: 4.862 ± 0.173
8.279AlaVal: 8.279 ± 0.279
1.207AlaTrp: 1.207 ± 0.088
2.805AlaTyr: 2.805 ± 0.118
0.0AlaXaa: 0.0 ± 0.0
Cys
0.439CysAla: 0.439 ± 0.049
0.027CysCys: 0.027 ± 0.015
0.325CysAsp: 0.325 ± 0.042
0.26CysGlu: 0.26 ± 0.04
0.222CysPhe: 0.222 ± 0.035
0.628CysGly: 0.628 ± 0.066
0.135CysHis: 0.135 ± 0.027
0.222CysIle: 0.222 ± 0.03
0.19CysLys: 0.19 ± 0.033
0.601CysLeu: 0.601 ± 0.069
0.076CysMet: 0.076 ± 0.019
0.162CysAsn: 0.162 ± 0.032
0.466CysPro: 0.466 ± 0.052
0.309CysGln: 0.309 ± 0.043
0.601CysArg: 0.601 ± 0.067
0.433CysSer: 0.433 ± 0.063
0.217CysThr: 0.217 ± 0.035
0.352CysVal: 0.352 ± 0.04
0.103CysTrp: 0.103 ± 0.027
0.168CysTyr: 0.168 ± 0.026
0.0CysXaa: 0.0 ± 0.0
Asp
4.332AspAla: 4.332 ± 0.142
0.303AspCys: 0.303 ± 0.041
2.756AspAsp: 2.756 ± 0.129
3.276AspGlu: 3.276 ± 0.148
2.334AspPhe: 2.334 ± 0.109
4.072AspGly: 4.072 ± 0.179
0.893AspHis: 0.893 ± 0.074
3.222AspIle: 3.222 ± 0.14
3.048AspLys: 3.048 ± 0.132
4.819AspLeu: 4.819 ± 0.183
0.969AspMet: 0.969 ± 0.073
1.933AspAsn: 1.933 ± 0.094
2.762AspPro: 2.762 ± 0.12
2.399AspGln: 2.399 ± 0.107
2.978AspArg: 2.978 ± 0.15
2.94AspSer: 2.94 ± 0.129
2.453AspThr: 2.453 ± 0.116
3.335AspVal: 3.335 ± 0.158
0.785AspTrp: 0.785 ± 0.069
1.814AspTyr: 1.814 ± 0.095
0.0AspXaa: 0.0 ± 0.0
Glu
5.783GluAla: 5.783 ± 0.208
0.347GluCys: 0.347 ± 0.04
2.518GluAsp: 2.518 ± 0.13
3.097GluGlu: 3.097 ± 0.162
1.668GluPhe: 1.668 ± 0.089
2.94GluGly: 2.94 ± 0.139
1.143GluHis: 1.143 ± 0.081
3.314GluIle: 3.314 ± 0.134
2.864GluLys: 2.864 ± 0.129
6.341GluLeu: 6.341 ± 0.208
1.05GluMet: 1.05 ± 0.079
1.749GluAsn: 1.749 ± 0.096
2.052GluPro: 2.052 ± 0.122
2.545GluGln: 2.545 ± 0.137
3.92GluArg: 3.92 ± 0.156
2.778GluSer: 2.778 ± 0.141
2.81GluThr: 2.81 ± 0.126
3.714GluVal: 3.714 ± 0.166
0.471GluTrp: 0.471 ± 0.053
1.505GluTyr: 1.505 ± 0.085
0.0GluXaa: 0.0 ± 0.0
Phe
3.46PheAla: 3.46 ± 0.162
0.314PheCys: 0.314 ± 0.043
2.155PheAsp: 2.155 ± 0.106
1.841PheGlu: 1.841 ± 0.105
1.348PhePhe: 1.348 ± 0.099
3.141PheGly: 3.141 ± 0.15
0.46PheHis: 0.46 ± 0.05
1.987PheIle: 1.987 ± 0.117
2.209PheLys: 2.209 ± 0.101
3.195PheLeu: 3.195 ± 0.141
0.877PheMet: 0.877 ± 0.065
1.635PheAsn: 1.635 ± 0.105
0.969PhePro: 0.969 ± 0.083
0.888PheGln: 0.888 ± 0.068
1.668PheArg: 1.668 ± 0.093
2.561PheSer: 2.561 ± 0.13
1.684PheThr: 1.684 ± 0.097
2.55PheVal: 2.55 ± 0.126
0.698PheTrp: 0.698 ± 0.068
1.31PheTyr: 1.31 ± 0.1
0.0PheXaa: 0.0 ± 0.0
Gly
6.519GlyAla: 6.519 ± 0.221
0.531GlyCys: 0.531 ± 0.066
3.909GlyAsp: 3.909 ± 0.158
3.855GlyGlu: 3.855 ± 0.148
2.859GlyPhe: 2.859 ± 0.132
6.601GlyGly: 6.601 ± 0.279
1.381GlyHis: 1.381 ± 0.077
3.899GlyIle: 3.899 ± 0.155
4.294GlyLys: 4.294 ± 0.143
8.577GlyLeu: 8.577 ± 0.273
1.689GlyMet: 1.689 ± 0.084
2.615GlyAsn: 2.615 ± 0.133
2.615GlyPro: 2.615 ± 0.137
3.682GlyGln: 3.682 ± 0.132
5.55GlyArg: 5.55 ± 0.18
5.09GlySer: 5.09 ± 0.195
3.303GlyThr: 3.303 ± 0.149
5.577GlyVal: 5.577 ± 0.201
1.245GlyTrp: 1.245 ± 0.095
2.642GlyTyr: 2.642 ± 0.133
0.0GlyXaa: 0.0 ± 0.0
His
1.429HisAla: 1.429 ± 0.09
0.097HisCys: 0.097 ± 0.021
0.818HisAsp: 0.818 ± 0.055
1.002HisGlu: 1.002 ± 0.087
0.677HisPhe: 0.677 ± 0.053
1.494HisGly: 1.494 ± 0.095
0.536HisHis: 0.536 ± 0.063
1.191HisIle: 1.191 ± 0.085
0.828HisLys: 0.828 ± 0.074
1.873HisLeu: 1.873 ± 0.12
0.368HisMet: 0.368 ± 0.042
0.601HisAsn: 0.601 ± 0.061
1.061HisPro: 1.061 ± 0.079
0.893HisGln: 0.893 ± 0.067
1.121HisArg: 1.121 ± 0.082
1.007HisSer: 1.007 ± 0.068
0.834HisThr: 0.834 ± 0.075
1.143HisVal: 1.143 ± 0.067
0.249HisTrp: 0.249 ± 0.037
0.596HisTyr: 0.596 ± 0.062
0.0HisXaa: 0.0 ± 0.0
Ile
5.447IleAla: 5.447 ± 0.18
0.406IleCys: 0.406 ± 0.045
3.471IleAsp: 3.471 ± 0.147
3.693IleGlu: 3.693 ± 0.171
1.792IlePhe: 1.792 ± 0.112
4.462IleGly: 4.462 ± 0.16
0.742IleHis: 0.742 ± 0.066
3.557IleIle: 3.557 ± 0.155
3.243IleLys: 3.243 ± 0.141
4.603IleLeu: 4.603 ± 0.197
1.115IleMet: 1.115 ± 0.076
2.632IleAsn: 2.632 ± 0.12
1.944IlePro: 1.944 ± 0.104
1.581IleGln: 1.581 ± 0.09
3.173IleArg: 3.173 ± 0.144
3.601IleSer: 3.601 ± 0.136
3.184IleThr: 3.184 ± 0.143
4.11IleVal: 4.11 ± 0.175
0.466IleTrp: 0.466 ± 0.047
1.587IleTyr: 1.587 ± 0.09
0.0IleXaa: 0.0 ± 0.0
Lys
4.613LysAla: 4.613 ± 0.193
0.249LysCys: 0.249 ± 0.035
2.778LysAsp: 2.778 ± 0.141
2.724LysGlu: 2.724 ± 0.176
1.402LysPhe: 1.402 ± 0.088
2.837LysGly: 2.837 ± 0.159
1.045LysHis: 1.045 ± 0.073
3.395LysIle: 3.395 ± 0.168
3.52LysLys: 3.52 ± 0.168
5.918LysLeu: 5.918 ± 0.193
1.202LysMet: 1.202 ± 0.082
2.512LysAsn: 2.512 ± 0.14
2.702LysPro: 2.702 ± 0.15
2.783LysGln: 2.783 ± 0.111
4.001LysArg: 4.001 ± 0.141
3.151LysSer: 3.151 ± 0.137
3.612LysThr: 3.612 ± 0.153
3.373LysVal: 3.373 ± 0.144
0.325LysTrp: 0.325 ± 0.036
1.359LysTyr: 1.359 ± 0.09
0.0LysXaa: 0.0 ± 0.0
Leu
13.147LeuAla: 13.147 ± 0.373
0.579LeuCys: 0.579 ± 0.065
5.523LeuAsp: 5.523 ± 0.173
5.675LeuGlu: 5.675 ± 0.188
3.622LeuPhe: 3.622 ± 0.175
7.624LeuGly: 7.624 ± 0.233
1.76LeuHis: 1.76 ± 0.112
5.772LeuIle: 5.772 ± 0.223
6.319LeuLys: 6.319 ± 0.208
11.485LeuLeu: 11.485 ± 0.397
2.106LeuMet: 2.106 ± 0.102
4.153LeuAsn: 4.153 ± 0.159
5.859LeuPro: 5.859 ± 0.196
3.525LeuGln: 3.525 ± 0.155
6.249LeuArg: 6.249 ± 0.194
7.57LeuSer: 7.57 ± 0.174
5.604LeuThr: 5.604 ± 0.169
7.748LeuVal: 7.748 ± 0.224
1.11LeuTrp: 1.11 ± 0.088
2.859LeuTyr: 2.859 ± 0.112
0.0LeuXaa: 0.0 ± 0.0
Met
2.339MetAla: 2.339 ± 0.121
0.049MetCys: 0.049 ± 0.018
0.834MetAsp: 0.834 ± 0.076
0.937MetGlu: 0.937 ± 0.082
0.639MetPhe: 0.639 ± 0.067
1.159MetGly: 1.159 ± 0.085
0.319MetHis: 0.319 ± 0.041
1.137MetIle: 1.137 ± 0.062
1.083MetLys: 1.083 ± 0.071
2.22MetLeu: 2.22 ± 0.122
0.439MetMet: 0.439 ± 0.051
0.845MetAsn: 0.845 ± 0.064
1.218MetPro: 1.218 ± 0.1
0.758MetGln: 0.758 ± 0.073
1.354MetArg: 1.354 ± 0.084
1.543MetSer: 1.543 ± 0.084
1.191MetThr: 1.191 ± 0.082
1.581MetVal: 1.581 ± 0.093
0.119MetTrp: 0.119 ± 0.026
0.422MetTyr: 0.422 ± 0.052
0.0MetXaa: 0.0 ± 0.0
Asn
3.222AsnAla: 3.222 ± 0.139
0.303AsnCys: 0.303 ± 0.042
1.614AsnAsp: 1.614 ± 0.094
1.706AsnGlu: 1.706 ± 0.109
1.332AsnPhe: 1.332 ± 0.11
2.783AsnGly: 2.783 ± 0.149
0.704AsnHis: 0.704 ± 0.061
1.976AsnIle: 1.976 ± 0.107
1.684AsnLys: 1.684 ± 0.101
4.186AsnLeu: 4.186 ± 0.154
0.644AsnMet: 0.644 ± 0.061
1.451AsnAsn: 1.451 ± 0.118
2.545AsnPro: 2.545 ± 0.114
1.846AsnGln: 1.846 ± 0.117
2.724AsnArg: 2.724 ± 0.132
2.101AsnSer: 2.101 ± 0.105
1.354AsnThr: 1.354 ± 0.095
2.16AsnVal: 2.16 ± 0.121
0.514AsnTrp: 0.514 ± 0.062
1.262AsnTyr: 1.262 ± 0.083
0.0AsnXaa: 0.0 ± 0.0
Pro
6.189ProAla: 6.189 ± 0.258
0.211ProCys: 0.211 ± 0.037
2.772ProAsp: 2.772 ± 0.127
3.054ProGlu: 3.054 ± 0.172
1.478ProPhe: 1.478 ± 0.085
4.001ProGly: 4.001 ± 0.178
0.872ProHis: 0.872 ± 0.081
1.906ProIle: 1.906 ± 0.117
2.502ProLys: 2.502 ± 0.13
4.527ProLeu: 4.527 ± 0.177
0.828ProMet: 0.828 ± 0.063
1.484ProAsn: 1.484 ± 0.103
3.124ProPro: 3.124 ± 0.257
1.928ProGln: 1.928 ± 0.098
2.626ProArg: 2.626 ± 0.115
3.303ProSer: 3.303 ± 0.133
2.166ProThr: 2.166 ± 0.125
3.682ProVal: 3.682 ± 0.145
0.455ProTrp: 0.455 ± 0.052
1.272ProTyr: 1.272 ± 0.091
0.0ProXaa: 0.0 ± 0.0
Gln
4.77GlnAla: 4.77 ± 0.168
0.135GlnCys: 0.135 ± 0.031
1.987GlnAsp: 1.987 ± 0.102
2.177GlnGlu: 2.177 ± 0.11
1.278GlnPhe: 1.278 ± 0.067
2.247GlnGly: 2.247 ± 0.109
0.644GlnHis: 0.644 ± 0.062
2.594GlnIle: 2.594 ± 0.124
2.442GlnLys: 2.442 ± 0.132
5.063GlnLeu: 5.063 ± 0.17
0.888GlnMet: 0.888 ± 0.071
1.798GlnAsn: 1.798 ± 0.114
2.724GlnPro: 2.724 ± 0.13
2.09GlnGln: 2.09 ± 0.143
3.0GlnArg: 3.0 ± 0.144
2.377GlnSer: 2.377 ± 0.113
2.377GlnThr: 2.377 ± 0.109
2.567GlnVal: 2.567 ± 0.127
0.433GlnTrp: 0.433 ± 0.052
0.996GlnTyr: 0.996 ± 0.064
0.0GlnXaa: 0.0 ± 0.0
Arg
5.507ArgAla: 5.507 ± 0.184
0.33ArgCys: 0.33 ± 0.048
3.048ArgAsp: 3.048 ± 0.131
3.027ArgGlu: 3.027 ± 0.126
2.875ArgPhe: 2.875 ± 0.14
4.153ArgGly: 4.153 ± 0.159
1.716ArgHis: 1.716 ± 0.102
2.994ArgIle: 2.994 ± 0.158
2.453ArgLys: 2.453 ± 0.134
9.373ArgLeu: 9.373 ± 0.257
1.337ArgMet: 1.337 ± 0.086
1.836ArgAsn: 1.836 ± 0.108
3.52ArgPro: 3.52 ± 0.159
4.548ArgGln: 4.548 ± 0.184
5.972ArgArg: 5.972 ± 0.229
3.931ArgSer: 3.931 ± 0.172
2.301ArgThr: 2.301 ± 0.106
4.245ArgVal: 4.245 ± 0.148
0.666ArgTrp: 0.666 ± 0.069
2.263ArgTyr: 2.263 ± 0.114
0.0ArgXaa: 0.0 ± 0.0
Ser
6.384SerAla: 6.384 ± 0.211
0.33SerCys: 0.33 ± 0.043
3.233SerAsp: 3.233 ± 0.121
2.973SerGlu: 2.973 ± 0.152
2.399SerPhe: 2.399 ± 0.133
6.416SerGly: 6.416 ± 0.203
1.034SerHis: 1.034 ± 0.075
2.697SerIle: 2.697 ± 0.136
2.697SerLys: 2.697 ± 0.133
7.57SerLeu: 7.57 ± 0.189
1.191SerMet: 1.191 ± 0.088
1.776SerAsn: 1.776 ± 0.1
3.016SerPro: 3.016 ± 0.157
2.762SerGln: 2.762 ± 0.128
4.489SerArg: 4.489 ± 0.168
3.904SerSer: 3.904 ± 0.171
2.534SerThr: 2.534 ± 0.129
4.554SerVal: 4.554 ± 0.147
0.698SerTrp: 0.698 ± 0.061
1.771SerTyr: 1.771 ± 0.099
0.0SerXaa: 0.0 ± 0.0
Thr
5.756ThrAla: 5.756 ± 0.169
0.26ThrCys: 0.26 ± 0.039
2.54ThrAsp: 2.54 ± 0.108
2.003ThrGlu: 2.003 ± 0.108
1.484ThrPhe: 1.484 ± 0.095
4.131ThrGly: 4.131 ± 0.169
0.78ThrHis: 0.78 ± 0.067
3.113ThrIle: 3.113 ± 0.144
2.42ThrLys: 2.42 ± 0.119
4.684ThrLeu: 4.684 ± 0.176
0.796ThrMet: 0.796 ± 0.067
1.7ThrAsn: 1.7 ± 0.093
2.778ThrPro: 2.778 ± 0.129
1.457ThrGln: 1.457 ± 0.091
2.577ThrArg: 2.577 ± 0.11
3.005ThrSer: 3.005 ± 0.128
2.561ThrThr: 2.561 ± 0.157
4.467ThrVal: 4.467 ± 0.175
0.455ThrTrp: 0.455 ± 0.057
1.651ThrTyr: 1.651 ± 0.099
0.0ThrXaa: 0.0 ± 0.0
Val
7.489ValAla: 7.489 ± 0.199
0.466ValCys: 0.466 ± 0.046
3.379ValAsp: 3.379 ± 0.148
3.785ValGlu: 3.785 ± 0.135
2.61ValPhe: 2.61 ± 0.133
5.328ValGly: 5.328 ± 0.197
1.094ValHis: 1.094 ± 0.075
4.521ValIle: 4.521 ± 0.177
4.121ValLys: 4.121 ± 0.173
7.245ValLeu: 7.245 ± 0.2
1.662ValMet: 1.662 ± 0.107
2.74ValAsn: 2.74 ± 0.144
3.135ValPro: 3.135 ± 0.131
2.193ValGln: 2.193 ± 0.109
4.131ValArg: 4.131 ± 0.167
4.906ValSer: 4.906 ± 0.17
3.909ValThr: 3.909 ± 0.153
6.27ValVal: 6.27 ± 0.219
0.937ValTrp: 0.937 ± 0.071
2.285ValTyr: 2.285 ± 0.125
0.0ValXaa: 0.0 ± 0.0
Trp
0.856TrpAla: 0.856 ± 0.086
0.076TrpCys: 0.076 ± 0.021
0.476TrpAsp: 0.476 ± 0.053
0.336TrpGlu: 0.336 ± 0.051
0.509TrpPhe: 0.509 ± 0.055
0.698TrpGly: 0.698 ± 0.06
0.26TrpHis: 0.26 ± 0.04
0.476TrpIle: 0.476 ± 0.054
0.26TrpLys: 0.26 ± 0.036
2.009TrpLeu: 2.009 ± 0.136
0.265TrpMet: 0.265 ± 0.038
0.309TrpAsn: 0.309 ± 0.044
0.585TrpPro: 0.585 ± 0.062
0.953TrpGln: 0.953 ± 0.079
1.224TrpArg: 1.224 ± 0.09
0.731TrpSer: 0.731 ± 0.071
0.428TrpThr: 0.428 ± 0.051
0.569TrpVal: 0.569 ± 0.06
0.26TrpTrp: 0.26 ± 0.036
0.368TrpTyr: 0.368 ± 0.052
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.724TyrAla: 2.724 ± 0.129
0.352TyrCys: 0.352 ± 0.039
1.836TyrAsp: 1.836 ± 0.097
1.267TyrGlu: 1.267 ± 0.091
1.381TyrPhe: 1.381 ± 0.096
2.545TyrGly: 2.545 ± 0.135
0.639TyrHis: 0.639 ± 0.06
1.321TyrIle: 1.321 ± 0.085
1.359TyrLys: 1.359 ± 0.081
3.325TyrLeu: 3.325 ± 0.139
0.46TyrMet: 0.46 ± 0.051
1.164TyrAsn: 1.164 ± 0.091
1.316TyrPro: 1.316 ± 0.084
1.814TyrGln: 1.814 ± 0.112
2.177TyrArg: 2.177 ± 0.113
1.646TyrSer: 1.646 ± 0.093
1.235TyrThr: 1.235 ± 0.085
1.928TyrVal: 1.928 ± 0.112
0.449TyrTrp: 0.449 ± 0.045
1.007TyrTyr: 1.007 ± 0.074
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 639 proteins (184683 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski