Lactococcus phage CHPC148

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A650F7K8|A0A650F7K8_9CAUD Capsid and scaffold protein OS=Lactococcus phage CHPC148 OX=2675248 GN=CHPC148_000530 PE=4 SV=1
MM1 pKa = 7.36SVTVDD6 pKa = 4.12DD7 pKa = 6.04LLDD10 pKa = 3.62QLSEE14 pKa = 4.54DD15 pKa = 5.09DD16 pKa = 4.43DD17 pKa = 4.84RR18 pKa = 11.84KK19 pKa = 10.14PQLQIYY25 pKa = 9.45FDD27 pKa = 3.58TATAYY32 pKa = 10.31VKK34 pKa = 10.74NAVSSDD40 pKa = 3.41TVEE43 pKa = 3.66

Molecular weight: 4.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A650ESM8|A0A650ESM8_9CAUD Uncharacterized protein OS=Lactococcus phage CHPC148 OX=2675248 GN=CHPC148_000551 PE=4 SV=1
MM1 pKa = 7.61SKK3 pKa = 10.23LQSSLILSSRR13 pKa = 11.84IRR15 pKa = 11.84NNSTLAKK22 pKa = 9.47PPWAVVSYY30 pKa = 9.93LVKK33 pKa = 10.87AGGWRR38 pKa = 11.84NDD40 pKa = 3.57RR41 pKa = 11.84KK42 pKa = 8.94VHH44 pKa = 6.0EE45 pKa = 4.51YY46 pKa = 10.4SVPAHH51 pKa = 6.12KK52 pKa = 10.21HH53 pKa = 4.43SLPAFNLKK61 pKa = 10.4NKK63 pKa = 7.63TT64 pKa = 3.46

Molecular weight: 7.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

10277

43

897

205.5

23.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.617 ± 0.706

0.555 ± 0.124

6.383 ± 0.325

6.782 ± 0.43

3.98 ± 0.27

6.568 ± 0.493

1.362 ± 0.174

6.86 ± 0.322

9.088 ± 0.491

7.843 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.793 ± 0.194

6.364 ± 0.269

2.53 ± 0.291

4.661 ± 0.309

3.62 ± 0.383

7.035 ± 0.323

6.159 ± 0.516

5.858 ± 0.304

1.314 ± 0.156

3.629 ± 0.214
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski