Amino acid dipepetide frequency for Lactococcus phage CHPC148

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.742AlaAla: 5.742 ± 1.724
0.195AlaCys: 0.195 ± 0.134
4.768AlaAsp: 4.768 ± 0.701
4.963AlaGlu: 4.963 ± 0.985
2.044AlaPhe: 2.044 ± 0.521
4.574AlaGly: 4.574 ± 0.98
0.876AlaHis: 0.876 ± 0.363
5.255AlaIle: 5.255 ± 0.786
5.06AlaLys: 5.06 ± 0.979
4.768AlaLeu: 4.768 ± 0.665
2.238AlaMet: 2.238 ± 0.42
3.503AlaAsn: 3.503 ± 0.549
1.362AlaPro: 1.362 ± 0.476
3.698AlaGln: 3.698 ± 1.104
2.044AlaArg: 2.044 ± 0.617
3.99AlaSer: 3.99 ± 0.918
3.99AlaThr: 3.99 ± 0.618
3.406AlaVal: 3.406 ± 0.686
0.681AlaTrp: 0.681 ± 0.266
3.017AlaTyr: 3.017 ± 0.571
0.0AlaXaa: 0.0 ± 0.0
Cys
0.292CysAla: 0.292 ± 0.169
0.292CysCys: 0.292 ± 0.178
0.195CysAsp: 0.195 ± 0.145
0.487CysGlu: 0.487 ± 0.227
0.487CysPhe: 0.487 ± 0.217
0.292CysGly: 0.292 ± 0.212
0.195CysHis: 0.195 ± 0.148
0.097CysIle: 0.097 ± 0.103
0.779CysLys: 0.779 ± 0.294
0.195CysLeu: 0.195 ± 0.124
0.097CysMet: 0.097 ± 0.107
0.292CysAsn: 0.292 ± 0.152
0.292CysPro: 0.292 ± 0.157
0.389CysGln: 0.389 ± 0.193
0.195CysArg: 0.195 ± 0.148
0.097CysSer: 0.097 ± 0.097
0.389CysThr: 0.389 ± 0.199
0.195CysVal: 0.195 ± 0.124
0.195CysTrp: 0.195 ± 0.157
0.097CysTyr: 0.097 ± 0.089
0.0CysXaa: 0.0 ± 0.0
Asp
3.114AspAla: 3.114 ± 0.732
0.097AspCys: 0.097 ± 0.103
3.99AspAsp: 3.99 ± 0.746
5.06AspGlu: 5.06 ± 0.774
3.893AspPhe: 3.893 ± 0.674
5.158AspGly: 5.158 ± 1.061
0.681AspHis: 0.681 ± 0.219
4.866AspIle: 4.866 ± 0.683
6.423AspLys: 6.423 ± 1.067
4.671AspLeu: 4.671 ± 0.615
2.044AspMet: 2.044 ± 0.486
3.601AspAsn: 3.601 ± 0.657
1.654AspPro: 1.654 ± 0.414
1.849AspGln: 1.849 ± 0.445
2.044AspArg: 2.044 ± 0.398
3.698AspSer: 3.698 ± 0.548
3.211AspThr: 3.211 ± 0.442
3.893AspVal: 3.893 ± 0.602
1.46AspTrp: 1.46 ± 0.393
2.433AspTyr: 2.433 ± 0.491
0.0AspXaa: 0.0 ± 0.0
Glu
4.185GluAla: 4.185 ± 1.084
0.487GluCys: 0.487 ± 0.267
3.309GluAsp: 3.309 ± 0.616
4.866GluGlu: 4.866 ± 0.819
3.114GluPhe: 3.114 ± 0.428
2.822GluGly: 2.822 ± 0.572
1.362GluHis: 1.362 ± 0.405
5.742GluIle: 5.742 ± 0.752
7.007GluLys: 7.007 ± 0.998
7.201GluLeu: 7.201 ± 0.893
1.849GluMet: 1.849 ± 0.399
3.601GluAsn: 3.601 ± 0.707
1.557GluPro: 1.557 ± 0.452
3.406GluGln: 3.406 ± 0.621
2.044GluArg: 2.044 ± 0.544
3.795GluSer: 3.795 ± 0.608
3.309GluThr: 3.309 ± 0.509
3.99GluVal: 3.99 ± 0.738
1.752GluTrp: 1.752 ± 0.44
2.433GluTyr: 2.433 ± 0.445
0.0GluXaa: 0.0 ± 0.0
Phe
2.336PheAla: 2.336 ± 0.457
0.681PheCys: 0.681 ± 0.24
3.017PheAsp: 3.017 ± 0.7
2.627PheGlu: 2.627 ± 0.6
1.849PhePhe: 1.849 ± 0.412
2.53PheGly: 2.53 ± 0.595
0.389PheHis: 0.389 ± 0.176
2.627PheIle: 2.627 ± 0.476
3.211PheLys: 3.211 ± 0.587
3.017PheLeu: 3.017 ± 0.629
1.265PheMet: 1.265 ± 0.447
2.725PheAsn: 2.725 ± 0.466
1.557PhePro: 1.557 ± 0.422
1.07PheGln: 1.07 ± 0.365
1.362PheArg: 1.362 ± 0.399
2.919PheSer: 2.919 ± 0.652
2.141PheThr: 2.141 ± 0.543
2.433PheVal: 2.433 ± 0.588
0.584PheTrp: 0.584 ± 0.265
1.46PheTyr: 1.46 ± 0.407
0.0PheXaa: 0.0 ± 0.0
Gly
3.017GlyAla: 3.017 ± 0.924
0.0GlyCys: 0.0 ± 0.0
3.406GlyAsp: 3.406 ± 0.725
3.406GlyGlu: 3.406 ± 0.67
4.185GlyPhe: 4.185 ± 0.669
4.866GlyGly: 4.866 ± 0.972
1.07GlyHis: 1.07 ± 0.494
5.45GlyIle: 5.45 ± 0.948
4.574GlyLys: 4.574 ± 0.689
4.963GlyLeu: 4.963 ± 0.622
2.044GlyMet: 2.044 ± 0.432
3.893GlyAsn: 3.893 ± 0.625
1.07GlyPro: 1.07 ± 0.328
3.309GlyGln: 3.309 ± 0.567
2.433GlyArg: 2.433 ± 0.522
4.963GlySer: 4.963 ± 0.889
4.671GlyThr: 4.671 ± 1.183
3.893GlyVal: 3.893 ± 0.834
1.265GlyTrp: 1.265 ± 0.406
3.211GlyTyr: 3.211 ± 0.384
0.0GlyXaa: 0.0 ± 0.0
His
1.07HisAla: 1.07 ± 0.347
0.0HisCys: 0.0 ± 0.0
1.07HisAsp: 1.07 ± 0.315
1.362HisGlu: 1.362 ± 0.47
0.292HisPhe: 0.292 ± 0.196
0.973HisGly: 0.973 ± 0.356
0.195HisHis: 0.195 ± 0.13
0.681HisIle: 0.681 ± 0.287
1.168HisLys: 1.168 ± 0.282
0.779HisLeu: 0.779 ± 0.269
0.487HisMet: 0.487 ± 0.209
1.07HisAsn: 1.07 ± 0.318
0.389HisPro: 0.389 ± 0.182
0.973HisGln: 0.973 ± 0.253
0.292HisArg: 0.292 ± 0.2
1.168HisSer: 1.168 ± 0.247
0.389HisThr: 0.389 ± 0.193
0.389HisVal: 0.389 ± 0.212
0.0HisTrp: 0.0 ± 0.0
0.876HisTyr: 0.876 ± 0.294
0.0HisXaa: 0.0 ± 0.0
Ile
5.06IleAla: 5.06 ± 0.774
0.584IleCys: 0.584 ± 0.266
5.45IleAsp: 5.45 ± 0.661
3.99IleGlu: 3.99 ± 0.66
1.946IlePhe: 1.946 ± 0.618
4.671IleGly: 4.671 ± 1.011
0.487IleHis: 0.487 ± 0.219
5.158IleIle: 5.158 ± 0.82
5.839IleLys: 5.839 ± 0.808
4.185IleLeu: 4.185 ± 0.582
1.752IleMet: 1.752 ± 0.487
5.839IleAsn: 5.839 ± 0.776
1.946IlePro: 1.946 ± 0.406
2.725IleGln: 2.725 ± 0.536
3.017IleArg: 3.017 ± 0.613
4.963IleSer: 4.963 ± 0.88
3.601IleThr: 3.601 ± 0.649
4.671IleVal: 4.671 ± 0.527
0.292IleTrp: 0.292 ± 0.141
2.433IleTyr: 2.433 ± 0.618
0.0IleXaa: 0.0 ± 0.0
Lys
6.715LysAla: 6.715 ± 1.555
0.292LysCys: 0.292 ± 0.172
5.742LysAsp: 5.742 ± 0.93
5.644LysGlu: 5.644 ± 0.73
3.406LysPhe: 3.406 ± 0.518
5.839LysGly: 5.839 ± 0.959
2.044LysHis: 2.044 ± 0.519
5.839LysIle: 5.839 ± 0.661
7.299LysLys: 7.299 ± 1.26
6.812LysLeu: 6.812 ± 0.872
2.433LysMet: 2.433 ± 0.477
7.882LysAsn: 7.882 ± 0.913
3.017LysPro: 3.017 ± 0.662
4.671LysGln: 4.671 ± 0.938
3.893LysArg: 3.893 ± 0.736
5.255LysSer: 5.255 ± 0.757
5.45LysThr: 5.45 ± 0.532
4.574LysVal: 4.574 ± 0.726
0.876LysTrp: 0.876 ± 0.35
3.211LysTyr: 3.211 ± 0.572
0.0LysXaa: 0.0 ± 0.0
Leu
5.45LeuAla: 5.45 ± 0.724
0.681LeuCys: 0.681 ± 0.269
4.963LeuAsp: 4.963 ± 0.981
5.45LeuGlu: 5.45 ± 0.788
1.849LeuPhe: 1.849 ± 0.456
4.087LeuGly: 4.087 ± 0.686
0.487LeuHis: 0.487 ± 0.204
5.255LeuIle: 5.255 ± 0.575
7.591LeuLys: 7.591 ± 0.861
5.547LeuLeu: 5.547 ± 0.902
2.725LeuMet: 2.725 ± 0.531
6.228LeuAsn: 6.228 ± 0.73
2.141LeuPro: 2.141 ± 0.459
3.99LeuGln: 3.99 ± 0.634
2.627LeuArg: 2.627 ± 0.59
7.688LeuSer: 7.688 ± 1.137
4.379LeuThr: 4.379 ± 0.62
4.185LeuVal: 4.185 ± 0.625
0.681LeuTrp: 0.681 ± 0.298
2.433LeuTyr: 2.433 ± 0.498
0.0LeuXaa: 0.0 ± 0.0
Met
2.919MetAla: 2.919 ± 0.502
0.0MetCys: 0.0 ± 0.0
1.265MetAsp: 1.265 ± 0.44
1.362MetGlu: 1.362 ± 0.405
1.265MetPhe: 1.265 ± 0.364
1.362MetGly: 1.362 ± 0.479
0.097MetHis: 0.097 ± 0.105
1.362MetIle: 1.362 ± 0.339
3.698MetLys: 3.698 ± 0.716
1.654MetLeu: 1.654 ± 0.384
0.973MetMet: 0.973 ± 0.381
1.654MetAsn: 1.654 ± 0.489
0.389MetPro: 0.389 ± 0.204
1.557MetGln: 1.557 ± 0.322
1.46MetArg: 1.46 ± 0.362
2.238MetSer: 2.238 ± 0.552
2.725MetThr: 2.725 ± 0.576
0.973MetVal: 0.973 ± 0.244
0.292MetTrp: 0.292 ± 0.15
0.681MetTyr: 0.681 ± 0.34
0.0MetXaa: 0.0 ± 0.0
Asn
3.893AsnAla: 3.893 ± 0.538
0.389AsnCys: 0.389 ± 0.232
4.282AsnAsp: 4.282 ± 0.743
4.185AsnGlu: 4.185 ± 0.531
2.238AsnPhe: 2.238 ± 0.527
5.45AsnGly: 5.45 ± 1.208
1.168AsnHis: 1.168 ± 0.428
4.379AsnIle: 4.379 ± 0.859
5.547AsnLys: 5.547 ± 0.766
4.963AsnLeu: 4.963 ± 0.773
1.654AsnMet: 1.654 ± 0.499
3.99AsnAsn: 3.99 ± 0.678
1.849AsnPro: 1.849 ± 0.421
2.238AsnGln: 2.238 ± 0.489
2.822AsnArg: 2.822 ± 0.497
5.547AsnSer: 5.547 ± 0.886
3.503AsnThr: 3.503 ± 0.612
3.114AsnVal: 3.114 ± 0.491
0.195AsnTrp: 0.195 ± 0.138
2.238AsnTyr: 2.238 ± 0.521
0.0AsnXaa: 0.0 ± 0.0
Pro
1.654ProAla: 1.654 ± 0.515
0.097ProCys: 0.097 ± 0.106
1.265ProAsp: 1.265 ± 0.34
2.141ProGlu: 2.141 ± 0.455
1.168ProPhe: 1.168 ± 0.349
0.779ProGly: 0.779 ± 0.277
0.389ProHis: 0.389 ± 0.198
1.07ProIle: 1.07 ± 0.351
1.654ProLys: 1.654 ± 0.413
2.044ProLeu: 2.044 ± 0.412
0.681ProMet: 0.681 ± 0.265
1.654ProAsn: 1.654 ± 0.477
0.681ProPro: 0.681 ± 0.337
1.752ProGln: 1.752 ± 0.427
0.973ProArg: 0.973 ± 0.427
1.946ProSer: 1.946 ± 0.446
2.53ProThr: 2.53 ± 0.658
1.849ProVal: 1.849 ± 0.485
0.292ProTrp: 0.292 ± 0.137
0.681ProTyr: 0.681 ± 0.232
0.0ProXaa: 0.0 ± 0.0
Gln
3.698GlnAla: 3.698 ± 0.505
0.097GlnCys: 0.097 ± 0.089
2.336GlnAsp: 2.336 ± 0.499
3.211GlnGlu: 3.211 ± 0.538
1.46GlnPhe: 1.46 ± 0.337
3.795GlnGly: 3.795 ± 0.519
0.292GlnHis: 0.292 ± 0.183
2.919GlnIle: 2.919 ± 0.435
5.06GlnLys: 5.06 ± 1.325
4.185GlnLeu: 4.185 ± 0.628
1.362GlnMet: 1.362 ± 0.437
1.362GlnAsn: 1.362 ± 0.333
0.779GlnPro: 0.779 ± 0.189
2.725GlnGln: 2.725 ± 0.503
1.654GlnArg: 1.654 ± 0.417
3.698GlnSer: 3.698 ± 0.675
2.919GlnThr: 2.919 ± 0.432
2.433GlnVal: 2.433 ± 0.421
0.584GlnTrp: 0.584 ± 0.232
1.946GlnTyr: 1.946 ± 0.332
0.0GlnXaa: 0.0 ± 0.0
Arg
1.557ArgAla: 1.557 ± 0.423
0.389ArgCys: 0.389 ± 0.225
2.336ArgAsp: 2.336 ± 0.524
3.601ArgGlu: 3.601 ± 0.923
1.654ArgPhe: 1.654 ± 0.501
2.822ArgGly: 2.822 ± 0.683
0.487ArgHis: 0.487 ± 0.268
2.53ArgIle: 2.53 ± 0.62
2.822ArgLys: 2.822 ± 0.529
2.336ArgLeu: 2.336 ± 0.631
0.584ArgMet: 0.584 ± 0.212
2.627ArgAsn: 2.627 ± 0.569
1.265ArgPro: 1.265 ± 0.375
1.265ArgGln: 1.265 ± 0.314
0.973ArgArg: 0.973 ± 0.369
2.53ArgSer: 2.53 ± 0.575
1.946ArgThr: 1.946 ± 0.589
1.849ArgVal: 1.849 ± 0.351
0.779ArgTrp: 0.779 ± 0.247
1.849ArgTyr: 1.849 ± 0.426
0.0ArgXaa: 0.0 ± 0.0
Ser
3.698SerAla: 3.698 ± 1.04
0.195SerCys: 0.195 ± 0.14
5.255SerAsp: 5.255 ± 0.811
4.866SerGlu: 4.866 ± 0.745
2.822SerPhe: 2.822 ± 0.556
4.866SerGly: 4.866 ± 0.653
1.46SerHis: 1.46 ± 0.33
3.114SerIle: 3.114 ± 0.449
6.228SerLys: 6.228 ± 0.766
6.228SerLeu: 6.228 ± 0.783
1.557SerMet: 1.557 ± 0.333
4.768SerAsn: 4.768 ± 0.561
1.07SerPro: 1.07 ± 0.402
3.601SerGln: 3.601 ± 0.538
2.627SerArg: 2.627 ± 0.516
4.768SerSer: 4.768 ± 0.583
4.574SerThr: 4.574 ± 0.513
5.06SerVal: 5.06 ± 0.625
1.168SerTrp: 1.168 ± 0.283
2.433SerTyr: 2.433 ± 0.463
0.0SerXaa: 0.0 ± 0.0
Thr
4.574ThrAla: 4.574 ± 0.775
0.292ThrCys: 0.292 ± 0.196
3.309ThrAsp: 3.309 ± 0.446
3.503ThrGlu: 3.503 ± 0.63
1.849ThrPhe: 1.849 ± 0.439
5.158ThrGly: 5.158 ± 0.943
0.681ThrHis: 0.681 ± 0.186
4.768ThrIle: 4.768 ± 0.617
5.255ThrLys: 5.255 ± 0.88
5.158ThrLeu: 5.158 ± 1.024
1.362ThrMet: 1.362 ± 0.388
3.503ThrAsn: 3.503 ± 0.851
1.46ThrPro: 1.46 ± 0.453
1.946ThrGln: 1.946 ± 0.469
2.141ThrArg: 2.141 ± 0.493
3.601ThrSer: 3.601 ± 0.689
4.866ThrThr: 4.866 ± 1.168
5.839ThrVal: 5.839 ± 0.891
0.487ThrTrp: 0.487 ± 0.294
1.849ThrTyr: 1.849 ± 0.431
0.0ThrXaa: 0.0 ± 0.0
Val
4.379ValAla: 4.379 ± 0.991
0.097ValCys: 0.097 ± 0.096
4.379ValAsp: 4.379 ± 0.612
4.282ValGlu: 4.282 ± 0.559
2.238ValPhe: 2.238 ± 0.548
2.627ValGly: 2.627 ± 0.402
0.487ValHis: 0.487 ± 0.184
4.087ValIle: 4.087 ± 0.544
5.839ValLys: 5.839 ± 0.75
4.963ValLeu: 4.963 ± 0.555
1.557ValMet: 1.557 ± 0.425
3.503ValAsn: 3.503 ± 0.786
2.044ValPro: 2.044 ± 0.438
2.141ValGln: 2.141 ± 0.452
1.752ValArg: 1.752 ± 0.484
3.795ValSer: 3.795 ± 0.578
4.282ValThr: 4.282 ± 0.711
3.795ValVal: 3.795 ± 0.478
0.876ValTrp: 0.876 ± 0.279
1.46ValTyr: 1.46 ± 0.326
0.0ValXaa: 0.0 ± 0.0
Trp
0.681TrpAla: 0.681 ± 0.203
0.097TrpCys: 0.097 ± 0.1
0.389TrpAsp: 0.389 ± 0.278
0.584TrpGlu: 0.584 ± 0.278
0.389TrpPhe: 0.389 ± 0.196
0.584TrpGly: 0.584 ± 0.197
0.292TrpHis: 0.292 ± 0.16
1.362TrpIle: 1.362 ± 0.418
1.265TrpLys: 1.265 ± 0.383
1.557TrpLeu: 1.557 ± 0.327
0.195TrpMet: 0.195 ± 0.165
0.681TrpAsn: 0.681 ± 0.361
0.0TrpPro: 0.0 ± 0.0
0.779TrpGln: 0.779 ± 0.272
0.779TrpArg: 0.779 ± 0.309
0.779TrpSer: 0.779 ± 0.307
0.973TrpThr: 0.973 ± 0.388
0.973TrpVal: 0.973 ± 0.316
0.292TrpTrp: 0.292 ± 0.167
0.487TrpTyr: 0.487 ± 0.235
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.141TyrAla: 2.141 ± 0.353
0.584TyrCys: 0.584 ± 0.301
3.114TyrAsp: 3.114 ± 0.54
2.238TyrGlu: 2.238 ± 0.416
1.654TyrPhe: 1.654 ± 0.426
2.433TyrGly: 2.433 ± 0.437
0.487TyrHis: 0.487 ± 0.23
2.044TyrIle: 2.044 ± 0.441
4.574TyrLys: 4.574 ± 0.78
3.211TyrLeu: 3.211 ± 0.667
0.973TyrMet: 0.973 ± 0.321
1.265TyrAsn: 1.265 ± 0.319
0.779TyrPro: 0.779 ± 0.335
2.53TyrGln: 2.53 ± 0.553
1.07TyrArg: 1.07 ± 0.351
2.919TyrSer: 2.919 ± 0.485
1.752TyrThr: 1.752 ± 0.423
1.07TyrVal: 1.07 ± 0.304
0.389TyrTrp: 0.389 ± 0.213
1.07TyrTyr: 1.07 ± 0.297
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (10277 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski