Athene cunicularia (Burrowing owl) (Speotyto cunicularia)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria;

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22627 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A663N5P3|A0A663N5P3_ATHCN Myosin heavy chain 11 OS=Athene cunicularia OX=194338 GN=MYH11 PE=3 SV=1
MM1 pKa = 6.93GTEE4 pKa = 4.24SLSPEE9 pKa = 4.26GPALVPRR16 pKa = 11.84GYY18 pKa = 11.25NKK20 pKa = 10.12LHH22 pKa = 6.27SVNLQHH28 pKa = 6.94AHH30 pKa = 5.27VADD33 pKa = 4.47GQSHH37 pKa = 6.22SIIVRR42 pKa = 11.84LSGLRR47 pKa = 11.84GDD49 pKa = 3.9MLSVEE54 pKa = 5.06LYY56 pKa = 10.52VDD58 pKa = 4.55CKK60 pKa = 11.21QMDD63 pKa = 3.79SSVGLPEE70 pKa = 4.7LSEE73 pKa = 4.13IPLAEE78 pKa = 4.06VEE80 pKa = 4.5SIEE83 pKa = 4.74VRR85 pKa = 11.84TGQKK89 pKa = 10.77GFVEE93 pKa = 4.33SMKK96 pKa = 10.78LILGGSMSRR105 pKa = 11.84VGALSEE111 pKa = 4.81CPFQGDD117 pKa = 3.71EE118 pKa = 4.7SIHH121 pKa = 5.77SAGNGTAAPCTEE133 pKa = 3.67QGQRR137 pKa = 11.84GGLTGSPVHH146 pKa = 5.57ATARR150 pKa = 11.84SAVSAGEE157 pKa = 3.98APLPPRR163 pKa = 11.84GAKK166 pKa = 9.22GRR168 pKa = 11.84PASPPPWPSLGFHH181 pKa = 6.0EE182 pKa = 5.12HH183 pKa = 7.6RR184 pKa = 11.84SRR186 pKa = 11.84CNPNPCFSGVDD197 pKa = 3.37CMEE200 pKa = 4.13TYY202 pKa = 10.02EE203 pKa = 4.17YY204 pKa = 10.08PGYY207 pKa = 10.36RR208 pKa = 11.84CGPCPPGLEE217 pKa = 4.47GNGTSCADD225 pKa = 3.4INEE228 pKa = 4.28CAYY231 pKa = 10.69ANPCFPGSKK240 pKa = 9.86CINTAPGFRR249 pKa = 11.84CEE251 pKa = 4.01PCPRR255 pKa = 11.84GYY257 pKa = 10.53RR258 pKa = 11.84GNTVSGVGVDD268 pKa = 3.41YY269 pKa = 11.37AKK271 pKa = 10.72ASKK274 pKa = 9.68QVCTDD279 pKa = 2.78IDD281 pKa = 3.7EE282 pKa = 5.35CNDD285 pKa = 3.58GNNGGCDD292 pKa = 3.53PNSICTNTLGSYY304 pKa = 10.15KK305 pKa = 10.38CGPCKK310 pKa = 10.49SGFVGNQTSGCIPQKK325 pKa = 10.52SCSTPTSNPCDD336 pKa = 3.41INGFCMFEE344 pKa = 4.27RR345 pKa = 11.84NGEE348 pKa = 3.95ISCACNVGWAGNGNVCGQDD367 pKa = 3.15TDD369 pKa = 4.23LDD371 pKa = 4.44GYY373 pKa = 9.92PDD375 pKa = 4.1EE376 pKa = 5.31PLPCIDD382 pKa = 4.25NNKK385 pKa = 9.35HH386 pKa = 6.21CKK388 pKa = 9.14QDD390 pKa = 3.44NCRR393 pKa = 11.84LTPNSGQEE401 pKa = 3.95DD402 pKa = 4.26ADD404 pKa = 3.62NDD406 pKa = 5.11GIGDD410 pKa = 3.82QCDD413 pKa = 3.57DD414 pKa = 4.52DD415 pKa = 6.87ADD417 pKa = 4.09GDD419 pKa = 4.3GIKK422 pKa = 10.54NVEE425 pKa = 3.95VDD427 pKa = 3.34NCRR430 pKa = 11.84LFPNKK435 pKa = 9.91DD436 pKa = 3.46QQNSDD441 pKa = 3.11TDD443 pKa = 4.03SFGDD447 pKa = 4.2ACDD450 pKa = 3.62NCPNVPNNDD459 pKa = 3.4QRR461 pKa = 11.84DD462 pKa = 3.62TDD464 pKa = 4.31SNGEE468 pKa = 3.91GDD470 pKa = 4.42ACDD473 pKa = 3.98NDD475 pKa = 3.39IDD477 pKa = 5.15GDD479 pKa = 4.46GIPNMLDD486 pKa = 2.96NCPRR490 pKa = 11.84VPNPLQTDD498 pKa = 3.4RR499 pKa = 11.84DD500 pKa = 3.67EE501 pKa = 6.42DD502 pKa = 4.4GVGDD506 pKa = 4.27ACDD509 pKa = 3.66SCPEE513 pKa = 4.12MSNPTQTDD521 pKa = 3.06MDD523 pKa = 4.69SDD525 pKa = 4.47LVGDD529 pKa = 3.68ICDD532 pKa = 3.85TNEE535 pKa = 4.14DD536 pKa = 3.74SDD538 pKa = 4.93GDD540 pKa = 3.91GHH542 pKa = 7.38QDD544 pKa = 3.18TKK546 pKa = 11.57DD547 pKa = 3.32NCAEE551 pKa = 4.25IPNSSQLDD559 pKa = 3.58SDD561 pKa = 4.01NDD563 pKa = 4.11GLGDD567 pKa = 4.98DD568 pKa = 5.48CDD570 pKa = 4.94NDD572 pKa = 4.37DD573 pKa = 5.42DD574 pKa = 5.52NDD576 pKa = 5.09GIPDD580 pKa = 4.02YY581 pKa = 11.2VPPGPDD587 pKa = 2.98NCRR590 pKa = 11.84LIPNPNQKK598 pKa = 10.66DD599 pKa = 3.36SDD601 pKa = 4.17GNGVGDD607 pKa = 3.6VCEE610 pKa = 4.36EE611 pKa = 4.28DD612 pKa = 3.68FDD614 pKa = 5.16NDD616 pKa = 4.06TVVDD620 pKa = 3.93QLDD623 pKa = 3.73VCPEE627 pKa = 3.98SAEE630 pKa = 4.05VTLTDD635 pKa = 3.82FRR637 pKa = 11.84AYY639 pKa = 8.36QTVILDD645 pKa = 3.91PEE647 pKa = 5.2GDD649 pKa = 3.69AQIDD653 pKa = 4.13PNWVVLNQGMEE664 pKa = 4.11IVQTMNSDD672 pKa = 3.19PGLAVGYY679 pKa = 7.31TAFNGVDD686 pKa = 3.99FEE688 pKa = 4.97GTFHH692 pKa = 6.81VNTVTDD698 pKa = 3.57DD699 pKa = 4.08DD700 pKa = 4.34YY701 pKa = 12.06AGFIFSYY708 pKa = 10.41QDD710 pKa = 2.92SASFYY715 pKa = 10.36VVMWKK720 pKa = 7.78QTEE723 pKa = 3.86QTYY726 pKa = 8.13WQATPFRR733 pKa = 11.84AVAEE737 pKa = 4.58PGLQQ741 pKa = 3.16

Molecular weight: 78.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A663M9F8|A0A663M9F8_ATHCN T-complex protein 1 subunit gamma OS=Athene cunicularia OX=194338 PE=3 SV=1
II1 pKa = 7.08PRR3 pKa = 11.84PRR5 pKa = 11.84GAGSPRR11 pKa = 11.84PRR13 pKa = 11.84GAGSPRR19 pKa = 11.84PRR21 pKa = 11.84GAGSPRR27 pKa = 11.84PRR29 pKa = 11.84GAGSPRR35 pKa = 11.84PRR37 pKa = 11.84GAGSPRR43 pKa = 11.84PRR45 pKa = 11.84GAGSPRR51 pKa = 11.84PRR53 pKa = 11.84GAGSPRR59 pKa = 11.84PRR61 pKa = 11.84GAGSPRR67 pKa = 11.84PRR69 pKa = 11.84GAGSPRR75 pKa = 11.84PRR77 pKa = 11.84GAGSPRR83 pKa = 11.84PRR85 pKa = 11.84GAGSPRR91 pKa = 11.84PRR93 pKa = 11.84GAGSPRR99 pKa = 11.84PRR101 pKa = 11.84GAGSPRR107 pKa = 11.84PRR109 pKa = 11.84GAGSPWPRR117 pKa = 11.84GAGSPWPRR125 pKa = 11.84GAGSVTSRR133 pKa = 11.84APRR136 pKa = 11.84GTDD139 pKa = 2.94HH140 pKa = 7.27AGASAGEE147 pKa = 3.89RR148 pKa = 11.84EE149 pKa = 4.85GWRR152 pKa = 11.84EE153 pKa = 3.84HH154 pKa = 6.56PSWKK158 pKa = 10.09AAAPLHH164 pKa = 5.97SVLPAGPRR172 pKa = 11.84PAPCC176 pKa = 4.62

Molecular weight: 17.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13825

8802

22627

15598483

16

8698

689.4

77.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.412 ± 0.011

2.281 ± 0.013

5.053 ± 0.011

7.21 ± 0.023

3.82 ± 0.012

5.944 ± 0.018

2.536 ± 0.007

4.937 ± 0.012

6.239 ± 0.015

9.729 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.255 ± 0.006

4.131 ± 0.01

5.357 ± 0.019

4.746 ± 0.016

5.18 ± 0.011

8.329 ± 0.019

5.45 ± 0.01

6.235 ± 0.011

1.193 ± 0.005

2.889 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski