Amino acid dipepetide frequency for Athene cunicularia (Burrowing owl) (Speotyto cunicularia)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.776AlaAla: 5.776 ± 0.034	1.338AlaCys: 1.338 ± 0.012	3.048AlaAsp: 3.048 ± 0.015	4.672AlaGlu: 4.672 ± 0.025	2.478AlaPhe: 2.478 ± 0.012	4.079AlaGly: 4.079 ± 0.023	1.342AlaHis: 1.342 ± 0.01	2.962AlaIle: 2.962 ± 0.015	3.639AlaLys: 3.639 ± 0.018	6.446AlaLeu: 6.446 ± 0.027	1.482AlaMet: 1.482 ± 0.011	2.252AlaAsn: 2.252 ± 0.013	3.039AlaPro: 3.039 ± 0.021	2.805AlaGln: 2.805 ± 0.019	3.02AlaArg: 3.02 ± 0.015	5.271AlaSer: 5.271 ± 0.023	3.356AlaThr: 3.356 ± 0.018	4.827AlaVal: 4.827 ± 0.02	0.715AlaTrp: 0.715 ± 0.007	1.565AlaTyr: 1.565 ± 0.011	0.005AlaXaa: 0.005 ± 0.001
Cys	1.222CysAla: 1.222 ± 0.013	0.634CysCys: 0.634 ± 0.008	1.105CysAsp: 1.105 ± 0.014	1.346CysGlu: 1.346 ± 0.015	0.953CysPhe: 0.953 ± 0.008	1.476CysGly: 1.476 ± 0.016	0.672CysHis: 0.672 ± 0.008	1.176CysIle: 1.176 ± 0.011	1.3CysLys: 1.3 ± 0.013	2.265CysLeu: 2.265 ± 0.015	0.468CysMet: 0.468 ± 0.005	0.914CysAsn: 0.914 ± 0.011	1.291CysPro: 1.291 ± 0.014	1.09CysGln: 1.09 ± 0.013	1.247CysArg: 1.247 ± 0.01	2.109CysSer: 2.109 ± 0.017	1.222CysThr: 1.222 ± 0.011	1.376CysVal: 1.376 ± 0.012	0.295CysTrp: 0.295 ± 0.005	0.652CysTyr: 0.652 ± 0.007	0.001CysXaa: 0.001 ± 0.0
Asp	2.885AspAla: 2.885 ± 0.015	1.147AspCys: 1.147 ± 0.012	2.849AspAsp: 2.849 ± 0.021	3.701AspGlu: 3.701 ± 0.019	2.254AspPhe: 2.254 ± 0.015	3.365AspGly: 3.365 ± 0.021	1.167AspHis: 1.167 ± 0.009	2.953AspIle: 2.953 ± 0.017	2.777AspLys: 2.777 ± 0.014	5.04AspLeu: 5.04 ± 0.02	1.157AspMet: 1.157 ± 0.008	1.999AspAsn: 1.999 ± 0.015	2.675AspPro: 2.675 ± 0.015	1.866AspGln: 1.866 ± 0.011	2.355AspArg: 2.355 ± 0.016	4.206AspSer: 4.206 ± 0.022	2.625AspThr: 2.625 ± 0.014	3.251AspVal: 3.251 ± 0.02	0.637AspTrp: 0.637 ± 0.007	1.619AspTyr: 1.619 ± 0.011	0.001AspXaa: 0.001 ± 0.0
Glu	4.641GluAla: 4.641 ± 0.021	1.348GluCys: 1.348 ± 0.016	4.543GluAsp: 4.543 ± 0.021	7.868GluGlu: 7.868 ± 0.054	2.159GluPhe: 2.159 ± 0.014	4.03GluGly: 4.03 ± 0.02	1.587GluHis: 1.587 ± 0.011	3.595GluIle: 3.595 ± 0.02	5.695GluLys: 5.695 ± 0.032	6.428GluLeu: 6.428 ± 0.044	1.831GluMet: 1.831 ± 0.012	3.481GluAsn: 3.481 ± 0.018	2.656GluPro: 2.656 ± 0.016	3.301GluGln: 3.301 ± 0.024	3.872GluArg: 3.872 ± 0.025	4.552GluSer: 4.552 ± 0.024	3.638GluThr: 3.638 ± 0.018	4.323GluVal: 4.323 ± 0.018	0.736GluTrp: 0.736 ± 0.007	1.814GluTyr: 1.814 ± 0.01	0.002GluXaa: 0.002 ± 0.0
Phe	2.109PheAla: 2.109 ± 0.013	1.002PheCys: 1.002 ± 0.009	1.812PheAsp: 1.812 ± 0.011	2.153PheGlu: 2.153 ± 0.015	1.874PhePhe: 1.874 ± 0.015	2.237PheGly: 2.237 ± 0.018	1.076PheHis: 1.076 ± 0.008	2.083PheIle: 2.083 ± 0.014	1.932PheLys: 1.932 ± 0.013	4.2PheLeu: 4.2 ± 0.023	0.815PheMet: 0.815 ± 0.009	1.492PheAsn: 1.492 ± 0.009	1.933PhePro: 1.933 ± 0.013	1.802PheGln: 1.802 ± 0.012	1.88PheArg: 1.88 ± 0.012	3.48PheSer: 3.48 ± 0.016	2.187PheThr: 2.187 ± 0.014	2.316PheVal: 2.316 ± 0.014	0.51PheTrp: 0.51 ± 0.006	1.308PheTyr: 1.308 ± 0.009	0.001PheXaa: 0.001 ± 0.0
Gly	3.59GlyAla: 3.59 ± 0.02	1.259GlyCys: 1.259 ± 0.014	3.022GlyAsp: 3.022 ± 0.017	3.833GlyGlu: 3.833 ± 0.018	2.422GlyPhe: 2.422 ± 0.018	4.121GlyGly: 4.121 ± 0.033	1.552GlyHis: 1.552 ± 0.014	3.022GlyIle: 3.022 ± 0.017	3.903GlyLys: 3.903 ± 0.019	5.065GlyLeu: 5.065 ± 0.022	1.386GlyMet: 1.386 ± 0.012	2.632GlyAsn: 2.632 ± 0.016	2.785GlyPro: 2.785 ± 0.029	2.589GlyGln: 2.589 ± 0.018	3.273GlyArg: 3.273 ± 0.019	5.271GlySer: 5.271 ± 0.024	3.573GlyThr: 3.573 ± 0.019	3.443GlyVal: 3.443 ± 0.016	0.828GlyTrp: 0.828 ± 0.009	1.869GlyTyr: 1.869 ± 0.016	0.006GlyXaa: 0.006 ± 0.001
His	1.329HisAla: 1.329 ± 0.01	0.742HisCys: 0.742 ± 0.008	0.962HisAsp: 0.962 ± 0.008	1.389HisGlu: 1.389 ± 0.009	1.096HisPhe: 1.096 ± 0.008	1.493HisGly: 1.493 ± 0.012	0.873HisHis: 0.873 ± 0.009	1.361HisIle: 1.361 ± 0.011	1.335HisLys: 1.335 ± 0.011	2.809HisLeu: 2.809 ± 0.016	0.588HisMet: 0.588 ± 0.006	0.974HisAsn: 0.974 ± 0.009	1.505HisPro: 1.505 ± 0.014	1.192HisGln: 1.192 ± 0.01	1.445HisArg: 1.445 ± 0.012	2.22HisSer: 2.22 ± 0.015	1.317HisThr: 1.317 ± 0.01	1.522HisVal: 1.522 ± 0.011	0.337HisTrp: 0.337 ± 0.005	0.868HisTyr: 0.868 ± 0.008	0.001HisXaa: 0.001 ± 0.0
Ile	2.979IleAla: 2.979 ± 0.016	1.196IleCys: 1.196 ± 0.01	2.349IleAsp: 2.349 ± 0.016	2.952IleGlu: 2.952 ± 0.021	2.146IlePhe: 2.146 ± 0.016	2.451IleGly: 2.451 ± 0.016	1.374IleHis: 1.374 ± 0.01	2.743IleIle: 2.743 ± 0.018	3.041IleLys: 3.041 ± 0.016	4.952IleLeu: 4.952 ± 0.024	1.092IleMet: 1.092 ± 0.01	2.252IleAsn: 2.252 ± 0.014	2.79IlePro: 2.79 ± 0.015	2.493IleGln: 2.493 ± 0.015	2.52IleArg: 2.52 ± 0.013	4.138IleSer: 4.138 ± 0.018	2.896IleThr: 2.896 ± 0.019	2.883IleVal: 2.883 ± 0.017	0.548IleTrp: 0.548 ± 0.006	1.572IleTyr: 1.572 ± 0.011	0.0IleXaa: 0.0 ± 0.0
Lys	3.957LysAla: 3.957 ± 0.021	1.222LysCys: 1.222 ± 0.013	3.454LysAsp: 3.454 ± 0.018	5.512LysGlu: 5.512 ± 0.036	1.882LysPhe: 1.882 ± 0.013	3.313LysGly: 3.313 ± 0.021	1.532LysHis: 1.532 ± 0.011	3.145LysIle: 3.145 ± 0.017	5.124LysLys: 5.124 ± 0.033	5.705LysLeu: 5.705 ± 0.027	1.554LysMet: 1.554 ± 0.01	2.772LysAsn: 2.772 ± 0.015	3.014LysPro: 3.014 ± 0.02	2.968LysGln: 2.968 ± 0.02	3.318LysArg: 3.318 ± 0.021	4.267LysSer: 4.267 ± 0.021	3.394LysThr: 3.394 ± 0.016	3.765LysVal: 3.765 ± 0.017	0.678LysTrp: 0.678 ± 0.007	1.811LysTyr: 1.811 ± 0.012	0.002LysXaa: 0.002 ± 0.0
Leu	6.181LeuAla: 6.181 ± 0.025	2.224LeuCys: 2.224 ± 0.012	4.82LeuAsp: 4.82 ± 0.022	7.108LeuGlu: 7.108 ± 0.04	3.603LeuPhe: 3.603 ± 0.023	5.123LeuGly: 5.123 ± 0.023	2.734LeuHis: 2.734 ± 0.014	4.272LeuIle: 4.272 ± 0.023	6.323LeuLys: 6.323 ± 0.033	10.289LeuLeu: 10.289 ± 0.047	2.083LeuMet: 2.083 ± 0.013	3.975LeuAsn: 3.975 ± 0.018	5.425LeuPro: 5.425 ± 0.025	5.765LeuGln: 5.765 ± 0.034	5.17LeuArg: 5.17 ± 0.023	7.947LeuSer: 7.947 ± 0.026	5.094LeuThr: 5.094 ± 0.021	5.336LeuVal: 5.336 ± 0.024	1.09LeuTrp: 1.09 ± 0.009	2.72LeuTyr: 2.72 ± 0.016	0.004LeuXaa: 0.004 ± 0.0
Met	1.633MetAla: 1.633 ± 0.012	0.459MetCys: 0.459 ± 0.006	1.314MetAsp: 1.314 ± 0.009	1.92MetGlu: 1.92 ± 0.012	0.847MetPhe: 0.847 ± 0.008	1.258MetGly: 1.258 ± 0.012	0.529MetHis: 0.529 ± 0.007	0.967MetIle: 0.967 ± 0.008	1.609MetLys: 1.609 ± 0.011	2.106MetLeu: 2.106 ± 0.013	0.607MetMet: 0.607 ± 0.008	1.009MetAsn: 1.009 ± 0.009	1.063MetPro: 1.063 ± 0.011	1.058MetGln: 1.058 ± 0.011	1.038MetArg: 1.038 ± 0.009	1.581MetSer: 1.581 ± 0.011	1.15MetThr: 1.15 ± 0.01	1.478MetVal: 1.478 ± 0.01	0.256MetTrp: 0.256 ± 0.004	0.663MetTyr: 0.663 ± 0.007	0.0MetXaa: 0.0 ± 0.0
Asn	2.372AsnAla: 2.372 ± 0.015	0.999AsnCys: 0.999 ± 0.012	1.779AsnAsp: 1.779 ± 0.012	2.594AsnGlu: 2.594 ± 0.017	1.647AsnPhe: 1.647 ± 0.012	2.771AsnGly: 2.771 ± 0.021	1.006AsnHis: 1.006 ± 0.009	2.531AsnIle: 2.531 ± 0.013	2.601AsnLys: 2.601 ± 0.014	4.085AsnLeu: 4.085 ± 0.017	1.008AsnMet: 1.008 ± 0.009	1.847AsnAsn: 1.847 ± 0.014	2.246AsnPro: 2.246 ± 0.015	1.79AsnGln: 1.79 ± 0.011	2.0AsnArg: 2.0 ± 0.013	3.477AsnSer: 3.477 ± 0.016	2.288AsnThr: 2.288 ± 0.013	2.508AsnVal: 2.508 ± 0.015	0.484AsnTrp: 0.484 ± 0.006	1.279AsnTyr: 1.279 ± 0.011	0.001AsnXaa: 0.001 ± 0.0
Pro	3.884ProAla: 3.884 ± 0.024	1.136ProCys: 1.136 ± 0.013	2.622ProAsp: 2.622 ± 0.016	3.863ProGlu: 3.863 ± 0.018	1.853ProPhe: 1.853 ± 0.013	3.787ProGly: 3.787 ± 0.039	1.281ProHis: 1.281 ± 0.013	1.94ProIle: 1.94 ± 0.014	2.685ProLys: 2.685 ± 0.017	4.754ProLeu: 4.754 ± 0.024	0.956ProMet: 0.956 ± 0.009	1.88ProAsn: 1.88 ± 0.012	4.457ProPro: 4.457 ± 0.041	2.415ProGln: 2.415 ± 0.019	2.561ProArg: 2.561 ± 0.017	5.036ProSer: 5.036 ± 0.031	2.725ProThr: 2.725 ± 0.016	3.732ProVal: 3.732 ± 0.022	0.582ProTrp: 0.582 ± 0.006	1.41ProTyr: 1.41 ± 0.011	0.007ProXaa: 0.007 ± 0.001
Gln	3.152GlnAla: 3.152 ± 0.019	1.02GlnCys: 1.02 ± 0.011	2.262GlnAsp: 2.262 ± 0.012	3.762GlnGlu: 3.762 ± 0.026	1.463GlnPhe: 1.463 ± 0.01	2.559GlnGly: 2.559 ± 0.014	1.332GlnHis: 1.332 ± 0.01	2.247GlnIle: 2.247 ± 0.015	3.142GlnLys: 3.142 ± 0.02	4.711GlnLeu: 4.711 ± 0.029	1.169GlnMet: 1.169 ± 0.01	2.062GlnAsn: 2.062 ± 0.016	2.442GlnPro: 2.442 ± 0.02	3.152GlnGln: 3.152 ± 0.034	2.691GlnArg: 2.691 ± 0.015	3.268GlnSer: 3.268 ± 0.018	2.4GlnThr: 2.4 ± 0.015	2.781GlnVal: 2.781 ± 0.017	0.563GlnTrp: 0.563 ± 0.006	1.282GlnTyr: 1.282 ± 0.01	0.001GlnXaa: 0.001 ± 0.0
Arg	3.15ArgAla: 3.15 ± 0.016	1.178ArgCys: 1.178 ± 0.012	2.587ArgAsp: 2.587 ± 0.015	3.69ArgGlu: 3.69 ± 0.022	1.804ArgPhe: 1.804 ± 0.013	3.019ArgGly: 3.019 ± 0.018	1.39ArgHis: 1.39 ± 0.012	2.617ArgIle: 2.617 ± 0.013	3.62ArgLys: 3.62 ± 0.02	4.838ArgLeu: 4.838 ± 0.022	1.212ArgMet: 1.212 ± 0.01	2.227ArgAsn: 2.227 ± 0.014	2.483ArgPro: 2.483 ± 0.016	2.496ArgGln: 2.496 ± 0.017	3.647ArgArg: 3.647 ± 0.025	3.998ArgSer: 3.998 ± 0.027	2.713ArgThr: 2.713 ± 0.014	2.958ArgVal: 2.958 ± 0.016	0.645ArgTrp: 0.645 ± 0.007	1.521ArgTyr: 1.521 ± 0.01	0.004ArgXaa: 0.004 ± 0.0
Ser	5.171SerAla: 5.171 ± 0.024	1.918SerCys: 1.918 ± 0.016	4.086SerAsp: 4.086 ± 0.022	5.254SerGlu: 5.254 ± 0.026	3.137SerPhe: 3.137 ± 0.016	5.142SerGly: 5.142 ± 0.026	2.039SerHis: 2.039 ± 0.014	3.663SerIle: 3.663 ± 0.017	4.444SerLys: 4.444 ± 0.022	8.097SerLeu: 8.097 ± 0.029	1.637SerMet: 1.637 ± 0.01	3.077SerAsn: 3.077 ± 0.017	5.397SerPro: 5.397 ± 0.036	3.73SerGln: 3.73 ± 0.02	4.125SerArg: 4.125 ± 0.023	9.281SerSer: 9.281 ± 0.051	4.683SerThr: 4.683 ± 0.021	5.206SerVal: 5.206 ± 0.021	1.007SerTrp: 1.007 ± 0.009	2.186SerTyr: 2.186 ± 0.013	0.004SerXaa: 0.004 ± 0.0
Thr	3.854ThrAla: 3.854 ± 0.018	1.407ThrCys: 1.407 ± 0.017	2.786ThrAsp: 2.786 ± 0.017	3.816ThrGlu: 3.816 ± 0.019	2.175ThrPhe: 2.175 ± 0.013	3.512ThrGly: 3.512 ± 0.018	1.201ThrHis: 1.201 ± 0.01	2.596ThrIle: 2.596 ± 0.015	2.877ThrLys: 2.877 ± 0.017	5.23ThrLeu: 5.23 ± 0.022	1.114ThrMet: 1.114 ± 0.01	1.961ThrAsn: 1.961 ± 0.013	3.204ThrPro: 3.204 ± 0.021	2.216ThrGln: 2.216 ± 0.014	2.328ThrArg: 2.328 ± 0.012	4.774ThrSer: 4.774 ± 0.022	3.059ThrThr: 3.059 ± 0.021	4.155ThrVal: 4.155 ± 0.022	0.688ThrTrp: 0.688 ± 0.007	1.546ThrTyr: 1.546 ± 0.012	0.002ThrXaa: 0.002 ± 0.0
Val	4.045ValAla: 4.045 ± 0.02	1.574ValCys: 1.574 ± 0.013	3.042ValAsp: 3.042 ± 0.017	4.057ValGlu: 4.057 ± 0.02	2.576ValPhe: 2.576 ± 0.015	3.286ValGly: 3.286 ± 0.017	1.551ValHis: 1.551 ± 0.01	3.243ValIle: 3.243 ± 0.017	3.802ValLys: 3.802 ± 0.019	6.236ValLeu: 6.236 ± 0.027	1.395ValMet: 1.395 ± 0.01	2.638ValAsn: 2.638 ± 0.016	3.417ValPro: 3.417 ± 0.019	2.863ValGln: 2.863 ± 0.017	2.985ValArg: 2.985 ± 0.015	5.101ValSer: 5.101 ± 0.022	3.89ValThr: 3.89 ± 0.022	4.136ValVal: 4.136 ± 0.021	0.732ValTrp: 0.732 ± 0.008	1.773ValTyr: 1.773 ± 0.012	0.002ValXaa: 0.002 ± 0.0
Trp	0.666TrpAla: 0.666 ± 0.006	0.264TrpCys: 0.264 ± 0.005	0.651TrpAsp: 0.651 ± 0.007	0.804TrpGlu: 0.804 ± 0.007	0.448TrpPhe: 0.448 ± 0.005	0.679TrpGly: 0.679 ± 0.008	0.324TrpHis: 0.324 ± 0.005	0.643TrpIle: 0.643 ± 0.007	0.839TrpLys: 0.839 ± 0.007	1.218TrpLeu: 1.218 ± 0.011	0.326TrpMet: 0.326 ± 0.005	0.583TrpAsn: 0.583 ± 0.006	0.461TrpPro: 0.461 ± 0.006	0.569TrpGln: 0.569 ± 0.007	0.668TrpArg: 0.668 ± 0.006	0.906TrpSer: 0.906 ± 0.008	0.646TrpThr: 0.646 ± 0.008	0.667TrpVal: 0.667 ± 0.008	0.2TrpTrp: 0.2 ± 0.004	0.37TrpTyr: 0.37 ± 0.006	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.513TyrAla: 1.513 ± 0.01	0.743TyrCys: 0.743 ± 0.008	1.433TyrAsp: 1.433 ± 0.012	1.804TyrGlu: 1.804 ± 0.011	1.384TyrPhe: 1.384 ± 0.01	1.731TyrGly: 1.731 ± 0.013	0.796TyrHis: 0.796 ± 0.007	1.607TyrIle: 1.607 ± 0.01	1.701TyrLys: 1.701 ± 0.01	2.809TyrLeu: 2.809 ± 0.016	0.666TyrMet: 0.666 ± 0.007	1.286TyrAsn: 1.286 ± 0.011	1.28TyrPro: 1.28 ± 0.012	1.301TyrGln: 1.301 ± 0.012	1.65TyrArg: 1.65 ± 0.013	2.4TyrSer: 2.4 ± 0.015	1.642TyrThr: 1.642 ± 0.013	1.68TyrVal: 1.68 ± 0.013	0.402TyrTrp: 0.402 ± 0.007	1.065TyrTyr: 1.065 ± 0.009	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.006XaaAla: 0.006 ± 0.001	0.002XaaCys: 0.002 ± 0.0	0.001XaaAsp: 0.001 ± 0.0	0.002XaaGlu: 0.002 ± 0.0	0.002XaaPhe: 0.002 ± 0.0	0.009XaaGly: 0.009 ± 0.001	0.001XaaHis: 0.001 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.0	0.003XaaLeu: 0.003 ± 0.0	0.002XaaMet: 0.002 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.006XaaPro: 0.006 ± 0.001	0.001XaaGln: 0.001 ± 0.0	0.004XaaArg: 0.004 ± 0.001	0.003XaaSer: 0.003 ± 0.0	0.001XaaThr: 0.001 ± 0.0	0.002XaaVal: 0.002 ± 0.0	0.001XaaTrp: 0.001 ± 0.0	0.001XaaTyr: 0.001 ± 0.0	0.701XaaXaa: 0.701 ± 0.036

Statistics based on 22627 proteins (15598483 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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