uncultured phage_MedDCM-OCT-S42-C7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Sieqvirus; Sieqvirus S42C7

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6S4PG31|A0A6S4PG31_9CAUD Terminase large subunit OS=uncultured phage_MedDCM-OCT-S42-C7 OX=2741073 PE=3 SV=1
MM1 pKa = 7.47ANSFVRR7 pKa = 11.84YY8 pKa = 7.29TGNGSTTQYY17 pKa = 11.2AVNFTYY23 pKa = 10.41RR24 pKa = 11.84DD25 pKa = 3.44QADD28 pKa = 3.18ITVTINGVATTAFTYY43 pKa = 10.23NASGTQITFNTAPASASAIEE63 pKa = 4.04IRR65 pKa = 11.84RR66 pKa = 11.84TTSQTSRR73 pKa = 11.84LVDD76 pKa = 3.45YY77 pKa = 10.84AAGSVLTEE85 pKa = 4.12NDD87 pKa = 3.91LDD89 pKa = 3.99TDD91 pKa = 4.22SNQAFFMSQEE101 pKa = 4.56AIDD104 pKa = 4.32DD105 pKa = 4.19AGDD108 pKa = 3.85VIKK111 pKa = 10.87LDD113 pKa = 3.53NANFQWDD120 pKa = 4.02VQNKK124 pKa = 9.48RR125 pKa = 11.84LTNVADD131 pKa = 3.91PVNNTDD137 pKa = 3.4GVNKK141 pKa = 9.92QFISTNLPNITTVAGISGNVTTVAGISANVTSVASNSANINTVATNIANVNTVASDD197 pKa = 3.15IAKK200 pKa = 10.33VIVVANDD207 pKa = 3.31LNEE210 pKa = 4.25TVSEE214 pKa = 4.29IEE216 pKa = 3.98TAALDD221 pKa = 3.94LQEE224 pKa = 4.36TTSEE228 pKa = 3.89IDD230 pKa = 3.42TVSNNIANVNTVGTNIANVNTLAPISANITTVAGISANVTTVAGISSNVTSVANNSSNINSAVSNATNINTVAGNNSNITTVAGISSDD318 pKa = 3.51VTNVANIASDD328 pKa = 3.56VSAVEE333 pKa = 4.07NIKK336 pKa = 11.33ANVTTVAGISANVTTAATNNANITTVANAITNVNNVGGSIANVNSVASNLSGVNSFAEE394 pKa = 4.5RR395 pKa = 11.84YY396 pKa = 9.29RR397 pKa = 11.84IASSAPSSSNDD408 pKa = 3.15VGDD411 pKa = 4.72LYY413 pKa = 11.48FDD415 pKa = 3.53TTANEE420 pKa = 3.89LKK422 pKa = 10.59VYY424 pKa = 8.24KK425 pKa = 9.92TSGWAAAGSTVNGTSNRR442 pKa = 11.84FEE444 pKa = 4.16YY445 pKa = 10.09TATAGQTTFSGADD458 pKa = 3.32ANGSTLAYY466 pKa = 10.04DD467 pKa = 3.82AGFIDD472 pKa = 6.15LYY474 pKa = 11.47VNGIKK479 pKa = 10.32LANSDD484 pKa = 3.85FTATSGNSVVLASAAAVNDD503 pKa = 4.05IISVVAYY510 pKa = 7.92GTFQLANISIKK521 pKa = 10.87DD522 pKa = 3.73LTDD525 pKa = 3.36TPASFGTAGQALVMNTNANGLEE547 pKa = 3.99FANASSAEE555 pKa = 4.06VYY557 pKa = 10.54GFNLSDD563 pKa = 3.68TNSDD567 pKa = 4.2NINDD571 pKa = 4.15SLTVTTTNGGNDD583 pKa = 3.95NINASTYY590 pKa = 10.85AAFDD594 pKa = 3.91DD595 pKa = 4.06VVYY598 pKa = 10.57AATGFSWSLNSNGHH612 pKa = 7.24LIATII617 pKa = 3.7

Molecular weight: 63.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6S4PLQ2|A0A6S4PLQ2_9CAUD Putative carbohydrate binding domain containing protein OS=uncultured phage_MedDCM-OCT-S42-C7 OX=2741073 PE=4 SV=1
MM1 pKa = 7.78AKK3 pKa = 9.45TGLYY7 pKa = 10.87ANIHH11 pKa = 6.08KK12 pKa = 10.34KK13 pKa = 9.23RR14 pKa = 11.84ARR16 pKa = 11.84IKK18 pKa = 10.33AGSGEE23 pKa = 4.1KK24 pKa = 9.48MRR26 pKa = 11.84TAGTKK31 pKa = 9.73GRR33 pKa = 11.84PTAAQFKK40 pKa = 9.73RR41 pKa = 11.84AAKK44 pKa = 8.06TAKK47 pKa = 10.08SS48 pKa = 3.41

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

11222

33

837

249.4

27.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.352 ± 0.577

0.9 ± 0.174

5.997 ± 0.29

6.469 ± 0.53

3.796 ± 0.198

7.049 ± 1.091

1.729 ± 0.203

6.371 ± 0.194

7.886 ± 0.679

7.815 ± 0.528

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.522 ± 0.253

6.469 ± 0.554

2.958 ± 0.235

4.046 ± 0.282

3.93 ± 0.375

7.298 ± 0.561

6.879 ± 0.557

5.962 ± 0.384

1.15 ± 0.128

3.422 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski