Amino acid dipepetide frequency for uncultured phage_MedDCM-OCT-S42-C7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.367AlaAla: 4.367 ± 0.932
0.713AlaCys: 0.713 ± 0.28
4.812AlaAsp: 4.812 ± 0.503
4.902AlaGlu: 4.902 ± 0.819
2.584AlaPhe: 2.584 ± 0.575
5.347AlaGly: 5.347 ± 1.318
0.802AlaHis: 0.802 ± 0.221
4.991AlaIle: 4.991 ± 0.77
6.149AlaLys: 6.149 ± 0.715
4.812AlaLeu: 4.812 ± 0.615
2.406AlaMet: 2.406 ± 0.485
5.704AlaAsn: 5.704 ± 1.798
2.495AlaPro: 2.495 ± 0.578
2.852AlaGln: 2.852 ± 0.599
2.317AlaArg: 2.317 ± 0.524
6.149AlaSer: 6.149 ± 1.184
4.634AlaThr: 4.634 ± 0.85
3.921AlaVal: 3.921 ± 0.615
0.891AlaTrp: 0.891 ± 0.245
2.674AlaTyr: 2.674 ± 0.514
0.0AlaXaa: 0.0 ± 0.0
Cys
0.267CysAla: 0.267 ± 0.16
0.089CysCys: 0.089 ± 0.091
0.356CysAsp: 0.356 ± 0.195
0.267CysGlu: 0.267 ± 0.158
0.446CysPhe: 0.446 ± 0.206
0.713CysGly: 0.713 ± 0.271
0.535CysHis: 0.535 ± 0.198
0.624CysIle: 0.624 ± 0.269
1.069CysLys: 1.069 ± 0.263
1.069CysLeu: 1.069 ± 0.36
0.178CysMet: 0.178 ± 0.132
0.267CysAsn: 0.267 ± 0.171
0.356CysPro: 0.356 ± 0.183
0.624CysGln: 0.624 ± 0.21
0.535CysArg: 0.535 ± 0.201
0.713CysSer: 0.713 ± 0.264
0.267CysThr: 0.267 ± 0.149
0.446CysVal: 0.446 ± 0.178
0.089CysTrp: 0.089 ± 0.118
0.089CysTyr: 0.089 ± 0.1
0.0CysXaa: 0.0 ± 0.0
Asp
3.476AspAla: 3.476 ± 0.5
0.535AspCys: 0.535 ± 0.236
3.297AspAsp: 3.297 ± 0.597
4.189AspGlu: 4.189 ± 0.673
2.317AspPhe: 2.317 ± 0.464
3.832AspGly: 3.832 ± 0.65
0.891AspHis: 0.891 ± 0.326
4.278AspIle: 4.278 ± 0.6
5.08AspLys: 5.08 ± 0.802
6.595AspLeu: 6.595 ± 0.814
1.159AspMet: 1.159 ± 0.343
3.297AspAsn: 3.297 ± 0.49
1.871AspPro: 1.871 ± 0.436
1.871AspGln: 1.871 ± 0.398
1.693AspArg: 1.693 ± 0.425
4.099AspSer: 4.099 ± 0.735
4.189AspThr: 4.189 ± 0.734
4.189AspVal: 4.189 ± 0.525
0.802AspTrp: 0.802 ± 0.251
2.317AspTyr: 2.317 ± 0.494
0.0AspXaa: 0.0 ± 0.0
Glu
5.169GluAla: 5.169 ± 0.782
1.159GluCys: 1.159 ± 0.36
3.476GluAsp: 3.476 ± 0.636
4.634GluGlu: 4.634 ± 0.834
2.763GluPhe: 2.763 ± 0.513
3.297GluGly: 3.297 ± 0.678
1.604GluHis: 1.604 ± 0.391
4.634GluIle: 4.634 ± 0.61
5.614GluLys: 5.614 ± 1.048
4.812GluLeu: 4.812 ± 0.75
1.961GluMet: 1.961 ± 0.478
3.832GluAsn: 3.832 ± 0.647
1.961GluPro: 1.961 ± 0.657
2.941GluGln: 2.941 ± 0.696
3.476GluArg: 3.476 ± 0.623
2.852GluSer: 2.852 ± 0.545
4.189GluThr: 4.189 ± 0.728
3.03GluVal: 3.03 ± 0.632
0.624GluTrp: 0.624 ± 0.255
2.674GluTyr: 2.674 ± 0.456
0.0GluXaa: 0.0 ± 0.0
Phe
2.495PheAla: 2.495 ± 0.499
0.089PheCys: 0.089 ± 0.1
3.03PheAsp: 3.03 ± 0.774
3.03PheGlu: 3.03 ± 0.568
1.069PhePhe: 1.069 ± 0.334
2.495PheGly: 2.495 ± 0.5
0.535PheHis: 0.535 ± 0.22
2.139PheIle: 2.139 ± 0.526
2.584PheLys: 2.584 ± 0.535
3.119PheLeu: 3.119 ± 0.622
1.159PheMet: 1.159 ± 0.359
1.871PheAsn: 1.871 ± 0.438
1.159PhePro: 1.159 ± 0.253
1.782PheGln: 1.782 ± 0.413
1.515PheArg: 1.515 ± 0.309
2.763PheSer: 2.763 ± 0.428
2.852PheThr: 2.852 ± 0.584
2.763PheVal: 2.763 ± 0.396
0.446PheTrp: 0.446 ± 0.204
1.069PheTyr: 1.069 ± 0.312
0.0PheXaa: 0.0 ± 0.0
Gly
4.099GlyAla: 4.099 ± 1.225
0.267GlyCys: 0.267 ± 0.143
3.297GlyAsp: 3.297 ± 0.641
3.297GlyGlu: 3.297 ± 0.478
3.565GlyPhe: 3.565 ± 0.88
9.625GlyGly: 9.625 ± 5.844
1.871GlyHis: 1.871 ± 0.421
4.634GlyIle: 4.634 ± 0.632
4.812GlyLys: 4.812 ± 0.683
4.902GlyLeu: 4.902 ± 0.616
1.693GlyMet: 1.693 ± 0.383
4.456GlyAsn: 4.456 ± 0.728
0.089GlyPro: 0.089 ± 0.076
2.495GlyGln: 2.495 ± 0.425
2.941GlyArg: 2.941 ± 0.617
6.595GlySer: 6.595 ± 1.888
4.812GlyThr: 4.812 ± 1.061
4.812GlyVal: 4.812 ± 0.639
0.356GlyTrp: 0.356 ± 0.17
1.871GlyTyr: 1.871 ± 0.435
0.0GlyXaa: 0.0 ± 0.0
His
1.426HisAla: 1.426 ± 0.306
0.356HisCys: 0.356 ± 0.155
1.248HisAsp: 1.248 ± 0.401
0.535HisGlu: 0.535 ± 0.245
0.802HisPhe: 0.802 ± 0.26
1.515HisGly: 1.515 ± 0.373
0.356HisHis: 0.356 ± 0.189
1.159HisIle: 1.159 ± 0.321
0.446HisLys: 0.446 ± 0.183
2.139HisLeu: 2.139 ± 0.513
0.446HisMet: 0.446 ± 0.188
0.891HisAsn: 0.891 ± 0.256
0.267HisPro: 0.267 ± 0.154
0.446HisGln: 0.446 ± 0.291
0.713HisArg: 0.713 ± 0.281
1.069HisSer: 1.069 ± 0.328
1.069HisThr: 1.069 ± 0.293
0.802HisVal: 0.802 ± 0.237
0.624HisTrp: 0.624 ± 0.254
0.98HisTyr: 0.98 ± 0.254
0.0HisXaa: 0.0 ± 0.0
Ile
6.06IleAla: 6.06 ± 0.687
0.446IleCys: 0.446 ± 0.231
5.525IleAsp: 5.525 ± 0.638
4.189IleGlu: 4.189 ± 0.649
1.069IlePhe: 1.069 ± 0.338
3.743IleGly: 3.743 ± 0.613
0.98IleHis: 0.98 ± 0.313
3.832IleIle: 3.832 ± 0.604
4.812IleLys: 4.812 ± 0.788
4.634IleLeu: 4.634 ± 0.74
1.693IleMet: 1.693 ± 0.361
4.545IleAsn: 4.545 ± 0.85
2.406IlePro: 2.406 ± 0.574
2.05IleGln: 2.05 ± 0.307
2.584IleArg: 2.584 ± 0.559
5.436IleSer: 5.436 ± 0.812
4.723IleThr: 4.723 ± 0.728
3.208IleVal: 3.208 ± 0.575
0.267IleTrp: 0.267 ± 0.153
1.515IleTyr: 1.515 ± 0.391
0.0IleXaa: 0.0 ± 0.0
Lys
4.456LysAla: 4.456 ± 0.803
0.802LysCys: 0.802 ± 0.351
5.169LysAsp: 5.169 ± 0.737
6.506LysGlu: 6.506 ± 1.062
2.584LysPhe: 2.584 ± 0.545
4.099LysGly: 4.099 ± 0.644
1.515LysHis: 1.515 ± 0.35
5.08LysIle: 5.08 ± 0.909
6.595LysLys: 6.595 ± 0.984
6.951LysLeu: 6.951 ± 0.859
2.05LysMet: 2.05 ± 0.451
3.654LysAsn: 3.654 ± 0.633
3.03LysPro: 3.03 ± 0.625
2.941LysGln: 2.941 ± 0.602
4.545LysArg: 4.545 ± 0.829
3.921LysSer: 3.921 ± 0.564
6.06LysThr: 6.06 ± 0.885
5.08LysVal: 5.08 ± 0.766
0.802LysTrp: 0.802 ± 0.277
3.03LysTyr: 3.03 ± 0.56
0.0LysXaa: 0.0 ± 0.0
Leu
6.238LeuAla: 6.238 ± 0.934
0.624LeuCys: 0.624 ± 0.191
4.991LeuAsp: 4.991 ± 0.739
5.971LeuGlu: 5.971 ± 0.899
2.763LeuPhe: 2.763 ± 0.511
3.743LeuGly: 3.743 ± 0.644
0.98LeuHis: 0.98 ± 0.435
4.545LeuIle: 4.545 ± 0.627
8.021LeuLys: 8.021 ± 0.982
5.971LeuLeu: 5.971 ± 1.046
1.515LeuMet: 1.515 ± 0.328
5.793LeuAsn: 5.793 ± 0.623
3.387LeuPro: 3.387 ± 0.543
3.654LeuGln: 3.654 ± 0.563
3.297LeuArg: 3.297 ± 0.694
5.347LeuSer: 5.347 ± 0.648
4.278LeuThr: 4.278 ± 0.607
3.565LeuVal: 3.565 ± 0.601
0.624LeuTrp: 0.624 ± 0.216
2.852LeuTyr: 2.852 ± 0.546
0.0LeuXaa: 0.0 ± 0.0
Met
3.119MetAla: 3.119 ± 0.558
0.535MetCys: 0.535 ± 0.244
1.604MetAsp: 1.604 ± 0.357
1.069MetGlu: 1.069 ± 0.294
1.159MetPhe: 1.159 ± 0.368
1.069MetGly: 1.069 ± 0.335
0.267MetHis: 0.267 ± 0.138
1.426MetIle: 1.426 ± 0.411
1.782MetLys: 1.782 ± 0.424
1.871MetLeu: 1.871 ± 0.385
0.98MetMet: 0.98 ± 0.341
1.693MetAsn: 1.693 ± 0.455
0.98MetPro: 0.98 ± 0.286
0.713MetGln: 0.713 ± 0.299
1.337MetArg: 1.337 ± 0.392
2.584MetSer: 2.584 ± 0.397
1.337MetThr: 1.337 ± 0.36
0.891MetVal: 0.891 ± 0.23
0.267MetTrp: 0.267 ± 0.126
0.535MetTyr: 0.535 ± 0.207
0.0MetXaa: 0.0 ± 0.0
Asn
4.634AsnAla: 4.634 ± 1.121
0.356AsnCys: 0.356 ± 0.177
3.208AsnAsp: 3.208 ± 0.638
3.743AsnGlu: 3.743 ± 0.447
2.941AsnPhe: 2.941 ± 0.416
5.258AsnGly: 5.258 ± 1.019
1.693AsnHis: 1.693 ± 0.382
5.614AsnIle: 5.614 ± 1.228
4.634AsnLys: 4.634 ± 0.78
4.991AsnLeu: 4.991 ± 0.495
1.426AsnMet: 1.426 ± 0.355
3.476AsnAsn: 3.476 ± 0.783
1.961AsnPro: 1.961 ± 0.467
2.584AsnGln: 2.584 ± 0.425
2.406AsnArg: 2.406 ± 0.464
4.456AsnSer: 4.456 ± 0.879
3.565AsnThr: 3.565 ± 0.801
4.812AsnVal: 4.812 ± 1.12
0.624AsnTrp: 0.624 ± 0.286
2.317AsnTyr: 2.317 ± 0.441
0.0AsnXaa: 0.0 ± 0.0
Pro
0.98ProAla: 0.98 ± 0.295
0.624ProCys: 0.624 ± 0.212
1.693ProAsp: 1.693 ± 0.542
2.139ProGlu: 2.139 ± 0.375
1.248ProPhe: 1.248 ± 0.312
0.624ProGly: 0.624 ± 0.216
0.446ProHis: 0.446 ± 0.196
1.871ProIle: 1.871 ± 0.447
2.674ProLys: 2.674 ± 0.683
2.228ProLeu: 2.228 ± 0.421
0.802ProMet: 0.802 ± 0.251
2.852ProAsn: 2.852 ± 0.523
0.624ProPro: 0.624 ± 0.271
1.159ProGln: 1.159 ± 0.417
0.713ProArg: 0.713 ± 0.209
2.495ProSer: 2.495 ± 0.353
3.387ProThr: 3.387 ± 0.65
1.515ProVal: 1.515 ± 0.422
0.356ProTrp: 0.356 ± 0.221
1.159ProTyr: 1.159 ± 0.41
0.0ProXaa: 0.0 ± 0.0
Gln
3.297GlnAla: 3.297 ± 0.627
0.178GlnCys: 0.178 ± 0.123
1.871GlnAsp: 1.871 ± 0.472
3.297GlnGlu: 3.297 ± 0.566
1.604GlnPhe: 1.604 ± 0.362
1.782GlnGly: 1.782 ± 0.492
0.446GlnHis: 0.446 ± 0.222
2.941GlnIle: 2.941 ± 0.423
2.763GlnLys: 2.763 ± 0.532
3.743GlnLeu: 3.743 ± 0.702
1.337GlnMet: 1.337 ± 0.482
1.693GlnAsn: 1.693 ± 0.324
0.713GlnPro: 0.713 ± 0.265
2.139GlnGln: 2.139 ± 0.593
1.782GlnArg: 1.782 ± 0.514
3.387GlnSer: 3.387 ± 0.736
2.674GlnThr: 2.674 ± 0.372
1.961GlnVal: 1.961 ± 0.498
0.98GlnTrp: 0.98 ± 0.372
1.871GlnTyr: 1.871 ± 0.414
0.0GlnXaa: 0.0 ± 0.0
Arg
2.228ArgAla: 2.228 ± 0.466
0.089ArgCys: 0.089 ± 0.1
2.317ArgAsp: 2.317 ± 0.457
2.406ArgGlu: 2.406 ± 0.447
1.693ArgPhe: 1.693 ± 0.382
2.941ArgGly: 2.941 ± 0.659
0.98ArgHis: 0.98 ± 0.378
3.297ArgIle: 3.297 ± 0.788
3.03ArgLys: 3.03 ± 0.753
3.565ArgLeu: 3.565 ± 0.728
0.98ArgMet: 0.98 ± 0.31
2.139ArgAsn: 2.139 ± 0.396
1.337ArgPro: 1.337 ± 0.325
1.961ArgGln: 1.961 ± 0.438
1.871ArgArg: 1.871 ± 0.367
1.871ArgSer: 1.871 ± 0.391
1.961ArgThr: 1.961 ± 0.329
2.05ArgVal: 2.05 ± 0.497
0.624ArgTrp: 0.624 ± 0.193
1.871ArgTyr: 1.871 ± 0.422
0.0ArgXaa: 0.0 ± 0.0
Ser
5.704SerAla: 5.704 ± 1.124
0.356SerCys: 0.356 ± 0.168
4.099SerAsp: 4.099 ± 0.777
4.812SerGlu: 4.812 ± 0.605
2.852SerPhe: 2.852 ± 0.421
7.397SerGly: 7.397 ± 1.698
1.159SerHis: 1.159 ± 0.321
3.654SerIle: 3.654 ± 0.728
5.793SerLys: 5.793 ± 0.851
4.189SerLeu: 4.189 ± 0.569
2.05SerMet: 2.05 ± 0.41
6.238SerAsn: 6.238 ± 1.107
1.248SerPro: 1.248 ± 0.317
3.119SerGln: 3.119 ± 0.734
2.139SerArg: 2.139 ± 0.436
5.614SerSer: 5.614 ± 1.507
5.08SerThr: 5.08 ± 0.729
4.367SerVal: 4.367 ± 0.629
1.248SerTrp: 1.248 ± 0.409
1.871SerTyr: 1.871 ± 0.494
0.0SerXaa: 0.0 ± 0.0
Thr
5.971ThrAla: 5.971 ± 0.981
0.267ThrCys: 0.267 ± 0.146
4.01ThrAsp: 4.01 ± 0.594
4.099ThrGlu: 4.099 ± 0.826
2.763ThrPhe: 2.763 ± 0.675
6.238ThrGly: 6.238 ± 1.161
0.802ThrHis: 0.802 ± 0.337
3.476ThrIle: 3.476 ± 0.448
4.812ThrLys: 4.812 ± 0.823
4.545ThrLeu: 4.545 ± 0.485
1.069ThrMet: 1.069 ± 0.336
4.723ThrAsn: 4.723 ± 0.935
2.228ThrPro: 2.228 ± 0.58
2.317ThrGln: 2.317 ± 0.51
1.426ThrArg: 1.426 ± 0.31
6.06ThrSer: 6.06 ± 0.999
5.882ThrThr: 5.882 ± 1.261
5.614ThrVal: 5.614 ± 1.28
0.356ThrTrp: 0.356 ± 0.171
2.139ThrTyr: 2.139 ± 0.463
0.0ThrXaa: 0.0 ± 0.0
Val
5.525ValAla: 5.525 ± 1.386
0.98ValCys: 0.98 ± 0.262
2.852ValAsp: 2.852 ± 0.407
3.297ValGlu: 3.297 ± 0.699
1.871ValPhe: 1.871 ± 0.316
3.921ValGly: 3.921 ± 0.593
0.802ValHis: 0.802 ± 0.312
2.852ValIle: 2.852 ± 0.485
4.278ValLys: 4.278 ± 0.506
4.189ValLeu: 4.189 ± 0.752
1.426ValMet: 1.426 ± 0.306
5.347ValAsn: 5.347 ± 0.954
2.406ValPro: 2.406 ± 0.499
2.852ValGln: 2.852 ± 0.589
2.406ValArg: 2.406 ± 0.411
4.545ValSer: 4.545 ± 0.67
4.367ValThr: 4.367 ± 0.694
3.387ValVal: 3.387 ± 0.641
0.624ValTrp: 0.624 ± 0.239
1.693ValTyr: 1.693 ± 0.408
0.0ValXaa: 0.0 ± 0.0
Trp
1.069TrpAla: 1.069 ± 0.286
0.089TrpCys: 0.089 ± 0.087
0.802TrpAsp: 0.802 ± 0.27
0.713TrpGlu: 0.713 ± 0.34
0.802TrpPhe: 0.802 ± 0.274
0.802TrpGly: 0.802 ± 0.224
0.089TrpHis: 0.089 ± 0.103
0.535TrpIle: 0.535 ± 0.242
1.069TrpLys: 1.069 ± 0.332
1.426TrpLeu: 1.426 ± 0.33
0.089TrpMet: 0.089 ± 0.083
0.356TrpAsn: 0.356 ± 0.226
0.0TrpPro: 0.0 ± 0.0
0.535TrpGln: 0.535 ± 0.175
0.446TrpArg: 0.446 ± 0.231
0.891TrpSer: 0.891 ± 0.306
0.535TrpThr: 0.535 ± 0.233
0.98TrpVal: 0.98 ± 0.276
0.089TrpTrp: 0.089 ± 0.118
0.178TrpTyr: 0.178 ± 0.121
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.941TyrAla: 2.941 ± 0.427
0.446TyrCys: 0.446 ± 0.242
2.317TyrAsp: 2.317 ± 0.429
1.604TyrGlu: 1.604 ± 0.36
1.069TyrPhe: 1.069 ± 0.319
2.05TyrGly: 2.05 ± 0.386
0.535TyrHis: 0.535 ± 0.23
2.139TyrIle: 2.139 ± 0.48
2.852TyrLys: 2.852 ± 0.537
2.406TyrLeu: 2.406 ± 0.425
0.713TyrMet: 0.713 ± 0.279
1.871TyrAsn: 1.871 ± 0.414
1.069TyrPro: 1.069 ± 0.318
1.426TyrGln: 1.426 ± 0.361
1.159TyrArg: 1.159 ± 0.308
2.139TyrSer: 2.139 ± 0.446
2.941TyrThr: 2.941 ± 0.752
2.228TyrVal: 2.228 ± 0.468
0.802TyrTrp: 0.802 ± 0.219
1.515TyrTyr: 1.515 ± 0.37
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 45 proteins (11222 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski