Piper yellow mottle virus
Average proteome isoelectric point is 6.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|T2BAL2|T2BAL2_9VIRU Reverse transcriptase OS=Piper yellow mottle virus OX=262957 PE=4 SV=1
MM1 pKa = 7.55 ASSGTGNYY9 pKa = 9.85 HH10 pKa = 5.98 YY11 pKa = 10.6 GSSSRR16 pKa = 11.84 SGATEE21 pKa = 3.38 PNARR25 pKa = 11.84 FDD27 pKa = 3.74 QSPEE31 pKa = 3.78 AGLTPSQPMAKK42 pKa = 9.49 LDD44 pKa = 3.6 QQYY47 pKa = 10.91 QDD49 pKa = 2.94 ALEE52 pKa = 4.33 RR53 pKa = 11.84 FTRR56 pKa = 11.84 TDD58 pKa = 3.39 CTSTRR63 pKa = 11.84 EE64 pKa = 4.06 WWNHH68 pKa = 3.53 LCQLKK73 pKa = 10.14 EE74 pKa = 4.24 LEE76 pKa = 4.4 GKK78 pKa = 8.05 ATKK81 pKa = 9.76 EE82 pKa = 3.74 AEE84 pKa = 4.0 EE85 pKa = 4.07 AMEE88 pKa = 4.1 VLVNLHH94 pKa = 5.17 QLKK97 pKa = 10.06 HH98 pKa = 6.0 AEE100 pKa = 4.24 CARR103 pKa = 11.84 LSRR106 pKa = 11.84 KK107 pKa = 8.1 NQQLKK112 pKa = 10.98 DD113 pKa = 4.0 SVPDD117 pKa = 3.54 YY118 pKa = 11.25 YY119 pKa = 10.99 QDD121 pKa = 3.42 QLEE124 pKa = 5.17 AIMMDD129 pKa = 3.96 DD130 pKa = 3.16 VALRR134 pKa = 11.84 QIAINLVDD142 pKa = 4.03 VIKK145 pKa = 10.61 SVRR148 pKa = 11.84 AKK150 pKa = 10.47 EE151 pKa = 3.84 LL152 pKa = 3.34
Molecular weight: 17.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.241
IPC2_protein 5.308
IPC_protein 5.232
Toseland 5.283
ProMoST 5.41
Dawson 5.283
Bjellqvist 5.385
Wikipedia 5.169
Rodwell 5.219
Grimsley 5.245
Solomon 5.283
Lehninger 5.245
Nozaki 5.436
DTASelect 5.575
Thurlkill 5.334
EMBOSS 5.283
Sillero 5.512
Patrickios 4.126
IPC_peptide 5.296
IPC2_peptide 5.512
IPC2.peptide.svr19 5.519
Protein with the highest isoelectric point:
>tr|T2BB23|T2BB23_9VIRU ORF2 OS=Piper yellow mottle virus OX=262957 PE=4 SV=1
MM1 pKa = 7.75 SYY3 pKa = 10.81 INTISTSAYY12 pKa = 9.76 KK13 pKa = 10.3 EE14 pKa = 3.8 ALQATEE20 pKa = 3.86 QLEE23 pKa = 4.4 EE24 pKa = 4.02 PALGFVKK31 pKa = 10.35 PRR33 pKa = 11.84 DD34 pKa = 3.71 HH35 pKa = 6.56 NRR37 pKa = 11.84 EE38 pKa = 4.24 GIQKK42 pKa = 10.2 SVTAVTKK49 pKa = 10.64 QNNTLIQLVVDD60 pKa = 3.65 LHH62 pKa = 6.27 EE63 pKa = 5.48 KK64 pKa = 10.59 INTLEE69 pKa = 4.01 EE70 pKa = 4.61 KK71 pKa = 10.22 IEE73 pKa = 4.16 KK74 pKa = 9.5 LRR76 pKa = 11.84 KK77 pKa = 9.34 EE78 pKa = 4.49 PPQSTGISADD88 pKa = 3.35 LVTKK92 pKa = 10.8 LEE94 pKa = 3.99 NLTLQDD100 pKa = 3.12 QGKK103 pKa = 9.37 KK104 pKa = 9.56 PKK106 pKa = 9.74 EE107 pKa = 4.03 SRR109 pKa = 11.84 GKK111 pKa = 10.25 RR112 pKa = 11.84 LVFKK116 pKa = 10.84 DD117 pKa = 3.2 PLEE120 pKa = 4.43 IIKK123 pKa = 10.56 AEE125 pKa = 3.95 KK126 pKa = 10.18 RR127 pKa = 11.84 KK128 pKa = 10.07 LDD130 pKa = 3.65 GPGPSKK136 pKa = 11.1 NSGGGPNHH144 pKa = 7.06 SIQAKK149 pKa = 8.17 PEE151 pKa = 3.76 KK152 pKa = 10.44 DD153 pKa = 3.07 AA154 pKa = 5.47
Molecular weight: 17.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.501
IPC2_protein 8.478
IPC_protein 8.419
Toseland 9.545
ProMoST 9.004
Dawson 9.648
Bjellqvist 9.194
Wikipedia 9.736
Rodwell 10.35
Grimsley 9.677
Solomon 9.721
Lehninger 9.706
Nozaki 9.472
DTASelect 9.209
Thurlkill 9.531
EMBOSS 9.911
Sillero 9.56
Patrickios 10.101
IPC_peptide 9.721
IPC2_peptide 7.556
IPC2.peptide.svr19 7.542
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
2366
135
1925
591.5
67.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.959 ± 0.826
1.817 ± 0.348
5.368 ± 0.691
8.538 ± 1.145
2.874 ± 0.603
5.579 ± 1.289
2.282 ± 0.189
6.974 ± 0.976
7.946 ± 1.364
8.58 ± 1.89
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.409 ± 0.403
4.691 ± 0.277
4.522 ± 0.543
5.41 ± 0.536
5.114 ± 0.257
6.298 ± 0.644
5.621 ± 0.143
5.875 ± 0.422
1.268 ± 0.292
2.874 ± 0.608
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here