Venturia effusa
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10819 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A517LB62|A0A517LB62_9PEZI Uncharacterized protein OS=Venturia effusa OX=50376 GN=FKW77_007434 PE=3 SV=1
MM1 pKa = 7.95 DD2 pKa = 5.76 NIEE5 pKa = 4.47 NTTSMGDD12 pKa = 3.98 LIASLGFDD20 pKa = 3.5 PFLVLSQDD28 pKa = 3.65 LTFSEE33 pKa = 5.05 YY34 pKa = 11.2 VDD36 pKa = 4.59 FDD38 pKa = 4.09 PSFTTSATAQHH49 pKa = 6.91 DD50 pKa = 4.14 SAMLAEE56 pKa = 5.67 AGDD59 pKa = 3.71 QSDD62 pKa = 2.5 WSTFYY67 pKa = 11.04 NSQLFAPSTAIALAPVGTEE86 pKa = 4.1 YY87 pKa = 10.94 DD88 pKa = 4.17 DD89 pKa = 6.35 KK90 pKa = 11.67 SDD92 pKa = 3.87 PDD94 pKa = 3.49 NRR96 pKa = 11.84 AQALIHH102 pKa = 6.14 PPKK105 pKa = 10.47 VNQQRR110 pKa = 11.84 QPYY113 pKa = 7.96 TFYY116 pKa = 11.44 NRR118 pKa = 11.84 LHH120 pKa = 6.12 NVADD124 pKa = 4.82 EE125 pKa = 4.33 YY126 pKa = 11.89
Molecular weight: 14.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.884
IPC_protein 3.859
Toseland 3.643
ProMoST 4.05
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.846
Rodwell 3.694
Grimsley 3.554
Solomon 3.859
Lehninger 3.821
Nozaki 3.999
DTASelect 4.266
Thurlkill 3.719
EMBOSS 3.846
Sillero 3.999
Patrickios 1.926
IPC_peptide 3.859
IPC2_peptide 3.961
IPC2.peptide.svr19 3.876
Protein with the highest isoelectric point:
>tr|A0A517LPH6|A0A517LPH6_9PEZI Uncharacterized protein OS=Venturia effusa OX=50376 GN=FKW77_000057 PE=4 SV=1
MM1 pKa = 7.58 ARR3 pKa = 11.84 TTTTRR8 pKa = 11.84 RR9 pKa = 11.84 PGLLARR15 pKa = 11.84 LRR17 pKa = 11.84 GAPKK21 pKa = 9.84 TVTTTHH27 pKa = 5.64 TTTTTTRR34 pKa = 11.84 RR35 pKa = 11.84 TPRR38 pKa = 11.84 SKK40 pKa = 10.3 RR41 pKa = 11.84 HH42 pKa = 4.82 VNNFNTTTGAPVQHH56 pKa = 6.74 HH57 pKa = 5.76 RR58 pKa = 11.84 RR59 pKa = 11.84 KK60 pKa = 10.47 VSMGDD65 pKa = 3.34 KK66 pKa = 10.63 VSGAMMRR73 pKa = 11.84 LRR75 pKa = 11.84 GSLTRR80 pKa = 11.84 RR81 pKa = 11.84 PGLKK85 pKa = 10.1 AAGTRR90 pKa = 11.84 RR91 pKa = 11.84 MHH93 pKa = 5.65 GTDD96 pKa = 2.86 GRR98 pKa = 11.84 GAHH101 pKa = 6.21 HH102 pKa = 6.44 VAAYY106 pKa = 9.84
Molecular weight: 11.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.374
IPC2_protein 10.906
IPC_protein 12.384
Toseland 12.544
ProMoST 13.042
Dawson 12.544
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.179
Grimsley 12.588
Solomon 13.042
Lehninger 12.954
Nozaki 12.544
DTASelect 12.544
Thurlkill 12.544
EMBOSS 13.042
Sillero 12.544
Patrickios 11.901
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.113
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10819
0
10819
5302218
50
8756
490.1
54.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.608 ± 0.019
1.163 ± 0.009
5.721 ± 0.017
6.356 ± 0.023
3.658 ± 0.014
6.829 ± 0.026
2.377 ± 0.009
4.955 ± 0.015
5.328 ± 0.021
8.598 ± 0.022
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.246 ± 0.009
3.741 ± 0.012
6.066 ± 0.021
4.04 ± 0.018
6.003 ± 0.021
8.266 ± 0.028
6.037 ± 0.017
5.921 ± 0.014
1.416 ± 0.008
2.67 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here