Glycocaulis profundi

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Maricaulales; Maricaulaceae; Glycocaulis

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3419 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A550JSQ9|A0A550JSQ9_9PROT ATP synthase protein I OS=Glycocaulis profundi OX=2518983 GN=FKB34_16175 PE=3 SV=1
MM1 pKa = 8.06RR2 pKa = 11.84ILLAATAVALASAPAYY18 pKa = 9.64AQARR22 pKa = 11.84GDD24 pKa = 3.57YY25 pKa = 10.49TFINQTAHH33 pKa = 7.57DD34 pKa = 5.02LDD36 pKa = 4.07LTVNTFFGNHH46 pKa = 5.91FFPDD50 pKa = 3.63PLLAFSSDD58 pKa = 3.03TGFAEE63 pKa = 4.36PSVTNLTGTIRR74 pKa = 11.84YY75 pKa = 9.79VNDD78 pKa = 4.46DD79 pKa = 3.39GTGCSFQTQVFWNPNPPPGFWTFAINPTAVGDD111 pKa = 3.89DD112 pKa = 4.27PEE114 pKa = 5.84DD115 pKa = 3.83VICDD119 pKa = 3.68VQFTSQDD126 pKa = 3.26PGTGEE131 pKa = 4.18FEE133 pKa = 4.02VDD135 pKa = 3.26LTIGGFF141 pKa = 3.4

Molecular weight: 15.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A550KIW5|A0A550KIW5_9PROT Uncharacterized protein OS=Glycocaulis profundi OX=2518983 GN=FKB34_08400 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.09QPSKK9 pKa = 8.8IVRR12 pKa = 11.84KK13 pKa = 9.04RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.73GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.35AGRR28 pKa = 11.84QIVARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.98RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3419

0

3419

1075312

29

1784

314.5

33.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.004 ± 0.073

0.752 ± 0.014

6.212 ± 0.035

6.54 ± 0.037

3.665 ± 0.026

9.16 ± 0.042

1.897 ± 0.021

4.576 ± 0.027

2.51 ± 0.041

9.651 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.392 ± 0.021

2.22 ± 0.028

5.41 ± 0.033

2.775 ± 0.026

7.77 ± 0.041

5.138 ± 0.031

4.88 ± 0.023

6.938 ± 0.036

1.414 ± 0.019

2.063 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski