Lactobacillus coryniformis subsp. coryniformis CECT 5711
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2023 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|J3JBU1|J3JBU1_9LACO Conjugative transposon protein OS=Lactobacillus coryniformis subsp. coryniformis CECT 5711 OX=1185325 GN=A11Y_37051 PE=4 SV=1
MM1 pKa = 6.35 EE2 pKa = 4.44 TVRR5 pKa = 11.84 VFIVNLGYY13 pKa = 10.9 YY14 pKa = 9.57 NQGKK18 pKa = 7.41 PTGKK22 pKa = 9.33 WFTPPLYY29 pKa = 9.43 PDD31 pKa = 5.54 AIAEE35 pKa = 4.01 QLEE38 pKa = 4.38 LKK40 pKa = 10.48 NDD42 pKa = 3.5 SEE44 pKa = 4.9 EE45 pKa = 4.28 YY46 pKa = 10.44 AIHH49 pKa = 7.62 DD50 pKa = 3.83 YY51 pKa = 9.66 EE52 pKa = 5.82 APFEE56 pKa = 4.18 IDD58 pKa = 3.38 RR59 pKa = 11.84 FDD61 pKa = 5.72 SIDD64 pKa = 3.83 EE65 pKa = 4.16 INRR68 pKa = 11.84 KK69 pKa = 8.59 YY70 pKa = 10.79 AALEE74 pKa = 3.7 RR75 pKa = 11.84 LEE77 pKa = 5.22 EE78 pKa = 4.16 YY79 pKa = 11.01 DD80 pKa = 4.14 FGADD84 pKa = 3.17 VSALIGEE91 pKa = 4.7 WFRR94 pKa = 11.84 DD95 pKa = 3.48 IEE97 pKa = 4.19 EE98 pKa = 4.21 LAEE101 pKa = 4.05 NAEE104 pKa = 4.85 DD105 pKa = 3.61 IIIYY109 pKa = 10.31 KK110 pKa = 10.11 GVSDD114 pKa = 4.09 MAEE117 pKa = 3.94 LAQDD121 pKa = 3.61 AVEE124 pKa = 4.42 SGAVFGDD131 pKa = 4.38 LPDD134 pKa = 3.48 QVIAYY139 pKa = 10.24 LDD141 pKa = 4.06 FEE143 pKa = 4.5 ALGRR147 pKa = 11.84 DD148 pKa = 3.59 MEE150 pKa = 4.46 IEE152 pKa = 4.03 GNYY155 pKa = 10.35 LVTNSAIYY163 pKa = 10.05 EE164 pKa = 4.22 YY165 pKa = 11.49 ANN167 pKa = 3.22
Molecular weight: 19.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.759
IPC2_protein 3.859
IPC_protein 3.821
Toseland 3.63
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.681
Rodwell 3.656
Grimsley 3.541
Solomon 3.783
Lehninger 3.732
Nozaki 3.91
DTASelect 4.075
Thurlkill 3.668
EMBOSS 3.694
Sillero 3.935
Patrickios 1.863
IPC_peptide 3.783
IPC2_peptide 3.923
IPC2.peptide.svr19 3.846
Protein with the highest isoelectric point:
>tr|J3JBT8|J3JBT8_9LACO Uncharacterized protein OS=Lactobacillus coryniformis subsp. coryniformis CECT 5711 OX=1185325 GN=A11Y_119389 PE=4 SV=1
MM1 pKa = 7.33 IQTEE5 pKa = 4.2 LATFGFGLINNRR17 pKa = 11.84 KK18 pKa = 8.99 PILFSRR24 pKa = 11.84 KK25 pKa = 8.78 YY26 pKa = 7.83 STSRR30 pKa = 11.84 YY31 pKa = 8.92 FLLISVAKK39 pKa = 9.91 MNDD42 pKa = 3.03 YY43 pKa = 11.53 DD44 pKa = 4.06 RR45 pKa = 11.84 FRR47 pKa = 11.84 GDD49 pKa = 4.19 FRR51 pKa = 11.84 EE52 pKa = 4.32 LSCKK56 pKa = 9.87 LRR58 pKa = 11.84 NCISSTQLASITARR72 pKa = 11.84 NIVFCKK78 pKa = 9.91 HH79 pKa = 4.82 RR80 pKa = 11.84 RR81 pKa = 11.84 ASISIII87 pKa = 3.55
Molecular weight: 10.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.346
IPC2_protein 9.692
IPC_protein 10.452
Toseland 10.599
ProMoST 10.277
Dawson 10.716
Bjellqvist 10.438
Wikipedia 10.921
Rodwell 10.965
Grimsley 10.774
Solomon 10.804
Lehninger 10.774
Nozaki 10.613
DTASelect 10.423
Thurlkill 10.599
EMBOSS 10.994
Sillero 10.643
Patrickios 10.716
IPC_peptide 10.818
IPC2_peptide 9.633
IPC2.peptide.svr19 8.493
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2023
0
2023
662235
66
1608
327.4
36.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.583 ± 0.062
0.504 ± 0.013
5.398 ± 0.052
5.037 ± 0.046
4.154 ± 0.042
6.723 ± 0.054
2.227 ± 0.023
6.701 ± 0.051
5.481 ± 0.048
10.385 ± 0.065
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.479 ± 0.024
4.18 ± 0.032
3.874 ± 0.033
5.496 ± 0.061
4.373 ± 0.041
5.3 ± 0.05
6.515 ± 0.044
7.146 ± 0.038
0.986 ± 0.022
3.459 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here