Streptomyces longwoodensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6937 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A101QNX5|A0A101QNX5_9ACTN SnoaL-like domain-containing protein OS=Streptomyces longwoodensis OX=68231 GN=AQJ30_33935 PE=4 SV=1
MM1 pKa = 7.6SSGASGGPAARR12 pKa = 11.84YY13 pKa = 9.05LYY15 pKa = 9.73QLNGAIPLSVANAGAGTGIDD35 pKa = 3.63VTPDD39 pKa = 2.78SGTNTLTVTAVAADD53 pKa = 3.93GAIGGSTNCHH63 pKa = 6.24FFAGPPPTAPDD74 pKa = 3.69GDD76 pKa = 4.05LTGDD80 pKa = 4.17GLPDD84 pKa = 3.5LTVVGAQAGLPSGLWLARR102 pKa = 11.84GTADD106 pKa = 3.52GQVDD110 pKa = 3.84PDD112 pKa = 3.67VTNVGGQGTGASSAGSASDD131 pKa = 3.17WDD133 pKa = 3.95GTQTITGHH141 pKa = 5.57FHH143 pKa = 6.16TGAGFNDD150 pKa = 3.5VLVYY154 pKa = 10.85APTRR158 pKa = 11.84GTGTILYY165 pKa = 7.45GTGDD169 pKa = 3.9GSALSPYY176 pKa = 9.98SGHH179 pKa = 5.82EE180 pKa = 4.2VNVSSLAFTDD190 pKa = 3.76EE191 pKa = 4.38SGVTGAPGSRR201 pKa = 11.84ATSIASGGEE210 pKa = 3.88LYY212 pKa = 9.31RR213 pKa = 11.84TVNDD217 pKa = 3.49MPRR220 pKa = 11.84AGLPDD225 pKa = 3.95LLMIVNGQLWDD236 pKa = 3.91EE237 pKa = 4.85PGLMAPGAFLGLDD250 pKa = 3.09NALPLTSTNPTGSGDD265 pKa = 3.11WTGWSLTSSLVDD277 pKa = 3.23GLPALFARR285 pKa = 11.84NAATGALYY293 pKa = 10.54YY294 pKa = 7.04YY295 pKa = 9.81TPQQLQDD302 pKa = 3.73LAYY305 pKa = 9.54GNAVTPLKK313 pKa = 10.61LADD316 pKa = 3.77SGYY319 pKa = 10.92DD320 pKa = 3.23AAAVPVLQAADD331 pKa = 4.26LNSDD335 pKa = 3.81GTPDD339 pKa = 3.79LRR341 pKa = 11.84TVSATGVTTAWILDD355 pKa = 3.89PAAGTLTPRR364 pKa = 11.84PAQSLNVPGGTAA376 pKa = 3.09

Molecular weight: 37.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A117QK81|A0A117QK81_9ACTN Uncharacterized protein OS=Streptomyces longwoodensis OX=68231 GN=AQJ30_36140 PE=4 SV=1
MM1 pKa = 7.47ANCRR5 pKa = 11.84RR6 pKa = 11.84TPVTAIAPKK15 pKa = 9.93AVAPRR20 pKa = 11.84VVAMARR26 pKa = 11.84TGAVGVAVPARR37 pKa = 11.84VAVVVTRR44 pKa = 11.84PRR46 pKa = 11.84TVVSAVGRR54 pKa = 11.84VAIRR58 pKa = 11.84ALARR62 pKa = 11.84AVAMTARR69 pKa = 11.84VGARR73 pKa = 11.84RR74 pKa = 11.84LMARR78 pKa = 11.84RR79 pKa = 11.84RR80 pKa = 11.84PGAVLVGRR88 pKa = 11.84AARR91 pKa = 3.8

Molecular weight: 9.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6937

0

6937

2309017

29

6656

332.9

35.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.955 ± 0.042

0.783 ± 0.008

6.175 ± 0.026

5.491 ± 0.03

2.629 ± 0.016

9.501 ± 0.032

2.414 ± 0.015

2.724 ± 0.022

1.932 ± 0.026

10.426 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.579 ± 0.012

1.625 ± 0.016

6.256 ± 0.031

2.773 ± 0.019

8.311 ± 0.034

4.848 ± 0.021

6.287 ± 0.028

8.692 ± 0.027

1.528 ± 0.014

2.071 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski