Amino acid dipepetide frequency for Streptomyces longwoodensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.22AlaAla: 22.22 ± 0.162	1.115AlaCys: 1.115 ± 0.022	8.812AlaAsp: 8.812 ± 0.079	8.573AlaGlu: 8.573 ± 0.107	3.46AlaPhe: 3.46 ± 0.043	13.478AlaGly: 13.478 ± 0.084	3.304AlaHis: 3.304 ± 0.039	2.93AlaIle: 2.93 ± 0.04	2.533AlaLys: 2.533 ± 0.044	15.316AlaLeu: 15.316 ± 0.128	2.27AlaMet: 2.27 ± 0.041	1.762AlaAsn: 1.762 ± 0.032	7.815AlaPro: 7.815 ± 0.081	3.967AlaGln: 3.967 ± 0.048	11.139AlaArg: 11.139 ± 0.085	6.009AlaSer: 6.009 ± 0.053	7.277AlaThr: 7.277 ± 0.064	12.777AlaVal: 12.777 ± 0.093	1.948AlaTrp: 1.948 ± 0.027	2.84AlaTyr: 2.84 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	1.132CysAla: 1.132 ± 0.023	0.094CysCys: 0.094 ± 0.008	0.458CysAsp: 0.458 ± 0.015	0.368CysGlu: 0.368 ± 0.012	0.206CysPhe: 0.206 ± 0.01	0.928CysGly: 0.928 ± 0.024	0.205CysHis: 0.205 ± 0.01	0.15CysIle: 0.15 ± 0.009	0.106CysLys: 0.106 ± 0.007	0.76CysLeu: 0.76 ± 0.019	0.109CysMet: 0.109 ± 0.006	0.139CysAsn: 0.139 ± 0.008	0.492CysPro: 0.492 ± 0.015	0.15CysGln: 0.15 ± 0.009	0.624CysArg: 0.624 ± 0.019	0.44CysSer: 0.44 ± 0.013	0.531CysThr: 0.531 ± 0.016	0.677CysVal: 0.677 ± 0.016	0.126CysTrp: 0.126 ± 0.008	0.141CysTyr: 0.141 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	8.276AspAla: 8.276 ± 0.068	0.424AspCys: 0.424 ± 0.014	3.878AspAsp: 3.878 ± 0.048	3.78AspGlu: 3.78 ± 0.048	1.603AspPhe: 1.603 ± 0.029	6.712AspGly: 6.712 ± 0.062	1.528AspHis: 1.528 ± 0.024	1.579AspIle: 1.579 ± 0.03	1.08AspLys: 1.08 ± 0.026	6.586AspLeu: 6.586 ± 0.067	0.746AspMet: 0.746 ± 0.019	0.924AspAsn: 0.924 ± 0.021	4.777AspPro: 4.777 ± 0.051	1.576AspGln: 1.576 ± 0.026	5.149AspArg: 5.149 ± 0.058	2.337AspSer: 2.337 ± 0.035	3.528AspThr: 3.528 ± 0.044	5.115AspVal: 5.115 ± 0.046	1.06AspTrp: 1.06 ± 0.021	1.094AspTyr: 1.094 ± 0.025	0.0AspXaa: 0.0 ± 0.0
Glu	7.341GluAla: 7.341 ± 0.088	0.355GluCys: 0.355 ± 0.01	2.99GluAsp: 2.99 ± 0.041	3.536GluGlu: 3.536 ± 0.059	1.362GluPhe: 1.362 ± 0.025	4.224GluGly: 4.224 ± 0.044	1.524GluHis: 1.524 ± 0.024	1.884GluIle: 1.884 ± 0.034	1.278GluLys: 1.278 ± 0.029	6.391GluLeu: 6.391 ± 0.068	0.808GluMet: 0.808 ± 0.019	0.904GluAsn: 0.904 ± 0.018	3.337GluPro: 3.337 ± 0.049	2.248GluGln: 2.248 ± 0.037	5.586GluArg: 5.586 ± 0.061	2.18GluSer: 2.18 ± 0.028	2.683GluThr: 2.683 ± 0.037	4.532GluVal: 4.532 ± 0.048	0.704GluTrp: 0.704 ± 0.017	1.043GluTyr: 1.043 ± 0.022	0.0GluXaa: 0.0 ± 0.0
Phe	3.622PheAla: 3.622 ± 0.043	0.248PheCys: 0.248 ± 0.011	1.911PheAsp: 1.911 ± 0.033	1.325PheGlu: 1.325 ± 0.025	0.832PhePhe: 0.832 ± 0.025	2.835PheGly: 2.835 ± 0.041	0.61PheHis: 0.61 ± 0.017	0.597PheIle: 0.597 ± 0.017	0.481PheLys: 0.481 ± 0.018	2.581PheLeu: 2.581 ± 0.033	0.356PheMet: 0.356 ± 0.013	0.504PheAsn: 0.504 ± 0.016	1.343PhePro: 1.343 ± 0.023	0.68PheGln: 0.68 ± 0.018	1.812PheArg: 1.812 ± 0.029	1.366PheSer: 1.366 ± 0.028	2.004PheThr: 2.004 ± 0.036	2.257PheVal: 2.257 ± 0.03	0.379PheTrp: 0.379 ± 0.013	0.544PheTyr: 0.544 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	11.684GlyAla: 11.684 ± 0.084	0.808GlyCys: 0.808 ± 0.022	5.412GlyAsp: 5.412 ± 0.05	4.875GlyGlu: 4.875 ± 0.053	2.797GlyPhe: 2.797 ± 0.038	8.895GlyGly: 8.895 ± 0.096	2.448GlyHis: 2.448 ± 0.039	3.058GlyIle: 3.058 ± 0.04	2.132GlyLys: 2.132 ± 0.038	9.532GlyLeu: 9.532 ± 0.076	1.829GlyMet: 1.829 ± 0.029	1.645GlyAsn: 1.645 ± 0.031	5.453GlyPro: 5.453 ± 0.069	2.588GlyGln: 2.588 ± 0.04	8.12GlyArg: 8.12 ± 0.061	5.212GlySer: 5.212 ± 0.059	6.863GlyThr: 6.863 ± 0.082	7.674GlyVal: 7.674 ± 0.066	1.741GlyTrp: 1.741 ± 0.029	2.247GlyTyr: 2.247 ± 0.033	0.0GlyXaa: 0.0 ± 0.0
His	2.942HisAla: 2.942 ± 0.041	0.214HisCys: 0.214 ± 0.01	1.46HisAsp: 1.46 ± 0.023	1.221HisGlu: 1.221 ± 0.026	0.652HisPhe: 0.652 ± 0.017	2.611HisGly: 2.611 ± 0.037	0.74HisHis: 0.74 ± 0.023	0.57HisIle: 0.57 ± 0.015	0.327HisLys: 0.327 ± 0.012	2.64HisLeu: 2.64 ± 0.04	0.304HisMet: 0.304 ± 0.011	0.343HisAsn: 0.343 ± 0.012	1.983HisPro: 1.983 ± 0.034	0.673HisGln: 0.673 ± 0.016	2.327HisArg: 2.327 ± 0.038	0.976HisSer: 0.976 ± 0.02	1.425HisThr: 1.425 ± 0.027	1.854HisVal: 1.854 ± 0.031	0.416HisTrp: 0.416 ± 0.014	0.466HisTyr: 0.466 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	4.207IleAla: 4.207 ± 0.053	0.228IleCys: 0.228 ± 0.009	2.027IleAsp: 2.027 ± 0.033	1.685IleGlu: 1.685 ± 0.03	0.549IlePhe: 0.549 ± 0.013	3.068IleGly: 3.068 ± 0.047	0.54IleHis: 0.54 ± 0.017	0.675IleIle: 0.675 ± 0.022	0.617IleLys: 0.617 ± 0.018	2.064IleLeu: 2.064 ± 0.037	0.373IleMet: 0.373 ± 0.015	0.622IleAsn: 0.622 ± 0.019	1.442IlePro: 1.442 ± 0.028	0.645IleGln: 0.645 ± 0.018	1.922IleArg: 1.922 ± 0.033	1.404IleSer: 1.404 ± 0.027	1.965IleThr: 1.965 ± 0.031	2.436IleVal: 2.436 ± 0.043	0.312IleTrp: 0.312 ± 0.014	0.461IleTyr: 0.461 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	2.654LysAla: 2.654 ± 0.049	0.113LysCys: 0.113 ± 0.008	1.271LysAsp: 1.271 ± 0.027	1.061LysGlu: 1.061 ± 0.026	0.388LysPhe: 0.388 ± 0.015	1.703LysGly: 1.703 ± 0.034	0.381LysHis: 0.381 ± 0.013	0.741LysIle: 0.741 ± 0.021	0.743LysLys: 0.743 ± 0.027	1.814LysLeu: 1.814 ± 0.03	0.329LysMet: 0.329 ± 0.012	0.466LysAsn: 0.466 ± 0.017	1.153LysPro: 1.153 ± 0.023	0.652LysGln: 0.652 ± 0.017	1.268LysArg: 1.268 ± 0.027	0.999LysSer: 0.999 ± 0.024	1.145LysThr: 1.145 ± 0.026	1.779LysVal: 1.779 ± 0.033	0.252LysTrp: 0.252 ± 0.011	0.406LysTyr: 0.406 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	15.496LeuAla: 15.496 ± 0.115	0.866LeuCys: 0.866 ± 0.018	6.955LeuAsp: 6.955 ± 0.061	4.785LeuGlu: 4.785 ± 0.052	2.53LeuPhe: 2.53 ± 0.038	9.237LeuGly: 9.237 ± 0.078	2.443LeuHis: 2.443 ± 0.034	2.8LeuIle: 2.8 ± 0.042	1.89LeuLys: 1.89 ± 0.037	11.52LeuLeu: 11.52 ± 0.118	1.544LeuMet: 1.544 ± 0.026	1.531LeuAsn: 1.531 ± 0.028	6.642LeuPro: 6.642 ± 0.059	2.236LeuGln: 2.236 ± 0.035	9.014LeuArg: 9.014 ± 0.09	5.155LeuSer: 5.155 ± 0.051	7.135LeuThr: 7.135 ± 0.058	9.215LeuVal: 9.215 ± 0.073	1.349LeuTrp: 1.349 ± 0.032	1.918LeuTyr: 1.918 ± 0.033	0.0LeuXaa: 0.0 ± 0.0
Met	2.085MetAla: 2.085 ± 0.03	0.13MetCys: 0.13 ± 0.007	0.835MetAsp: 0.835 ± 0.025	0.679MetGlu: 0.679 ± 0.017	0.416MetPhe: 0.416 ± 0.013	1.219MetGly: 1.219 ± 0.024	0.333MetHis: 0.333 ± 0.011	0.548MetIle: 0.548 ± 0.017	0.367MetLys: 0.367 ± 0.013	1.466MetLeu: 1.466 ± 0.027	0.27MetMet: 0.27 ± 0.01	0.371MetAsn: 0.371 ± 0.012	0.995MetPro: 0.995 ± 0.018	0.419MetGln: 0.419 ± 0.015	1.315MetArg: 1.315 ± 0.026	1.187MetSer: 1.187 ± 0.024	1.482MetThr: 1.482 ± 0.027	1.181MetVal: 1.181 ± 0.025	0.199MetTrp: 0.199 ± 0.009	0.288MetTyr: 0.288 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	2.085AsnAla: 2.085 ± 0.036	0.144AsnCys: 0.144 ± 0.008	0.907AsnAsp: 0.907 ± 0.022	0.72AsnGlu: 0.72 ± 0.019	0.437AsnPhe: 0.437 ± 0.015	1.806AsnGly: 1.806 ± 0.038	0.381AsnHis: 0.381 ± 0.013	0.554AsnIle: 0.554 ± 0.017	0.365AsnLys: 0.365 ± 0.015	1.512AsnLeu: 1.512 ± 0.034	0.246AsnMet: 0.246 ± 0.01	0.404AsnAsn: 0.404 ± 0.014	1.235AsnPro: 1.235 ± 0.023	0.477AsnGln: 0.477 ± 0.017	1.142AsnArg: 1.142 ± 0.025	0.825AsnSer: 0.825 ± 0.023	1.032AsnThr: 1.032 ± 0.023	1.332AsnVal: 1.332 ± 0.027	0.272AsnTrp: 0.272 ± 0.013	0.37AsnTyr: 0.37 ± 0.013	0.0AsnXaa: 0.0 ± 0.0
Pro	9.361ProAla: 9.361 ± 0.096	0.349ProCys: 0.349 ± 0.013	4.74ProAsp: 4.74 ± 0.05	4.263ProGlu: 4.263 ± 0.05	1.505ProPhe: 1.505 ± 0.022	7.012ProGly: 7.012 ± 0.064	1.58ProHis: 1.58 ± 0.03	1.048ProIle: 1.048 ± 0.024	1.024ProLys: 1.024 ± 0.024	5.505ProLeu: 5.505 ± 0.057	0.881ProMet: 0.881 ± 0.02	0.805ProAsn: 0.805 ± 0.02	3.776ProPro: 3.776 ± 0.065	1.819ProGln: 1.819 ± 0.037	4.284ProArg: 4.284 ± 0.052	3.222ProSer: 3.222 ± 0.044	3.306ProThr: 3.306 ± 0.046	5.707ProVal: 5.707 ± 0.052	0.912ProTrp: 0.912 ± 0.02	1.466ProTyr: 1.466 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	3.786GlnAla: 3.786 ± 0.05	0.175GlnCys: 0.175 ± 0.009	1.5GlnAsp: 1.5 ± 0.028	1.506GlnGlu: 1.506 ± 0.03	0.647GlnPhe: 0.647 ± 0.017	2.414GlnGly: 2.414 ± 0.036	0.677GlnHis: 0.677 ± 0.018	0.965GlnIle: 0.965 ± 0.023	0.604GlnLys: 0.604 ± 0.015	2.967GlnLeu: 2.967 ± 0.041	0.464GlnMet: 0.464 ± 0.017	0.456GlnAsn: 0.456 ± 0.016	1.685GlnPro: 1.685 ± 0.033	1.313GlnGln: 1.313 ± 0.035	2.462GlnArg: 2.462 ± 0.035	1.214GlnSer: 1.214 ± 0.024	1.352GlnThr: 1.352 ± 0.025	2.426GlnVal: 2.426 ± 0.037	0.511GlnTrp: 0.511 ± 0.017	0.603GlnTyr: 0.603 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	10.743ArgAla: 10.743 ± 0.1	0.6ArgCys: 0.6 ± 0.017	4.458ArgAsp: 4.458 ± 0.049	4.696ArgGlu: 4.696 ± 0.049	2.332ArgPhe: 2.332 ± 0.032	6.06ArgGly: 6.06 ± 0.05	2.326ArgHis: 2.326 ± 0.039	2.987ArgIle: 2.987 ± 0.037	1.468ArgLys: 1.468 ± 0.027	9.335ArgLeu: 9.335 ± 0.095	1.647ArgMet: 1.647 ± 0.025	1.244ArgAsn: 1.244 ± 0.025	5.42ArgPro: 5.42 ± 0.068	2.318ArgGln: 2.318 ± 0.03	8.253ArgArg: 8.253 ± 0.088	3.942ArgSer: 3.942 ± 0.045	5.818ArgThr: 5.818 ± 0.062	6.229ArgVal: 6.229 ± 0.062	1.409ArgTrp: 1.409 ± 0.025	1.828ArgTyr: 1.828 ± 0.029	0.0ArgXaa: 0.0 ± 0.0
Ser	6.73SerAla: 6.73 ± 0.055	0.406SerCys: 0.406 ± 0.015	2.628SerAsp: 2.628 ± 0.036	2.105SerGlu: 2.105 ± 0.031	1.443SerPhe: 1.443 ± 0.025	5.838SerGly: 5.838 ± 0.062	0.951SerHis: 0.951 ± 0.02	1.191SerIle: 1.191 ± 0.021	0.894SerLys: 0.894 ± 0.024	4.678SerLeu: 4.678 ± 0.053	0.958SerMet: 0.958 ± 0.023	0.809SerAsn: 0.809 ± 0.021	3.096SerPro: 3.096 ± 0.04	1.174SerGln: 1.174 ± 0.024	3.609SerArg: 3.609 ± 0.041	2.766SerSer: 2.766 ± 0.044	2.987SerThr: 2.987 ± 0.047	4.177SerVal: 4.177 ± 0.046	0.854SerTrp: 0.854 ± 0.02	1.184SerTyr: 1.184 ± 0.022	0.0SerXaa: 0.0 ± 0.0
Thr	9.509ThrAla: 9.509 ± 0.074	0.453ThrCys: 0.453 ± 0.014	3.838ThrAsp: 3.838 ± 0.048	3.105ThrGlu: 3.105 ± 0.035	1.634ThrPhe: 1.634 ± 0.029	6.978ThrGly: 6.978 ± 0.073	1.302ThrHis: 1.302 ± 0.022	1.483ThrIle: 1.483 ± 0.028	1.045ThrLys: 1.045 ± 0.025	5.885ThrLeu: 5.885 ± 0.058	0.846ThrMet: 0.846 ± 0.019	0.966ThrAsn: 0.966 ± 0.027	4.382ThrPro: 4.382 ± 0.053	1.371ThrGln: 1.371 ± 0.029	4.168ThrArg: 4.168 ± 0.042	3.129ThrSer: 3.129 ± 0.049	4.054ThrThr: 4.054 ± 0.063	6.369ThrVal: 6.369 ± 0.058	0.961ThrTrp: 0.961 ± 0.022	1.389ThrTyr: 1.389 ± 0.025	0.0ThrXaa: 0.0 ± 0.0
Val	11.043ValAla: 11.043 ± 0.088	0.78ValCys: 0.78 ± 0.02	5.251ValAsp: 5.251 ± 0.049	4.69ValGlu: 4.69 ± 0.052	2.41ValPhe: 2.41 ± 0.038	6.616ValGly: 6.616 ± 0.059	2.106ValHis: 2.106 ± 0.03	2.556ValIle: 2.556 ± 0.041	1.655ValLys: 1.655 ± 0.03	9.863ValLeu: 9.863 ± 0.087	1.314ValMet: 1.314 ± 0.027	1.567ValAsn: 1.567 ± 0.031	5.695ValPro: 5.695 ± 0.055	2.199ValGln: 2.199 ± 0.031	7.697ValArg: 7.697 ± 0.068	4.233ValSer: 4.233 ± 0.041	5.984ValThr: 5.984 ± 0.06	8.472ValVal: 8.472 ± 0.077	1.184ValTrp: 1.184 ± 0.024	1.606ValTyr: 1.606 ± 0.03	0.0ValXaa: 0.0 ± 0.0
Trp	1.726TrpAla: 1.726 ± 0.03	0.159TrpCys: 0.159 ± 0.008	0.846TrpAsp: 0.846 ± 0.023	0.752TrpGlu: 0.752 ± 0.022	0.483TrpPhe: 0.483 ± 0.015	1.062TrpGly: 1.062 ± 0.02	0.38TrpHis: 0.38 ± 0.014	0.52TrpIle: 0.52 ± 0.014	0.348TrpLys: 0.348 ± 0.014	1.779TrpLeu: 1.779 ± 0.032	0.263TrpMet: 0.263 ± 0.01	0.396TrpAsn: 0.396 ± 0.013	0.789TrpPro: 0.789 ± 0.022	0.63TrpGln: 0.63 ± 0.019	1.366TrpArg: 1.366 ± 0.026	0.957TrpSer: 0.957 ± 0.02	1.117TrpThr: 1.117 ± 0.024	0.991TrpVal: 0.991 ± 0.025	0.347TrpTrp: 0.347 ± 0.014	0.369TrpTyr: 0.369 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.904TyrAla: 2.904 ± 0.038	0.175TyrCys: 0.175 ± 0.008	1.575TyrAsp: 1.575 ± 0.031	1.186TyrGlu: 1.186 ± 0.023	0.601TyrPhe: 0.601 ± 0.017	2.316TyrGly: 2.316 ± 0.034	0.386TyrHis: 0.386 ± 0.013	0.405TyrIle: 0.405 ± 0.015	0.362TyrLys: 0.362 ± 0.013	2.068TyrLeu: 2.068 ± 0.032	0.228TyrMet: 0.228 ± 0.01	0.388TyrAsn: 0.388 ± 0.016	1.051TyrPro: 1.051 ± 0.026	0.591TyrGln: 0.591 ± 0.018	1.858TyrArg: 1.858 ± 0.03	0.924TyrSer: 0.924 ± 0.021	1.179TyrThr: 1.179 ± 0.024	1.722TyrVal: 1.722 ± 0.029	0.343TyrTrp: 0.343 ± 0.012	0.448TyrTyr: 0.448 ± 0.018	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6937 proteins (2309017 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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