Bean necrotic mosaic virus
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I3PCT2|I3PCT2_9VIRU Non-structural movement protein OS=Bean necrotic mosaic virus OX=1033976 GN=NSm PE=4 SV=1
MM1 pKa = 7.61 GFRR4 pKa = 11.84 EE5 pKa = 4.3 VFVIICLFCLNLVFLDD21 pKa = 4.47 FSINAHH27 pKa = 4.93 TRR29 pKa = 11.84 GDD31 pKa = 3.76 HH32 pKa = 6.28 EE33 pKa = 4.68 LSQEE37 pKa = 3.74 ISFEE41 pKa = 4.25 DD42 pKa = 3.88 LSKK45 pKa = 11.29 DD46 pKa = 3.39 AVEE49 pKa = 4.34 SATLLGVDD57 pKa = 4.74 GKK59 pKa = 10.88 FKK61 pKa = 10.88 SGPLLDD67 pKa = 5.34 KK68 pKa = 10.9 PDD70 pKa = 4.83 IDD72 pKa = 5.03 DD73 pKa = 3.78 QMIPNRR79 pKa = 11.84 GKK81 pKa = 8.94 RR82 pKa = 11.84 TEE84 pKa = 4.39 PSSSSPPSSEE94 pKa = 3.6 QLTCKK99 pKa = 10.45 RR100 pKa = 11.84 INSNNCKK107 pKa = 9.82 IVGKK111 pKa = 8.87 TSFNFYY117 pKa = 10.53 YY118 pKa = 10.32 QISDD122 pKa = 3.55 NEE124 pKa = 4.55 TTVSCLSSEE133 pKa = 4.18 SQDD136 pKa = 3.53 YY137 pKa = 10.71 KK138 pKa = 11.16 SCNPDD143 pKa = 2.72 AMQEE147 pKa = 4.06 FLSADD152 pKa = 3.6 VLPITTMLNKK162 pKa = 9.98 RR163 pKa = 11.84 VLSVGSKK170 pKa = 10.26 YY171 pKa = 10.66 FILNSADD178 pKa = 3.32 PSSYY182 pKa = 9.98 PVQDD186 pKa = 3.54 HH187 pKa = 6.69 GSVQLGLGNIYY198 pKa = 10.21 NVRR201 pKa = 11.84 LTGGCNITKK210 pKa = 10.05 VSLANPFYY218 pKa = 10.01 ITLSSTAKK226 pKa = 10.34 KK227 pKa = 10.32 CLCCKK232 pKa = 10.41 KK233 pKa = 10.31 IGSEE237 pKa = 4.23 EE238 pKa = 4.05 VNLMKK243 pKa = 10.59 FSSEE247 pKa = 3.75 KK248 pKa = 10.19 TIEE251 pKa = 4.0 LSEE254 pKa = 4.74 DD255 pKa = 3.71 SLDD258 pKa = 3.76 GTHH261 pKa = 6.92 VLLCGDD267 pKa = 4.22 RR268 pKa = 11.84 VSEE271 pKa = 4.56 IPKK274 pKa = 9.62 IDD276 pKa = 3.3 INKK279 pKa = 9.51 RR280 pKa = 11.84 NCLVKK285 pKa = 10.93 YY286 pKa = 9.93 KK287 pKa = 10.54 GKK289 pKa = 10.46 RR290 pKa = 11.84 YY291 pKa = 8.06 QQSACVHH298 pKa = 5.94 FNLLRR303 pKa = 11.84 YY304 pKa = 9.71 LIVSIILFFPVSWFLNKK321 pKa = 9.54 TKK323 pKa = 10.81 EE324 pKa = 5.11 SMFLWYY330 pKa = 9.95 DD331 pKa = 2.88 ILGIVLYY338 pKa = 9.88 PLLYY342 pKa = 10.06 ALNWIWQYY350 pKa = 11.16 FPLKK354 pKa = 10.32 CSCCGNFSLITHH366 pKa = 6.83 KK367 pKa = 10.76 CYY369 pKa = 10.44 EE370 pKa = 4.13 KK371 pKa = 10.77 CVCNLSKK378 pKa = 10.75 AKK380 pKa = 10.17 KK381 pKa = 8.88 EE382 pKa = 3.96 HH383 pKa = 7.27 AEE385 pKa = 3.98 TCQILTGKK393 pKa = 8.55 IFKK396 pKa = 9.59 MKK398 pKa = 9.57 EE399 pKa = 3.49 VKK401 pKa = 10.27 AEE403 pKa = 3.8 KK404 pKa = 10.35 RR405 pKa = 11.84 EE406 pKa = 4.31 AITPDD411 pKa = 3.72 EE412 pKa = 4.13 IVSLNSEE419 pKa = 4.09 QEE421 pKa = 3.94 KK422 pKa = 10.51 PPVIKK427 pKa = 10.41 SSRR430 pKa = 11.84 FQTFQTIINTKK441 pKa = 9.71 ISSEE445 pKa = 4.11 TLLLITKK452 pKa = 9.77 ILLGFLIFSQIPKK465 pKa = 8.83 TMAMSNEE472 pKa = 3.56 QALCVNYY479 pKa = 10.1 CRR481 pKa = 11.84 AQIGCSKK488 pKa = 10.09 FLWKK492 pKa = 10.72 NEE494 pKa = 3.85 EE495 pKa = 4.09 TCISRR500 pKa = 11.84 KK501 pKa = 8.59 DD502 pKa = 3.65 LKK504 pKa = 10.7 CNCVIGKK511 pKa = 8.41 EE512 pKa = 4.25 LISEE516 pKa = 3.97 EE517 pKa = 4.33 AYY519 pKa = 10.12 INGTRR524 pKa = 11.84 YY525 pKa = 9.55 AVSMPNTCMDD535 pKa = 3.84 DD536 pKa = 3.58 TCDD539 pKa = 3.49 VAEE542 pKa = 4.76 GDD544 pKa = 3.87 VEE546 pKa = 4.13 NLIVCRR552 pKa = 11.84 LGCKK556 pKa = 9.8 KK557 pKa = 10.28 LRR559 pKa = 11.84 SLKK562 pKa = 8.65 TAKK565 pKa = 9.98 LSKK568 pKa = 9.48 RR569 pKa = 11.84 TFSTGIFSQSLSMNQIYY586 pKa = 8.05 STLRR590 pKa = 11.84 MQDD593 pKa = 3.19 GYY595 pKa = 10.71 IDD597 pKa = 3.81 SLEE600 pKa = 4.22 ALSTLGDD607 pKa = 3.77 PPSDD611 pKa = 3.07 KK612 pKa = 10.71 VVQIDD617 pKa = 3.59 DD618 pKa = 5.51 DD619 pKa = 4.02 IPEE622 pKa = 5.29 GILPRR627 pKa = 11.84 QSFVYY632 pKa = 10.48 SSVVDD637 pKa = 3.84 GKK639 pKa = 10.96 YY640 pKa = 9.88 RR641 pKa = 11.84 YY642 pKa = 10.12 LMSFDD647 pKa = 5.65 AIQSSGYY654 pKa = 8.56 VYY656 pKa = 10.95 SMKK659 pKa = 9.71 MLKK662 pKa = 8.59 WHH664 pKa = 7.13 PPQEE668 pKa = 3.85 LVVYY672 pKa = 9.66 VKK674 pKa = 10.52 EE675 pKa = 4.22 AGVTYY680 pKa = 9.21 TIKK683 pKa = 10.48 PLYY686 pKa = 8.21 HH687 pKa = 6.44 TAPISSTHH695 pKa = 3.84 THH697 pKa = 6.18 VYY699 pKa = 6.48 TTCTGNCEE707 pKa = 4.41 TCRR710 pKa = 11.84 KK711 pKa = 7.53 EE712 pKa = 4.37 HH713 pKa = 6.76 PLSGYY718 pKa = 9.12 QDD720 pKa = 3.78 YY721 pKa = 11.11 CISPTSNWGCEE732 pKa = 4.03 EE733 pKa = 4.63 LGCLAIGEE741 pKa = 4.66 GSTCGYY747 pKa = 9.08 CRR749 pKa = 11.84 NVYY752 pKa = 9.95 DD753 pKa = 4.19 LSKK756 pKa = 9.92 MYY758 pKa = 10.51 SINQVLSSHH767 pKa = 5.38 VTVKK771 pKa = 10.58 VCFKK775 pKa = 10.39 GFAGATCQTITDD787 pKa = 4.85 EE788 pKa = 4.58 IPYY791 pKa = 9.79 QNSYY795 pKa = 9.12 YY796 pKa = 10.34 QISLDD801 pKa = 3.9 ADD803 pKa = 3.8 LHH805 pKa = 6.72 NDD807 pKa = 3.56 DD808 pKa = 5.42 LGSGSRR814 pKa = 11.84 IAIDD818 pKa = 3.64 PNGLIMKK825 pKa = 9.71 GNIANLKK832 pKa = 10.19 DD833 pKa = 3.1 SSTAFGHH840 pKa = 5.9 PQLDD844 pKa = 3.63 KK845 pKa = 10.87 TGKK848 pKa = 10.32 LLFGKK853 pKa = 10.51 ANLDD857 pKa = 3.78 LNDD860 pKa = 4.07 FTWSCAVIGSKK871 pKa = 10.46 HH872 pKa = 5.9 VDD874 pKa = 3.02 IKK876 pKa = 11.06 KK877 pKa = 10.21 CGYY880 pKa = 8.35 DD881 pKa = 3.18 TYY883 pKa = 10.7 HH884 pKa = 6.8 QYY886 pKa = 10.67 IGLEE890 pKa = 4.46 PISNHH895 pKa = 5.13 YY896 pKa = 10.4 ASEE899 pKa = 4.08 ISDD902 pKa = 3.17 EE903 pKa = 4.13 KK904 pKa = 11.45 LFVKK908 pKa = 10.41 KK909 pKa = 10.34 DD910 pKa = 3.81 FKK912 pKa = 11.01 VGKK915 pKa = 9.92 INLIVDD921 pKa = 4.46 LPSEE925 pKa = 4.35 LFKK928 pKa = 11.3 QPAKK932 pKa = 10.09 KK933 pKa = 10.15 PKK935 pKa = 9.4 VSLINSEE942 pKa = 4.45 CKK944 pKa = 10.48 GCLSCGSGIKK954 pKa = 9.1 CTLEE958 pKa = 3.87 MTSDD962 pKa = 3.91 SIFAGNINWEE972 pKa = 4.33 HH973 pKa = 6.19 CTSEE977 pKa = 4.11 PEE979 pKa = 4.05 YY980 pKa = 10.69 IAMKK984 pKa = 10.11 KK985 pKa = 9.89 GSNIIKK991 pKa = 10.53 VNMFCLKK998 pKa = 10.5 NPSLTKK1004 pKa = 10.1 MILEE1008 pKa = 4.27 PSGDD1012 pKa = 3.52 SDD1014 pKa = 4.67 LSLEE1018 pKa = 4.14 FEE1020 pKa = 4.31 TRR1022 pKa = 11.84 NVEE1025 pKa = 4.14 IIDD1028 pKa = 3.82 PEE1030 pKa = 4.43 TIIDD1034 pKa = 3.98 HH1035 pKa = 6.9 NDD1037 pKa = 3.07 DD1038 pKa = 3.8 AFNEE1042 pKa = 4.22 EE1043 pKa = 3.5 VDD1045 pKa = 3.8 YY1046 pKa = 11.77 DD1047 pKa = 3.76 NDD1049 pKa = 3.75 TSFKK1053 pKa = 11.12 SLWDD1057 pKa = 3.63 YY1058 pKa = 11.37 LKK1060 pKa = 11.28 SPFNWIASFFGDD1072 pKa = 3.47 FFEE1075 pKa = 4.37 IVRR1078 pKa = 11.84 VLLVLACCVFAAICLSKK1095 pKa = 10.74 LFEE1098 pKa = 4.19 ICRR1101 pKa = 11.84 EE1102 pKa = 4.08 YY1103 pKa = 11.12 YY1104 pKa = 9.97 KK1105 pKa = 10.29 QEE1107 pKa = 3.98 HH1108 pKa = 5.84 YY1109 pKa = 10.39 KK1110 pKa = 10.79 KK1111 pKa = 10.41 SVKK1114 pKa = 10.14 SDD1116 pKa = 3.32 LKK1118 pKa = 11.59 DD1119 pKa = 3.23 MDD1121 pKa = 4.4 EE1122 pKa = 4.95 EE1123 pKa = 5.15 DD1124 pKa = 4.51 PEE1126 pKa = 4.79 DD1127 pKa = 4.63 PEE1129 pKa = 7.43 DD1130 pKa = 4.32 ILGNLKK1136 pKa = 9.95 NQITEE1141 pKa = 3.87 DD1142 pKa = 3.03 TLYY1145 pKa = 10.88 RR1146 pKa = 11.84 RR1147 pKa = 11.84 NLKK1150 pKa = 9.62 PKK1152 pKa = 9.93 KK1153 pKa = 10.3 SPFDD1157 pKa = 3.52 SVMTT1161 pKa = 4.39
Molecular weight: 130.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.458
IPC2_protein 5.614
IPC_protein 5.639
Toseland 5.792
ProMoST 5.944
Dawson 5.842
Bjellqvist 5.893
Wikipedia 5.817
Rodwell 5.804
Grimsley 5.817
Solomon 5.842
Lehninger 5.83
Nozaki 6.084
DTASelect 6.249
Thurlkill 6.211
EMBOSS 6.173
Sillero 6.173
Patrickios 3.592
IPC_peptide 5.855
IPC2_peptide 6.148
IPC2.peptide.svr19 6.184
Protein with the highest isoelectric point:
>tr|I3PCT0|I3PCT0_9VIRU Non-structural silencing protein OS=Bean necrotic mosaic virus OX=1033976 GN=NSs PE=4 SV=1
MM1 pKa = 7.62 GSNALKK7 pKa = 10.91 LNAEE11 pKa = 4.11 NLKK14 pKa = 10.72 KK15 pKa = 10.66 LLLFNDD21 pKa = 3.6 EE22 pKa = 4.85 VEE24 pKa = 4.98 FEE26 pKa = 4.17 QQSTGFKK33 pKa = 10.56 FVDD36 pKa = 4.12 FCKK39 pKa = 10.43 AHH41 pKa = 6.73 EE42 pKa = 4.21 KK43 pKa = 11.11 DD44 pKa = 3.38 KK45 pKa = 11.32 FNPTSALTFLKK56 pKa = 10.23 NRR58 pKa = 11.84 KK59 pKa = 9.03 AIYY62 pKa = 9.37 SLCKK66 pKa = 10.41 ASTFNYY72 pKa = 9.58 GVYY75 pKa = 9.98 EE76 pKa = 3.99 IKK78 pKa = 10.68 KK79 pKa = 9.76 GDD81 pKa = 3.58 KK82 pKa = 10.15 ATDD85 pKa = 3.02 TDD87 pKa = 4.46 FTFKK91 pKa = 10.94 RR92 pKa = 11.84 LDD94 pKa = 2.99 AFLRR98 pKa = 11.84 VKK100 pKa = 10.82 LLTHH104 pKa = 6.11 CTKK107 pKa = 10.11 IWDD110 pKa = 4.08 GTDD113 pKa = 3.41 PNAQTALCSEE123 pKa = 4.7 LAKK126 pKa = 10.52 IPIVKK131 pKa = 10.23 AYY133 pKa = 9.87 GLEE136 pKa = 4.08 VSDD139 pKa = 4.84 KK140 pKa = 8.6 QKK142 pKa = 11.11 CYY144 pKa = 10.27 IAIVLGGNLSLLASLPGCEE163 pKa = 4.22 VACFALAIFQDD174 pKa = 3.87 LKK176 pKa = 11.25 KK177 pKa = 10.61 NEE179 pKa = 4.5 LGIKK183 pKa = 10.03 SDD185 pKa = 5.3 FDD187 pKa = 3.91 TKK189 pKa = 11.04 DD190 pKa = 2.87 QAGRR194 pKa = 11.84 VAAVLDD200 pKa = 3.69 AKK202 pKa = 10.92 NFVFGEE208 pKa = 4.22 PEE210 pKa = 3.69 NEE212 pKa = 4.03 KK213 pKa = 10.54 LKK215 pKa = 10.92 KK216 pKa = 9.43 IAGILKK222 pKa = 10.03 EE223 pKa = 4.03 MTPTRR228 pKa = 11.84 RR229 pKa = 11.84 GHH231 pKa = 6.78 AALTKK236 pKa = 10.07 YY237 pKa = 10.63 AEE239 pKa = 4.1 QLSIISGVIGVTFEE253 pKa = 4.04 MPGTSKK259 pKa = 10.94 AKK261 pKa = 9.39 EE262 pKa = 4.31 SKK264 pKa = 10.18 NHH266 pKa = 5.44 GFMSS270 pKa = 4.01
Molecular weight: 29.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.863
IPC2_protein 8.039
IPC_protein 7.892
Toseland 8.068
ProMoST 8.478
Dawson 8.785
Bjellqvist 8.902
Wikipedia 8.77
Rodwell 8.931
Grimsley 7.892
Solomon 8.916
Lehninger 8.916
Nozaki 9.194
DTASelect 8.712
Thurlkill 8.858
EMBOSS 9.004
Sillero 9.092
Patrickios 4.851
IPC_peptide 8.916
IPC2_peptide 7.571
IPC2.peptide.svr19 7.756
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
5119
270
2932
1023.8
116.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.77 ± 0.726
2.559 ± 0.664
5.958 ± 0.247
6.896 ± 0.354
4.61 ± 0.415
4.884 ± 0.614
1.739 ± 0.15
7.111 ± 0.249
9.142 ± 0.449
9.807 ± 0.411
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.087 ± 0.554
5.997 ± 0.379
3.028 ± 0.38
2.774 ± 0.126
3.126 ± 0.431
9.631 ± 0.544
5.802 ± 0.417
5.45 ± 0.532
0.664 ± 0.165
3.966 ± 0.369
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here