Streptococcus satellite phage Javan401
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 19 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZLN4|A0A4D5ZLN4_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan401 OX=2558680 GN=JavanS401_0016 PE=4 SV=1
MM1 pKa = 7.2 KK2 pKa = 9.64 TLSDD6 pKa = 3.59 TPKK9 pKa = 9.76 TFTFTTTLPNFEE21 pKa = 4.31 QAQKK25 pKa = 10.83 AGSALIGYY33 pKa = 7.56 MVGTYY38 pKa = 7.06 EE39 pKa = 4.84 QSVIDD44 pKa = 3.34 ITYY47 pKa = 9.06 TGNGNLKK54 pKa = 10.56 AEE56 pKa = 4.29 YY57 pKa = 9.86 TSDD60 pKa = 3.39 EE61 pKa = 4.19 DD62 pKa = 3.69 LAEE65 pKa = 4.16 SFEE68 pKa = 5.17 RR69 pKa = 11.84 ITNSLDD75 pKa = 3.16 HH76 pKa = 6.92 TDD78 pKa = 3.84 DD79 pKa = 4.1 FKK81 pKa = 11.46 DD82 pKa = 3.86 YY83 pKa = 11.57 EE84 pKa = 4.37 EE85 pKa = 5.91 DD86 pKa = 3.77 EE87 pKa = 4.42 PTLKK91 pKa = 10.85 GDD93 pKa = 3.89 LDD95 pKa = 4.15 TYY97 pKa = 9.8 TALIGSFDD105 pKa = 3.91 TLGQAQAFTEE115 pKa = 4.03 NLDD118 pKa = 3.61 YY119 pKa = 11.3 EE120 pKa = 4.48 LTKK123 pKa = 11.04 GNNFIFEE130 pKa = 4.37 QLPDD134 pKa = 3.68 MVVLYY139 pKa = 8.61 VSPQDD144 pKa = 3.68 TIANSTIQKK153 pKa = 10.38 LEE155 pKa = 3.71 EE156 pKa = 4.37 AYY158 pKa = 10.58 VKK160 pKa = 10.78 HH161 pKa = 5.69 EE162 pKa = 4.52 ADD164 pKa = 3.66 YY165 pKa = 11.35 KK166 pKa = 10.89 PDD168 pKa = 3.63 TFRR171 pKa = 11.84 LNYY174 pKa = 9.8 HH175 pKa = 6.4 DD176 pKa = 3.95 MRR178 pKa = 11.84 VPQLQKK184 pKa = 10.54 LDD186 pKa = 4.04 KK187 pKa = 10.03 DD188 pKa = 3.98 TMISDD193 pKa = 4.27 IILYY197 pKa = 9.73 EE198 pKa = 4.7 LEE200 pKa = 4.7 LLDD203 pKa = 4.53 HH204 pKa = 7.54 ADD206 pKa = 3.89 RR207 pKa = 11.84 LLSDD211 pKa = 4.22 EE212 pKa = 5.14 PLPLDD217 pKa = 4.19 DD218 pKa = 3.33 QHH220 pKa = 8.96 GYY222 pKa = 7.78 EE223 pKa = 4.31 TVEE226 pKa = 3.89 LLGDD230 pKa = 3.78 EE231 pKa = 5.28 VIDD234 pKa = 4.6 LVKK237 pKa = 10.95 KK238 pKa = 10.86 LDD240 pKa = 3.52 TDD242 pKa = 3.62 KK243 pKa = 11.31 QFDD246 pKa = 4.55 GIHH249 pKa = 7.15 DD250 pKa = 4.28 YY251 pKa = 10.98 IIGGG255 pKa = 3.65
Molecular weight: 29.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.118
IPC2_protein 4.024
IPC_protein 4.024
Toseland 3.821
ProMoST 4.177
Dawson 4.012
Bjellqvist 4.164
Wikipedia 3.923
Rodwell 3.846
Grimsley 3.719
Solomon 3.999
Lehninger 3.961
Nozaki 4.113
DTASelect 4.355
Thurlkill 3.859
EMBOSS 3.935
Sillero 4.139
Patrickios 3.3
IPC_peptide 3.999
IPC2_peptide 4.126
IPC2.peptide.svr19 4.054
Protein with the highest isoelectric point:
>tr|A0A4D5ZS17|A0A4D5ZS17_9VIRU Integrase OS=Streptococcus satellite phage Javan401 OX=2558680 GN=JavanS401_0019 PE=3 SV=1
MM1 pKa = 7.92 KK2 pKa = 9.25 ITEE5 pKa = 4.22 YY6 pKa = 10.66 KK7 pKa = 10.04 KK8 pKa = 10.7 KK9 pKa = 10.7 NSTTVYY15 pKa = 9.95 RR16 pKa = 11.84 ANIYY20 pKa = 10.61 LGVDD24 pKa = 3.91 HH25 pKa = 7.01 ITGKK29 pKa = 10.18 KK30 pKa = 9.88 VKK32 pKa = 9.57 TSVTGRR38 pKa = 11.84 TKK40 pKa = 10.69 KK41 pKa = 9.61 EE42 pKa = 3.43 VKK44 pKa = 9.82 IRR46 pKa = 11.84 IKK48 pKa = 10.17 EE49 pKa = 3.91 AQHH52 pKa = 5.68 NFKK55 pKa = 10.67 TNGHH59 pKa = 5.51 TVTKK63 pKa = 10.16 AVPVEE68 pKa = 4.14 NYY70 pKa = 10.2 KK71 pKa = 10.55 EE72 pKa = 4.15 LAEE75 pKa = 4.41 LWLKK79 pKa = 10.56 SYY81 pKa = 11.07 EE82 pKa = 4.18 MTVKK86 pKa = 10.03 PQTFIATKK94 pKa = 10.58 RR95 pKa = 11.84 MIHH98 pKa = 5.41 NHH100 pKa = 7.26 LIPVFGDD107 pKa = 3.04 IKK109 pKa = 10.73 LDD111 pKa = 3.65 KK112 pKa = 10.92 LSVSYY117 pKa = 10.61 IQGHH121 pKa = 5.33 INEE124 pKa = 4.31 LSKK127 pKa = 11.19 EE128 pKa = 4.14 FVHH131 pKa = 7.02 FGTVHH136 pKa = 6.64 SINKK140 pKa = 9.37 RR141 pKa = 11.84 VLQYY145 pKa = 10.92 GVSLQLIPFNPARR158 pKa = 11.84 DD159 pKa = 3.83 VILPKK164 pKa = 10.34 AVKK167 pKa = 9.53 PDD169 pKa = 3.09 NKK171 pKa = 10.27 AIKK174 pKa = 10.24 FIDD177 pKa = 3.79 YY178 pKa = 10.68 DD179 pKa = 3.84 DD180 pKa = 5.34 LKK182 pKa = 11.66 ALMSYY187 pKa = 8.12 MEE189 pKa = 4.43 KK190 pKa = 10.66 LSNKK194 pKa = 9.25 KK195 pKa = 9.16 YY196 pKa = 10.55 SYY198 pKa = 10.49 YY199 pKa = 10.42 FDD201 pKa = 3.55 YY202 pKa = 11.2 VLYY205 pKa = 10.82 NILLATGCRR214 pKa = 11.84 FGEE217 pKa = 4.65 AIALTWSDD225 pKa = 3.04 IDD227 pKa = 4.54 FEE229 pKa = 4.55 NATIDD234 pKa = 3.43 INKK237 pKa = 9.88 NYY239 pKa = 9.76 NRR241 pKa = 11.84 LVDD244 pKa = 3.54 IVGTPKK250 pKa = 10.52 SKK252 pKa = 10.62 AGYY255 pKa = 9.05 RR256 pKa = 11.84 VISIDD261 pKa = 3.39 QKK263 pKa = 9.48 TVNLLRR269 pKa = 11.84 LYY271 pKa = 10.82 KK272 pKa = 10.28 NRR274 pKa = 11.84 QRR276 pKa = 11.84 QLFSEE281 pKa = 4.65 VGGEE285 pKa = 3.9 APKK288 pKa = 10.93 VVFATPTRR296 pKa = 11.84 KK297 pKa = 9.52 YY298 pKa = 9.69 QNTAIRR304 pKa = 11.84 QGSLDD309 pKa = 3.54 RR310 pKa = 11.84 RR311 pKa = 11.84 LKK313 pKa = 10.74 EE314 pKa = 3.58 IGCPRR319 pKa = 11.84 FTFHH323 pKa = 7.93 AFRR326 pKa = 11.84 HH327 pKa = 4.69 THH329 pKa = 6.92 ASLLLNAGISYY340 pKa = 10.68 KK341 pKa = 10.23 EE342 pKa = 3.61 LQYY345 pKa = 11.49 RR346 pKa = 11.84 LGHH349 pKa = 6.19 ATLAMTMDD357 pKa = 4.24 IYY359 pKa = 11.79 SHH361 pKa = 6.95 LSKK364 pKa = 10.92 DD365 pKa = 3.51 KK366 pKa = 10.41 EE367 pKa = 4.25 KK368 pKa = 10.93 EE369 pKa = 3.85 AVSYY373 pKa = 9.5 YY374 pKa = 10.62 EE375 pKa = 4.12 KK376 pKa = 10.7 AINSLL381 pKa = 3.57
Molecular weight: 43.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.118
IPC2_protein 9.224
IPC_protein 9.092
Toseland 9.809
ProMoST 9.531
Dawson 10.043
Bjellqvist 9.721
Wikipedia 10.218
Rodwell 10.482
Grimsley 10.131
Solomon 10.058
Lehninger 10.028
Nozaki 9.794
DTASelect 9.706
Thurlkill 9.882
EMBOSS 10.218
Sillero 9.955
Patrickios 7.585
IPC_peptide 10.058
IPC2_peptide 8.214
IPC2.peptide.svr19 8.294
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
19
0
19
2770
42
480
145.8
16.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.235 ± 0.689
0.686 ± 0.179
6.39 ± 0.695
7.473 ± 0.655
4.26 ± 0.42
4.765 ± 0.449
1.841 ± 0.244
8.195 ± 0.447
9.856 ± 0.79
9.603 ± 0.511
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.744 ± 0.371
6.065 ± 0.555
3.249 ± 0.44
3.682 ± 0.467
4.477 ± 0.362
4.693 ± 0.457
6.859 ± 0.526
4.079 ± 0.487
0.578 ± 0.237
5.271 ± 0.346
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here