uncultured phage_MedDCM-OCT-S31-C1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Nohivirus; Nohivirus S31C1

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6S4PGU2|A0A6S4PGU2_9CAUD DNA-directed RNA polymerase OS=uncultured phage_MedDCM-OCT-S31-C1 OX=2740800 PE=3 SV=1
MM1 pKa = 6.92TLSWLDD7 pKa = 5.0DD8 pKa = 3.68NDD10 pKa = 3.75GWWIQGWWHH19 pKa = 7.38GYY21 pKa = 7.01QQVGPDD27 pKa = 3.51LEE29 pKa = 4.49YY30 pKa = 11.0SVSEE34 pKa = 4.36DD35 pKa = 3.56YY36 pKa = 10.71MDD38 pKa = 4.1GFHH41 pKa = 6.94VGLRR45 pKa = 11.84IAKK48 pKa = 9.88LDD50 pKa = 3.5ACGVGDD56 pKa = 3.58QTPNDD61 pKa = 4.15FYY63 pKa = 11.0GQRR66 pKa = 11.84VGAA69 pKa = 4.53

Molecular weight: 7.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6S4PD20|A0A6S4PD20_9CAUD Exonuclease OS=uncultured phage_MedDCM-OCT-S31-C1 OX=2740800 PE=4 SV=1
MM1 pKa = 7.62IPLLSLVRR9 pKa = 11.84ANDD12 pKa = 3.82PQLSWLAVEE21 pKa = 5.06CLLHH25 pKa = 6.48CAEE28 pKa = 4.83HH29 pKa = 6.77PRR31 pKa = 11.84TIAEE35 pKa = 4.39LEE37 pKa = 4.1QLTGSANGNINRR49 pKa = 11.84AIRR52 pKa = 11.84SLTVWWDD59 pKa = 3.27ASKK62 pKa = 11.33KK63 pKa = 9.64EE64 pKa = 4.01VAKK67 pKa = 10.57PRR69 pKa = 11.84LHH71 pKa = 6.7LLQRR75 pKa = 11.84RR76 pKa = 11.84RR77 pKa = 11.84RR78 pKa = 11.84PKK80 pKa = 8.83PNRR83 pKa = 11.84GHH85 pKa = 7.16RR86 pKa = 11.84IHH88 pKa = 5.66VTKK91 pKa = 10.79AGFAFLHH98 pKa = 5.67EE99 pKa = 5.06AGLLFSAALL108 pKa = 3.57

Molecular weight: 12.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43

0

43

13635

44

1484

317.1

35.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.923 ± 0.436

1.1 ± 0.209

6.417 ± 0.306

6.469 ± 0.423

3.52 ± 0.268

8.148 ± 0.422

1.364 ± 0.188

4.378 ± 0.213

4.65 ± 0.35

8.009 ± 0.381

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.677 ± 0.216

4.437 ± 0.27

5.053 ± 0.18

5.442 ± 0.341

5.941 ± 0.341

5.691 ± 0.202

5.955 ± 0.557

6.102 ± 0.219

2.002 ± 0.181

2.721 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski