Thermosediminibacter oceani (strain ATCC BAA-1034 / DSM 16646 / JW/IW-1228P)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Thermosediminibacterales; Thermosediminibacteraceae; Thermosediminibacter; Thermosediminibacter oceani

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2151 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D9S078|D9S078_THEOJ Rubredoxin OS=Thermosediminibacter oceani (strain ATCC BAA-1034 / DSM 16646 / JW/IW-1228P) OX=555079 GN=Toce_0218 PE=3 SV=1
MM1 pKa = 7.55KK2 pKa = 10.43LYY4 pKa = 10.74VDD6 pKa = 3.58QDD8 pKa = 3.87LCISCGLCIDD18 pKa = 4.48ACPSVFSWNDD28 pKa = 2.89EE29 pKa = 4.21GKK31 pKa = 10.31SVAIDD36 pKa = 3.72GEE38 pKa = 4.69VPADD42 pKa = 3.62AEE44 pKa = 4.27NEE46 pKa = 3.81ARR48 pKa = 11.84EE49 pKa = 4.07ALEE52 pKa = 4.06NCPTEE57 pKa = 4.59AIKK60 pKa = 10.39EE61 pKa = 4.17AA62 pKa = 3.96

Molecular weight: 6.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D9S1F3|D9S1F3_THEOJ Pyruvate ferredoxin/flavodoxin oxidoreductase delta subunit OS=Thermosediminibacter oceani (strain ATCC BAA-1034 / DSM 16646 / JW/IW-1228P) OX=555079 GN=Toce_0453 PE=4 SV=1
MM1 pKa = 7.14KK2 pKa = 9.31QTYY5 pKa = 7.33QPRR8 pKa = 11.84KK9 pKa = 7.76RR10 pKa = 11.84YY11 pKa = 8.7RR12 pKa = 11.84KK13 pKa = 9.08KK14 pKa = 9.15VHH16 pKa = 5.85GFRR19 pKa = 11.84KK20 pKa = 9.98RR21 pKa = 11.84MSTKK25 pKa = 10.1SGRR28 pKa = 11.84NVIKK32 pKa = 10.48RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.22GRR39 pKa = 11.84KK40 pKa = 9.04RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2151

0

2151

637640

30

1823

296.4

33.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.763 ± 0.062

1.039 ± 0.023

5.081 ± 0.04

7.728 ± 0.061

4.186 ± 0.038

7.633 ± 0.05

1.531 ± 0.02

7.936 ± 0.049

7.176 ± 0.049

9.878 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.635 ± 0.025

3.795 ± 0.038

4.045 ± 0.03

2.49 ± 0.029

5.511 ± 0.048

5.188 ± 0.038

4.625 ± 0.031

7.707 ± 0.05

0.744 ± 0.017

3.309 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski