Sphingobium cloacae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingobium

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3852 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E1F2D1|A0A1E1F2D1_9SPHN Flagellar protein FlgJ OS=Sphingobium cloacae OX=120107 GN=SCLO_1016370 PE=4 SV=1
MM1 pKa = 7.42TYY3 pKa = 10.78VVTDD7 pKa = 2.96NCIRR11 pKa = 11.84CKK13 pKa = 11.03YY14 pKa = 7.82MDD16 pKa = 4.38CVEE19 pKa = 4.32VCPVDD24 pKa = 3.79CFYY27 pKa = 11.19EE28 pKa = 4.58GEE30 pKa = 4.18NMLVINPSEE39 pKa = 4.67CIDD42 pKa = 4.07CGVCEE47 pKa = 4.44PEE49 pKa = 4.82CPAEE53 pKa = 5.13AILPDD58 pKa = 3.96TEE60 pKa = 4.78SGLEE64 pKa = 3.79SWLEE68 pKa = 3.84LNAKK72 pKa = 9.95YY73 pKa = 10.31SAEE76 pKa = 3.93WPNITVKK83 pKa = 10.86GDD85 pKa = 3.33APADD89 pKa = 3.78ADD91 pKa = 3.59EE92 pKa = 4.44MSGVEE97 pKa = 4.01NKK99 pKa = 10.26LEE101 pKa = 4.26KK102 pKa = 10.57FFSPNPGAGDD112 pKa = 3.39

Molecular weight: 12.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E1EY52|A0A1E1EY52_9SPHN Acetate kinase OS=Sphingobium cloacae OX=120107 GN=ackA PE=3 SV=1
MM1 pKa = 6.92QVKK4 pKa = 9.42AHH6 pKa = 6.76KK7 pKa = 10.33LGLKK11 pKa = 7.83TAHH14 pKa = 7.05RR15 pKa = 11.84NPAPRR20 pKa = 11.84PRR22 pKa = 11.84LGGEE26 pKa = 4.13NLDD29 pKa = 3.38EE30 pKa = 5.17AIRR33 pKa = 11.84LRR35 pKa = 11.84EE36 pKa = 3.86VEE38 pKa = 4.0NWSFSAIGTHH48 pKa = 7.05FGICEE53 pKa = 3.73ASACNAVTIALCVRR67 pKa = 11.84RR68 pKa = 11.84GYY70 pKa = 10.62RR71 pKa = 11.84PAEE74 pKa = 4.07RR75 pKa = 11.84DD76 pKa = 3.0QHH78 pKa = 5.79GRR80 pKa = 11.84LTAEE84 pKa = 4.5GIEE87 pKa = 4.09RR88 pKa = 11.84LRR90 pKa = 11.84YY91 pKa = 9.26ALKK94 pKa = 10.45KK95 pKa = 9.73GYY97 pKa = 10.0KK98 pKa = 10.33GIDD101 pKa = 2.84IQLRR105 pKa = 11.84LGVSAACVSEE115 pKa = 3.49QRR117 pKa = 11.84RR118 pKa = 11.84RR119 pKa = 11.84YY120 pKa = 9.66NRR122 pKa = 11.84EE123 pKa = 3.08LLARR127 pKa = 11.84GKK129 pKa = 10.32AALPPPGGGEE139 pKa = 3.94AYY141 pKa = 10.52SGVKK145 pKa = 10.46LSPAKK150 pKa = 10.0RR151 pKa = 11.84RR152 pKa = 11.84QVEE155 pKa = 3.99EE156 pKa = 4.9LFLQGLGTQKK166 pKa = 9.78IAEE169 pKa = 4.16RR170 pKa = 11.84TGVSKK175 pKa = 9.39TSCTRR180 pKa = 11.84IRR182 pKa = 11.84GRR184 pKa = 11.84LIRR187 pKa = 11.84SLRR190 pKa = 11.84RR191 pKa = 11.84KK192 pKa = 10.22GEE194 pKa = 4.15SLPGCDD200 pKa = 2.98SCGVRR205 pKa = 11.84HH206 pKa = 5.43VHH208 pKa = 6.51AEE210 pKa = 3.79SARR213 pKa = 11.84FVTDD217 pKa = 3.21EE218 pKa = 4.18QKK220 pKa = 11.28DD221 pKa = 3.74LLRR224 pKa = 11.84AMLLDD229 pKa = 3.9RR230 pKa = 11.84VPVQRR235 pKa = 11.84AARR238 pKa = 11.84EE239 pKa = 3.99LAIGASTAYY248 pKa = 10.38RR249 pKa = 11.84LRR251 pKa = 11.84DD252 pKa = 3.39AFAAEE257 pKa = 3.96LAGEE261 pKa = 4.59GRR263 pKa = 11.84ALPPPRR269 pKa = 11.84RR270 pKa = 11.84PGRR273 pKa = 11.84VRR275 pKa = 11.84HH276 pKa = 5.42APMRR280 pKa = 11.84NSCWPPASPQEE291 pKa = 3.81IYY293 pKa = 10.99AFRR296 pKa = 11.84RR297 pKa = 11.84LLGCMGFAEE306 pKa = 5.8AKK308 pKa = 10.41AHH310 pKa = 5.14WQDD313 pKa = 2.95TRR315 pKa = 11.84RR316 pKa = 11.84EE317 pKa = 3.9EE318 pKa = 3.91ARR320 pKa = 11.84IARR323 pKa = 11.84EE324 pKa = 3.3AAATHH329 pKa = 6.64KK330 pKa = 10.21LTFEE334 pKa = 3.93EE335 pKa = 4.13QLAKK339 pKa = 10.42VASGEE344 pKa = 3.94LRR346 pKa = 11.84ITRR349 pKa = 11.84GFVRR353 pKa = 11.84NHH355 pKa = 6.55LEE357 pKa = 3.74PRR359 pKa = 11.84LPAQAVDD366 pKa = 3.37AA367 pKa = 4.95

Molecular weight: 40.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3852

0

3852

1264410

99

2202

328.2

35.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.116 ± 0.057

0.781 ± 0.013

5.994 ± 0.031

5.571 ± 0.038

3.468 ± 0.024

8.696 ± 0.039

2.074 ± 0.02

5.087 ± 0.023

3.085 ± 0.032

10.031 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.559 ± 0.021

2.432 ± 0.025

5.283 ± 0.027

3.227 ± 0.023

7.802 ± 0.046

5.282 ± 0.029

4.98 ± 0.03

6.923 ± 0.032

1.427 ± 0.017

2.183 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski