Sodalis endosymbiont of Henestaris halophilus
Average proteome isoelectric point is 7.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 712 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2C8CWW4|A0A2C8CWW4_9GAMM Uncharacterized protein OS=Sodalis endosymbiont of Henestaris halophilus OX=1929246 GN=HBA_0196 PE=4 SV=1
MM1 pKa = 7.64 FDD3 pKa = 3.14 VLIYY7 pKa = 10.85 LFEE10 pKa = 4.45 TYY12 pKa = 9.07 IHH14 pKa = 5.86 AVEE17 pKa = 4.26 MRR19 pKa = 11.84 VDD21 pKa = 3.62 QDD23 pKa = 4.01 KK24 pKa = 9.64 LTDD27 pKa = 3.71 DD28 pKa = 3.84 LTRR31 pKa = 11.84 VGFHH35 pKa = 6.65 QDD37 pKa = 3.36 DD38 pKa = 4.38 IYY40 pKa = 11.84 NALNWLEE47 pKa = 4.41 KK48 pKa = 10.75 LADD51 pKa = 3.96 LQDD54 pKa = 3.84 GQGRR58 pKa = 11.84 AVAFNADD65 pKa = 3.78 PLAMRR70 pKa = 11.84 IYY72 pKa = 10.58 SEE74 pKa = 4.58 EE75 pKa = 4.11 EE76 pKa = 3.6 NHH78 pKa = 7.33 CLDD81 pKa = 3.5 TCCRR85 pKa = 11.84 GFLLFLEE92 pKa = 4.79 QIQVLNLEE100 pKa = 4.22 TRR102 pKa = 11.84 EE103 pKa = 3.96 MVIDD107 pKa = 3.81 RR108 pKa = 11.84 VMALDD113 pKa = 3.6 AVEE116 pKa = 5.61 FDD118 pKa = 5.21 LEE120 pKa = 4.13 DD121 pKa = 4.08 LKK123 pKa = 11.14 WVVLMVLFNIPGCEE137 pKa = 3.64 NAYY140 pKa = 9.97 QQMEE144 pKa = 4.16 DD145 pKa = 3.7 LVFEE149 pKa = 4.16 EE150 pKa = 4.86 HH151 pKa = 6.71 VKK153 pKa = 10.51 HH154 pKa = 6.39 LHH156 pKa = 5.54
Molecular weight: 18.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.206
IPC2_protein 4.266
IPC_protein 4.215
Toseland 4.037
ProMoST 4.329
Dawson 4.177
Bjellqvist 4.329
Wikipedia 4.075
Rodwell 4.05
Grimsley 3.948
Solomon 4.177
Lehninger 4.139
Nozaki 4.291
DTASelect 4.482
Thurlkill 4.062
EMBOSS 4.101
Sillero 4.329
Patrickios 3.21
IPC_peptide 4.177
IPC2_peptide 4.317
IPC2.peptide.svr19 4.234
Protein with the highest isoelectric point:
>tr|A0A2C8CXS0|A0A2C8CXS0_9GAMM 50S ribosomal protein L22 OS=Sodalis endosymbiont of Henestaris halophilus OX=1929246 GN=rplV PE=3 SV=1
MM1 pKa = 7.23 NVIINLAPSEE11 pKa = 4.39 WFQEE15 pKa = 4.06 MRR17 pKa = 11.84 VGRR20 pKa = 11.84 YY21 pKa = 8.15 SKK23 pKa = 10.95 CDD25 pKa = 3.39 IILGMTVKK33 pKa = 10.57 LVLVARR39 pKa = 11.84 ALINLTRR46 pKa = 11.84 QLCISSAINAAHH58 pKa = 8.37 RR59 pKa = 11.84 IRR61 pKa = 11.84 TSMSATSLL69 pKa = 3.56
Molecular weight: 7.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.339
IPC2_protein 9.428
IPC_protein 10.101
Toseland 10.438
ProMoST 10.116
Dawson 10.555
Bjellqvist 10.292
Wikipedia 10.745
Rodwell 10.745
Grimsley 10.599
Solomon 10.687
Lehninger 10.657
Nozaki 10.496
DTASelect 10.262
Thurlkill 10.452
EMBOSS 10.833
Sillero 10.511
Patrickios 10.657
IPC_peptide 10.687
IPC2_peptide 9.663
IPC2.peptide.svr19 8.371
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
712
0
712
200869
30
1406
282.1
31.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.061 ± 0.09
1.362 ± 0.03
4.991 ± 0.063
5.234 ± 0.082
3.782 ± 0.062
6.703 ± 0.082
2.502 ± 0.041
7.478 ± 0.08
5.209 ± 0.076
10.85 ± 0.118
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.872 ± 0.042
4.536 ± 0.059
3.805 ± 0.047
4.154 ± 0.062
5.919 ± 0.071
5.959 ± 0.06
5.281 ± 0.052
7.062 ± 0.077
1.126 ± 0.034
3.114 ± 0.055
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here