Actinomyces bowdenii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Actinomyces

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2433 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3P1V7G8|A0A3P1V7G8_9ACTO Cell division protein FtsW OS=Actinomyces bowdenii OX=131109 GN=EII10_06110 PE=4 SV=1
MM1 pKa = 7.46LTGCQNTASTNDD13 pKa = 3.51SAPTPSEE20 pKa = 3.96SSTSLTMEE28 pKa = 4.71RR29 pKa = 11.84EE30 pKa = 4.1PGGLTEE36 pKa = 6.13SGDD39 pKa = 3.56QTDD42 pKa = 4.21PSDD45 pKa = 4.02PAAQTDD51 pKa = 3.75PATDD55 pKa = 3.61SDD57 pKa = 4.42SATPSAEE64 pKa = 4.04PTDD67 pKa = 4.01SCSGMSGEE75 pKa = 5.17EE76 pKa = 4.27ALQTWGGQVAPAKK89 pKa = 10.7DD90 pKa = 5.36DD91 pKa = 3.83DD92 pKa = 6.05PKK94 pKa = 10.53WQWNLEE100 pKa = 4.07AADD103 pKa = 3.47TSTYY107 pKa = 10.69DD108 pKa = 3.03EE109 pKa = 4.73CAALSWVVLGIHH121 pKa = 6.81GPTGSSPYY129 pKa = 10.51QIMLFHH135 pKa = 7.17HH136 pKa = 6.5GQYY139 pKa = 10.23IGVTSDD145 pKa = 2.98QAIAFAPQVARR156 pKa = 11.84IDD158 pKa = 3.98DD159 pKa = 3.93GSIEE163 pKa = 4.17VTYY166 pKa = 8.93TWTRR170 pKa = 11.84EE171 pKa = 4.1GEE173 pKa = 4.48TTASASGRR181 pKa = 11.84SVSVFTWDD189 pKa = 3.28EE190 pKa = 3.75ASGGVVHH197 pKa = 6.86SGEE200 pKa = 4.45WPPSVQQ206 pKa = 3.93

Molecular weight: 21.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3P1VB94|A0A3P1VB94_9ACTO Uncharacterized protein OS=Actinomyces bowdenii OX=131109 GN=EII10_01995 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2433

0

2433

860907

29

3193

353.8

37.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.075 ± 0.08

0.773 ± 0.013

5.428 ± 0.04

5.921 ± 0.047

2.34 ± 0.03

9.646 ± 0.047

2.1 ± 0.022

3.99 ± 0.034

1.575 ± 0.033

10.364 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.013 ± 0.021

1.562 ± 0.024

6.123 ± 0.048

3.22 ± 0.027

7.879 ± 0.06

5.95 ± 0.037

5.625 ± 0.041

8.068 ± 0.042

1.486 ± 0.022

1.862 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski