Trebonia kvetii
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8100 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6P2C751|A0A6P2C751_9ACTN Flavin reductase OS=Trebonia kvetii OX=2480626 GN=EAS64_06870 PE=4 SV=1
MM1 pKa = 7.01 PTATAGTTGATAAGVRR17 pKa = 11.84 GTPSPPPGFTTVFADD32 pKa = 4.92 DD33 pKa = 4.01 FNGPAGSAPSQQNWFYY49 pKa = 11.69 DD50 pKa = 3.21 LGTTFGSDD58 pKa = 3.39 QVNQTNSTSNTYY70 pKa = 10.97 LDD72 pKa = 3.8 GKK74 pKa = 10.65 GDD76 pKa = 4.39 LVMQANDD83 pKa = 3.54 TGGKK87 pKa = 5.09 WTAAEE92 pKa = 4.15 IEE94 pKa = 4.26 TTRR97 pKa = 11.84 DD98 pKa = 3.12 DD99 pKa = 3.91 FAAPPGGKK107 pKa = 9.72 LEE109 pKa = 3.96 ITASIEE115 pKa = 4.1 QPNPANGTGYY125 pKa = 9.86 WPAFWAGGTPIRR137 pKa = 11.84 NGGPWPQSGEE147 pKa = 3.56 IDD149 pKa = 3.39 MMEE152 pKa = 5.58 DD153 pKa = 3.03 INGLNEE159 pKa = 3.91 AAQTFHH165 pKa = 7.03 FGSSSSTQLGGEE177 pKa = 4.47 LMSCPDD183 pKa = 3.97 KK184 pKa = 11.4 ASTCQTGFHH193 pKa = 6.61 TYY195 pKa = 10.38 SVIIDD200 pKa = 3.61 RR201 pKa = 11.84 TNTSDD206 pKa = 3.9 EE207 pKa = 4.19 SLQFLMDD214 pKa = 3.34 GRR216 pKa = 11.84 IEE218 pKa = 4.01 ATYY221 pKa = 10.54 TEE223 pKa = 4.45 AQVGTSTWQAAIDD236 pKa = 3.61 HH237 pKa = 7.12 DD238 pKa = 4.81 FFILLDD244 pKa = 3.81 VVLGGTWPDD253 pKa = 3.56 NQCGCTSPTSATTPGGAMRR272 pKa = 11.84 VGCVAVYY279 pKa = 10.43 EE280 pKa = 4.39 EE281 pKa = 4.51 AANN284 pKa = 3.54
Molecular weight: 29.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.758
IPC2_protein 3.923
IPC_protein 3.91
Toseland 3.694
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.821
Rodwell 3.732
Grimsley 3.605
Solomon 3.884
Lehninger 3.846
Nozaki 4.012
DTASelect 4.24
Thurlkill 3.745
EMBOSS 3.834
Sillero 4.024
Patrickios 1.914
IPC_peptide 3.884
IPC2_peptide 4.012
IPC2.peptide.svr19 3.926
Protein with the highest isoelectric point:
>tr|A0A6P2BRK4|A0A6P2BRK4_9ACTN ABC transporter permease OS=Trebonia kvetii OX=2480626 GN=EAS64_34075 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.99 KK16 pKa = 9.77 HH17 pKa = 5.81 RR18 pKa = 11.84 KK19 pKa = 8.55 LLKK22 pKa = 8.44 KK23 pKa = 9.17 TRR25 pKa = 11.84 IQRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.73 KK32 pKa = 9.84
Molecular weight: 4.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.473
IPC2_protein 11.082
IPC_protein 12.618
Toseland 12.808
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.793
Wikipedia 13.276
Rodwell 12.735
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.808
DTASelect 12.793
Thurlkill 12.808
EMBOSS 13.29
Sillero 12.808
Patrickios 12.457
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.03
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8100
0
8100
2589830
25
2662
319.7
34.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.406 ± 0.041
0.839 ± 0.009
5.544 ± 0.023
4.916 ± 0.023
2.922 ± 0.016
9.587 ± 0.029
2.075 ± 0.012
3.951 ± 0.018
1.932 ± 0.017
10.035 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.867 ± 0.011
1.983 ± 0.015
6.052 ± 0.025
2.991 ± 0.017
7.543 ± 0.027
5.584 ± 0.025
5.895 ± 0.025
8.084 ± 0.022
1.593 ± 0.011
2.202 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here