Podarcis muralis (Wall lizard) (Lacerta muralis)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Lepi

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 36445 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A670JAM0|A0A670JAM0_PODMU Reverse transcriptase domain-containing protein OS=Podarcis muralis OX=64176 PE=4 SV=1
MM1 pKa = 7.5WPCPLLCCLSPLLFLCLLFLPSGHH25 pKa = 7.2LCPSVCSCMDD35 pKa = 3.26YY36 pKa = 8.82HH37 pKa = 7.22TIDD40 pKa = 4.7CRR42 pKa = 11.84DD43 pKa = 3.12QGLPRR48 pKa = 11.84VPSPFPLDD56 pKa = 3.48VRR58 pKa = 11.84KK59 pKa = 9.76LLIADD64 pKa = 3.87NNIQEE69 pKa = 4.19IPGDD73 pKa = 3.61FFIFYY78 pKa = 10.58SDD80 pKa = 4.06LVYY83 pKa = 11.04LDD85 pKa = 4.07FRR87 pKa = 11.84NNSIATLQDD96 pKa = 3.16GTFSSSSKK104 pKa = 10.51LVYY107 pKa = 10.55LDD109 pKa = 4.63LSYY112 pKa = 11.96NNLTQLDD119 pKa = 3.58AGIFKK124 pKa = 10.62SAEE127 pKa = 3.94KK128 pKa = 10.38LIKK131 pKa = 10.55LSLGNNNLAEE141 pKa = 4.06VDD143 pKa = 3.65EE144 pKa = 4.83AAFEE148 pKa = 4.43SLKK151 pKa = 10.37QLQVLEE157 pKa = 5.01LNDD160 pKa = 4.57NNLQTLNVATFDD172 pKa = 3.74ALPNLRR178 pKa = 11.84TIRR181 pKa = 11.84LEE183 pKa = 4.19GNPWLCDD190 pKa = 3.4CDD192 pKa = 4.08FASLFSWLEE201 pKa = 3.55ANAPKK206 pKa = 10.16LQKK209 pKa = 10.87GLDD212 pKa = 4.03EE213 pKa = 4.48IQCTVPVEE221 pKa = 4.34EE222 pKa = 3.98ITIFLSEE229 pKa = 4.07LSEE232 pKa = 4.67ASFRR236 pKa = 11.84DD237 pKa = 3.87CKK239 pKa = 10.78FSLSLTDD246 pKa = 4.77LIIIIFSGVAVSIAAIVSSFVLALIVNCFQRR277 pKa = 11.84CAPSKK282 pKa = 11.06DD283 pKa = 3.52DD284 pKa = 6.59DD285 pKa = 4.48EE286 pKa = 7.61DD287 pKa = 4.99EE288 pKa = 5.98DD289 pKa = 6.53DD290 pKa = 5.86DD291 pKa = 5.34DD292 pKa = 4.57

Molecular weight: 32.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A670KF72|A0A670KF72_PODMU KRAB domain-containing protein OS=Podarcis muralis OX=64176 PE=4 SV=1
KKK2 pKa = 9.19KKK4 pKa = 10.14RR5 pKa = 11.84VAARR9 pKa = 11.84KKK11 pKa = 7.45KKK13 pKa = 9.95RR14 pKa = 11.84VAARR18 pKa = 11.84KKK20 pKa = 7.35KKK22 pKa = 9.9RR23 pKa = 11.84VSARR27 pKa = 11.84KKK29 pKa = 8.37KKK31 pKa = 10.01RR32 pKa = 11.84VSARR36 pKa = 11.84KKK38 pKa = 8.52KKK40 pKa = 10.04RR41 pKa = 11.84VAARR45 pKa = 11.84KKK47 pKa = 7.45KKK49 pKa = 9.95RR50 pKa = 11.84VAARR54 pKa = 11.84KKK56 pKa = 7.35KKK58 pKa = 9.9RR59 pKa = 11.84VSARR63 pKa = 11.84KKK65 pKa = 8.52KKK67 pKa = 10.04RR68 pKa = 11.84VAARR72 pKa = 11.84KKK74 pKa = 7.45KKK76 pKa = 9.95RR77 pKa = 11.84VAARR81 pKa = 11.84KKK83 pKa = 7.35KKK85 pKa = 9.9RR86 pKa = 11.84VSARR90 pKa = 11.84KKK92 pKa = 8.52KKK94 pKa = 10.04RR95 pKa = 11.84VAARR99 pKa = 11.84KKK101 pKa = 7.45KKK103 pKa = 9.95RR104 pKa = 11.84VAARR108 pKa = 11.84KKK110 pKa = 7.35KKK112 pKa = 9.9RR113 pKa = 11.84VSARR117 pKa = 11.84KKK119 pKa = 8.52KKK121 pKa = 10.04RR122 pKa = 11.84VAARR126 pKa = 11.84KKK128 pKa = 7.45KKK130 pKa = 9.95RR131 pKa = 11.84VAARR135 pKa = 11.84KKK137 pKa = 7.35KKK139 pKa = 9.9RR140 pKa = 11.84VSARR144 pKa = 11.84KKK146 pKa = 8.52KKK148 pKa = 10.04RR149 pKa = 11.84VAARR153 pKa = 11.84KKK155 pKa = 7.45KKK157 pKa = 9.95RR158 pKa = 11.84VAARR162 pKa = 11.84KKK164 pKa = 7.35KKK166 pKa = 9.9RR167 pKa = 11.84VSARR171 pKa = 11.84KKK173 pKa = 8.66KKK175 pKa = 9.76QYYY178 pKa = 10.11FKKK181 pKa = 10.61NPSVGPQL

Molecular weight: 21.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21928

14517

36445

24682227

22

7046

677.2

75.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.69 ± 0.012

2.309 ± 0.01

4.898 ± 0.008

7.111 ± 0.014

3.876 ± 0.009

6.138 ± 0.014

2.588 ± 0.006

4.852 ± 0.009

6.188 ± 0.015

9.867 ± 0.015

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.267 ± 0.004

3.981 ± 0.009

5.516 ± 0.015

4.676 ± 0.011

5.472 ± 0.01

8.122 ± 0.015

5.296 ± 0.008

6.05 ± 0.009

1.266 ± 0.004

2.834 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski