Bacillus virus G
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 675 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G3MAY7|G3MAY7_9CAUD Gp607 OS=Bacillus virus G OX=1084719 GN=607 PE=4 SV=1
MM1 pKa = 7.13 SQLTEE6 pKa = 3.9 EE7 pKa = 4.32 QIEE10 pKa = 4.39 EE11 pKa = 4.06 VVEE14 pKa = 4.84 AIFDD18 pKa = 3.85 MVPEE22 pKa = 4.69 DD23 pKa = 4.15 LCGMGYY29 pKa = 10.03 FGEE32 pKa = 4.61 AIKK35 pKa = 11.07 HH36 pKa = 5.94 NGLEE40 pKa = 4.06 HH41 pKa = 7.31 LFTEE45 pKa = 4.47 EE46 pKa = 4.19 QMNTFHH52 pKa = 7.29 EE53 pKa = 4.49 CQIVYY58 pKa = 10.38 GPLGSDD64 pKa = 3.31 EE65 pKa = 4.52 EE66 pKa = 4.46 EE67 pKa = 4.43 EE68 pKa = 4.23 EE69 pKa = 5.59 NEE71 pKa = 5.32 DD72 pKa = 4.33 EE73 pKa = 6.21 DD74 pKa = 6.63 DD75 pKa = 4.44 DD76 pKa = 6.79 GEE78 pKa = 5.67 DD79 pKa = 3.31 NGCDD83 pKa = 3.32 YY84 pKa = 10.76 EE85 pKa = 4.63 VRR87 pKa = 11.84 HH88 pKa = 6.2 SYY90 pKa = 10.45 PWMSGSWYY98 pKa = 9.98 DD99 pKa = 3.67 YY100 pKa = 11.22 EE101 pKa = 4.26 EE102 pKa = 4.69 SLDD105 pKa = 3.69 EE106 pKa = 4.81 RR107 pKa = 11.84 IKK109 pKa = 11.18 EE110 pKa = 4.16 EE111 pKa = 3.4 ITTVVKK117 pKa = 10.38 VVIKK121 pKa = 10.42 NVKK124 pKa = 9.14
Molecular weight: 14.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.762
IPC2_protein 3.935
IPC_protein 3.872
Toseland 3.694
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.681
Rodwell 3.706
Grimsley 3.617
Solomon 3.808
Lehninger 3.757
Nozaki 3.935
DTASelect 4.05
Thurlkill 3.719
EMBOSS 3.706
Sillero 3.973
Patrickios 1.062
IPC_peptide 3.808
IPC2_peptide 3.961
IPC2.peptide.svr19 3.87
Protein with the highest isoelectric point:
>tr|G3MAJ1|G3MAJ1_9CAUD Gp450 OS=Bacillus virus G OX=1084719 GN=450 PE=4 SV=1
MM1 pKa = 7.71 YY2 pKa = 8.33 DD3 pKa = 3.83 TIVLSIFFVIVVPMIFLLLLSRR25 pKa = 11.84 GKK27 pKa = 10.53 VEE29 pKa = 4.43 MKK31 pKa = 10.34 CLKK34 pKa = 10.07 CKK36 pKa = 9.61 KK37 pKa = 8.73 TFTQFRR43 pKa = 11.84 VRR45 pKa = 11.84 SHH47 pKa = 6.0 SYY49 pKa = 10.41 YY50 pKa = 10.09 YY51 pKa = 9.54 EE52 pKa = 3.9 QRR54 pKa = 11.84 CPHH57 pKa = 6.11 CNYY60 pKa = 9.95 RR61 pKa = 11.84 YY62 pKa = 8.62 STMRR66 pKa = 11.84 RR67 pKa = 11.84 KK68 pKa = 9.94 KK69 pKa = 9.25 VTLKK73 pKa = 10.67 RR74 pKa = 3.65
Molecular weight: 9.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.305
IPC2_protein 9.736
IPC_protein 10.028
Toseland 10.233
ProMoST 10.072
Dawson 10.438
Bjellqvist 10.145
Wikipedia 10.613
Rodwell 10.847
Grimsley 10.511
Solomon 10.467
Lehninger 10.438
Nozaki 10.277
DTASelect 10.131
Thurlkill 10.292
EMBOSS 10.643
Sillero 10.365
Patrickios 10.526
IPC_peptide 10.467
IPC2_peptide 9.209
IPC2.peptide.svr19 8.492
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
675
0
675
144966
18
2893
214.8
24.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.594 ± 0.129
0.928 ± 0.044
6.359 ± 0.077
8.367 ± 0.176
4.585 ± 0.08
5.322 ± 0.14
1.671 ± 0.05
8.256 ± 0.121
9.108 ± 0.136
8.305 ± 0.098
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.547 ± 0.068
6.805 ± 0.123
2.615 ± 0.082
3.146 ± 0.069
3.715 ± 0.075
6.841 ± 0.15
5.201 ± 0.116
6.12 ± 0.096
1.029 ± 0.037
4.484 ± 0.087
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here