Dichotomicrobium thermohalophilum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Hyphomicrobiaceae; Dichotomicrobium

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2737 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A397Q2Q8|A0A397Q2Q8_9RHIZ Malate dehydrogenase (Oxaloacetate-decarboxylating)(NADP+) OS=Dichotomicrobium thermohalophilum OX=933063 GN=BXY53_0399 PE=3 SV=1
MM1 pKa = 6.74YY2 pKa = 9.46TFTKK6 pKa = 9.15YY7 pKa = 11.36ALMPLMLAAFMTPLNAQTSVYY28 pKa = 9.9QDD30 pKa = 3.45PEE32 pKa = 4.08RR33 pKa = 11.84CVAEE37 pKa = 4.47MSALDD42 pKa = 3.96ADD44 pKa = 4.14GDD46 pKa = 4.37GYY48 pKa = 10.33LTDD51 pKa = 5.07DD52 pKa = 3.83EE53 pKa = 4.78VGEE56 pKa = 4.25YY57 pKa = 7.29MTISTRR63 pKa = 11.84VDD65 pKa = 2.8TDD67 pKa = 3.1GDD69 pKa = 3.8GRR71 pKa = 11.84ISSEE75 pKa = 4.08EE76 pKa = 3.8LVVACEE82 pKa = 4.02QQLVEE87 pKa = 4.25ALEE90 pKa = 4.49GSGRR94 pKa = 3.6

Molecular weight: 10.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A397PFL5|A0A397PFL5_9RHIZ Regulator of CtrA degradation OS=Dichotomicrobium thermohalophilum OX=933063 GN=BXY53_2380 PE=4 SV=1
MM1 pKa = 7.31KK2 pKa = 10.33RR3 pKa = 11.84SFQPSKK9 pKa = 10.27LRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 9.14RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.66GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.2AGQKK29 pKa = 8.92ILARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.61QLTAA44 pKa = 3.78

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2737

0

2737

885794

29

1750

323.6

35.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.308 ± 0.072

0.864 ± 0.016

5.831 ± 0.039

6.721 ± 0.043

3.655 ± 0.029

8.392 ± 0.043

2.067 ± 0.021

5.101 ± 0.034

3.059 ± 0.036

9.892 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.468 ± 0.022

2.511 ± 0.023

5.327 ± 0.036

3.291 ± 0.025

7.484 ± 0.052

4.976 ± 0.03

5.108 ± 0.027

7.248 ± 0.037

1.358 ± 0.017

2.34 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski