Paraburkholderia panacisoli

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Paraburkholderia

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9325 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B0HBR3|A0A5B0HBR3_9BURK Uncharacterized protein OS=Paraburkholderia panacisoli OX=2603818 GN=FVF58_10970 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84ISSSQYY8 pKa = 8.87FSMNVEE14 pKa = 4.4TMSSQQAQLSQIYY27 pKa = 7.71QQISSGVSLSTAADD41 pKa = 3.72NPLGAAQAVQLSSTATALSQYY62 pKa = 9.58TSNQGAALSSLQAEE76 pKa = 4.79DD77 pKa = 3.68STLSGVLSTLQSVNTQALRR96 pKa = 11.84ASDD99 pKa = 4.16GSLNDD104 pKa = 3.75ADD106 pKa = 4.35RR107 pKa = 11.84SAIATQLTSLRR118 pKa = 11.84DD119 pKa = 3.56QLLTYY124 pKa = 11.26ANATDD129 pKa = 3.6SAGNSLFSGFQNATQSFTTNASGAVVYY156 pKa = 10.96SGDD159 pKa = 3.3TGTRR163 pKa = 11.84TVQVTDD169 pKa = 3.59SNQVATGDD177 pKa = 3.34NGIAVFMSVGAVNAQPVASGSSGNLGTGVIGAVTVNDD214 pKa = 3.67PTNAANADD222 pKa = 3.68SYY224 pKa = 11.33QIQFSGSGASLTYY237 pKa = 10.2QINDD241 pKa = 3.41TTTGVAGTAQAYY253 pKa = 6.32TAGANITIAGQTVAITGTPSAGDD276 pKa = 3.53TFTVQPAKK284 pKa = 10.42QAGTDD289 pKa = 3.29VFANLNAMISALQQPVAGNAAAAATLQNAMNTGLTQLANTMSNVTAVQASVGGRR343 pKa = 11.84EE344 pKa = 4.05QEE346 pKa = 4.26VKK348 pKa = 10.66ALQTVTEE355 pKa = 4.44TNSLQAQSNLSDD367 pKa = 3.57LTSTDD372 pKa = 3.14MVTTISKK379 pKa = 8.59YY380 pKa = 8.8TMQQAALQASQQAFVQVQNMSLFQYY405 pKa = 10.64INNN408 pKa = 3.99

Molecular weight: 41.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B0GSL7|A0A5B0GSL7_9BURK Hsp70 family protein OS=Paraburkholderia panacisoli OX=2603818 GN=FVF58_28105 PE=4 SV=1
MM1 pKa = 7.49AATVAKK7 pKa = 10.38LRR9 pKa = 11.84LSPAGLSRR17 pKa = 11.84GHH19 pKa = 5.85HH20 pKa = 4.96WRR22 pKa = 11.84WRR24 pKa = 11.84LRR26 pKa = 11.84GAIQLAPSSSPTTSTRR42 pKa = 11.84LRR44 pKa = 11.84IPTRR48 pKa = 11.84NSAAARR54 pKa = 11.84INFSKK59 pKa = 10.53KK60 pKa = 9.18GALGKK65 pKa = 10.09LVPTGFF71 pKa = 3.85

Molecular weight: 7.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9325

0

9325

2853921

20

2871

306.1

33.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.579 ± 0.036

0.969 ± 0.008

5.333 ± 0.02

5.146 ± 0.026

3.743 ± 0.016

8.104 ± 0.021

2.359 ± 0.012

4.773 ± 0.019

3.103 ± 0.022

10.225 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.389 ± 0.012

2.824 ± 0.015

5.036 ± 0.019

3.639 ± 0.017

6.97 ± 0.025

5.824 ± 0.02

5.436 ± 0.02

7.739 ± 0.021

1.386 ± 0.01

2.423 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski