Pseudogulbenkiania sp. (strain NH8B)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Chromobacteriaceae; Pseudogulbenkiania; unclassified Pseudogulbenkiania

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3958 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G2IWY2|G2IWY2_PSEUL 2-hydroxy-3-oxopropionate reductase OS=Pseudogulbenkiania sp. (strain NH8B) OX=748280 GN=NH8B_0496 PE=4 SV=1
MM1 pKa = 7.87ASTTTSGIITSFSNTPQAGDD21 pKa = 3.93DD22 pKa = 4.28LFTSSSTGLTEE33 pKa = 4.53DD34 pKa = 3.44SSSIVYY40 pKa = 10.08LNVMANDD47 pKa = 3.87LGGAAKK53 pKa = 9.36TLYY56 pKa = 10.85SVDD59 pKa = 4.57DD60 pKa = 4.11GTNSAGITSSTDD72 pKa = 3.37LLTKK76 pKa = 9.87DD77 pKa = 3.2TAYY80 pKa = 9.77STDD83 pKa = 3.24VSAHH87 pKa = 5.84GARR90 pKa = 11.84IWITTDD96 pKa = 2.48GRR98 pKa = 11.84VGYY101 pKa = 9.65DD102 pKa = 3.17ASTLSADD109 pKa = 5.21FKK111 pKa = 10.98ATLNALGAGEE121 pKa = 4.33YY122 pKa = 8.52QTDD125 pKa = 3.48TFTYY129 pKa = 10.19AIRR132 pKa = 11.84LGNGTLSWATATVQFAGVNDD152 pKa = 3.83AATFSGDD159 pKa = 3.36DD160 pKa = 3.56TKK162 pKa = 11.76GLTEE166 pKa = 3.9TDD168 pKa = 3.16AAQTTGGTLVAHH180 pKa = 6.83DD181 pKa = 4.2VDD183 pKa = 4.43SAEE186 pKa = 4.22TFQAQSNVAGSSGYY200 pKa = 10.14GHH202 pKa = 6.25FTIGSDD208 pKa = 3.86GTWSYY213 pKa = 11.28TMDD216 pKa = 3.53SAHH219 pKa = 6.83NEE221 pKa = 3.96FKK223 pKa = 11.14DD224 pKa = 3.51GVNYY228 pKa = 10.75SDD230 pKa = 3.49TLVVKK235 pKa = 10.54SADD238 pKa = 3.8GTEE241 pKa = 3.95HH242 pKa = 6.63TLTVNIAGTNDD253 pKa = 3.04AATFSGDD260 pKa = 3.38DD261 pKa = 3.64SKK263 pKa = 12.03GLTEE267 pKa = 3.9TDD269 pKa = 3.31AAQSTGGTLVAHH281 pKa = 6.83DD282 pKa = 4.2VDD284 pKa = 4.43SAEE287 pKa = 4.22TFQAQTNVAGSNGYY301 pKa = 8.51GHH303 pKa = 6.27FTIGSDD309 pKa = 3.86GTWSYY314 pKa = 11.28TMDD317 pKa = 3.53SAHH320 pKa = 6.9NEE322 pKa = 4.08FKK324 pKa = 10.97DD325 pKa = 3.42GEE327 pKa = 4.48NYY329 pKa = 10.63SDD331 pKa = 3.55TLVVQSADD339 pKa = 3.58GTEE342 pKa = 3.96HH343 pKa = 6.66TLTVNITGTNDD354 pKa = 2.93AATFTGDD361 pKa = 3.4DD362 pKa = 3.88SKK364 pKa = 11.97GLTEE368 pKa = 4.07TDD370 pKa = 3.03AAQTTGGTLVAHH382 pKa = 6.83DD383 pKa = 4.2VDD385 pKa = 4.43SAEE388 pKa = 4.22TFQAQTNVAGSNGYY402 pKa = 8.51GHH404 pKa = 6.27FTIGSDD410 pKa = 3.86GTWSYY415 pKa = 11.28TMDD418 pKa = 3.52SAHH421 pKa = 6.67NEE423 pKa = 3.84FAAGTTYY430 pKa = 11.33SDD432 pKa = 3.52TLVVQSADD440 pKa = 3.58GTEE443 pKa = 3.96HH444 pKa = 6.61TLTVNILGTNDD455 pKa = 3.14AATFTGDD462 pKa = 3.4DD463 pKa = 3.88SKK465 pKa = 11.97GLTEE469 pKa = 4.07TDD471 pKa = 3.03AAQTTGGTLVAHH483 pKa = 6.83DD484 pKa = 4.2VDD486 pKa = 4.43SAEE489 pKa = 4.22TFQAQSNVAGSNGYY503 pKa = 8.51GHH505 pKa = 6.27FTIGSDD511 pKa = 3.86GTWSYY516 pKa = 11.28TMDD519 pKa = 3.53SAHH522 pKa = 6.83NEE524 pKa = 3.96FKK526 pKa = 11.14DD527 pKa = 3.51GVNYY531 pKa = 10.75SDD533 pKa = 3.49TLVVKK538 pKa = 10.54SADD541 pKa = 3.8GTEE544 pKa = 3.95HH545 pKa = 6.68TLTVNITGTNDD556 pKa = 2.92AATFSGDD563 pKa = 3.38DD564 pKa = 3.64SKK566 pKa = 12.03GLTEE570 pKa = 3.9TDD572 pKa = 3.31AAQSTGGTLVAHH584 pKa = 6.83DD585 pKa = 4.2VDD587 pKa = 4.43SAEE590 pKa = 4.22TFQAQTNVAGSQGYY604 pKa = 7.93GHH606 pKa = 6.24FTIGSDD612 pKa = 4.0GAWSYY617 pKa = 11.29TMDD620 pKa = 3.6SAHH623 pKa = 6.83NEE625 pKa = 3.96FKK627 pKa = 11.11DD628 pKa = 3.57GVNYY632 pKa = 10.36TDD634 pKa = 3.48TLVVKK639 pKa = 10.48SADD642 pKa = 3.8GTEE645 pKa = 3.95HH646 pKa = 6.68TLTVNITGTNDD657 pKa = 2.92AATFSGDD664 pKa = 3.36DD665 pKa = 3.56TKK667 pKa = 11.76GLTEE671 pKa = 3.9TDD673 pKa = 3.16AAQTTGGTLVAHH685 pKa = 6.83DD686 pKa = 4.2VDD688 pKa = 4.43SAEE691 pKa = 4.22TFQAQTNVAGSQGYY705 pKa = 7.93GHH707 pKa = 6.24FTIGSDD713 pKa = 3.94GTWSYY718 pKa = 12.25SMDD721 pKa = 3.58SAHH724 pKa = 7.65DD725 pKa = 3.72EE726 pKa = 4.41FAGGTTNTDD735 pKa = 3.37TLVVQSADD743 pKa = 3.58GTEE746 pKa = 3.96HH747 pKa = 6.66TLTVNITGTNDD758 pKa = 3.03AATITASASEE768 pKa = 4.33DD769 pKa = 3.54TSVTEE774 pKa = 4.04VGSGVAGDD782 pKa = 4.15SSAGGQLTVHH792 pKa = 6.78DD793 pKa = 4.34VDD795 pKa = 3.46NGEE798 pKa = 4.39AHH800 pKa = 6.26FQTPVSLTGTYY811 pKa = 11.12GDD813 pKa = 4.1FSFNASTGVWSYY825 pKa = 12.0ALDD828 pKa = 3.78DD829 pKa = 4.05TRR831 pKa = 11.84PATDD835 pKa = 3.44ALATGSVVHH844 pKa = 6.69DD845 pKa = 3.72TLTVKK850 pKa = 10.72SFDD853 pKa = 3.39GSASYY858 pKa = 9.93TIDD861 pKa = 3.34VAVNGSNDD869 pKa = 3.9SVNHH873 pKa = 6.34APTDD877 pKa = 3.51IVLSMAEE884 pKa = 4.09PGGNALPGAGAVLGTLSTIDD904 pKa = 3.62VDD906 pKa = 4.32SGDD909 pKa = 3.55TFTYY913 pKa = 10.9SLISSSTVSGSAASFSISGNSLSTGTGLASNSVYY947 pKa = 10.62QLDD950 pKa = 3.69IQTTDD955 pKa = 3.14SASATYY961 pKa = 10.43HH962 pKa = 5.36EE963 pKa = 5.1VFNIITGTNAQGQVTGDD980 pKa = 4.03DD981 pKa = 3.78GSLPSGGGANSILIGDD997 pKa = 4.03DD998 pKa = 3.26VLYY1001 pKa = 10.99GSGGNDD1007 pKa = 3.05VMFGGSGNDD1016 pKa = 3.31TLFGQNGNDD1025 pKa = 3.43VLYY1028 pKa = 10.92GGAGNDD1034 pKa = 3.65TLSGGNQADD1043 pKa = 3.37RR1044 pKa = 11.84FVFLASDD1051 pKa = 3.32NGGTDD1056 pKa = 3.64TITNFTATGANADD1069 pKa = 4.01TLDD1072 pKa = 4.77ISDD1075 pKa = 5.32LLINYY1080 pKa = 8.58NPATPSAFVHH1090 pKa = 5.75FTEE1093 pKa = 5.14SGGNTIVSVDD1103 pKa = 3.47RR1104 pKa = 11.84DD1105 pKa = 3.83GSGGSYY1111 pKa = 10.43GFQDD1115 pKa = 3.47VAILNGVTGLNMNDD1129 pKa = 3.23MLTSGKK1135 pKa = 10.29LDD1137 pKa = 3.45VVPP1140 pKa = 5.06

Molecular weight: 116.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G2J349|G2J349_PSEUL Uncharacterized protein OS=Pseudogulbenkiania sp. (strain NH8B) OX=748280 GN=NH8B_1677 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.31QPSTTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.24RR14 pKa = 11.84THH16 pKa = 5.92GFLVRR21 pKa = 11.84SKK23 pKa = 9.38TRR25 pKa = 11.84GGRR28 pKa = 11.84AVLAARR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.45GRR39 pKa = 11.84KK40 pKa = 8.54RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3958

0

3958

1248374

40

1991

315.4

34.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.907 ± 0.052

0.973 ± 0.013

5.251 ± 0.026

5.717 ± 0.04

3.572 ± 0.024

8.077 ± 0.036

2.367 ± 0.02

4.58 ± 0.031

3.492 ± 0.036

11.533 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.372 ± 0.016

2.738 ± 0.025

5.012 ± 0.027

4.089 ± 0.031

6.659 ± 0.043

5.442 ± 0.031

4.909 ± 0.034

7.398 ± 0.033

1.423 ± 0.02

2.489 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski