Amino acid dipepetide frequency for Pseudogulbenkiania sp. (strain NH8B)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.613AlaAla: 15.613 ± 0.185	1.262AlaCys: 1.262 ± 0.032	6.14AlaAsp: 6.14 ± 0.068	7.824AlaGlu: 7.824 ± 0.101	3.812AlaPhe: 3.812 ± 0.059	10.194AlaGly: 10.194 ± 0.103	2.414AlaHis: 2.414 ± 0.05	5.44AlaIle: 5.44 ± 0.072	3.924AlaLys: 3.924 ± 0.059	14.293AlaLeu: 14.293 ± 0.157	3.339AlaMet: 3.339 ± 0.05	2.965AlaAsn: 2.965 ± 0.056	5.079AlaPro: 5.079 ± 0.084	4.506AlaGln: 4.506 ± 0.08	8.209AlaArg: 8.209 ± 0.102	6.23AlaSer: 6.23 ± 0.083	5.321AlaThr: 5.321 ± 0.074	8.37AlaVal: 8.37 ± 0.088	1.664AlaTrp: 1.664 ± 0.045	2.474AlaTyr: 2.474 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	1.006CysAla: 1.006 ± 0.036	0.155CysCys: 0.155 ± 0.012	0.535CysAsp: 0.535 ± 0.022	0.511CysGlu: 0.511 ± 0.021	0.33CysPhe: 0.33 ± 0.017	0.994CysGly: 0.994 ± 0.031	0.328CysHis: 0.328 ± 0.017	0.377CysIle: 0.377 ± 0.019	0.262CysLys: 0.262 ± 0.016	0.993CysLeu: 0.993 ± 0.03	0.177CysMet: 0.177 ± 0.012	0.26CysAsn: 0.26 ± 0.016	0.56CysPro: 0.56 ± 0.023	0.407CysGln: 0.407 ± 0.018	0.736CysArg: 0.736 ± 0.025	0.573CysSer: 0.573 ± 0.023	0.451CysThr: 0.451 ± 0.018	0.636CysVal: 0.636 ± 0.024	0.148CysTrp: 0.148 ± 0.01	0.294CysTyr: 0.294 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	6.227AspAla: 6.227 ± 0.077	0.488AspCys: 0.488 ± 0.018	2.929AspAsp: 2.929 ± 0.065	3.339AspGlu: 3.339 ± 0.061	2.052AspPhe: 2.052 ± 0.046	4.756AspGly: 4.756 ± 0.082	1.111AspHis: 1.111 ± 0.03	2.739AspIle: 2.739 ± 0.046	2.087AspLys: 2.087 ± 0.045	5.016AspLeu: 5.016 ± 0.059	1.245AspMet: 1.245 ± 0.028	1.464AspAsn: 1.464 ± 0.039	2.675AspPro: 2.675 ± 0.048	1.657AspGln: 1.657 ± 0.033	2.895AspArg: 2.895 ± 0.052	2.652AspSer: 2.652 ± 0.059	2.711AspThr: 2.711 ± 0.053	3.764AspVal: 3.764 ± 0.058	1.025AspTrp: 1.025 ± 0.028	1.681AspTyr: 1.681 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	7.149GluAla: 7.149 ± 0.094	0.465GluCys: 0.465 ± 0.019	2.285GluAsp: 2.285 ± 0.046	3.086GluGlu: 3.086 ± 0.052	1.816GluPhe: 1.816 ± 0.043	3.699GluGly: 3.699 ± 0.063	1.614GluHis: 1.614 ± 0.039	2.857GluIle: 2.857 ± 0.053	2.051GluLys: 2.051 ± 0.04	6.996GluLeu: 6.996 ± 0.092	1.319GluMet: 1.319 ± 0.036	1.415GluAsn: 1.415 ± 0.038	2.549GluPro: 2.549 ± 0.05	3.145GluGln: 3.145 ± 0.055	4.918GluArg: 4.918 ± 0.078	2.655GluSer: 2.655 ± 0.048	2.772GluThr: 2.772 ± 0.045	4.385GluVal: 4.385 ± 0.068	0.821GluTrp: 0.821 ± 0.026	1.176GluTyr: 1.176 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	4.195PheAla: 4.195 ± 0.065	0.414PheCys: 0.414 ± 0.019	2.442PheAsp: 2.442 ± 0.048	1.996PheGlu: 1.996 ± 0.038	1.396PhePhe: 1.396 ± 0.04	3.336PheGly: 3.336 ± 0.057	0.792PheHis: 0.792 ± 0.027	1.653PheIle: 1.653 ± 0.035	1.227PheLys: 1.227 ± 0.034	3.352PheLeu: 3.352 ± 0.057	0.816PheMet: 0.816 ± 0.024	1.153PheAsn: 1.153 ± 0.034	1.509PhePro: 1.509 ± 0.038	1.109PheGln: 1.109 ± 0.03	1.986PheArg: 1.986 ± 0.041	2.231PheSer: 2.231 ± 0.045	1.875PheThr: 1.875 ± 0.043	2.702PheVal: 2.702 ± 0.052	0.532PheTrp: 0.532 ± 0.023	1.007PheTyr: 1.007 ± 0.031	0.0PheXaa: 0.0 ± 0.0
Gly	7.845GlyAla: 7.845 ± 0.08	0.952GlyCys: 0.952 ± 0.03	3.884GlyAsp: 3.884 ± 0.069	4.944GlyGlu: 4.944 ± 0.069	3.352GlyPhe: 3.352 ± 0.055	6.47GlyGly: 6.47 ± 0.09	1.99GlyHis: 1.99 ± 0.045	4.111GlyIle: 4.111 ± 0.055	3.683GlyLys: 3.683 ± 0.06	8.895GlyLeu: 8.895 ± 0.095	2.342GlyMet: 2.342 ± 0.045	2.215GlyAsn: 2.215 ± 0.047	2.439GlyPro: 2.439 ± 0.041	3.513GlyGln: 3.513 ± 0.065	5.276GlyArg: 5.276 ± 0.068	4.478GlySer: 4.478 ± 0.062	3.874GlyThr: 3.874 ± 0.074	6.531GlyVal: 6.531 ± 0.082	1.469GlyTrp: 1.469 ± 0.034	2.502GlyTyr: 2.502 ± 0.043	0.0GlyXaa: 0.0 ± 0.0
His	2.591HisAla: 2.591 ± 0.047	0.302HisCys: 0.302 ± 0.017	1.418HisAsp: 1.418 ± 0.032	1.276HisGlu: 1.276 ± 0.035	1.002HisPhe: 1.002 ± 0.028	2.254HisGly: 2.254 ± 0.045	0.743HisHis: 0.743 ± 0.028	1.026HisIle: 1.026 ± 0.028	0.642HisLys: 0.642 ± 0.024	2.359HisLeu: 2.359 ± 0.042	0.498HisMet: 0.498 ± 0.02	0.584HisAsn: 0.584 ± 0.022	1.617HisPro: 1.617 ± 0.041	0.872HisGln: 0.872 ± 0.025	1.435HisArg: 1.435 ± 0.036	1.186HisSer: 1.186 ± 0.031	1.065HisThr: 1.065 ± 0.028	1.563HisVal: 1.563 ± 0.037	0.435HisTrp: 0.435 ± 0.018	0.803HisTyr: 0.803 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	5.855IleAla: 5.855 ± 0.074	0.424IleCys: 0.424 ± 0.019	3.207IleAsp: 3.207 ± 0.053	3.114IleGlu: 3.114 ± 0.052	1.253IlePhe: 1.253 ± 0.033	4.433IleGly: 4.433 ± 0.069	0.98IleHis: 0.98 ± 0.028	1.832IleIle: 1.832 ± 0.044	1.745IleLys: 1.745 ± 0.039	3.827IleLeu: 3.827 ± 0.055	0.864IleMet: 0.864 ± 0.028	1.556IleAsn: 1.556 ± 0.044	2.171IlePro: 2.171 ± 0.044	1.459IleGln: 1.459 ± 0.037	2.921IleArg: 2.921 ± 0.051	2.539IleSer: 2.539 ± 0.051	2.457IleThr: 2.457 ± 0.05	3.594IleVal: 3.594 ± 0.05	0.475IleTrp: 0.475 ± 0.018	1.093IleTyr: 1.093 ± 0.034	0.0IleXaa: 0.0 ± 0.0
Lys	3.964LysAla: 3.964 ± 0.074	0.175LysCys: 0.175 ± 0.015	1.664LysAsp: 1.664 ± 0.04	1.774LysGlu: 1.774 ± 0.046	0.876LysPhe: 0.876 ± 0.032	2.603LysGly: 2.603 ± 0.062	0.768LysHis: 0.768 ± 0.023	1.66LysIle: 1.66 ± 0.041	1.579LysLys: 1.579 ± 0.046	4.016LysLeu: 4.016 ± 0.062	0.879LysMet: 0.879 ± 0.026	1.145LysAsn: 1.145 ± 0.032	2.063LysPro: 2.063 ± 0.046	1.582LysGln: 1.582 ± 0.041	2.28LysArg: 2.28 ± 0.04	1.974LysSer: 1.974 ± 0.041	1.983LysThr: 1.983 ± 0.04	2.792LysVal: 2.792 ± 0.049	0.368LysTrp: 0.368 ± 0.018	0.772LysTyr: 0.772 ± 0.028	0.0LysXaa: 0.0 ± 0.0
Leu	16.398LeuAla: 16.398 ± 0.172	1.109LeuCys: 1.109 ± 0.031	6.412LeuAsp: 6.412 ± 0.085	6.046LeuGlu: 6.046 ± 0.086	4.084LeuPhe: 4.084 ± 0.063	8.708LeuGly: 8.708 ± 0.097	2.376LeuHis: 2.376 ± 0.046	5.036LeuIle: 5.036 ± 0.072	4.244LeuLys: 4.244 ± 0.057	12.918LeuLeu: 12.918 ± 0.177	2.586LeuMet: 2.586 ± 0.051	3.102LeuAsn: 3.102 ± 0.05	6.564LeuPro: 6.564 ± 0.08	4.191LeuGln: 4.191 ± 0.072	6.779LeuArg: 6.779 ± 0.085	7.052LeuSer: 7.052 ± 0.083	6.146LeuThr: 6.146 ± 0.079	7.715LeuVal: 7.715 ± 0.092	1.375LeuTrp: 1.375 ± 0.039	2.484LeuTyr: 2.484 ± 0.045	0.0LeuXaa: 0.0 ± 0.0
Met	2.901MetAla: 2.901 ± 0.049	0.147MetCys: 0.147 ± 0.01	1.069MetAsp: 1.069 ± 0.03	1.157MetGlu: 1.157 ± 0.029	0.685MetPhe: 0.685 ± 0.024	1.744MetGly: 1.744 ± 0.043	0.496MetHis: 0.496 ± 0.02	1.081MetIle: 1.081 ± 0.031	1.121MetLys: 1.121 ± 0.035	2.744MetLeu: 2.744 ± 0.044	0.61MetMet: 0.61 ± 0.024	0.82MetAsn: 0.82 ± 0.026	1.282MetPro: 1.282 ± 0.028	1.05MetGln: 1.05 ± 0.028	1.443MetArg: 1.443 ± 0.041	1.593MetSer: 1.593 ± 0.036	1.496MetThr: 1.496 ± 0.031	1.717MetVal: 1.717 ± 0.043	0.201MetTrp: 0.201 ± 0.015	0.364MetTyr: 0.364 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	2.993AsnAla: 2.993 ± 0.045	0.296AsnCys: 0.296 ± 0.016	1.414AsnAsp: 1.414 ± 0.037	1.356AsnGlu: 1.356 ± 0.036	0.901AsnPhe: 0.901 ± 0.028	2.363AsnGly: 2.363 ± 0.056	0.601AsnHis: 0.601 ± 0.021	1.313AsnIle: 1.313 ± 0.034	0.928AsnLys: 0.928 ± 0.028	3.084AsnLeu: 3.084 ± 0.045	0.603AsnMet: 0.603 ± 0.024	0.865AsnAsn: 0.865 ± 0.035	1.924AsnPro: 1.924 ± 0.038	1.052AsnGln: 1.052 ± 0.033	1.785AsnArg: 1.785 ± 0.039	1.311AsnSer: 1.311 ± 0.041	1.425AsnThr: 1.425 ± 0.043	2.006AsnVal: 2.006 ± 0.047	0.413AsnTrp: 0.413 ± 0.019	0.746AsnTyr: 0.746 ± 0.026	0.0AsnXaa: 0.0 ± 0.0
Pro	6.508ProAla: 6.508 ± 0.089	0.398ProCys: 0.398 ± 0.017	3.091ProAsp: 3.091 ± 0.055	3.491ProGlu: 3.491 ± 0.057	1.866ProPhe: 1.866 ± 0.037	4.138ProGly: 4.138 ± 0.061	1.195ProHis: 1.195 ± 0.034	1.891ProIle: 1.891 ± 0.045	1.485ProLys: 1.485 ± 0.041	5.789ProLeu: 5.789 ± 0.082	1.049ProMet: 1.049 ± 0.03	1.292ProAsn: 1.292 ± 0.033	2.398ProPro: 2.398 ± 0.058	1.954ProGln: 1.954 ± 0.04	2.696ProArg: 2.696 ± 0.046	2.67ProSer: 2.67 ± 0.051	2.183ProThr: 2.183 ± 0.049	4.074ProVal: 4.074 ± 0.057	0.719ProTrp: 0.719 ± 0.025	1.234ProTyr: 1.234 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	5.031GlnAla: 5.031 ± 0.077	0.305GlnCys: 0.305 ± 0.017	1.714GlnAsp: 1.714 ± 0.036	1.882GlnGlu: 1.882 ± 0.045	1.245GlnPhe: 1.245 ± 0.033	3.251GlnGly: 3.251 ± 0.05	1.117GlnHis: 1.117 ± 0.029	1.81GlnIle: 1.81 ± 0.037	1.21GlnLys: 1.21 ± 0.034	5.066GlnLeu: 5.066 ± 0.075	0.915GlnMet: 0.915 ± 0.028	0.959GlnAsn: 0.959 ± 0.03	2.383GlnPro: 2.383 ± 0.044	2.488GlnGln: 2.488 ± 0.066	3.286GlnArg: 3.286 ± 0.062	2.055GlnSer: 2.055 ± 0.042	1.817GlnThr: 1.817 ± 0.044	2.829GlnVal: 2.829 ± 0.051	0.602GlnTrp: 0.602 ± 0.021	0.925GlnTyr: 0.925 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	6.569ArgAla: 6.569 ± 0.089	0.638ArgCys: 0.638 ± 0.022	3.529ArgAsp: 3.529 ± 0.056	4.156ArgGlu: 4.156 ± 0.065	2.923ArgPhe: 2.923 ± 0.05	3.961ArgGly: 3.961 ± 0.053	2.18ArgHis: 2.18 ± 0.043	3.163ArgIle: 3.163 ± 0.054	2.02ArgLys: 2.02 ± 0.043	8.906ArgLeu: 8.906 ± 0.118	1.558ArgMet: 1.558 ± 0.036	1.677ArgAsn: 1.677 ± 0.038	3.082ArgPro: 3.082 ± 0.051	3.637ArgGln: 3.637 ± 0.073	5.065ArgArg: 5.065 ± 0.088	2.976ArgSer: 2.976 ± 0.048	2.591ArgThr: 2.591 ± 0.045	4.768ArgVal: 4.768 ± 0.074	1.057ArgTrp: 1.057 ± 0.033	2.133ArgTyr: 2.133 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	6.229SerAla: 6.229 ± 0.082	0.538SerCys: 0.538 ± 0.022	2.603SerAsp: 2.603 ± 0.044	2.799SerGlu: 2.799 ± 0.043	2.155SerPhe: 2.155 ± 0.044	5.349SerGly: 5.349 ± 0.081	1.284SerHis: 1.284 ± 0.031	2.492SerIle: 2.492 ± 0.052	1.654SerLys: 1.654 ± 0.037	6.048SerLeu: 6.048 ± 0.078	1.248SerMet: 1.248 ± 0.033	1.496SerAsn: 1.496 ± 0.037	2.745SerPro: 2.745 ± 0.056	2.061SerGln: 2.061 ± 0.044	3.709SerArg: 3.709 ± 0.058	3.17SerSer: 3.17 ± 0.072	2.755SerThr: 2.755 ± 0.057	3.851SerVal: 3.851 ± 0.059	0.763SerTrp: 0.763 ± 0.028	1.473SerTyr: 1.473 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	5.335ThrAla: 5.335 ± 0.077	0.43ThrCys: 0.43 ± 0.018	2.229ThrAsp: 2.229 ± 0.044	2.302ThrGlu: 2.302 ± 0.045	1.754ThrPhe: 1.754 ± 0.04	4.144ThrGly: 4.144 ± 0.064	1.157ThrHis: 1.157 ± 0.034	2.256ThrIle: 2.256 ± 0.06	1.155ThrLys: 1.155 ± 0.032	7.166ThrLeu: 7.166 ± 0.096	1.024ThrMet: 1.024 ± 0.028	1.121ThrAsn: 1.121 ± 0.036	3.361ThrPro: 3.361 ± 0.057	1.713ThrGln: 1.713 ± 0.039	3.216ThrArg: 3.216 ± 0.049	2.512ThrSer: 2.512 ± 0.053	2.465ThrThr: 2.465 ± 0.056	4.183ThrVal: 4.183 ± 0.065	0.557ThrTrp: 0.557 ± 0.021	1.008ThrTyr: 1.008 ± 0.033	0.0ThrXaa: 0.0 ± 0.0
Val	8.933ValAla: 8.933 ± 0.094	0.774ValCys: 0.774 ± 0.025	4.005ValAsp: 4.005 ± 0.064	4.46ValGlu: 4.46 ± 0.064	2.684ValPhe: 2.684 ± 0.048	5.36ValGly: 5.36 ± 0.077	1.436ValHis: 1.436 ± 0.034	3.608ValIle: 3.608 ± 0.057	2.734ValLys: 2.734 ± 0.055	8.341ValLeu: 8.341 ± 0.101	1.842ValMet: 1.842 ± 0.037	2.156ValAsn: 2.156 ± 0.047	3.612ValPro: 3.612 ± 0.06	2.355ValGln: 2.355 ± 0.043	4.679ValArg: 4.679 ± 0.063	4.487ValSer: 4.487 ± 0.058	3.94ValThr: 3.94 ± 0.055	5.981ValVal: 5.981 ± 0.089	0.942ValTrp: 0.942 ± 0.027	1.654ValTyr: 1.654 ± 0.04	0.0ValXaa: 0.0 ± 0.0
Trp	1.184TrpAla: 1.184 ± 0.034	0.163TrpCys: 0.163 ± 0.01	0.553TrpAsp: 0.553 ± 0.021	0.594TrpGlu: 0.594 ± 0.024	0.527TrpPhe: 0.527 ± 0.021	0.902TrpGly: 0.902 ± 0.028	0.473TrpHis: 0.473 ± 0.023	0.571TrpIle: 0.571 ± 0.024	0.46TrpLys: 0.46 ± 0.021	2.426TrpLeu: 2.426 ± 0.063	0.356TrpMet: 0.356 ± 0.016	0.419TrpAsn: 0.419 ± 0.017	0.711TrpPro: 0.711 ± 0.024	0.959TrpGln: 0.959 ± 0.033	1.182TrpArg: 1.182 ± 0.033	0.774TrpSer: 0.774 ± 0.025	0.517TrpThr: 0.517 ± 0.021	0.908TrpVal: 0.908 ± 0.026	0.246TrpTrp: 0.246 ± 0.017	0.307TrpTyr: 0.307 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.543TyrAla: 2.543 ± 0.049	0.297TyrCys: 0.297 ± 0.014	1.391TyrAsp: 1.391 ± 0.035	1.065TyrGlu: 1.065 ± 0.032	1.012TyrPhe: 1.012 ± 0.033	2.108TyrGly: 2.108 ± 0.045	0.613TyrHis: 0.613 ± 0.022	0.881TyrIle: 0.881 ± 0.026	0.707TyrLys: 0.707 ± 0.023	3.09TyrLeu: 3.09 ± 0.047	0.45TyrMet: 0.45 ± 0.022	0.715TyrAsn: 0.715 ± 0.028	1.399TyrPro: 1.399 ± 0.037	1.182TyrGln: 1.182 ± 0.034	2.093TyrArg: 2.093 ± 0.038	1.302TyrSer: 1.302 ± 0.03	1.245TyrThr: 1.245 ± 0.033	1.617TyrVal: 1.617 ± 0.041	0.42TyrTrp: 0.42 ± 0.019	0.758TyrTyr: 0.758 ± 0.027	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3958 proteins (1248374 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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