Nocardioides sp. Root151

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides; unclassified Nocardioides

Average proteome isoelectric point is 5.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4609 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q7YYE7|A0A0Q7YYE7_9ACTN Uncharacterized protein OS=Nocardioides sp. Root151 OX=1736475 GN=ASD66_17380 PE=4 SV=1
MM1 pKa = 7.62PGSATALSGGATVDD15 pKa = 3.65PQPAALADD23 pKa = 4.05APDD26 pKa = 3.91IPLSAVTAHH35 pKa = 6.83LEE37 pKa = 4.11EE38 pKa = 4.49FQSIAEE44 pKa = 4.12ANGGNRR50 pKa = 11.84YY51 pKa = 7.69TGQPGYY57 pKa = 9.87RR58 pKa = 11.84ASLDD62 pKa = 3.62YY63 pKa = 11.31VQAQLDD69 pKa = 3.58AAGFVTEE76 pKa = 4.24VQSFSTRR83 pKa = 11.84AGTSYY88 pKa = 11.52NLIADD93 pKa = 4.15WPGGDD98 pKa = 3.65PTNTVMMGGHH108 pKa = 7.3LDD110 pKa = 3.83SVDD113 pKa = 3.77EE114 pKa = 4.69GPGINDD120 pKa = 3.58NGTGSAAILASALAFAQSGAEE141 pKa = 4.05PTNHH145 pKa = 5.17VRR147 pKa = 11.84FGFWGAEE154 pKa = 3.85EE155 pKa = 4.8LGLLGSTHH163 pKa = 6.15YY164 pKa = 10.84VDD166 pKa = 4.09SLSTVEE172 pKa = 4.29VDD174 pKa = 5.21DD175 pKa = 4.1LAAYY179 pKa = 10.0FNFDD183 pKa = 3.26MVGSPNPGYY192 pKa = 10.09FVYY195 pKa = 10.76DD196 pKa = 3.54DD197 pKa = 4.6DD198 pKa = 6.65ANGTEE203 pKa = 4.79LRR205 pKa = 11.84DD206 pKa = 3.84SLTASYY212 pKa = 10.51DD213 pKa = 3.32AAGIDD218 pKa = 3.6SDD220 pKa = 4.85YY221 pKa = 11.09IDD223 pKa = 3.28VDD225 pKa = 3.49GRR227 pKa = 11.84SDD229 pKa = 3.03HH230 pKa = 6.61AAFMQRR236 pKa = 11.84GIPTAGTFSGAEE248 pKa = 4.06GTKK251 pKa = 10.12TSTQAAKK258 pKa = 9.91WGGQAGQAYY267 pKa = 9.24DD268 pKa = 3.6PCYY271 pKa = 10.54HH272 pKa = 6.43SACDD276 pKa = 5.79DD277 pKa = 3.48IDD279 pKa = 4.31NLDD282 pKa = 3.67TAALDD287 pKa = 4.05LNADD291 pKa = 5.01LIADD295 pKa = 4.41TLWAWADD302 pKa = 3.16HH303 pKa = 6.99DD304 pKa = 4.44FGGGTTEE311 pKa = 4.92PPTGDD316 pKa = 3.91DD317 pKa = 4.45AITNGGFEE325 pKa = 5.28DD326 pKa = 5.28GTTGWQATNGVVTTDD341 pKa = 4.23LDD343 pKa = 3.75QSAHH347 pKa = 5.91GGSGKK352 pKa = 10.44AWLNGYY358 pKa = 8.88GRR360 pKa = 11.84AHH362 pKa = 6.8TDD364 pKa = 2.84SVAQTVTVPDD374 pKa = 3.83AGRR377 pKa = 11.84LTFWLRR383 pKa = 11.84VDD385 pKa = 3.51TDD387 pKa = 3.37EE388 pKa = 4.83DD389 pKa = 4.32TTGQAYY395 pKa = 7.84DD396 pKa = 3.67TLRR399 pKa = 11.84VKK401 pKa = 10.79AGSTTLGTWSNLDD414 pKa = 3.44AGDD417 pKa = 3.85YY418 pKa = 10.74RR419 pKa = 11.84SIALDD424 pKa = 3.69LSAYY428 pKa = 9.9AGQSVALTFVGTEE441 pKa = 3.98DD442 pKa = 4.52AYY444 pKa = 11.18LASDD448 pKa = 3.8FVIDD452 pKa = 5.04DD453 pKa = 3.76VSLTSAGG460 pKa = 3.5

Molecular weight: 47.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q7Z7H2|A0A0Q7Z7H2_9ACTN Probable nicotinate-nucleotide adenylyltransferase OS=Nocardioides sp. Root151 OX=1736475 GN=nadD PE=3 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.93RR4 pKa = 11.84TYY6 pKa = 10.0QPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84HH14 pKa = 4.97KK15 pKa = 10.04VHH17 pKa = 6.59GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSTRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.87GRR40 pKa = 11.84SKK42 pKa = 10.84LAVV45 pKa = 3.25

Molecular weight: 5.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4609

0

4609

1475871

37

1974

320.2

34.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.45 ± 0.045

0.738 ± 0.009

6.726 ± 0.03

5.867 ± 0.034

2.974 ± 0.019

9.079 ± 0.034

2.281 ± 0.019

3.784 ± 0.024

2.333 ± 0.025

10.128 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.004 ± 0.014

2.03 ± 0.018

5.345 ± 0.023

2.767 ± 0.017

7.19 ± 0.04

5.482 ± 0.02

6.181 ± 0.032

9.18 ± 0.032

1.512 ± 0.017

1.946 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski