Amino acid dipepetide frequency for Nocardioides sp. Root151

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.207AlaAla: 17.207 ± 0.148	1.019AlaCys: 1.019 ± 0.028	8.411AlaAsp: 8.411 ± 0.082	7.85AlaGlu: 7.85 ± 0.076	3.619AlaPhe: 3.619 ± 0.057	11.46AlaGly: 11.46 ± 0.102	2.548AlaHis: 2.548 ± 0.044	4.602AlaIle: 4.602 ± 0.07	2.869AlaLys: 2.869 ± 0.054	12.763AlaLeu: 12.763 ± 0.118	2.875AlaMet: 2.875 ± 0.046	2.271AlaAsn: 2.271 ± 0.043	5.609AlaPro: 5.609 ± 0.087	3.38AlaGln: 3.38 ± 0.05	8.773AlaArg: 8.773 ± 0.095	6.602AlaSer: 6.602 ± 0.071	7.384AlaThr: 7.384 ± 0.081	10.849AlaVal: 10.849 ± 0.109	1.911AlaTrp: 1.911 ± 0.037	2.502AlaTyr: 2.502 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	0.926CysAla: 0.926 ± 0.026	0.094CysCys: 0.094 ± 0.008	0.488CysAsp: 0.488 ± 0.018	0.388CysGlu: 0.388 ± 0.016	0.23CysPhe: 0.23 ± 0.013	0.844CysGly: 0.844 ± 0.026	0.189CysHis: 0.189 ± 0.01	0.206CysIle: 0.206 ± 0.012	0.135CysLys: 0.135 ± 0.01	0.713CysLeu: 0.713 ± 0.022	0.117CysMet: 0.117 ± 0.009	0.148CysAsn: 0.148 ± 0.01	0.408CysPro: 0.408 ± 0.016	0.17CysGln: 0.17 ± 0.01	0.523CysArg: 0.523 ± 0.021	0.454CysSer: 0.454 ± 0.019	0.478CysThr: 0.478 ± 0.018	0.631CysVal: 0.631 ± 0.019	0.096CysTrp: 0.096 ± 0.007	0.144CysTyr: 0.144 ± 0.009	0.001CysXaa: 0.001 ± 0.001
Asp	7.829AspAla: 7.829 ± 0.079	0.381AspCys: 0.381 ± 0.016	5.257AspAsp: 5.257 ± 0.072	4.817AspGlu: 4.817 ± 0.063	1.871AspPhe: 1.871 ± 0.041	6.614AspGly: 6.614 ± 0.088	1.646AspHis: 1.646 ± 0.032	2.146AspIle: 2.146 ± 0.043	1.453AspLys: 1.453 ± 0.035	7.53AspLeu: 7.53 ± 0.078	0.911AspMet: 0.911 ± 0.026	1.182AspAsn: 1.182 ± 0.032	4.457AspPro: 4.457 ± 0.06	1.944AspGln: 1.944 ± 0.04	4.606AspArg: 4.606 ± 0.065	2.909AspSer: 2.909 ± 0.041	3.124AspThr: 3.124 ± 0.052	6.261AspVal: 6.261 ± 0.076	1.012AspTrp: 1.012 ± 0.026	1.312AspTyr: 1.312 ± 0.036	0.001AspXaa: 0.001 ± 0.001
Glu	7.187GluAla: 7.187 ± 0.078	0.36GluCys: 0.36 ± 0.015	2.991GluAsp: 2.991 ± 0.054	3.342GluGlu: 3.342 ± 0.054	1.775GluPhe: 1.775 ± 0.037	4.401GluGly: 4.401 ± 0.059	1.676GluHis: 1.676 ± 0.034	2.753GluIle: 2.753 ± 0.044	1.705GluLys: 1.705 ± 0.04	6.519GluLeu: 6.519 ± 0.08	1.166GluMet: 1.166 ± 0.028	1.157GluAsn: 1.157 ± 0.029	3.138GluPro: 3.138 ± 0.052	2.325GluGln: 2.325 ± 0.041	4.692GluArg: 4.692 ± 0.071	3.16GluSer: 3.16 ± 0.05	3.188GluThr: 3.188 ± 0.043	5.294GluVal: 5.294 ± 0.063	0.873GluTrp: 0.873 ± 0.023	0.97GluTyr: 0.97 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	3.714PheAla: 3.714 ± 0.054	0.282PheCys: 0.282 ± 0.013	2.279PheAsp: 2.279 ± 0.037	1.777PheGlu: 1.777 ± 0.038	0.934PhePhe: 0.934 ± 0.023	3.248PheGly: 3.248 ± 0.056	0.701PheHis: 0.701 ± 0.024	0.922PheIle: 0.922 ± 0.03	0.581PheLys: 0.581 ± 0.021	2.758PheLeu: 2.758 ± 0.049	0.505PheMet: 0.505 ± 0.018	0.695PheAsn: 0.695 ± 0.022	1.382PhePro: 1.382 ± 0.032	0.635PheGln: 0.635 ± 0.02	1.845PheArg: 1.845 ± 0.037	1.603PheSer: 1.603 ± 0.031	2.081PheThr: 2.081 ± 0.037	2.735PheVal: 2.735 ± 0.044	0.436PheTrp: 0.436 ± 0.019	0.631PheTyr: 0.631 ± 0.021	0.0PheXaa: 0.0 ± 0.0
Gly	9.892GlyAla: 9.892 ± 0.098	0.792GlyCys: 0.792 ± 0.026	5.818GlyAsp: 5.818 ± 0.067	5.053GlyGlu: 5.053 ± 0.057	3.141GlyPhe: 3.141 ± 0.052	7.671GlyGly: 7.671 ± 0.096	2.173GlyHis: 2.173 ± 0.041	3.777GlyIle: 3.777 ± 0.058	2.475GlyLys: 2.475 ± 0.046	9.394GlyLeu: 9.394 ± 0.101	2.046GlyMet: 2.046 ± 0.037	2.009GlyAsn: 2.009 ± 0.042	4.169GlyPro: 4.169 ± 0.061	2.737GlyGln: 2.737 ± 0.049	6.587GlyArg: 6.587 ± 0.072	5.425GlySer: 5.425 ± 0.068	5.911GlyThr: 5.911 ± 0.077	7.978GlyVal: 7.978 ± 0.096	1.65GlyTrp: 1.65 ± 0.035	2.098GlyTyr: 2.098 ± 0.039	0.0GlyXaa: 0.0 ± 0.0
His	2.599HisAla: 2.599 ± 0.042	0.196HisCys: 0.196 ± 0.011	1.686HisAsp: 1.686 ± 0.035	1.351HisGlu: 1.351 ± 0.033	0.663HisPhe: 0.663 ± 0.022	2.256HisGly: 2.256 ± 0.042	0.718HisHis: 0.718 ± 0.023	0.625HisIle: 0.625 ± 0.021	0.392HisLys: 0.392 ± 0.016	2.45HisLeu: 2.45 ± 0.047	0.364HisMet: 0.364 ± 0.017	0.414HisAsn: 0.414 ± 0.017	1.611HisPro: 1.611 ± 0.036	0.648HisGln: 0.648 ± 0.02	1.796HisArg: 1.796 ± 0.038	0.974HisSer: 0.974 ± 0.027	1.169HisThr: 1.169 ± 0.025	2.05HisVal: 2.05 ± 0.037	0.373HisTrp: 0.373 ± 0.017	0.474HisTyr: 0.474 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	5.305IleAla: 5.305 ± 0.067	0.312IleCys: 0.312 ± 0.015	3.077IleAsp: 3.077 ± 0.047	2.577IleGlu: 2.577 ± 0.047	0.948IlePhe: 0.948 ± 0.025	4.161IleGly: 4.161 ± 0.065	0.755IleHis: 0.755 ± 0.024	1.226IleIle: 1.226 ± 0.03	0.902IleLys: 0.902 ± 0.027	2.794IleLeu: 2.794 ± 0.05	0.55IleMet: 0.55 ± 0.02	0.974IleAsn: 0.974 ± 0.032	1.927IlePro: 1.927 ± 0.039	0.81IleGln: 0.81 ± 0.025	2.43IleArg: 2.43 ± 0.045	2.158IleSer: 2.158 ± 0.036	2.476IleThr: 2.476 ± 0.043	3.347IleVal: 3.347 ± 0.057	0.451IleTrp: 0.451 ± 0.019	0.664IleTyr: 0.664 ± 0.022	0.0IleXaa: 0.0 ± 0.0
Lys	3.086LysAla: 3.086 ± 0.062	0.117LysCys: 0.117 ± 0.009	1.448LysAsp: 1.448 ± 0.032	1.227LysGlu: 1.227 ± 0.033	0.635LysPhe: 0.635 ± 0.023	1.867LysGly: 1.867 ± 0.042	0.486LysHis: 0.486 ± 0.016	0.983LysIle: 0.983 ± 0.031	0.907LysLys: 0.907 ± 0.031	2.086LysLeu: 2.086 ± 0.041	0.457LysMet: 0.457 ± 0.017	0.545LysAsn: 0.545 ± 0.022	1.35LysPro: 1.35 ± 0.035	0.79LysGln: 0.79 ± 0.026	1.524LysArg: 1.524 ± 0.032	1.304LysSer: 1.304 ± 0.033	1.326LysThr: 1.326 ± 0.034	2.413LysVal: 2.413 ± 0.043	0.285LysTrp: 0.285 ± 0.014	0.497LysTyr: 0.497 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	14.276LeuAla: 14.276 ± 0.114	0.747LeuCys: 0.747 ± 0.021	7.215LeuAsp: 7.215 ± 0.081	5.641LeuGlu: 5.641 ± 0.072	2.582LeuPhe: 2.582 ± 0.047	9.4LeuGly: 9.4 ± 0.092	2.132LeuHis: 2.132 ± 0.041	3.389LeuIle: 3.389 ± 0.055	2.102LeuLys: 2.102 ± 0.041	10.233LeuLeu: 10.233 ± 0.126	1.857LeuMet: 1.857 ± 0.036	1.816LeuAsn: 1.816 ± 0.035	5.623LeuPro: 5.623 ± 0.066	2.286LeuGln: 2.286 ± 0.039	7.235LeuArg: 7.235 ± 0.079	5.484LeuSer: 5.484 ± 0.063	6.503LeuThr: 6.503 ± 0.06	10.128LeuVal: 10.128 ± 0.117	1.188LeuTrp: 1.188 ± 0.031	1.447LeuTyr: 1.447 ± 0.03	0.0LeuXaa: 0.0 ± 0.0
Met	2.355MetAla: 2.355 ± 0.037	0.151MetCys: 0.151 ± 0.011	0.972MetAsp: 0.972 ± 0.025	0.862MetGlu: 0.862 ± 0.026	0.56MetPhe: 0.56 ± 0.022	1.509MetGly: 1.509 ± 0.031	0.429MetHis: 0.429 ± 0.018	0.812MetIle: 0.812 ± 0.024	0.558MetLys: 0.558 ± 0.02	2.063MetLeu: 2.063 ± 0.036	0.409MetMet: 0.409 ± 0.02	0.499MetAsn: 0.499 ± 0.02	1.174MetPro: 1.174 ± 0.032	0.521MetGln: 0.521 ± 0.018	1.491MetArg: 1.491 ± 0.032	1.525MetSer: 1.525 ± 0.033	1.783MetThr: 1.783 ± 0.035	1.81MetVal: 1.81 ± 0.04	0.245MetTrp: 0.245 ± 0.014	0.308MetTyr: 0.308 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.436AsnAla: 2.436 ± 0.048	0.179AsnCys: 0.179 ± 0.011	1.266AsnAsp: 1.266 ± 0.032	1.039AsnGlu: 1.039 ± 0.026	0.579AsnPhe: 0.579 ± 0.023	2.032AsnGly: 2.032 ± 0.043	0.463AsnHis: 0.463 ± 0.017	0.762AsnIle: 0.762 ± 0.025	0.493AsnLys: 0.493 ± 0.019	2.08AsnLeu: 2.08 ± 0.04	0.367AsnMet: 0.367 ± 0.019	0.474AsnAsn: 0.474 ± 0.02	1.495AsnPro: 1.495 ± 0.036	0.629AsnGln: 0.629 ± 0.022	1.389AsnArg: 1.389 ± 0.03	0.955AsnSer: 0.955 ± 0.029	1.165AsnThr: 1.165 ± 0.027	1.697AsnVal: 1.697 ± 0.037	0.318AsnTrp: 0.318 ± 0.014	0.478AsnTyr: 0.478 ± 0.018	0.0AsnXaa: 0.0 ± 0.0
Pro	6.521ProAla: 6.521 ± 0.082	0.27ProCys: 0.27 ± 0.014	4.412ProAsp: 4.412 ± 0.065	3.956ProGlu: 3.956 ± 0.05	1.542ProPhe: 1.542 ± 0.034	5.328ProGly: 5.328 ± 0.068	1.154ProHis: 1.154 ± 0.031	1.812ProIle: 1.812 ± 0.033	1.197ProLys: 1.197 ± 0.029	4.588ProLeu: 4.588 ± 0.056	1.14ProMet: 1.14 ± 0.027	0.982ProAsn: 0.982 ± 0.025	2.43ProPro: 2.43 ± 0.058	1.419ProGln: 1.419 ± 0.035	3.277ProArg: 3.277 ± 0.054	3.014ProSer: 3.014 ± 0.046	3.476ProThr: 3.476 ± 0.048	4.935ProVal: 4.935 ± 0.066	0.883ProTrp: 0.883 ± 0.027	1.117ProTyr: 1.117 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	3.467GlnAla: 3.467 ± 0.046	0.185GlnCys: 0.185 ± 0.012	1.27GlnAsp: 1.27 ± 0.031	1.398GlnGlu: 1.398 ± 0.032	0.804GlnPhe: 0.804 ± 0.026	2.184GlnGly: 2.184 ± 0.036	0.678GlnHis: 0.678 ± 0.023	1.197GlnIle: 1.197 ± 0.032	0.644GlnLys: 0.644 ± 0.022	3.083GlnLeu: 3.083 ± 0.062	0.625GlnMet: 0.625 ± 0.021	0.48GlnAsn: 0.48 ± 0.018	1.617GlnPro: 1.617 ± 0.038	1.146GlnGln: 1.146 ± 0.035	2.39GlnArg: 2.39 ± 0.041	1.365GlnSer: 1.365 ± 0.029	1.426GlnThr: 1.426 ± 0.03	2.754GlnVal: 2.754 ± 0.042	0.452GlnTrp: 0.452 ± 0.018	0.5GlnTyr: 0.5 ± 0.021	0.001GlnXaa: 0.001 ± 0.001
Arg	8.202ArgAla: 8.202 ± 0.091	0.447ArgCys: 0.447 ± 0.017	4.446ArgAsp: 4.446 ± 0.061	4.273ArgGlu: 4.273 ± 0.064	2.281ArgPhe: 2.281 ± 0.039	5.225ArgGly: 5.225 ± 0.061	1.885ArgHis: 1.885 ± 0.04	3.27ArgIle: 3.27 ± 0.046	1.662ArgLys: 1.662 ± 0.043	7.606ArgLeu: 7.606 ± 0.076	1.758ArgMet: 1.758 ± 0.038	1.517ArgAsn: 1.517 ± 0.028	3.746ArgPro: 3.746 ± 0.049	2.103ArgGln: 2.103 ± 0.039	6.463ArgArg: 6.463 ± 0.091	4.005ArgSer: 4.005 ± 0.053	4.517ArgThr: 4.517 ± 0.061	5.889ArgVal: 5.889 ± 0.067	1.171ArgTrp: 1.171 ± 0.027	1.432ArgTyr: 1.432 ± 0.034	0.003ArgXaa: 0.003 ± 0.002
Ser	6.473SerAla: 6.473 ± 0.069	0.402SerCys: 0.402 ± 0.016	3.357SerAsp: 3.357 ± 0.046	2.738SerGlu: 2.738 ± 0.041	1.801SerPhe: 1.801 ± 0.036	5.835SerGly: 5.835 ± 0.06	1.069SerHis: 1.069 ± 0.028	2.084SerIle: 2.084 ± 0.032	1.199SerLys: 1.199 ± 0.028	5.339SerLeu: 5.339 ± 0.07	1.419SerMet: 1.419 ± 0.032	1.075SerAsn: 1.075 ± 0.027	3.008SerPro: 3.008 ± 0.046	1.401SerGln: 1.401 ± 0.029	3.781SerArg: 3.781 ± 0.057	3.355SerSer: 3.355 ± 0.058	3.628SerThr: 3.628 ± 0.055	4.641SerVal: 4.641 ± 0.064	0.917SerTrp: 0.917 ± 0.028	1.297SerTyr: 1.297 ± 0.032	0.0SerXaa: 0.0 ± 0.0
Thr	7.135ThrAla: 7.135 ± 0.077	0.473ThrCys: 0.473 ± 0.018	3.939ThrAsp: 3.939 ± 0.06	3.199ThrGlu: 3.199 ± 0.048	1.977ThrPhe: 1.977 ± 0.038	6.183ThrGly: 6.183 ± 0.07	1.307ThrHis: 1.307 ± 0.032	2.449ThrIle: 2.449 ± 0.046	1.351ThrLys: 1.351 ± 0.034	5.933ThrLeu: 5.933 ± 0.062	1.199ThrMet: 1.199 ± 0.028	1.288ThrAsn: 1.288 ± 0.033	3.798ThrPro: 3.798 ± 0.047	1.443ThrGln: 1.443 ± 0.033	3.982ThrArg: 3.982 ± 0.061	3.689ThrSer: 3.689 ± 0.052	4.214ThrThr: 4.214 ± 0.062	5.728ThrVal: 5.728 ± 0.061	1.069ThrTrp: 1.069 ± 0.028	1.447ThrTyr: 1.447 ± 0.034	0.001ThrXaa: 0.001 ± 0.001
Val	11.711ValAla: 11.711 ± 0.123	0.688ValCys: 0.688 ± 0.024	6.328ValAsp: 6.328 ± 0.07	5.496ValGlu: 5.496 ± 0.072	2.588ValPhe: 2.588 ± 0.045	7.544ValGly: 7.544 ± 0.085	2.072ValHis: 2.072 ± 0.04	3.716ValIle: 3.716 ± 0.06	1.953ValLys: 1.953 ± 0.043	9.573ValLeu: 9.573 ± 0.104	1.701ValMet: 1.701 ± 0.037	1.981ValAsn: 1.981 ± 0.04	4.849ValPro: 4.849 ± 0.064	2.18ValGln: 2.18 ± 0.038	6.46ValArg: 6.46 ± 0.069	4.836ValSer: 4.836 ± 0.058	5.963ValThr: 5.963 ± 0.073	9.621ValVal: 9.621 ± 0.105	1.149ValTrp: 1.149 ± 0.029	1.386ValTyr: 1.386 ± 0.032	0.001ValXaa: 0.001 ± 0.001
Trp	1.59TrpAla: 1.59 ± 0.037	0.144TrpCys: 0.144 ± 0.01	0.81TrpAsp: 0.81 ± 0.024	0.712TrpGlu: 0.712 ± 0.023	0.585TrpPhe: 0.585 ± 0.018	1.104TrpGly: 1.104 ± 0.029	0.394TrpHis: 0.394 ± 0.015	0.628TrpIle: 0.628 ± 0.02	0.352TrpLys: 0.352 ± 0.016	1.781TrpLeu: 1.781 ± 0.036	0.319TrpMet: 0.319 ± 0.016	0.398TrpAsn: 0.398 ± 0.017	0.72TrpPro: 0.72 ± 0.022	0.573TrpGln: 0.573 ± 0.02	1.18TrpArg: 1.18 ± 0.031	1.061TrpSer: 1.061 ± 0.029	0.959TrpThr: 0.959 ± 0.029	1.196TrpVal: 1.196 ± 0.029	0.32TrpTrp: 0.32 ± 0.015	0.297TrpTyr: 0.297 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.595TyrAla: 2.595 ± 0.044	0.144TyrCys: 0.144 ± 0.011	1.794TyrAsp: 1.794 ± 0.032	0.976TyrGlu: 0.976 ± 0.025	0.629TyrPhe: 0.629 ± 0.022	1.928TyrGly: 1.928 ± 0.042	0.333TyrHis: 0.333 ± 0.016	0.486TyrIle: 0.486 ± 0.019	0.4TyrLys: 0.4 ± 0.018	1.996TyrLeu: 1.996 ± 0.036	0.249TyrMet: 0.249 ± 0.014	0.39TyrAsn: 0.39 ± 0.016	0.942TyrPro: 0.942 ± 0.026	0.524TyrGln: 0.524 ± 0.018	1.477TyrArg: 1.477 ± 0.031	0.939TyrSer: 0.939 ± 0.029	1.035TyrThr: 1.035 ± 0.03	1.841TyrVal: 1.841 ± 0.035	0.325TyrTrp: 0.325 ± 0.015	0.453TyrTyr: 0.453 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.002XaaHis: 0.002 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.003XaaXaa: 0.003 ± 0.002

Statistics based on 4609 proteins (1475871 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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