Bovine papillomavirus type 3
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q8BDD9|VE8_BPV3 Uncharacterized protein E8 OS=Bovine papillomavirus type 3 OX=10561 PE=4 SV=1
MM1 pKa = 7.65 KK2 pKa = 10.5 GQDD5 pKa = 3.29 VTLKK9 pKa = 10.25 NVAVEE14 pKa = 4.32 LEE16 pKa = 4.55 DD17 pKa = 3.53 VVSPIILDD25 pKa = 3.6 CEE27 pKa = 4.23 EE28 pKa = 4.55 EE29 pKa = 4.3 IEE31 pKa = 4.34 TEE33 pKa = 4.32 EE34 pKa = 4.23 VDD36 pKa = 4.38 CPAPYY41 pKa = 10.19 AVEE44 pKa = 3.99 AVCYY48 pKa = 10.17 VCEE51 pKa = 4.06 NPLRR55 pKa = 11.84 LALVSSPDD63 pKa = 4.26 GIHH66 pKa = 6.42 QLHH69 pKa = 6.18 QLLLDD74 pKa = 5.13 CISLLCANCSRR85 pKa = 11.84 EE86 pKa = 4.24 VYY88 pKa = 10.38 SNRR91 pKa = 11.84 RR92 pKa = 11.84 PQRR95 pKa = 11.84 NGPP98 pKa = 3.53
Molecular weight: 10.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.165
IPC2_protein 4.406
IPC_protein 4.279
Toseland 4.126
ProMoST 4.38
Dawson 4.215
Bjellqvist 4.368
Wikipedia 4.088
Rodwell 4.113
Grimsley 4.037
Solomon 4.215
Lehninger 4.164
Nozaki 4.342
DTASelect 4.457
Thurlkill 4.139
EMBOSS 4.101
Sillero 4.393
Patrickios 0.693
IPC_peptide 4.215
IPC2_peptide 4.38
IPC2.peptide.svr19 4.292
Protein with the highest isoelectric point:
>sp|P50805|VL1_BPV3 Major capsid protein L1 OS=Bovine papillomavirus type 3 OX=10561 GN=L1 PE=3 SV=2
MM1 pKa = 7.75 KK2 pKa = 10.3 IFLQSSLPPGPNRR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 GPQTRR22 pKa = 11.84 GHH24 pKa = 6.77 PRR26 pKa = 11.84 HH27 pKa = 6.31 KK28 pKa = 9.91 SASFRR33 pKa = 11.84 RR34 pKa = 11.84 SASSGSDD41 pKa = 2.93 TRR43 pKa = 11.84 DD44 pKa = 2.83 SRR46 pKa = 11.84 GRR48 pKa = 11.84 SRR50 pKa = 11.84 SPSSSRR56 pKa = 11.84 SRR58 pKa = 11.84 SRR60 pKa = 11.84 SRR62 pKa = 11.84 SRR64 pKa = 11.84 SPSGSHH70 pKa = 5.28 SRR72 pKa = 11.84 PRR74 pKa = 11.84 APHH77 pKa = 6.3 VPDD80 pKa = 3.61 QEE82 pKa = 4.39 TGRR85 pKa = 11.84 PPGGGGRR92 pKa = 11.84 RR93 pKa = 11.84 GSRR96 pKa = 11.84 DD97 pKa = 3.34 QQQGPGGPAPPSPGEE112 pKa = 3.82 VGTRR116 pKa = 11.84 SGPPEE121 pKa = 3.93 TKK123 pKa = 10.48 AKK125 pKa = 10.29 GRR127 pKa = 11.84 LAEE130 pKa = 5.13 LISAAYY136 pKa = 9.2 DD137 pKa = 3.6 PPVLLLQGCANTLKK151 pKa = 10.42 SFRR154 pKa = 11.84 RR155 pKa = 11.84 RR156 pKa = 11.84 TTQSYY161 pKa = 8.82 PHH163 pKa = 6.91 TFLCMSTSWTWASKK177 pKa = 8.6 TCTVKK182 pKa = 10.42 SGHH185 pKa = 6.15 RR186 pKa = 11.84 MLVAFVNSEE195 pKa = 3.58 QRR197 pKa = 11.84 TLFLATVKK205 pKa = 10.11 IPKK208 pKa = 10.11 GVTCLKK214 pKa = 10.76 GSFDD218 pKa = 3.85 GLL220 pKa = 3.73
Molecular weight: 23.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.155
IPC2_protein 10.189
IPC_protein 11.652
Toseland 11.813
ProMoST 12.296
Dawson 11.813
Bjellqvist 11.798
Wikipedia 12.281
Rodwell 11.564
Grimsley 11.857
Solomon 12.296
Lehninger 12.193
Nozaki 11.813
DTASelect 11.798
Thurlkill 11.813
EMBOSS 12.31
Sillero 11.813
Patrickios 11.286
IPC_peptide 12.296
IPC2_peptide 11.286
IPC2.peptide.svr19 9.43
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
2682
75
613
298.0
33.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.078 ± 0.535
1.827 ± 0.468
5.928 ± 0.447
5.817 ± 0.694
4.325 ± 0.501
6.861 ± 0.844
2.461 ± 0.207
4.623 ± 0.641
4.512 ± 0.586
9.098 ± 1.135
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.752 ± 0.31
4.027 ± 0.785
7.159 ± 0.935
4.101 ± 0.555
7.047 ± 0.913
8.427 ± 0.9
6.376 ± 0.812
5.406 ± 0.797
1.454 ± 0.261
2.722 ± 0.36
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here