Lachancea dasiensis
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5089 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G4JBL8|A0A1G4JBL8_9SACH LADA_0E02894g1_1 OS=Lachancea dasiensis OX=1072105 GN=LADA_0E02894G PE=3 SV=1
MM1 pKa = 7.69 RR2 pKa = 11.84 AGLFFSALMAVATASPVALNDD23 pKa = 3.39 FVKK26 pKa = 10.43 RR27 pKa = 11.84 DD28 pKa = 3.72 SEE30 pKa = 4.31 DD31 pKa = 3.45 FDD33 pKa = 6.18 PIEE36 pKa = 4.26 AWPGNWTLSNWSNWTNSSNVTQWEE60 pKa = 4.54 SPKK63 pKa = 10.55 LQVFITGGHH72 pKa = 5.67 YY73 pKa = 10.53 KK74 pKa = 10.51 LSNWTSSPDD83 pKa = 3.38 VTITSLFNQTSLNATEE99 pKa = 5.02 LYY101 pKa = 10.54 SVASSVSKK109 pKa = 10.91 SLASDD114 pKa = 4.15 AYY116 pKa = 8.93 TGVVVLGHH124 pKa = 6.12 EE125 pKa = 4.4 KK126 pKa = 10.61 ALEE129 pKa = 3.95 SLGFFLSVVVDD140 pKa = 3.95 SPKK143 pKa = 10.99 SLVVTSKK150 pKa = 11.06 LDD152 pKa = 4.09 DD153 pKa = 4.33 GLTVAWSDD161 pKa = 3.64 DD162 pKa = 3.53 SWWRR166 pKa = 11.84 GTLIVDD172 pKa = 3.17 QTLIYY177 pKa = 10.24 SGAVYY182 pKa = 9.57 TPSTPGAVSIGAVYY196 pKa = 10.73 DD197 pKa = 3.4 KK198 pKa = 11.1 TPWFWFTPAWPMLLSPEE215 pKa = 4.08 STIRR219 pKa = 11.84 TEE221 pKa = 3.86 YY222 pKa = 10.63 WNFTDD227 pKa = 5.56 VIQANSTISTNGTTIPIVYY246 pKa = 9.51 DD247 pKa = 3.77 AYY249 pKa = 11.28 YY250 pKa = 11.01 NPGLLEE256 pKa = 4.03 YY257 pKa = 10.58 LSGSIDD263 pKa = 3.41 GLVVVSSGNSTGSEE277 pKa = 4.24 FTSASIPIVFASDD290 pKa = 3.15 DD291 pKa = 4.11 YY292 pKa = 11.85 YY293 pKa = 10.65 PVFVDD298 pKa = 5.12 DD299 pKa = 4.63 VPAGAIAAGGLSPVQSQLLLAIALTNNVTASSQLQDD335 pKa = 2.85 IFFSS339 pKa = 3.76
Molecular weight: 36.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.766
IPC2_protein 3.948
IPC_protein 3.948
Toseland 3.732
ProMoST 4.113
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.884
Rodwell 3.77
Grimsley 3.643
Solomon 3.935
Lehninger 3.897
Nozaki 4.062
DTASelect 4.317
Thurlkill 3.783
EMBOSS 3.897
Sillero 4.075
Patrickios 1.939
IPC_peptide 3.935
IPC2_peptide 4.05
IPC2.peptide.svr19 3.957
Protein with the highest isoelectric point:
>tr|A0A1G4JN28|A0A1G4JN28_9SACH LADA_0F14092g1_1 OS=Lachancea dasiensis OX=1072105 GN=LADA_0F14092G PE=3 SV=1
MM1 pKa = 7.1 RR2 pKa = 11.84 AKK4 pKa = 9.09 WRR6 pKa = 11.84 KK7 pKa = 8.87 KK8 pKa = 8.73 RR9 pKa = 11.84 VRR11 pKa = 11.84 RR12 pKa = 11.84 LKK14 pKa = 9.71 RR15 pKa = 11.84 KK16 pKa = 8.27 RR17 pKa = 11.84 RR18 pKa = 11.84 KK19 pKa = 9.19 VRR21 pKa = 11.84 ARR23 pKa = 11.84 SKK25 pKa = 10.98
Molecular weight: 3.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5089
0
5089
2476411
25
4926
486.6
54.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.735 ± 0.029
1.323 ± 0.012
5.76 ± 0.025
6.529 ± 0.033
4.353 ± 0.023
5.687 ± 0.032
2.313 ± 0.015
5.454 ± 0.023
6.292 ± 0.029
9.932 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.067 ± 0.011
4.732 ± 0.018
4.521 ± 0.025
4.279 ± 0.024
5.074 ± 0.022
8.988 ± 0.042
5.519 ± 0.02
6.256 ± 0.023
1.103 ± 0.012
3.083 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here