Leishmania RNA virus 2 - 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; Leishmaniavirus; Leishmania RNA virus 2

Average proteome isoelectric point is 7.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q83073|Q83073_9VIRU RNA-directed RNA polymerase OS=Leishmania RNA virus 2 - 1 OX=39116 PE=3 SV=1
MM1 pKa = 7.58TSPIPQMNTSVFNLTRR17 pKa = 11.84GRR19 pKa = 11.84RR20 pKa = 11.84VVSGDD25 pKa = 2.88TSEE28 pKa = 4.43RR29 pKa = 11.84VYY31 pKa = 10.55TAVLNYY37 pKa = 9.94CSTVRR42 pKa = 11.84QSYY45 pKa = 8.96QATTNVHH52 pKa = 4.73WRR54 pKa = 11.84LKK56 pKa = 10.14QRR58 pKa = 11.84GKK60 pKa = 10.34KK61 pKa = 9.54ASHH64 pKa = 6.32VSRR67 pKa = 11.84AHH69 pKa = 6.09AGISASFDD77 pKa = 3.38YY78 pKa = 11.21ADD80 pKa = 3.95NLKK83 pKa = 10.31QEE85 pKa = 3.89ILKK88 pKa = 8.93YY89 pKa = 10.22HH90 pKa = 5.97SRR92 pKa = 11.84HH93 pKa = 4.75VCTEE97 pKa = 3.67IVVGRR102 pKa = 11.84DD103 pKa = 3.05YY104 pKa = 11.42RR105 pKa = 11.84FDD107 pKa = 3.64FRR109 pKa = 11.84SLLHH113 pKa = 6.57HH114 pKa = 7.17AGACMAHH121 pKa = 5.9YY122 pKa = 10.34CLTGVLDD129 pKa = 4.64LNVLSSGKK137 pKa = 10.37SGGTRR142 pKa = 11.84VTVLSNADD150 pKa = 3.59GLSILATDD158 pKa = 4.1VLYY161 pKa = 11.01LPNALKK167 pKa = 10.69MMDD170 pKa = 3.52INIFNCIYY178 pKa = 10.57LLAAACEE185 pKa = 4.28CNIVFDD191 pKa = 5.26DD192 pKa = 4.66VILTGGTSAYY202 pKa = 10.04NVPALNSVTVEE213 pKa = 4.05YY214 pKa = 10.89APTLHH219 pKa = 6.71NYY221 pKa = 9.61ISVAFTSNGDD231 pKa = 3.76GGCDD235 pKa = 3.08GALALTAGLHH245 pKa = 4.72TVVTVVAHH253 pKa = 6.2SDD255 pKa = 2.97EE256 pKa = 4.69GGQMRR261 pKa = 11.84DD262 pKa = 3.15VLRR265 pKa = 11.84ALHH268 pKa = 5.69YY269 pKa = 10.66APAKK273 pKa = 9.81GIVVADD279 pKa = 3.94PNRR282 pKa = 11.84TVNHH286 pKa = 6.33SLPLLMYY293 pKa = 9.57EE294 pKa = 4.25PTWDD298 pKa = 4.13SVCGMWDD305 pKa = 3.46YY306 pKa = 11.61VAVATAGLVHH316 pKa = 6.94LCDD319 pKa = 3.83PCEE322 pKa = 4.49LIAHH326 pKa = 6.8DD327 pKa = 4.87VYY329 pKa = 10.36PTIITAPDD337 pKa = 3.56EE338 pKa = 4.45NNVRR342 pKa = 11.84AIEE345 pKa = 4.05ARR347 pKa = 11.84VEE349 pKa = 3.79AALPAFASIYY359 pKa = 9.47IHH361 pKa = 6.27NLAMFMGVGGDD372 pKa = 3.51DD373 pKa = 3.83CGRR376 pKa = 11.84AVDD379 pKa = 3.96TLVEE383 pKa = 4.27AGVYY387 pKa = 9.73IYY389 pKa = 11.2SLTGNDD395 pKa = 4.04ASRR398 pKa = 11.84HH399 pKa = 4.06MLKK402 pKa = 9.42GTMAPWFWVEE412 pKa = 3.84STGLFRR418 pKa = 11.84DD419 pKa = 4.37LTCFNIPGLSAGYY432 pKa = 9.78GPQAIYY438 pKa = 8.86GTAITQPAMQACEE451 pKa = 3.99YY452 pKa = 9.3TGTRR456 pKa = 11.84GSYY459 pKa = 10.39DD460 pKa = 3.7YY461 pKa = 11.7YY462 pKa = 10.96NVGWTSLRR470 pKa = 11.84KK471 pKa = 9.62HH472 pKa = 6.21PLLVLTNNRR481 pKa = 11.84AGDD484 pKa = 4.71GIAHH488 pKa = 6.04MEE490 pKa = 4.15VGRR493 pKa = 11.84DD494 pKa = 3.39AAIPWVLLGQPQRR507 pKa = 11.84RR508 pKa = 11.84EE509 pKa = 4.08CTAQGHH515 pKa = 5.22GTQTATCGHH524 pKa = 6.17TKK526 pKa = 10.21HH527 pKa = 6.51NRR529 pKa = 11.84GTLDD533 pKa = 3.02EE534 pKa = 4.84YY535 pKa = 10.28IWGRR539 pKa = 11.84FSTGLFHH546 pKa = 7.4PAEE549 pKa = 4.45LTTFTNVEE557 pKa = 4.15FRR559 pKa = 11.84VKK561 pKa = 10.24CWTEE565 pKa = 4.22DD566 pKa = 3.06NDD568 pKa = 4.49GNVLEE573 pKa = 4.91TGAPVKK579 pKa = 10.61DD580 pKa = 3.34IVEE583 pKa = 4.43GGVTVSVNAILLTNSTNHH601 pKa = 5.81IRR603 pKa = 11.84TVPAVRR609 pKa = 11.84RR610 pKa = 11.84SYY612 pKa = 10.7QAGAKK617 pKa = 9.14YY618 pKa = 10.54LEE620 pKa = 4.43EE621 pKa = 4.2ARR623 pKa = 11.84SRR625 pKa = 11.84GTMSTLNRR633 pKa = 11.84IIGGQLFRR641 pKa = 11.84DD642 pKa = 4.16LQPISKK648 pKa = 9.88RR649 pKa = 11.84GVPPQPEE656 pKa = 4.32PVCASMPVEE665 pKa = 4.19TTKK668 pKa = 10.68EE669 pKa = 3.97VSLSRR674 pKa = 11.84IGPIRR679 pKa = 11.84INQNVFRR686 pKa = 11.84PRR688 pKa = 11.84PQPTEE693 pKa = 3.97EE694 pKa = 4.23EE695 pKa = 4.19MHH697 pKa = 6.46EE698 pKa = 4.39EE699 pKa = 4.01PTPVALEE706 pKa = 4.05AEE708 pKa = 4.42PPGEE712 pKa = 4.11NVV714 pKa = 2.95

Molecular weight: 77.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q83072|Q83072_9VIRU Major capsid protein OS=Leishmania RNA virus 2 - 1 OX=39116 PE=4 SV=1
MM1 pKa = 7.02VCRR4 pKa = 11.84SVRR7 pKa = 11.84PAILILTPVDD17 pKa = 3.21AGKK20 pKa = 10.1NRR22 pKa = 11.84VMGWHH27 pKa = 6.48PAPYY31 pKa = 9.58VFQSLVYY38 pKa = 9.56YY39 pKa = 10.78

Molecular weight: 4.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1530

39

777

510.0

56.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.954 ± 0.199

2.484 ± 0.029

5.033 ± 0.414

5.359 ± 0.857

2.549 ± 0.019

6.797 ± 0.994

3.791 ± 0.275

4.575 ± 0.155

4.052 ± 1.259

8.954 ± 0.516

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.745 ± 0.402

4.248 ± 0.566

4.314 ± 1.081

2.418 ± 0.167

6.405 ± 0.382

6.34 ± 0.454

6.144 ± 1.471

8.954 ± 0.921

1.438 ± 0.192

4.444 ± 0.558
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski