CRESS virus sp.
Average proteome isoelectric point is 7.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A385E6C8|A0A385E6C8_9VIRU Putative capsid protein OS=CRESS virus sp. OX=2202563 PE=4 SV=1
MM1 pKa = 8.09 KK2 pKa = 9.82 IRR4 pKa = 11.84 CMCGTLNNYY13 pKa = 7.45 TVAGLAKK20 pKa = 10.65 VKK22 pKa = 10.76 DD23 pKa = 4.09 FLEE26 pKa = 4.09 NHH28 pKa = 5.77 CTYY31 pKa = 10.74 GVYY34 pKa = 10.57 GEE36 pKa = 4.59 EE37 pKa = 4.39 VGEE40 pKa = 4.32 SGTPHH45 pKa = 5.7 LQIYY49 pKa = 10.18 FEE51 pKa = 5.07 MKK53 pKa = 9.03 TQMTYY58 pKa = 10.73 SAVNKK63 pKa = 10.39 KK64 pKa = 10.56 LGFKK68 pKa = 10.47 KK69 pKa = 10.27 GGEE72 pKa = 4.21 WIDD75 pKa = 3.21 LSTRR79 pKa = 11.84 HH80 pKa = 5.47 GTPEE84 pKa = 3.56 QAAGYY89 pKa = 8.2 CKK91 pKa = 10.26 KK92 pKa = 10.79 GNEE95 pKa = 4.21 TEE97 pKa = 4.19 EE98 pKa = 4.49 VYY100 pKa = 10.81 PDD102 pKa = 3.12 GWTDD106 pKa = 3.41 GAAWWFTRR114 pKa = 11.84 PAASWIGDD122 pKa = 3.3 EE123 pKa = 4.66 WGTISNQGEE132 pKa = 4.18 RR133 pKa = 11.84 KK134 pKa = 9.55 DD135 pKa = 3.7 LKK137 pKa = 10.85 RR138 pKa = 11.84 KK139 pKa = 8.65 IDD141 pKa = 3.71 EE142 pKa = 4.69 VISGDD147 pKa = 3.6 TTPDD151 pKa = 3.83 EE152 pKa = 4.81 ICVEE156 pKa = 4.13 MPVFYY161 pKa = 10.26 HH162 pKa = 5.88 QYY164 pKa = 10.74 GRR166 pKa = 11.84 TLEE169 pKa = 4.23 KK170 pKa = 10.06 AWCIAMRR177 pKa = 11.84 KK178 pKa = 7.9 KK179 pKa = 9.63 WRR181 pKa = 11.84 TEE183 pKa = 3.3 MTTCDD188 pKa = 3.89 WLWGEE193 pKa = 4.18 TGVGKK198 pKa = 8.25 SHH200 pKa = 7.21 KK201 pKa = 10.15 AFEE204 pKa = 5.02 GYY206 pKa = 10.01 SPEE209 pKa = 3.83 THH211 pKa = 6.13 YY212 pKa = 11.24 VWNLAEE218 pKa = 4.59 EE219 pKa = 4.54 FQCGYY224 pKa = 9.79 RR225 pKa = 11.84 QQDD228 pKa = 3.38 TVIINEE234 pKa = 3.58 FRR236 pKa = 11.84 GQIKK240 pKa = 10.47 YY241 pKa = 10.61 SEE243 pKa = 4.82 LLTLIDD249 pKa = 4.49 KK250 pKa = 9.54 WPHH253 pKa = 5.12 CIKK256 pKa = 10.72 RR257 pKa = 11.84 KK258 pKa = 10.16 GGEE261 pKa = 3.74 QLPFVSKK268 pKa = 10.52 HH269 pKa = 6.23 IIITSCKK276 pKa = 9.26 PPDD279 pKa = 4.33 EE280 pKa = 4.53 IYY282 pKa = 10.4 PRR284 pKa = 11.84 QDD286 pKa = 2.9 EE287 pKa = 4.37 KK288 pKa = 11.71 DD289 pKa = 3.39 SLRR292 pKa = 11.84 QLLRR296 pKa = 11.84 RR297 pKa = 11.84 IKK299 pKa = 10.35 VIEE302 pKa = 4.19 VEE304 pKa = 4.15 KK305 pKa = 10.78 QPPVWGRR312 pKa = 11.84 APPYY316 pKa = 8.38 PPPMTACGGCGG327 pKa = 2.94
Molecular weight: 37.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.156
IPC2_protein 6.262
IPC_protein 6.338
Toseland 6.389
ProMoST 6.766
Dawson 6.737
Bjellqvist 6.722
Wikipedia 6.722
Rodwell 6.737
Grimsley 6.465
Solomon 6.751
Lehninger 6.751
Nozaki 7.029
DTASelect 7.117
Thurlkill 7.146
EMBOSS 7.132
Sillero 7.146
Patrickios 3.986
IPC_peptide 6.766
IPC2_peptide 6.839
IPC2.peptide.svr19 6.777
Protein with the highest isoelectric point:
>tr|A0A385E6C8|A0A385E6C8_9VIRU Putative capsid protein OS=CRESS virus sp. OX=2202563 PE=4 SV=1
MM1 pKa = 7.35 LRR3 pKa = 11.84 KK4 pKa = 9.93 KK5 pKa = 10.44 ISPKK9 pKa = 9.32 FKK11 pKa = 9.55 KK12 pKa = 9.77 RR13 pKa = 11.84 NMARR17 pKa = 11.84 RR18 pKa = 11.84 HH19 pKa = 5.02 YY20 pKa = 10.3 GKK22 pKa = 10.14 KK23 pKa = 9.74 RR24 pKa = 11.84 PRR26 pKa = 11.84 KK27 pKa = 5.41 TTSKK31 pKa = 10.48 AAIMYY36 pKa = 7.42 KK37 pKa = 10.14 KK38 pKa = 10.0 PSASNQQKK46 pKa = 10.16 QIAALAVKK54 pKa = 9.84 VNRR57 pKa = 11.84 NQRR60 pKa = 11.84 RR61 pKa = 11.84 INQQRR66 pKa = 11.84 YY67 pKa = 8.18 LVQHH71 pKa = 3.99 QTKK74 pKa = 9.95 FEE76 pKa = 4.12 EE77 pKa = 4.49 QPVTRR82 pKa = 11.84 PAGYY86 pKa = 10.46 APYY89 pKa = 9.14 TAWSLNNIPFMEE101 pKa = 4.53 QIFGDD106 pKa = 3.8 PEE108 pKa = 3.7 EE109 pKa = 4.66 AKK111 pKa = 10.53 GGKK114 pKa = 7.94 YY115 pKa = 8.49 TGKK118 pKa = 10.64 ALRR121 pKa = 11.84 LDD123 pKa = 3.76 FNIGIGKK130 pKa = 7.46 STRR133 pKa = 11.84 TTNFTAFIIRR143 pKa = 11.84 PKK145 pKa = 6.97 TQKK148 pKa = 9.93 VVNEE152 pKa = 4.49 CGISQNNVLSPTSVLPSPMIAGTDD176 pKa = 3.72 FSYY179 pKa = 11.09 EE180 pKa = 3.83 GGLALINPKK189 pKa = 9.53 RR190 pKa = 11.84 WHH192 pKa = 6.5 IDD194 pKa = 2.6 KK195 pKa = 10.45 MMKK198 pKa = 10.1 LQIRR202 pKa = 11.84 PQVAPFYY209 pKa = 10.4 QGTPPTPTNVVTQDD223 pKa = 2.65 NRR225 pKa = 11.84 VRR227 pKa = 11.84 RR228 pKa = 11.84 SCTLKK233 pKa = 10.36 NPMYY237 pKa = 10.23 INSRR241 pKa = 11.84 TGDD244 pKa = 3.33 WKK246 pKa = 8.64 TTTDD250 pKa = 3.23 PWEE253 pKa = 4.18 LPARR257 pKa = 11.84 QRR259 pKa = 11.84 SFLVLFDD266 pKa = 4.51 DD267 pKa = 4.8 TPATTGTSPGPTFEE281 pKa = 4.62 GLVHH285 pKa = 6.06 LTAHH289 pKa = 6.01 TSEE292 pKa = 4.32
Molecular weight: 33.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.28
IPC2_protein 9.765
IPC_protein 10.145
Toseland 10.672
ProMoST 10.248
Dawson 10.774
Bjellqvist 10.409
Wikipedia 10.921
Rodwell 11.242
Grimsley 10.818
Solomon 10.833
Lehninger 10.804
Nozaki 10.643
DTASelect 10.409
Thurlkill 10.672
EMBOSS 11.067
Sillero 10.687
Patrickios 10.935
IPC_peptide 10.833
IPC2_peptide 9.077
IPC2.peptide.svr19 8.675
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
619
292
327
309.5
35.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.493 ± 0.658
2.262 ± 1.023
3.877 ± 0.516
6.3 ± 1.866
3.393 ± 0.465
7.754 ± 1.254
2.1 ± 0.252
5.816 ± 0.218
8.401 ± 0.104
5.493 ± 0.214
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.585 ± 0.101
4.039 ± 1.157
6.947 ± 0.826
4.847 ± 0.633
6.139 ± 0.905
4.523 ± 0.62
8.562 ± 0.889
4.847 ± 0.034
2.746 ± 0.893
3.877 ± 0.516
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here