Capybara microvirus Cap1_SP_87
Average proteome isoelectric point is 5.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W529|A0A4P8W529_9VIRU Major capsid protein OS=Capybara microvirus Cap1_SP_87 OX=2584796 PE=3 SV=1
MM1 pKa = 7.54 KK2 pKa = 9.58 VTKK5 pKa = 10.23 LLYY8 pKa = 10.56 LEE10 pKa = 4.3 NPRR13 pKa = 11.84 YY14 pKa = 9.56 ASPIIPDD21 pKa = 3.12 QVMEE25 pKa = 4.46 KK26 pKa = 10.44 EE27 pKa = 4.1 YY28 pKa = 10.93 TEE30 pKa = 4.88 DD31 pKa = 3.81 GNVVQVWKK39 pKa = 10.83 VKK41 pKa = 10.81 SNYY44 pKa = 9.28 EE45 pKa = 4.05 DD46 pKa = 5.33 LIEE49 pKa = 4.57 LHH51 pKa = 7.01 GDD53 pKa = 3.33 ANNWSIDD60 pKa = 3.43 ALMKK64 pKa = 10.8 AGIDD68 pKa = 3.62 PASTSINTGKK78 pKa = 10.27 NSRR81 pKa = 11.84 LDD83 pKa = 3.67 GYY85 pKa = 11.49 NDD87 pKa = 3.46 LGKK90 pKa = 10.62 FIDD93 pKa = 4.3 GFEE96 pKa = 4.1 VLTDD100 pKa = 3.93 EE101 pKa = 6.04 GDD103 pKa = 3.25 IDD105 pKa = 4.26 NNN107 pKa = 3.74
Molecular weight: 12.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.375
IPC2_protein 4.253
IPC_protein 4.177
Toseland 3.986
ProMoST 4.329
Dawson 4.164
Bjellqvist 4.317
Wikipedia 4.075
Rodwell 4.012
Grimsley 3.897
Solomon 4.151
Lehninger 4.101
Nozaki 4.279
DTASelect 4.495
Thurlkill 4.024
EMBOSS 4.088
Sillero 4.304
Patrickios 3.808
IPC_peptide 4.151
IPC2_peptide 4.279
IPC2.peptide.svr19 4.232
Protein with the highest isoelectric point:
>tr|A0A4P8W7M9|A0A4P8W7M9_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_87 OX=2584796 PE=4 SV=1
MM1 pKa = 7.51 FKK3 pKa = 10.0 TLTVKK8 pKa = 9.25 NTTHH12 pKa = 6.29 YY13 pKa = 11.32 VDD15 pKa = 4.36 LSRR18 pKa = 11.84 SKK20 pKa = 10.19 ICNSFRR26 pKa = 11.84 NPSAFQSEE34 pKa = 4.32 EE35 pKa = 4.19 LKK37 pKa = 10.34 RR38 pKa = 11.84 QSYY41 pKa = 7.25 EE42 pKa = 3.28 ARR44 pKa = 11.84 LYY46 pKa = 10.96 YY47 pKa = 10.25 EE48 pKa = 4.72 YY49 pKa = 11.0 LDD51 pKa = 3.85 CQRR54 pKa = 11.84 RR55 pKa = 11.84 KK56 pKa = 10.54 CPVFFYY62 pKa = 10.7 TLTFNNMHH70 pKa = 6.64 LPHH73 pKa = 7.3 YY74 pKa = 8.51 EE75 pKa = 3.75 GHH77 pKa = 5.8 VCFDD81 pKa = 4.08 NKK83 pKa = 10.57 ILMQFMNSLRR93 pKa = 11.84 KK94 pKa = 9.62 RR95 pKa = 11.84 LVRR98 pKa = 11.84 DD99 pKa = 3.17 YY100 pKa = 11.41 GCNFKK105 pKa = 11.2 YY106 pKa = 10.17 FVSCEE111 pKa = 3.94 MGEE114 pKa = 4.33 GAGTRR119 pKa = 11.84 KK120 pKa = 9.85 EE121 pKa = 4.22 YY122 pKa = 10.78 EE123 pKa = 3.87 NPHH126 pKa = 4.88 YY127 pKa = 10.44 HH128 pKa = 6.38 VLFFTYY134 pKa = 10.43 AEE136 pKa = 4.36 PDD138 pKa = 3.33 HH139 pKa = 6.72 YY140 pKa = 10.46 FVRR143 pKa = 11.84 PTVYY147 pKa = 10.4 DD148 pKa = 3.98 FKK150 pKa = 11.48 KK151 pKa = 10.35 LVRR154 pKa = 11.84 LYY156 pKa = 10.62 WMGQEE161 pKa = 3.64 KK162 pKa = 10.43 VGYY165 pKa = 9.73 INYY168 pKa = 7.73 KK169 pKa = 5.18 TAKK172 pKa = 9.64 YY173 pKa = 8.76 GISMEE178 pKa = 4.23 GDD180 pKa = 2.98 HH181 pKa = 7.07 CGLVLNFNACTYY193 pKa = 8.1 VAKK196 pKa = 10.28 YY197 pKa = 9.37 VCKK200 pKa = 10.14 DD201 pKa = 3.05 TTKK204 pKa = 9.96 WKK206 pKa = 10.59 NIEE209 pKa = 4.16 STNLSDD215 pKa = 4.41 RR216 pKa = 11.84 LYY218 pKa = 11.34 SLVDD222 pKa = 3.45 DD223 pKa = 4.5 EE224 pKa = 5.25 QNSKK228 pKa = 11.13 EE229 pKa = 3.93 NMVEE233 pKa = 4.09 FFKK236 pKa = 11.45 SEE238 pKa = 4.12 FNPLLEE244 pKa = 4.68 GKK246 pKa = 9.75 SPQEE250 pKa = 3.74 WYY252 pKa = 10.39 DD253 pKa = 3.59 EE254 pKa = 4.4 YY255 pKa = 11.12 IEE257 pKa = 4.56 FLDD260 pKa = 4.9 TIPFTYY266 pKa = 10.52 DD267 pKa = 3.48 DD268 pKa = 5.33 LDD270 pKa = 5.4 SEE272 pKa = 5.25 DD273 pKa = 5.75 DD274 pKa = 4.13 YY275 pKa = 12.1 DD276 pKa = 5.31 DD277 pKa = 4.03 EE278 pKa = 4.59 EE279 pKa = 4.75 NYY281 pKa = 10.23 RR282 pKa = 11.84 EE283 pKa = 4.08 YY284 pKa = 11.29 LDD286 pKa = 4.4 KK287 pKa = 10.79 KK288 pKa = 9.53 AKK290 pKa = 9.84 KK291 pKa = 9.81 RR292 pKa = 11.84 VILDD296 pKa = 3.52 HH297 pKa = 5.82 IKK299 pKa = 10.34 KK300 pKa = 10.42 YY301 pKa = 10.39 KK302 pKa = 10.21 RR303 pKa = 11.84 CDD305 pKa = 4.26 DD306 pKa = 3.85 ILSHH310 pKa = 7.29 LDD312 pKa = 3.51 VIEE315 pKa = 4.61 TILYY319 pKa = 9.94 DD320 pKa = 3.72 FNLYY324 pKa = 10.76 DD325 pKa = 3.36 NEE327 pKa = 4.47 YY328 pKa = 11.21 YY329 pKa = 10.87 SFCCYY334 pKa = 10.61 LSDD337 pKa = 5.54 DD338 pKa = 4.4 KK339 pKa = 11.81 VSEE342 pKa = 4.04 QKK344 pKa = 10.58 KK345 pKa = 8.14 EE346 pKa = 3.79 FRR348 pKa = 11.84 SRR350 pKa = 11.84 YY351 pKa = 7.62 CPKK354 pKa = 10.54 VMISQGVGKK363 pKa = 10.43 YY364 pKa = 10.37 ALDD367 pKa = 5.16 FINPDD372 pKa = 2.91 HH373 pKa = 7.35 PYY375 pKa = 10.96 VVIQTTKK382 pKa = 9.81 GPQSRR387 pKa = 11.84 PVSLYY392 pKa = 8.54 YY393 pKa = 10.22 YY394 pKa = 10.34 RR395 pKa = 11.84 KK396 pKa = 9.79 IYY398 pKa = 10.52 CDD400 pKa = 3.28 TLKK403 pKa = 10.91 DD404 pKa = 3.58 EE405 pKa = 4.98 KK406 pKa = 10.99 GQNIYY411 pKa = 10.61 VLNSRR416 pKa = 11.84 GIDD419 pKa = 3.08 MRR421 pKa = 11.84 MRR423 pKa = 11.84 NLSKK427 pKa = 10.66 EE428 pKa = 4.06 LTKK431 pKa = 11.13 LIDD434 pKa = 3.56 DD435 pKa = 3.88 TRR437 pKa = 11.84 KK438 pKa = 10.16 VIQLFRR444 pKa = 11.84 DD445 pKa = 3.56 DD446 pKa = 3.65 SYY448 pKa = 11.84 KK449 pKa = 11.04 AEE451 pKa = 4.56 FYY453 pKa = 11.22 ANQRR457 pKa = 11.84 EE458 pKa = 4.66 YY459 pKa = 11.34 LFHH462 pKa = 7.58 PLNKK466 pKa = 10.06 YY467 pKa = 7.57 NTSLGDD473 pKa = 3.0 IWNDD477 pKa = 2.72 IHH479 pKa = 9.14 SDD481 pKa = 3.21 EE482 pKa = 5.36 DD483 pKa = 4.81 FDD485 pKa = 4.12 MKK487 pKa = 11.13 LNNIEE492 pKa = 4.15 NKK494 pKa = 10.42 LYY496 pKa = 10.69 DD497 pKa = 3.43 YY498 pKa = 11.05 AIYY501 pKa = 10.46 KK502 pKa = 9.88 KK503 pKa = 9.61 IYY505 pKa = 7.31 EE506 pKa = 4.12 WRR508 pKa = 11.84 SYY510 pKa = 10.32 RR511 pKa = 11.84 QDD513 pKa = 3.25 GDD515 pKa = 4.07 SVSVIDD521 pKa = 4.41 PEE523 pKa = 4.08 YY524 pKa = 10.83 DD525 pKa = 3.49 YY526 pKa = 11.86 SCFLHH531 pKa = 7.1 SPALDD536 pKa = 3.47 CSYY539 pKa = 10.92 LPNGFDD545 pKa = 5.46 DD546 pKa = 4.5 ISDD549 pKa = 4.16 SEE551 pKa = 4.31 TSKK554 pKa = 11.34 YY555 pKa = 11.01 NLYY558 pKa = 10.27 CDD560 pKa = 3.7 HH561 pKa = 7.49 PRR563 pKa = 11.84 FKK565 pKa = 11.16 CNIGLFTLLDD575 pKa = 3.82 CMVDD579 pKa = 3.34 YY580 pKa = 11.37 AFWQKK585 pKa = 11.33 DD586 pKa = 2.97 KK587 pKa = 11.32 AKK589 pKa = 9.91 RR590 pKa = 11.84 RR591 pKa = 11.84 RR592 pKa = 11.84 YY593 pKa = 8.17 EE594 pKa = 3.52 EE595 pKa = 3.8 RR596 pKa = 11.84 NKK598 pKa = 9.71 VRR600 pKa = 11.84 KK601 pKa = 7.8 QQIVEE606 pKa = 4.32 KK607 pKa = 10.54 ISTFFNN613 pKa = 3.59
Molecular weight: 73.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.517
IPC2_protein 5.69
IPC_protein 5.728
Toseland 5.995
ProMoST 6.059
Dawson 5.97
Bjellqvist 5.995
Wikipedia 5.97
Rodwell 5.944
Grimsley 6.084
Solomon 5.97
Lehninger 5.957
Nozaki 6.224
DTASelect 6.415
Thurlkill 6.389
EMBOSS 6.351
Sillero 6.326
Patrickios 3.389
IPC_peptide 5.982
IPC2_peptide 6.275
IPC2.peptide.svr19 6.322
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1669
107
613
417.3
47.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.531 ± 2.0
1.558 ± 0.669
7.849 ± 0.824
5.632 ± 0.952
4.913 ± 0.638
5.692 ± 1.04
1.558 ± 0.529
5.153 ± 0.22
6.89 ± 0.992
7.789 ± 0.365
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.397 ± 0.167
5.932 ± 0.418
2.876 ± 0.378
4.374 ± 1.049
4.374 ± 0.542
8.089 ± 0.862
5.812 ± 0.806
5.153 ± 0.448
1.198 ± 0.116
6.231 ± 1.397
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here