Terrapene carolina triunguis (Three-toed box turtle)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Testudinata;

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 31610 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A674IAS5|A0A674IAS5_TERCA Isoform of A0A674IDL1 Pyroglutamylated RFamide peptide receptor OS=Terrapene carolina triunguis OX=2587831 GN=QRFPR PE=3 SV=1
MM1 pKa = 7.8DD2 pKa = 6.08LDD4 pKa = 4.27GDD6 pKa = 4.52GALSMYY12 pKa = 9.61EE13 pKa = 3.73LEE15 pKa = 4.45YY16 pKa = 10.36FYY18 pKa = 11.18EE19 pKa = 4.28EE20 pKa = 4.1QCQKK24 pKa = 10.41LDD26 pKa = 3.43NMAIEE31 pKa = 4.43PLPFEE36 pKa = 5.65DD37 pKa = 5.58CLCQMLDD44 pKa = 3.86LVKK47 pKa = 9.9PQCDD51 pKa = 3.5EE52 pKa = 4.87KK53 pKa = 10.76ITLHH57 pKa = 6.82DD58 pKa = 4.21LKK60 pKa = 10.72KK61 pKa = 10.77CKK63 pKa = 9.59LANVFFDD70 pKa = 3.35TFFNIEE76 pKa = 4.21KK77 pKa = 10.75YY78 pKa = 10.42LDD80 pKa = 3.91HH81 pKa = 6.33EE82 pKa = 4.49QKK84 pKa = 10.92DD85 pKa = 3.89QFSMLRR91 pKa = 11.84DD92 pKa = 3.63AEE94 pKa = 4.31NEE96 pKa = 4.08SQEE99 pKa = 3.73ISDD102 pKa = 3.8WEE104 pKa = 4.17KK105 pKa = 11.0YY106 pKa = 9.66AAEE109 pKa = 4.43EE110 pKa = 3.86YY111 pKa = 10.46DD112 pKa = 3.29ILVAEE117 pKa = 4.98EE118 pKa = 4.11AASDD122 pKa = 3.48QWNDD126 pKa = 2.89GSSISTYY133 pKa = 8.97MANEE137 pKa = 4.03PFF139 pKa = 3.75

Molecular weight: 16.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A674IP84|A0A674IP84_TERCA Low-density lipoprotein receptor-related protein OS=Terrapene carolina triunguis OX=2587831 GN=LRP5 PE=3 SV=1
II1 pKa = 6.73VSNVFPLSFHH11 pKa = 6.42PQSSHH16 pKa = 4.22KK17 pKa = 8.73TFKK20 pKa = 10.34IKK22 pKa = 10.6RR23 pKa = 11.84FLAKK27 pKa = 9.68KK28 pKa = 9.58QKK30 pKa = 8.69QNRR33 pKa = 11.84PIPQWIRR40 pKa = 11.84MKK42 pKa = 9.89TGNKK46 pKa = 8.61IRR48 pKa = 11.84YY49 pKa = 7.09NSKK52 pKa = 8.3RR53 pKa = 11.84RR54 pKa = 11.84HH55 pKa = 3.95WRR57 pKa = 11.84RR58 pKa = 11.84TKK60 pKa = 10.83LGLL63 pKa = 3.67

Molecular weight: 7.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21342

10268

31610

18936447

22

7979

599.1

67.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.529 ± 0.013

2.354 ± 0.016

4.912 ± 0.009

7.033 ± 0.017

3.769 ± 0.009

6.157 ± 0.014

2.572 ± 0.006

4.895 ± 0.012

6.032 ± 0.015

9.851 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.248 ± 0.005

4.043 ± 0.008

5.562 ± 0.016

4.776 ± 0.012

5.358 ± 0.011

8.27 ± 0.015

5.41 ± 0.009

6.106 ± 0.011

1.226 ± 0.005

2.888 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski